(Circular) (Six-base) MAPSORT of: pFN21AB7191.seq Check: 6857 from: 1 to: 6328 pFN21AB7191 6328 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 2355 4455 4828 1563 Size: 792 2100 373 3063 Fragments arranged by size: 3063 2100 792 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4391 5775 6043 1632 Size: 2759 1384 268 1917 Fragments arranged by size: 2759 1917 1384 268 AfeI AGC'GCT Cuts at: 2977 2977 Size: 6328 AflII C'TTAA_G Cuts at: 820 1017 3067 820 Size: 197 2050 4081 Fragments arranged by size: 4081 2050 197 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 5159 5159 Size: 6328 AhdI GACnn_n'nnGTC Cuts at: 2566 5057 2566 Size: 2491 3837 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6328 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3017 1184 Size: 1833 4495 AlwNI CAG_nnn'CTG Cuts at: 2303 2651 4071 5643 2303 Size: 348 1420 1572 2988 Fragments arranged by size: 2988 1572 1420 348 ApaLI G'TGCA_C Cuts at: 4385 5541 4385 Size: 1156 5172 ApoI r'AATT_y Cuts at: 1493 2220 2410 3637 3863 4015 6124 1493 Size: 727 190 1227 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 727 226 190 152 AseI AT'TA_AT Cuts at: 160 1032 2699 4882 160 Size: 872 1667 2183 1606 Fragments arranged by size: 2183 1667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 3653 1870 Size: 73 1710 4545 Fragments arranged by size: 4545 1710 73 BamHI G'GATC_C Cuts at: 3658 4025 3658 Size: 367 5961 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3649 5104 618 Size: 325 217 627 1862 1455 1842 Fragments arranged by size: 1862 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3572 3647 1391 Size: 2181 75 4072 Fragments arranged by size: 4072 2181 75 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2521 928 Size: 971 622 4735 Fragments arranged by size: 4735 971 622 BbvCI CC'TCA_GC Cuts at: 3554 3554 Size: 6328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4519 4553 1903 Size: 34 2582 34 3678 Fragments arranged by size: 3678 2582 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BfrBI ATG'CAT Cuts at: 2737 2737 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4939 136 Size: 107 122 71 631 605 3267 1525 Fragments arranged by size: 3267 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2255 3561 6323 2255 Size: 1306 2762 2260 Fragments arranged by size: 2762 2260 1306 BlpI GC'TnA_GC Cuts at: 3571 3745 3571 Size: 174 6154 Bme1580I G_kGCm'C Cuts at: 1790 4389 5545 1790 Size: 2599 1156 2573 Fragments arranged by size: 2599 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5017 442 Size: 416 53 83 399 24 2642 958 1753 Fragments arranged by size: 2642 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3472 3472 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2280 3586 4988 1239 Size: 1041 1306 1402 2579 Fragments arranged by size: 2579 1402 1306 1041 Bpu10I CC'TnA_GC Cuts at: 3554 3554 Size: 6328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2504 4453 5318 5616 2504 Size: 1949 865 298 3216 Fragments arranged by size: 3216 1949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2417 3397 4991 882 Size: 617 918 980 1594 2219 Fragments arranged by size: 2219 1594 980 918 617 BsaAI yAC'GTr Cuts at: 493 1204 4119 493 Size: 711 2915 2702 Fragments arranged by size: 2915 2702 711 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4761 5159 5433 5580 5991 4761 Size: 398 274 147 411 5098 Fragments arranged by size: 5098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6103 Fragments arranged by size: 6103 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 4971 BseYI C'CCAG_C Cuts at: 3347 5531 6027 3347 Size: 2184 496 3648 Fragments arranged by size: 3648 2184 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2601 3033 1163 Size: 573 865 432 4458 Fragments arranged by size: 4458 865 573 432 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 4539 5567 1540 Size: 275 186 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3572 4389 4474 5545 3572 Size: 817 85 1071 4355 Fragments arranged by size: 4355 1071 817 85 BsmI GAATG_Cn' Cuts at: 3839 3932 3839 Size: 93 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3572 3647 4389 4474 5545 1391 Size: 399 1782 75 742 85 1071 2174 Fragments arranged by size: 2174 1782 1071 742 399 85 75 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 2136 4217 5947 2136 Size: 2081 1730 2517 Fragments arranged by size: 2517 2081 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3683 844 Size: 2839 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4823 4997 65 Size: 1183 3575 174 1396 Fragments arranged by size: 3575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4972 5159 1700 Size: 110 3162 187 2869 Fragments arranged by size: 3162 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2155 96 Size: 2059 4269 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 2999 3218 4388 5400 2999 Size: 219 1170 1012 3927 Fragments arranged by size: 3927 1170 1012 219 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 5132 5132 Size: 6328 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstXI CCAn_nnnn'nTGG Cuts at: 3313 3388 3313 Size: 75 6253 BstYI r'GATC_y Cuts at: 1972 2255 3561 3658 4025 4410 4427 5879 5965 Size: 283 1306 97 367 385 17 1452 86 Cuts at: 5965 5977 6323 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1306 385 367 346 283 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4837 Fragments arranged by size: 4837 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 3844 4638 4658 3844 Size: 794 20 5514 Fragments arranged by size: 5514 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3145 3632 3145 Size: 487 5841 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2525 5335 809 Size: 507 1209 2810 1802 Fragments arranged by size: 2810 1802 1209 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2661 3775 4664 Size: 54 1425 50 275 18 831 1114 889 Cuts at: 4664 6293 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1114 889 831 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3775 1537 Size: 275 1963 4090 Fragments arranged by size: 4090 1963 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4258 1353 Size: 2905 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4915 5289 5435 387 Size: 1064 138 3326 374 146 1280 Fragments arranged by size: 3326 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3570 3570 Size: 6328 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2280 3586 4391 4988 5775 6043 1239 Size: 393 648 1306 805 597 787 268 1524 Fragments arranged by size: 1524 1306 805 787 648 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2560 3490 2560 Size: 930 5398 EcoRI G'AATT_C Cuts at: 3637 4015 3637 Size: 378 5950 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2265 2836 2265 Size: 571 5757 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 2979 5475 1678 Size: 1301 2496 2531 Fragments arranged by size: 2531 2496 1301 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2552 2584 4969 5001 5043 Size: 32 135 32 980 32 2385 32 42 Cuts at: 5043 5075 1373 Size: 32 2626 Fragments arranged by size: 2626 2385 980 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2041 3672 3918 669 Size: 988 281 103 1631 246 3079 Fragments arranged by size: 3079 1631 988 281 246 103 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3468 3927 3468 Size: 459 5869 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 3495 3501 5442 5626 1305 Size: 1104 1086 6 1941 184 2007 Fragments arranged by size: 2007 1941 1104 1086 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2663 10 Size: 54 1425 343 831 3675 Fragments arranged by size: 3675 1425 831 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2577 2867 4286 4645 4804 518 Size: 648 1411 290 1419 359 159 2042 Fragments arranged by size: 2042 1419 1411 648 359 290 159 MspA1I CmG'CkG Cuts at: 1606 3017 3744 4421 5569 5814 1606 Size: 1411 727 677 1148 245 2120 Fragments arranged by size: 2120 1411 1148 727 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4837 Fragments arranged by size: 4837 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NsiI A_TGCA'T Cuts at: 2739 2739 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 2737 3378 3686 5231 1132 Size: 97 1508 641 308 1545 2229 Fragments arranged by size: 2229 1545 1508 641 308 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6328 PfoI T'CCnGG_A Cuts at: 2556 2556 Size: 6328 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2560 2560 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 3898 3898 Size: 6328 PstI C_TGCA'G Cuts at: 830 2986 3680 830 Size: 2156 694 3478 Fragments arranged by size: 3478 2156 694 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 PvuII CAG'CTG Cuts at: 3017 3017 Size: 6328 SacI G_AGCT'C Cuts at: 3572 3572 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SanDI GG'GwC_CC Cuts at: 2560 2560 Size: 6328 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SfcI C'TryA_G Cuts at: 826 1045 2982 3676 4811 5492 5683 826 Size: 219 1937 694 1135 681 191 1471 Fragments arranged by size: 1937 1471 1135 694 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6328 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2483 3067 4432 5333 5595 820 Size: 197 926 540 584 1365 901 262 1553 Fragments arranged by size: 1553 1365 926 901 584 540 262 197 SnaBI TAC'GTA Cuts at: 493 4119 493 Size: 3626 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6328 SphI G_CATG'C Cuts at: 1132 3378 3686 1132 Size: 2246 308 3774 Fragments arranged by size: 3774 2246 308 SspI AAT'ATT Cuts at: 5 52 2776 3508 4252 5 Size: 47 2724 732 744 2081 Fragments arranged by size: 2724 2081 744 732 47 StuI AGG'CCT Cuts at: 1517 3387 1517 Size: 1870 4458 StyI C'CwwG_G Cuts at: 513 1065 2004 3577 3767 513 Size: 552 939 1573 190 3074 Fragments arranged by size: 3074 1573 939 552 190 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2634 2906 3437 4355 4372 4525 4710 Size: 1255 1054 272 531 918 17 153 185 Cuts at: 4710 5155 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1054 918 531 445 272 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2155 3472 4574 Size: 274 80 33 51 1443 178 1317 1102 Cuts at: 4574 6286 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1317 1102 274 178 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2303 2335 6288 6320 2303 Size: 32 3953 32 2311 Fragments arranged by size: 3953 2311 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3273 1316 Size: 1957 4371 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 2224 2248 4457 2224 Size: 24 2209 4095 Fragments arranged by size: 4095 2209 24 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI