Restriction map for
pFN21AB7188
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# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI AocI Aor51HI ApaI ApoI
AscI AseI AsnI Asp718I AspEI
AspI AviII BamHI BbrPI BbvCI
BciVI BclI BfrI BlpI Bpu1102I
BsaBI BsaI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstAPI BstBI
BstXI BstZ17I Bsu36I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
SacI SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tth111I Van91I
XbaI XhoI
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# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 1253 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 747 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 165 CCTAGG C^CTAGG
BglI 484 GCCCGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 194 AGATCT A^GATCT
BlnI 165 CCTAGG C^CTAGG
BsaWI 274 ACCGGA [AT]^CCGG[AT]
BsiEI 459 CGACCG CG[GA][CT]^CG
BspMI 809 ACCTGC ACCTGC(4/8)
BssHII 1177 GCGCGC G^CGCGC
BssSI 1083 CTCGTG CACGAG(-5/-1)
BstEII 369 GGTGACC G^GT[ACGT]ACC
BstPI 369 GGTGACC G^GT[ACGT]ACC
BstYI 194 AGATCT [GA]^GATC[CT]
DraIII 997 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 93 GAAGAG CTCTTC(1/4)
EcoO65I 369 GGTGACC G^GT[ACGT]ACC
Ksp632I 93 GAAGAG CTCTTC(1/4)
MflI 194 AGATCT [GA]^GATC[CT]
MslI 330 CATCTCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 476 GCATGC [GA]CATG^[CT]
PpuMI 647 AGGACCT [GA]G^G[AT]CC[CT]
SfaNI 1292 GCATC GCATC(5/9)
SfcI 702 CTACAG C^T[GA][CT]AG
SphI 476 GCATGC GCATG^C
Tsp509I 235 AATT ^AATT
XcmI 608 CCACGGTGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 194 AGATCT [GA]^GATC[CT]
XmnI 747 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Alw44I 613 GTGCAC G^TGCAC
994 GTGCAC
ApaLI 613 GTGCAC G^TGCAC
994 GTGCAC
BbsI 159 GAAGAC GAAGAC(2/6)
751 GTCTTC
BfaI 28 CTAG C^TAG
166 CTAG
BpuAI 159 GAAGAC GAAGAC(2/6)
751 GTCTTC
BsaAI 757 TACGTG [CT]AC^GT[GA]
946 TACGTG
BsmBI 36 CGTCTC CGTCTC(1/5)
766 GAGACG
BstF5I 1293 CATCC GGATG(2/0)
261 GGATG
CpoI 72 CGGACCG CG^G[AT]CCG
1184 CGGACCG
DpnII 195 GATC ^GATC
762 GATC
EcoNI 618 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1008 CCTAAGCGAGG
EcoT14I 165 CCTAGG C^C[AT][AT]GG
530 CCAAGG
FokI 1293 CATCC GGATG(9/13)
261 GGATG
MaeI 28 CTAG C^TAG
166 CTAG
MboI 195 GATC ^GATC
762 GATC
MseI 218 TTAA T^TAA
257 TTAA
NdeII 195 GATC ^GATC
762 GATC
NlaIII 477 CATG CATG^
1368 CATG
PvuII 1110 CAGCTG CAG^CTG
1309 CAGCTG
RsrII 72 CGGACCG CG^G[AT]CCG
1184 CGGACCG
Sau3AI 195 GATC ^GATC
762 GATC
StyI 165 CCTAGG C^C[AT][AT]GG
530 CCAAGG
Tru9I 218 TTAA T^TAA
257 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcgI BcnI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssKI BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI KspI MaeII
MaeIII MboII MluNI MnlI MscI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIV
PleI RsaI SacII Sau96I ScrFI
SfoI SmaI TaqI TseI Tsp45I
TspRI TthHB8I XmaCI XmaI