Restriction map for
pFN21AB7194
(Linear) (Six-base) MAPSORT of: KIBB7194.seq Check: 1949 from: 1 to: 2028
KIBB7194.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 648 2028
Size: 648 1380
AatII G_ACGT'C
Cuts at: 0 1622 2028
Size: 1622 406
AccI GT'mk_AC
Cuts at: 0 329 722 1373 1943 2028
Size: 329 393 651 570 85
Fragments arranged by size:
651 570 393 329 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 399 1078 2028
Size: 399 679 950
Fragments arranged by size:
950 679 399
AfeI AGC'GCT
Cuts at: 0 40 2028
Size: 40 1988
AflIII A'CryG_T
Cuts at: 0 717 2028
Size: 717 1311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 680 1155 1990 2028
Size: 680 475 835 38
Fragments arranged by size:
835 680 475 38
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 2028
Size: 1147 32 849
Fragments arranged by size:
1147 849 32
AlwNI CAG_nnn'CTG
Cuts at: 0 705 783 1815 2028
Size: 705 78 1032 213
Fragments arranged by size:
1032 705 213 78
ApaI G_GGCC'C
Cuts at: 0 1908 2014 2028
Size: 1908 106 14
Fragments arranged by size:
1908 106 14
ApoI r'AATT_y
Cuts at: 0 1582 2028
Size: 1582 446
AvaI C'yCGr_G
Cuts at: 0 340 1044 2028
Size: 340 704 984
Fragments arranged by size:
984 704 340
BanI G'GyrC_C
Cuts at: 0 64 169 736 954 1765 2028
Size: 64 105 567 218 811 263
Fragments arranged by size:
811 567 263 218 105 64
BanII G_rGCy'C
Cuts at: 0 649 757 1405 1908 1955 2014 2028
Size: 649 108 648 503 47 59 14
Fragments arranged by size:
649 648 503 108 59 47 14
BbeI G_GCGC'C
Cuts at: 0 68 173 1769 2028
Size: 68 105 1596 259
Fragments arranged by size:
1596 259 105 68
BbsI GAAGACnn'nnnn_
Cuts at: 0 378 756 982 1793 2028
Size: 378 378 226 811 235
Fragments arranged by size:
811 378 378 235 226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 424 458 1743 1777 2028
Size: 424 34 1285 34 251
Fragments arranged by size:
1285 424 251 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 62 137 451 673 734 2028
Size: 62 75 314 222 61 1294
Fragments arranged by size:
1294 314 222 75 62 61
BglII A'GATC_T
Cuts at: 0 1178 2028
Size: 1178 850
Bme1580I G_kGCm'C
Cuts at: 0 103 959 1908 2003 2014 2028
Size: 103 856 949 95 11 14
Fragments arranged by size:
949 856 103 95 14 11
BmgBI CAC'GTC
Cuts at: 0 315 671 720 965 2028
Size: 315 356 49 245 1063
Fragments arranged by size:
1063 356 315 245 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 214 2028
Size: 214 1814
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1226 1650 2028
Size: 1226 424 378
Fragments arranged by size:
1226 424 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 762 1319 2028
Size: 133 629 557 709
Fragments arranged by size:
709 629 557 133
Bpu10I CC'TnA_GC
Cuts at: 0 1949 2028
Size: 1949 79
BsaAI yAC'GTr
Cuts at: 0 513 552 1331 2028
Size: 513 39 779 697
Fragments arranged by size:
779 697 513 39
BsaBI GATnn'nnATC
Cuts at: 0 478 1183 2028
Size: 478 705 845
Fragments arranged by size:
845 705 478
BsaHI Gr'CG_yC
Cuts at: 0 65 170 1619 1766 2028
Size: 65 105 1449 147 262
Fragments arranged by size:
1449 262 147 105 65
BsaWI w'CCGG_w
Cuts at: 0 1187 2028
Size: 1187 841
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1147 1177 2028
Size: 1147 30 851
Fragments arranged by size:
1147 851 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3 816 1155 1695 2028
Size: 3 813 339 540 333
Fragments arranged by size:
813 540 339 333 3
BseYI C'CCAG_C
Cuts at: 0 41 1026 1222 1916 1963 2028
Size: 41 985 196 694 47 65
Fragments arranged by size:
985 694 196 65 47 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 2028
Size: 1084 944
BsiEI CG_ry'CG
Cuts at: 0 203 247 726 1763 2028
Size: 203 44 479 1037 265
Fragments arranged by size:
1037 479 265 203 44
BsiHKAI G_wGCw'C
Cuts at: 0 1955 2028
Size: 1955 73
BsmBI CGTCTCn'nnnn_
Cuts at: 0 276 1731 2028
Size: 276 1455 297
Fragments arranged by size:
1455 297 276
Bsp1286I G_dGCh'C
Cuts at: 0 103 649 757 959 1405 1908 1955 2003
Size: 103 546 108 202 446 503 47 48
Cuts at: 2003 2014 2028
Size: 11 14
Fragments arranged by size:
546 503 446 202 108 103 48 47
14 11
BspEI T'CCGG_A
Cuts at: 0 1187 2028
Size: 1187 841
BspHI T'CATG_A
Cuts at: 0 1436 2028
Size: 1436 592
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 2028
Size: 648 1380
BsrBI CCG'CTC
Cuts at: 0 1350 2028
Size: 1350 678
BsrFI r'CCGG_y
Cuts at: 0 249 352 532 1094 2028
Size: 249 103 180 562 934
Fragments arranged by size:
934 562 249 180 103
BsrGI T'GTAC_A
Cuts at: 0 797 2028
Size: 797 1231
BssHII G'CGCG_C
Cuts at: 0 143 304 2028
Size: 143 161 1724
Fragments arranged by size:
1724 161 143
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1699 2028
Size: 1699 329
BstYI r'GATC_y
Cuts at: 0 701 1100 1178 2028
Size: 701 399 78 850
Fragments arranged by size:
850 701 399 78
Bsu36I CC'TnA_GG
Cuts at: 0 706 2028
Size: 706 1322
BtgI C'CryG_G
Cuts at: 0 5 241 455 626 758 2028
Size: 5 236 214 171 132 1270
Fragments arranged by size:
1270 236 214 171 132 5
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 144 966 2028
Size: 144 822 1062
Fragments arranged by size:
1062 822 144
BtsI GCAGTG_nn'
Cuts at: 0 1408 1935 2028
Size: 1408 527 93
Fragments arranged by size:
1408 527 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 268 2028
Size: 268 1760
EaeI y'GGCC_r
Cuts at: 0 2 89 200 244 452 674 1571 2028
Size: 2 87 111 44 208 222 897 457
Fragments arranged by size:
897 457 222 208 111 87 44 2
EagI C'GGCC_G
Cuts at: 0 200 244 2028
Size: 200 44 1784
Fragments arranged by size:
1784 200 44
EarI CTCTTCn'nnn_
Cuts at: 0 472 1340 1393 1960 2028
Size: 472 868 53 567 68
Fragments arranged by size:
868 567 472 68 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 508 519 2028
Size: 136 372 11 1509
Fragments arranged by size:
1509 372 136 11
EcoICRI GAG'CTC
Cuts at: 0 1953 2028
Size: 1953 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 399 762 1078 1319 2028
Size: 133 266 363 316 241 709
Fragments arranged by size:
709 363 316 266 241 133
EcoO109I rG'GnC_Cy
Cuts at: 0 178 1194 1461 1904 2010 2011 2028
Size: 178 1016 267 443 106 1 17
Fragments arranged by size:
1016 443 267 178 106 17 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 120 2028
Size: 120 1908
FspI TGC'GCA
Cuts at: 0 1663 2028
Size: 1663 365
FspAI rTGC'GCAy
Cuts at: 0 1663 2028
Size: 1663 365
HaeII r_GCGC'y
Cuts at: 0 42 68 173 1769 2028
Size: 42 26 105 1596 259
Fragments arranged by size:
1596 259 105 42 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 1728 1760 2008 2028
Size: 1351 32 345 32 248 20
Fragments arranged by size:
1351 345 248 32 32 20
HincII GTy'rAC
Cuts at: 0 723 1374 1944 2028
Size: 723 651 570 84
Fragments arranged by size:
723 651 570 84
KasI G'GCGC_C
Cuts at: 0 64 169 1765 2028
Size: 64 105 1596 263
Fragments arranged by size:
1596 263 105 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 2028
Size: 254 1774
MscI TGG'CCA
Cuts at: 0 91 454 1573 2028
Size: 91 363 1119 455
Fragments arranged by size:
1119 455 363 91
MslI CAynn'nnrTG
Cuts at: 0 1137 1831 2028
Size: 1137 694 197
Fragments arranged by size:
1137 694 197
MspA1I CmG'CkG
Cuts at: 0 7 243 1030 1515 1803 1845 1880 1992
Size: 7 236 787 485 288 42 35 112
Cuts at: 1992 2028
Size: 36
Fragments arranged by size:
787 485 288 236 112 42 36 35
7
NaeI GCC'GGC
Cuts at: 0 534 2028
Size: 534 1494
NarI GG'CG_CC
Cuts at: 0 65 170 1766 2028
Size: 65 105 1596 262
Fragments arranged by size:
1596 262 105 65
NcoI C'CATG_G
Cuts at: 0 626 2028
Size: 626 1402
NgoMIV G'CCGG_C
Cuts at: 0 532 2028
Size: 532 1496
NotI GC'GGCC_GC
Cuts at: 0 244 2028
Size: 244 1784
NspI r_CATG'y
Cuts at: 0 1428 1560 1836 2028
Size: 1428 132 276 192
Fragments arranged by size:
1428 276 192 132
PasI CC'CwG_GG
Cuts at: 0 23 2028
Size: 23 2005
PflMI CCAn_nnn'nTGG
Cuts at: 0 356 1815 2028
Size: 356 1459 213
Fragments arranged by size:
1459 356 213
PfoI T'CCnGG_A
Cuts at: 0 1296 2028
Size: 1296 732
PmlI CAC'GTG
Cuts at: 0 1331 2028
Size: 1331 697
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1147 1179 2028
Size: 1147 32 849
Fragments arranged by size:
1147 849 32
PpuMI rG'GwC_Cy
Cuts at: 0 178 1461 2028
Size: 178 1283 567
Fragments arranged by size:
1283 567 178
PshAI GACnn'nnGTC
Cuts at: 0 967 2028
Size: 967 1061
PspOMI G'GGCC_C
Cuts at: 0 1904 2010 2028
Size: 1904 106 18
Fragments arranged by size:
1904 106 18
PspXI vC'TCGA_Gb
Cuts at: 0 340 2028
Size: 340 1688
PstI C_TGCA'G
Cuts at: 0 701 1679 2028
Size: 701 978 349
Fragments arranged by size:
978 701 349
PvuI CG_AT'CG
Cuts at: 0 1763 2028
Size: 1763 265
PvuII CAG'CTG
Cuts at: 0 1515 1803 1845 1992 2028
Size: 1515 288 42 147 36
Fragments arranged by size:
1515 288 147 42 36
SacI G_AGCT'C
Cuts at: 0 1955 2028
Size: 1955 73
SacII CC_GC'GG
Cuts at: 0 8 244 2028
Size: 8 236 1784
Fragments arranged by size:
1784 236 8
SalI G'TCGA_C
Cuts at: 0 721 1372 1942 2028
Size: 721 651 570 86
Fragments arranged by size:
721 651 570 86
SapI GCTCTTCn'nnn_
Cuts at: 0 1340 1393 1960 2028
Size: 1340 53 567 68
Fragments arranged by size:
1340 567 68 53
ScaI AGT'ACT
Cuts at: 0 1564 1633 2028
Size: 1564 69 395
Fragments arranged by size:
1564 395 69
SfcI C'TryA_G
Cuts at: 0 279 697 1675 2028
Size: 279 418 978 353
Fragments arranged by size:
978 418 353 279
SfoI GGC'GCC
Cuts at: 0 66 171 1767 2028
Size: 66 105 1596 261
Fragments arranged by size:
1596 261 105 66
SgrAI Cr'CCGG_yG
Cuts at: 0 249 2028
Size: 249 1779
SmlI C'TyrA_G
Cuts at: 0 340 2028
Size: 340 1688
SphI G_CATG'C
Cuts at: 0 1428 1560 2028
Size: 1428 132 468
Fragments arranged by size:
1428 468 132
StyI C'CwwG_G
Cuts at: 0 234 626 650 2028
Size: 234 392 24 1378
Fragments arranged by size:
1378 392 234 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 1236 1509 1516 1930 2028
Size: 325 911 273 7 414 98
Fragments arranged by size:
911 414 325 273 98 7
TatI w'GTAC_w
Cuts at: 0 797 1562 1631 2028
Size: 797 765 69 397
Fragments arranged by size:
797 765 397 69
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1242 2028
Size: 1242 786
XhoI C'TCGA_G
Cuts at: 0 340 2028
Size: 340 1688
XmnI GAAnn'nnTTC
Cuts at: 0 364 1087 1582 2028
Size: 364 723 495 446
Fragments arranged by size:
723 495 446 364
ZraI GAC'GTC
Cuts at: 0 1620 2028
Size: 1620 408
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AlfI AloI
AlwNI ApaI ApoI AvaI BanI BanII BbeI BbsI
BcgI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PasI PflMI PfoI PmlI PpiI
PpuMI PshAI PspOMI PspXI PstI PvuI PvuII SacI
SacII SalI SapI ScaI SfcI SfoI SgrAI SmlI
SphI StyI TaqII TatI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AhdI AleI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BlpI BmtI BpuEI BsaI BsiWI BsmI BsrDI
BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI DraI
DraIII EcoNI EcoRI EcoRV FseI HindIII HpaI KpnI
MfeI MluI NdeI NheI NruI NsiI PacI PciI
PmeI PsiI PsrI RsrII SanDI SbfI SexAI SfiI
SgfI SmaI SnaBI SpeI SrfI SspI StuI SwaI
TstI Tth111I XbaI XmaI