(Circular) (Six-base) MAPSORT of: pFN21AB7194.seq Check: 8416 from: 1 to: 6733 pFN21AB7194 6733 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2653 2653 Size: 6733 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3627 4543 278 Size: 53 83 186 936 2091 916 2468 Fragments arranged by size: 2468 2091 936 916 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 2727 3378 3948 4076 1937 Size: 397 393 651 570 128 4594 Fragments arranged by size: 4594 651 570 397 393 128 Acc65I G'GTAC_C Cuts at: 4054 4054 Size: 6733 AclI AA'CG_TT Cuts at: 1563 4860 5233 1563 Size: 3297 373 3063 Fragments arranged by size: 3297 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2404 3083 4796 6180 6448 1632 Size: 772 679 1713 1384 268 1917 Fragments arranged by size: 1917 1713 1384 772 679 268 AfeI AGC'GCT Cuts at: 2045 2045 Size: 6733 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6536 AflIII A'CryG_T Cuts at: 1203 2722 5632 6609 1203 Size: 1519 2910 977 1327 Fragments arranged by size: 2910 1519 1327 977 AgeI A'CCGG_T Cuts at: 5564 5564 Size: 6733 AhdI GACnn_n'nnGTC Cuts at: 5462 5462 Size: 6733 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6733 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2685 3160 3995 1184 Size: 1501 475 835 3922 Fragments arranged by size: 3922 1501 835 475 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3152 3184 3152 Size: 32 6701 AlwNI CAG_nnn'CTG Cuts at: 2710 2788 3820 4476 6048 2710 Size: 78 1032 656 1572 3395 Fragments arranged by size: 3395 1572 1032 656 78 ApaI G_GGCC'C Cuts at: 3913 4019 3913 Size: 106 6627 ApaLI G'TGCA_C Cuts at: 4790 5946 4790 Size: 1156 5577 ApoI r'AATT_y Cuts at: 1493 3587 4042 4268 4420 6529 1493 Size: 2094 455 226 152 2109 1697 Fragments arranged by size: 2109 2094 1697 455 226 152 AseI AT'TA_AT Cuts at: 160 1032 5287 160 Size: 872 4255 1606 Fragments arranged by size: 4255 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 AvaI C'yCGr_G Cuts at: 1870 1943 2345 3049 4058 1870 Size: 73 402 704 1009 4545 Fragments arranged by size: 4545 1009 704 402 73 BamHI G'GATC_C Cuts at: 4063 4430 4063 Size: 367 6366 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2069 2174 2741 2959 3770 Size: 325 217 627 282 105 567 218 811 Cuts at: 3770 4054 5509 618 Size: 284 1455 1842 Fragments arranged by size: 1842 1455 811 627 567 325 284 282 218 217 105 BanII G_rGCy'C Cuts at: 1391 2654 2762 3410 3913 3960 4019 4052 1391 Size: 1263 108 648 503 47 59 33 4072 Fragments arranged by size: 4072 1263 648 503 108 59 47 33 BbeI G_GCGC'C Cuts at: 2073 2178 3774 2073 Size: 105 1596 5032 Fragments arranged by size: 5032 1596 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2383 2761 2987 3798 928 Size: 971 484 378 226 811 3863 Fragments arranged by size: 3863 971 811 484 378 226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2429 2463 3748 3782 4924 4958 1903 Size: 34 492 34 1285 34 1142 34 3678 Fragments arranged by size: 3678 1285 1142 492 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4625 5835 4625 Size: 1210 5523 BclI T'GATC_A Cuts at: 1548 1548 Size: 6733 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 2142 2456 Size: 107 122 71 631 605 395 75 314 Cuts at: 2456 2678 2739 5344 136 Size: 222 61 2605 1525 Fragments arranged by size: 2605 1525 631 605 395 314 222 122 107 75 71 61 BglII A'GATC_T Cuts at: 3183 6728 3183 Size: 3545 3188 BlpI GC'TnA_GC Cuts at: 4150 4150 Size: 6733 Bme1580I G_kGCm'C Cuts at: 1790 2108 2964 3913 4008 4019 4794 5950 1790 Size: 318 856 949 95 11 775 1156 2573 Fragments arranged by size: 2573 1156 949 856 775 318 95 11 BmgBI CAC'GTC Cuts at: 1318 2320 2676 2725 2970 1318 Size: 1002 356 49 245 5081 Fragments arranged by size: 5081 1002 356 245 49 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2219 4464 5422 Size: 416 53 83 399 24 802 2245 958 Cuts at: 5422 442 Size: 1753 Fragments arranged by size: 2245 1753 958 802 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6733 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3231 3655 3231 Size: 424 6309 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2138 2767 3324 5393 1239 Size: 899 629 557 2069 2579 Fragments arranged by size: 2579 2069 899 629 557 Bpu10I CC'TnA_GC Cuts at: 3954 3954 Size: 6733 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4858 5723 6021 4858 Size: 865 298 5570 Fragments arranged by size: 5570 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5396 882 Size: 617 3897 2219 Fragments arranged by size: 3897 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2518 2557 3336 4524 493 Size: 711 1314 39 779 1188 2702 Fragments arranged by size: 2702 1314 1188 779 711 39 BsaBI GATnn'nnATC Cuts at: 2483 3188 6727 2483 Size: 705 3539 2489 Fragments arranged by size: 3539 2489 705 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2070 2175 3624 3771 Size: 53 83 186 936 537 105 1449 147 Cuts at: 3771 4540 4922 5557 275 Size: 769 382 635 1451 Fragments arranged by size: 1451 1449 936 769 635 537 382 186 147 105 83 53 BsaWI w'CCGG_w Cuts at: 3192 5166 5564 5838 5985 6396 3192 Size: 1974 398 274 147 411 3529 Fragments arranged by size: 3529 1974 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3152 3182 1178 Size: 30 165 30 1749 30 4729 Fragments arranged by size: 4729 1749 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2008 2821 3160 3700 1186 Size: 822 813 339 540 4219 Fragments arranged by size: 4219 822 813 540 339 BseYI C'CCAG_C Cuts at: 2046 3031 3227 3921 3968 5936 6432 2046 Size: 985 196 694 47 1968 496 2347 Fragments arranged by size: 2347 1968 985 694 496 196 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3089 1163 Size: 573 1353 4807 Fragments arranged by size: 4807 1353 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2208 2252 2731 3768 4183 4944 Size: 275 186 207 44 479 1037 415 761 Cuts at: 4944 5972 1540 Size: 1028 2301 Fragments arranged by size: 2301 1037 1028 761 479 415 275 207 186 44 BsiHKAI G_wGCw'C Cuts at: 3960 4794 4879 5950 3960 Size: 834 85 1071 4743 Fragments arranged by size: 4743 1071 834 85 BsmI GAATG_Cn' Cuts at: 4244 4337 4244 Size: 93 6640 BsmBI CGTCTCn'nnnn_ Cuts at: 2281 3736 2281 Size: 1455 5278 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2654 2762 2964 3410 3913 3960 Size: 399 318 546 108 202 446 503 47 Cuts at: 3960 4008 4019 4052 4794 4879 5950 1391 Size: 48 11 33 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 546 503 446 399 318 202 108 85 48 47 33 11 BspEI T'CCGG_A Cuts at: 3192 6396 3192 Size: 3204 3529 BspHI T'CATG_A Cuts at: 3441 4622 6352 3441 Size: 1181 1730 3822 Fragments arranged by size: 3822 1730 1181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2653 4088 844 Size: 1809 1435 3489 Fragments arranged by size: 3489 1809 1435 BsrBI CCG'CTC Cuts at: 1394 1823 3355 4620 1394 Size: 429 1532 1265 3507 Fragments arranged by size: 3507 1532 1265 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5228 5402 65 Size: 1183 3980 174 1396 Fragments arranged by size: 3980 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2254 2357 2537 3099 5377 5564 1700 Size: 110 444 103 180 562 2278 187 2869 Fragments arranged by size: 2869 2278 562 444 187 180 110 103 BsrGI T'GTAC_A Cuts at: 96 2802 96 Size: 2706 4027 BssHII G'CGCG_C Cuts at: 1925 2148 2309 1925 Size: 223 161 6349 Fragments arranged by size: 6349 223 161 BssSI C'ACGA_G Cuts at: 4793 5805 4793 Size: 1012 5721 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6733 BstBI TT'CG_AA Cuts at: 5537 5537 Size: 6733 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6733 BstXI CCAn_nnnn'nTGG Cuts at: 3704 3704 Size: 6733 BstYI r'GATC_y Cuts at: 1972 2706 3105 3183 4063 4430 4815 4832 6284 Size: 734 399 78 880 367 385 17 1452 Cuts at: 6284 6370 6382 6728 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 880 734 399 385 367 346 86 78 17 12 Bsu36I CC'TnA_GG Cuts at: 2711 2711 Size: 6733 BtgI C'CryG_G Cuts at: 513 1065 2004 2010 2246 2460 2631 2763 513 Size: 552 939 6 236 214 171 132 4483 Fragments arranged by size: 4483 939 552 236 214 171 132 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2149 2971 487 Size: 683 56 923 822 4249 Fragments arranged by size: 4249 923 822 683 56 BtsI GCAGTG_nn' Cuts at: 3413 3940 4249 5043 5063 3413 Size: 527 309 794 20 5083 Fragments arranged by size: 5083 794 527 309 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6733 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6698 DraI TTT'AAA Cuts at: 4037 4037 Size: 6733 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2273 5740 809 Size: 507 957 3467 1802 Fragments arranged by size: 3467 1802 957 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2094 2205 Size: 54 1425 50 275 18 177 87 111 Cuts at: 2205 2249 2457 2679 3576 4180 5069 6698 8 Size: 44 208 222 897 604 889 1629 43 Fragments arranged by size: 1629 1425 897 889 604 275 222 208 177 111 87 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2205 2249 4180 1537 Size: 275 393 44 1931 4090 Fragments arranged by size: 4090 1931 393 275 44 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 3345 3398 3965 4663 1353 Size: 1124 868 53 567 698 3423 Fragments arranged by size: 3423 1124 868 698 567 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2141 2513 2524 5320 5694 5840 Size: 1064 138 552 372 11 2796 374 146 Cuts at: 5840 387 Size: 1280 Fragments arranged by size: 2796 1280 1064 552 374 372 146 138 11 EcoICRI GAG'CTC Cuts at: 3958 3958 Size: 6733 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2138 2404 2767 3083 3324 4796 5393 Size: 393 506 266 363 316 241 1472 597 Cuts at: 5393 6180 6448 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1472 787 597 506 393 363 316 268 266 241 EcoO109I rG'GnC_Cy Cuts at: 2183 3199 3466 3909 4015 4016 2183 Size: 1016 267 443 106 1 4900 Fragments arranged by size: 4900 1016 443 267 106 1 EcoRI G'AATT_C Cuts at: 4042 4420 4042 Size: 378 6355 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2125 Size: 6733 FspI TGC'GCA Cuts at: 3668 5239 3668 Size: 1571 5162 FspAI rTGC'GCAy Cuts at: 3668 3668 Size: 6733 HaeII r_GCGC'y Cuts at: 1678 2047 2073 2178 3774 5880 1678 Size: 369 26 105 1596 2106 2531 Fragments arranged by size: 2531 2106 1596 369 105 26 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3356 3388 3733 3765 4013 Size: 32 135 32 1784 32 345 32 248 Cuts at: 4013 4045 5374 5406 5448 5480 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1784 1329 345 248 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2728 3379 3949 4077 4323 669 Size: 988 281 790 651 570 128 246 3079 Fragments arranged by size: 3079 988 790 651 570 281 246 128 HindIII A'AGCT_T Cuts at: 748 748 Size: 6733 HpaI GTT'AAC Cuts at: 4323 4323 Size: 6733 KasI G'GCGC_C Cuts at: 2069 2174 3770 2069 Size: 105 1596 5032 Fragments arranged by size: 5032 1596 105 KpnI G_GTAC'C Cuts at: 4058 4058 Size: 6733 MfeI C'AATT_G Cuts at: 4332 4332 Size: 6733 MluI A'CGCG_T Cuts at: 6609 6609 Size: 6733 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2259 5847 6031 1305 Size: 954 3588 184 2007 Fragments arranged by size: 3588 2007 954 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 2459 3578 10 Size: 54 1425 343 264 363 1119 3165 Fragments arranged by size: 3165 1425 1119 363 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 3142 3836 4691 5050 5209 518 Size: 648 1976 694 855 359 159 2042 Fragments arranged by size: 2042 1976 855 694 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2012 2248 3035 3520 3808 3850 3885 3997 Size: 406 236 787 485 288 42 35 112 Cuts at: 3997 4149 4826 5974 6219 1606 Size: 152 677 1148 245 2120 Fragments arranged by size: 2120 1148 787 677 485 406 288 245 236 152 112 42 35 NaeI GCC'GGC Cuts at: 2539 2539 Size: 6733 NarI GG'CG_CC Cuts at: 2070 2175 3771 2070 Size: 105 1596 5032 Fragments arranged by size: 5032 1596 105 NcoI C'CATG_G Cuts at: 513 1065 2004 2631 513 Size: 552 939 627 4615 Fragments arranged by size: 4615 939 627 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6733 NgoMIV G'CCGG_C Cuts at: 2537 2537 Size: 6733 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6733 NotI GC'GGCC_GC Cuts at: 2249 4180 2249 Size: 1931 4802 NspI r_CATG'y Cuts at: 1132 1229 3433 3565 3841 4091 5636 1132 Size: 97 2204 132 276 250 1545 2229 Fragments arranged by size: 2229 2204 1545 276 250 132 97 PasI CC'CwG_GG Cuts at: 1348 2028 1348 Size: 680 6053 PciI A'CATG_T Cuts at: 5632 5632 Size: 6733 PflMI CCAn_nnn'nTGG Cuts at: 1108 2361 3820 1108 Size: 1253 1459 4021 Fragments arranged by size: 4021 1459 1253 PfoI T'CCnGG_A Cuts at: 3301 3301 Size: 6733 PmeI GTTT'AAAC Cuts at: 4037 4037 Size: 6733 PmlI CAC'GTG Cuts at: 3336 3336 Size: 6733 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3152 3184 4802 4834 6315 6347 3152 Size: 32 1618 32 1481 32 3538 Fragments arranged by size: 3538 1618 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2183 3466 2183 Size: 1283 5450 PshAI GACnn'nnGTC Cuts at: 1613 2972 1613 Size: 1359 5374 PsiI TTA'TAA Cuts at: 4303 4303 Size: 6733 PspOMI G'GGCC_C Cuts at: 3909 4015 3909 Size: 106 6627 PspXI vC'TCGA_Gb Cuts at: 2345 2345 Size: 6733 PstI C_TGCA'G Cuts at: 830 2706 3684 4085 830 Size: 1876 978 401 3478 Fragments arranged by size: 3478 1876 978 401 PvuI CG_AT'CG Cuts at: 2001 3768 2001 Size: 1767 4966 PvuII CAG'CTG Cuts at: 3520 3808 3850 3997 3520 Size: 288 42 147 6256 Fragments arranged by size: 6256 288 147 42 SacI G_AGCT'C Cuts at: 3960 3960 Size: 6733 SacII CC_GC'GG Cuts at: 2013 2249 2013 Size: 236 6497 SalI G'TCGA_C Cuts at: 1936 2726 3377 3947 4075 1936 Size: 790 651 570 128 4594 Fragments arranged by size: 4594 790 651 570 128 SapI GCTCTTCn'nnn_ Cuts at: 3345 3398 3965 3345 Size: 53 567 6113 Fragments arranged by size: 6113 567 53 SbfI CC_TGCA'GG Cuts at: 4085 4085 Size: 6733 ScaI AGT'ACT Cuts at: 3569 3638 4981 6693 3569 Size: 69 1343 1712 3609 Fragments arranged by size: 3609 1712 1343 69 SfcI C'TryA_G Cuts at: 826 1045 2284 2702 3680 4081 5216 5897 6088 Size: 219 1239 418 978 401 1135 681 191 Cuts at: 6088 826 Size: 1471 Fragments arranged by size: 1471 1239 1135 978 681 418 401 219 191 SfoI GGC'GCC Cuts at: 2071 2176 3772 2071 Size: 105 1596 5032 Fragments arranged by size: 5032 1596 105 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6733 SgrAI Cr'CCGG_yG Cuts at: 1700 2254 1700 Size: 554 6179 SmaI CCC'GGG Cuts at: 1872 4060 1872 Size: 2188 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2345 4837 5738 6000 820 Size: 197 926 402 2492 901 262 1553 Fragments arranged by size: 2492 1553 926 901 402 262 197 SnaBI TAC'GTA Cuts at: 493 4524 493 Size: 4031 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6733 SphI G_CATG'C Cuts at: 1132 3433 3565 4091 1132 Size: 2301 132 526 3774 Fragments arranged by size: 3774 2301 526 132 SspI AAT'ATT Cuts at: 5 52 4657 5 Size: 47 4605 2081 Fragments arranged by size: 4605 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6733 StyI C'CwwG_G Cuts at: 513 1065 2004 2239 2631 2655 4172 513 Size: 552 939 235 392 24 1517 3074 Fragments arranged by size: 3074 1517 939 552 392 235 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2330 3241 3514 3521 3935 4760 4777 Size: 1255 750 911 273 7 414 825 17 Cuts at: 4777 4930 5115 5560 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 911 825 750 445 414 273 185 153 17 7 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 3567 3636 Size: 274 80 33 51 1443 825 765 69 Cuts at: 3636 4979 6691 96 Size: 1343 1712 138 Fragments arranged by size: 1712 1443 1343 825 765 274 138 80 69 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6693 6725 6693 Size: 32 6701 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6733 XbaI T'CTAG_A Cuts at: 4069 4069 Size: 6733 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3247 3247 Size: 6733 XhoI C'TCGA_G Cuts at: 1943 2345 1943 Size: 402 6331 XmaI C'CCGG_G Cuts at: 1870 4058 1870 Size: 2188 4545 XmnI GAAnn'nnTTC Cuts at: 2369 3092 3587 4862 2369 Size: 723 495 1275 4240 Fragments arranged by size: 4240 1275 723 495 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3625 4541 276 Size: 53 83 186 936 2091 916 2468 Fragments arranged by size: 2468 2091 936 916 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I DraIII EcoNI EcoRV FseI NruI NsiI PacI PsrI RsrII SanDI SexAI SfiI SrfI SwaI