(Circular) (Six-base) MAPSORT of: pFN21AB7196.seq Check: 9267 from: 1 to: 6175 pFN21AB7196 6175 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 2560 4302 4675 1563 Size: 997 1742 373 3063 Fragments arranged by size: 3063 1742 997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2966 4238 5622 5890 1632 Size: 1334 1272 1384 268 1917 Fragments arranged by size: 1917 1384 1334 1272 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 2989 5074 6051 1203 Size: 1786 2085 977 1327 Fragments arranged by size: 2085 1786 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 3182 1166 Size: 2016 4159 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2246 1184 Size: 1062 5113 AlwNI CAG_nnn'CTG Cuts at: 3053 3918 5490 3053 Size: 865 1572 3738 Fragments arranged by size: 3738 1572 865 ApaLI G'TGCA_C Cuts at: 3059 4232 5388 3059 Size: 1173 1156 3846 Fragments arranged by size: 3846 1173 1156 ApoI r'AATT_y Cuts at: 1493 2401 2846 3128 3203 3256 3484 3710 3862 Size: 908 445 282 75 53 228 226 152 Cuts at: 3862 5971 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 908 445 282 228 226 152 75 53 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 2683 2784 3500 1870 Size: 73 740 101 716 4545 Fragments arranged by size: 4545 740 716 101 73 BamHI G'GATC_C Cuts at: 2008 2640 3505 3872 2008 Size: 632 865 367 4311 Fragments arranged by size: 4311 865 632 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2075 3014 3494 1391 Size: 684 939 480 4072 Fragments arranged by size: 4072 939 684 480 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 2808 1548 Size: 1260 4915 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 3063 3158 4236 5392 1790 Size: 1273 95 1078 1156 2573 Fragments arranged by size: 2573 1273 1156 1078 95 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2108 3906 4864 Size: 416 53 83 399 24 691 1798 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1798 1753 958 691 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2105 2946 2105 Size: 841 5334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2083 4835 1239 Size: 844 2752 2579 Fragments arranged by size: 2752 2579 844 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 2771 6169 2771 Size: 3398 2777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3006 4608 5006 5280 5427 5838 3006 Size: 1602 398 274 147 411 3343 Fragments arranged by size: 3343 1602 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2097 2294 2434 2480 1186 Size: 911 197 140 46 4881 Fragments arranged by size: 4881 911 197 140 46 BseYI C'CCAG_C Cuts at: 2894 5378 5874 2894 Size: 2484 496 3195 Fragments arranged by size: 3195 2484 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5602 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2031 2075 2524 3063 4236 4321 5392 2031 Size: 44 449 539 1173 85 1071 2814 Fragments arranged by size: 2814 1173 1071 539 449 85 44 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2031 2075 2524 3014 3063 3158 3494 Size: 399 241 44 449 490 49 95 336 Cuts at: 3494 4236 4321 5392 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 490 449 399 336 241 95 85 49 44 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 2388 4064 5794 2388 Size: 1676 1730 2769 Fragments arranged by size: 2769 1730 1676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3530 844 Size: 2686 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2356 2536 4670 4844 65 Size: 1183 1108 180 2134 174 1396 Fragments arranged by size: 2134 1396 1183 1108 180 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 3062 4235 5247 3062 Size: 1173 1012 3990 Fragments arranged by size: 3990 1173 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2008 2370 2640 3505 3872 4257 4274 5726 Size: 36 362 270 865 367 385 17 1452 Cuts at: 5726 5812 5824 6170 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 865 385 367 362 346 270 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2871 3117 3691 4485 4505 2871 Size: 246 574 794 20 4541 Fragments arranged by size: 4541 794 574 246 20 ClaI AT'CG_AT Cuts at: 1554 2765 1554 Size: 1211 4964 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2653 2688 529 Size: 35 2089 35 4016 Fragments arranged by size: 4016 2089 35 35 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DraIII CAC_nnn'GTG Cuts at: 2495 2495 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140 Size: 54 1425 50 275 18 1792 889 1629 Cuts at: 6140 8 Size: 43 Fragments arranged by size: 1792 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2287 2411 2973 4105 1353 Size: 934 124 562 1132 3423 Fragments arranged by size: 3423 1132 934 562 124 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2073 2073 Size: 6175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2083 2966 4238 4835 5622 5890 1239 Size: 393 451 883 1272 597 787 268 1524 Fragments arranged by size: 1524 1272 883 787 597 451 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2462 2462 Size: 6175 EcoO109I rG'GnC_Cy Cuts at: 3319 3400 3319 Size: 81 6094 EcoRI G'AATT_C Cuts at: 2846 3484 3862 2846 Size: 638 378 5159 Fragments arranged by size: 5159 638 378 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2322 2354 2916 2948 4816 Size: 32 135 32 750 32 562 32 1868 Cuts at: 4816 4848 4890 4922 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1868 750 562 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3519 3765 669 Size: 988 281 1581 246 3079 Fragments arranged by size: 3079 1581 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5289 5473 1305 Size: 3984 184 2007 Fragments arranged by size: 3984 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2228 2241 3182 4133 4492 4651 518 Size: 648 1062 13 941 951 359 159 2042 Fragments arranged by size: 2042 1062 951 941 648 359 159 13 MspA1I CmG'CkG Cuts at: 1606 2454 2879 3457 3591 4268 5416 5661 1606 Size: 848 425 578 134 677 1148 245 2120 Fragments arranged by size: 2120 1148 848 677 578 425 245 134 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3022 3040 3533 5078 1132 Size: 97 1793 18 493 1545 2229 Fragments arranged by size: 2229 1793 1545 493 97 18 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 2120 1108 Size: 1012 5163 PfoI T'CCnGG_A Cuts at: 2973 2973 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3400 3400 Size: 6175 PshAI GACnn'nnGTC Cuts at: 1613 3231 1613 Size: 1618 4557 PsiI TTA'TAA Cuts at: 2571 3745 2571 Size: 1174 5001 PstI C_TGCA'G Cuts at: 830 2878 3527 830 Size: 2048 649 3478 Fragments arranged by size: 3478 2048 649 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2879 3457 2879 Size: 578 5597 SacI G_AGCT'C Cuts at: 2075 2075 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2132 2874 3523 4658 5339 5530 826 Size: 219 1087 742 649 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 742 681 649 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 2685 3502 1872 Size: 813 817 4545 Fragments arranged by size: 4545 817 813 SmlI C'TyrA_G Cuts at: 820 1017 1943 4279 5180 5442 820 Size: 197 926 2336 901 262 1553 Fragments arranged by size: 2336 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3022 3533 1132 Size: 1890 511 3774 Fragments arranged by size: 3774 1890 511 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 2481 3150 3614 513 Size: 552 939 477 669 464 3074 Fragments arranged by size: 3074 939 669 552 477 464 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2967 4202 4219 4372 4557 5002 325 Size: 1255 1387 1235 17 153 185 445 1498 Fragments arranged by size: 1498 1387 1255 1235 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4421 6133 96 Size: 274 80 33 51 1443 2444 1712 138 Fragments arranged by size: 2444 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2232 2264 6135 6167 2232 Size: 32 3871 32 2240 Fragments arranged by size: 3871 2240 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2509 2509 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 2683 3500 1870 Size: 813 817 4545 Fragments arranged by size: 4545 817 813 XmnI GAAnn'nnTTC Cuts at: 2561 4304 2561 Size: 1743 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI