Restriction map for
pFN21AB7196
(Linear) (Six-base) MAPSORT of: KIBB7196.seq Check: 1343 from: 1 to: 1470
KIBB7196.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AclI AA'CG_TT
Cuts at: 0 555 1470
Size: 555 915
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1470
Size: 961 509
AflIII A'CryG_T
Cuts at: 0 984 1470
Size: 984 486
AleI CACnn'nnGTG
Cuts at: 0 1177 1470
Size: 1177 293
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 1470
Size: 241 1229
AlwNI CAG_nnn'CTG
Cuts at: 0 1048 1470
Size: 1048 422
ApaLI G'TGCA_C
Cuts at: 0 1054 1470
Size: 1054 416
ApoI r'AATT_y
Cuts at: 0 396 841 1123 1198 1251 1470
Size: 396 445 282 75 53 219
Fragments arranged by size:
445 396 282 219 75 53
AvaI C'yCGr_G
Cuts at: 0 678 779 1470
Size: 678 101 691
Fragments arranged by size:
691 678 101
BamHI G'GATC_C
Cuts at: 0 3 635 1470
Size: 3 632 835
Fragments arranged by size:
835 632 3
BanII G_rGCy'C
Cuts at: 0 70 1009 1470
Size: 70 939 461
Fragments arranged by size:
939 461 70
BclI T'GATC_A
Cuts at: 0 803 1470
Size: 803 667
Bme1580I G_kGCm'C
Cuts at: 0 1058 1153 1470
Size: 1058 95 317
Fragments arranged by size:
1058 317 95
BmrI ACTGGGnnnn_n'
Cuts at: 0 103 1470
Size: 103 1367
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 100 941 1470
Size: 100 841 529
Fragments arranged by size:
841 529 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 1470
Size: 78 1392
BsaBI GATnn'nnATC
Cuts at: 0 766 1470
Size: 766 704
BsaWI w'CCGG_w
Cuts at: 0 1001 1470
Size: 1001 469
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 92 289 429 475 1470
Size: 92 197 140 46 995
Fragments arranged by size:
995 197 140 92 46
BseYI C'CCAG_C
Cuts at: 0 889 1470
Size: 889 581
BsiHKAI G_wGCw'C
Cuts at: 0 26 70 519 1058 1470
Size: 26 44 449 539 412
Fragments arranged by size:
539 449 412 44 26
Bsp1286I G_dGCh'C
Cuts at: 0 26 70 519 1009 1058 1153 1470
Size: 26 44 449 490 49 95 317
Fragments arranged by size:
490 449 317 95 49 44 26
BspHI T'CATG_A
Cuts at: 0 383 1470
Size: 383 1087
BsrDI GCAATG_nn'
Cuts at: 0 351 531 1470
Size: 351 180 939
Fragments arranged by size:
939 351 180
BssSI C'ACGA_G
Cuts at: 0 1057 1470
Size: 1057 413
BstYI r'GATC_y
Cuts at: 0 3 365 635 1470
Size: 3 362 270 835
Fragments arranged by size:
835 362 270 3
BtsI GCAGTG_nn'
Cuts at: 0 866 1112 1470
Size: 866 246 358
Fragments arranged by size:
866 358 246
ClaI AT'CG_AT
Cuts at: 0 760 1470
Size: 760 710
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 648 683 1470
Size: 648 35 787
Fragments arranged by size:
787 648 35
DraIII CAC_nnn'GTG
Cuts at: 0 490 1470
Size: 490 980
EarI CTCTTCn'nnn_
Cuts at: 0 282 406 968 1470
Size: 282 124 562 502
Fragments arranged by size:
562 502 282 124
EcoICRI GAG'CTC
Cuts at: 0 68 1470
Size: 68 1402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 961 1470
Size: 78 883 509
Fragments arranged by size:
883 509 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 457 1470
Size: 457 1013
EcoO109I rG'GnC_Cy
Cuts at: 0 1314 1395 1470
Size: 1314 81 75
Fragments arranged by size:
1314 81 75
EcoRI G'AATT_C
Cuts at: 0 841 1470
Size: 841 629
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 317 349 911 943 1470
Size: 317 32 562 32 527
Fragments arranged by size:
562 527 317 32 32
MslI CAynn'nnrTG
Cuts at: 0 223 236 1177 1470
Size: 223 13 941 293
Fragments arranged by size:
941 293 223 13
MspA1I CmG'CkG
Cuts at: 0 449 874 1452 1470
Size: 449 425 578 18
Fragments arranged by size:
578 449 425 18
NspI r_CATG'y
Cuts at: 0 1017 1035 1470
Size: 1017 18 435
Fragments arranged by size:
1017 435 18
PflMI CCAn_nnn'nTGG
Cuts at: 0 115 1470
Size: 115 1355
PfoI T'CCnGG_A
Cuts at: 0 968 1470
Size: 968 502
PpuMI rG'GwC_Cy
Cuts at: 0 1395 1470
Size: 1395 75
PshAI GACnn'nnGTC
Cuts at: 0 1226 1470
Size: 1226 244
PsiI TTA'TAA
Cuts at: 0 566 1470
Size: 566 904
PstI C_TGCA'G
Cuts at: 0 873 1470
Size: 873 597
PvuII CAG'CTG
Cuts at: 0 874 1452 1470
Size: 874 578 18
Fragments arranged by size:
874 578 18
SacI G_AGCT'C
Cuts at: 0 70 1470
Size: 70 1400
SfcI C'TryA_G
Cuts at: 0 127 869 1470
Size: 127 742 601
Fragments arranged by size:
742 601 127
SmaI CCC'GGG
Cuts at: 0 680 1470
Size: 680 790
SphI G_CATG'C
Cuts at: 0 1017 1470
Size: 1017 453
StyI C'CwwG_G
Cuts at: 0 476 1145 1470
Size: 476 669 325
Fragments arranged by size:
669 476 325
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 962 1470
Size: 962 508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 227 259 1470
Size: 227 32 1211
Fragments arranged by size:
1211 227 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 1470
Size: 504 966
XmaI C'CCGG_G
Cuts at: 0 678 1470
Size: 678 792
XmnI GAAnn'nnTTC
Cuts at: 0 556 1470
Size: 556 914
Enzymes that do cut:
AclI AcuI AflIII AleI AlfI AlwNI ApaLI ApoI
AvaI BamHI BanII BclI Bme1580I BmrI BplI BpmI
BsaBI BsaWI BseRI BseYI BsiHKAI Bsp1286I BspHI BsrDI
BssSI BstYI BtsI ClaI CspCI DraIII EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI Hin4I MslI MspA1I NspI
PflMI PfoI PpuMI PshAI PsiI PstI PvuII SacI
SfcI SmaI SphI StyI TaqII TstI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AloI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbsI BbvCI BcgI BciVI BfrBI BglI BglII
BlpI BmgBI BmtI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI DraI DrdI EaeI EagI
EciI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I
XbaI XhoI ZraI