Restriction map for
pFN21AB7196
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# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AfaI AflII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshBI
PvuI RsaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AclI 554 AACGTT AA^CGTT
AflIII 984 ACGTGT A^C[GA][CT]GT
Alw44I 1054 GTGCAC G^TGCAC
AlwNI 1043 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1054 GTGCAC G^TGCAC
Asp700I 552 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BclI 803 TGATCA T^GATCA
BpmI 57 CTGGAG CTGGAG(16/14)
BsaBI 762 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1001 ACCGGA [AT]^CCGG[AT]
BspDI 759 ATCGAT AT^CGAT
BspHI 383 TCATGA T^CATGA
BssSI 1057 CACGAG CACGAG(-5/-1)
ClaI 759 ATCGAT AT^CGAT
DraIII 485 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 453 CCTTGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 841 GAATTC G^AATTC
FbaI 803 TGATCA T^GATCA
HgaI 387 GACGC GACGC(5/10)
MamI 762 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflMI 109 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1394 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 1222 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 554 AACGTT AA^CGTT
PstI 869 CTGCAG CTGCA^G
RcaI 383 TCATGA T^CATGA
SacI 66 GAGCTC GAGCT^C
SfaNI 1045 GATGC GCATC(5/9)
SmaI 678 CCCGGG CCC^GGG
SphI 1013 GCATGC GCATG^C
Van91I 109 CCAGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 497 CCACTTTCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 678 CCCGGG C^CCGGG
XmaI 678 CCCGGG C^CCGGG
XmnI 552 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AvaI 678 CCCGGG C^[CT]CG[GA]G
779 CTCGGG
BamHI 3 GGATCC G^GATCC
635 GGATCC
BanII 66 GAGCTC G[GA]GC[CT]^C
1005 GAGCCC
BcnI 678 CCCGG CC^[GC]GG
679 CCGGG
BsiYI 109 CCAGTGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BslI 109 CCAGTGATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
453 CCTTGTGGAGG
BsoBI 678 CCCGGG C^[CT]CG[GA]G
779 CTCGGG
BsrDI 344 GCAATG GCAATG(2/0)
524 GCAATG
BsrI 303 ACTGG ACTGG(1/-1)
109 CCAGT
DdeI 916 CTGAG C^T[ACGT]AG
1284 CTCAG
DraII 1313 AGGGCCT [GA]G^G[ACGT]CC[CT]
1394 AGGACCT
EcoO109I 1313 AGGGCCT [GA]G^G[ACGT]CC[CT]
1394 AGGACCT
EcoT14I 476 CCAAGG C^C[AT][AT]GG
1145 CCAAGG
HinfI 332 GAATC G^A[ACGT]TC
757 GAATC
HphI 246 GGTGA GGTGA(8/7)
484 TCACC
NciI 678 CCCGG CC^[GC]GG
679 CCGGG
NspI 1013 GCATGC [GA]CATG^[CT]
1031 ACATGC
PvuII 872 CAGCTG CAG^CTG
1450 CAGCTG
SfcI 127 CTACAG C^T[GA][CT]AG
869 CTGCAG
StyI 476 CCAAGG C^C[AT][AT]GG
1145 CCAAGG
TfiI 332 GAATC G^A[AT]TC
757 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BmyI BsaJI
BseRI BsiHKAI BsmAI BsmFI Bsp1286I
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DpnII EarI EcoRII FauI
Fnu4HI FokI HaeIII HapII HpaII
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII