(Circular) (Six-base) MAPSORT of: pFN21AB7188.seq Check: 9113 from: 1 to: 6091 pFN21AB7188 6091 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2823 2823 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3901 278 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4154 5538 5806 1632 Size: 2522 1384 268 1917 Fragments arranged by size: 2522 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 4990 5967 1203 Size: 3787 977 1327 Fragments arranged by size: 3787 1327 977 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6091 AlwNI CAG_nnn'CTG Cuts at: 3263 3834 5406 3263 Size: 571 1572 3948 Fragments arranged by size: 3948 1572 571 ApaLI G'TGCA_C Cuts at: 2618 2999 4148 5304 2618 Size: 381 1149 1156 3405 Fragments arranged by size: 3405 1156 1149 381 ApoI r'AATT_y Cuts at: 1493 3400 3626 3778 5887 1493 Size: 1907 226 152 2109 1697 Fragments arranged by size: 2109 1907 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2602 2663 2858 2869 2875 2907 Size: 73 119 540 61 195 11 6 32 Cuts at: 2907 2935 3416 1870 Size: 28 481 4545 Fragments arranged by size: 4545 540 481 195 119 73 61 32 28 11 6 AvrII C'CTAG_G Cuts at: 2170 2170 Size: 6091 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2106 2174 2636 2744 2827 Size: 325 217 627 319 68 462 108 83 Cuts at: 2827 3032 3059 3175 3332 3412 4867 618 Size: 205 27 116 157 80 1455 1842 Fragments arranged by size: 1842 1455 627 462 325 319 217 205 157 116 108 83 80 68 27 BanII G_rGCy'C Cuts at: 1391 2609 2663 2859 2876 3410 1391 Size: 1218 54 196 17 534 4072 Fragments arranged by size: 4072 1218 534 196 54 17 BbeI G_GCGC'C Cuts at: 2178 2748 2831 3036 3179 2178 Size: 570 83 205 143 5090 Fragments arranged by size: 5090 570 205 143 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2749 928 Size: 971 272 578 4270 Fragments arranged by size: 4270 971 578 272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2008 2021 2042 2074 2108 2553 Size: 34 50 21 13 21 32 34 445 Cuts at: 2553 2587 4282 4316 1903 Size: 34 1695 34 3678 Fragments arranged by size: 3678 1695 445 50 34 34 34 34 32 21 21 13 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2495 4702 136 Size: 107 122 71 631 605 823 2207 1525 Fragments arranged by size: 2207 1525 823 631 605 122 107 71 BglII A'GATC_T Cuts at: 2199 6086 2199 Size: 3887 2204 BlpI GC'TnA_GC Cuts at: 3508 3508 Size: 6091 Bme1580I G_kGCm'C Cuts at: 1790 2622 3003 3062 4152 5308 1790 Size: 832 381 59 1090 1156 2573 Fragments arranged by size: 2573 1156 1090 832 381 59 BmgBI CAC'GTC Cuts at: 1318 2454 1318 Size: 1136 4955 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2527 2909 3822 Size: 416 53 83 399 24 1110 382 913 Cuts at: 3822 4780 442 Size: 958 1753 Fragments arranged by size: 1753 1110 958 913 416 399 382 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2884 2884 Size: 6091 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2188 2645 3240 4751 1239 Size: 949 457 595 1511 2579 Fragments arranged by size: 2579 1511 949 595 457 Bpu10I CC'TnA_GC Cuts at: 2657 3014 2657 Size: 357 5734 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2764 2953 3882 493 Size: 711 1560 189 929 2702 Fragments arranged by size: 2702 1560 929 711 189 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2175 2745 2828 2864 Size: 53 83 186 936 642 570 83 36 Cuts at: 2864 3033 3176 3898 4280 4915 275 Size: 169 143 722 382 635 1451 Fragments arranged by size: 1451 936 722 642 635 570 382 186 169 143 83 83 53 36 BsaWI w'CCGG_w Cuts at: 2279 4524 4922 5196 5343 5754 2279 Size: 2245 398 274 147 411 2616 Fragments arranged by size: 2616 2245 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5866 Fragments arranged by size: 5866 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2035 2116 2426 3035 3122 1186 Size: 849 81 310 609 87 4155 Fragments arranged by size: 4155 849 609 310 87 81 BseYI C'CCAG_C Cuts at: 2385 3117 3367 5294 5790 2385 Size: 732 250 1927 496 2686 Fragments arranged by size: 2686 1927 732 496 250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2405 3153 3344 1163 Size: 573 669 748 191 3910 Fragments arranged by size: 3910 748 669 573 191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2467 3541 4302 5330 1540 Size: 275 186 466 1074 761 1028 2301 Fragments arranged by size: 2301 1074 1028 761 466 275 186 BsiHKAI G_wGCw'C Cuts at: 2622 3003 3114 4152 4237 5308 2622 Size: 381 111 1038 85 1071 3405 Fragments arranged by size: 3405 1071 1038 381 111 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 BsmBI CGTCTCn'nnnn_ Cuts at: 2047 2765 2047 Size: 718 5373 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2609 2622 2663 2859 2876 3003 3062 Size: 399 819 13 41 196 17 127 59 Cuts at: 3062 3114 3410 4152 4237 5308 1391 Size: 52 296 742 85 1071 2174 Fragments arranged by size: 2174 1071 819 742 399 296 196 127 85 59 52 41 17 13 BspEI T'CCGG_A Cuts at: 5754 5754 Size: 6091 BspHI T'CATG_A Cuts at: 3980 5710 3980 Size: 1730 4361 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2823 3446 844 Size: 1979 623 3489 Fragments arranged by size: 3489 1979 623 BsrBI CCG'CTC Cuts at: 1394 1823 2285 3046 3254 3978 1394 Size: 429 462 761 208 724 3507 Fragments arranged by size: 3507 761 724 462 429 208 BsrDI GCAATG_nn' Cuts at: 65 1248 4586 4760 65 Size: 1183 3338 174 1396 Fragments arranged by size: 3338 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2507 2697 2979 4735 4922 1700 Size: 110 697 190 282 1756 187 2869 Fragments arranged by size: 2869 1756 697 282 190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 3182 1925 Size: 1257 4834 BssSI C'ACGA_G Cuts at: 3088 4151 5163 3088 Size: 1063 1012 4016 Fragments arranged by size: 4016 1063 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6091 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 2374 1184 Size: 1190 4901 BstYI r'GATC_y Cuts at: 1972 2199 3421 3788 4173 4190 5642 5728 5740 Size: 227 1222 367 385 17 1452 86 12 Cuts at: 5740 6086 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1222 385 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2613 3027 3193 3220 3291 513 Size: 552 1548 414 166 27 71 3313 Fragments arranged by size: 3313 1548 552 414 166 71 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5352 Fragments arranged by size: 5352 683 56 BtsI GCAGTG_nn' Cuts at: 2445 2548 3607 4401 4421 2445 Size: 103 1059 794 20 4115 Fragments arranged by size: 4115 1059 794 103 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DraIII CAC_nnn'GTG Cuts at: 3007 3007 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2331 2354 Size: 54 1425 50 275 18 327 174 23 Cuts at: 2354 2398 2580 2610 2844 2958 3538 4427 6056 Size: 44 182 30 234 114 580 889 1629 Cuts at: 6056 8 Size: 43 Fragments arranged by size: 1629 1425 889 580 327 275 234 182 174 114 54 50 44 43 30 23 18 EagI C'GGCC_G Cuts at: 1537 1812 3538 1537 Size: 275 1726 4090 Fragments arranged by size: 4090 1726 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 4021 1353 Size: 740 1928 3423 Fragments arranged by size: 3423 1928 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2699 3134 4678 5052 5198 387 Size: 1064 138 1110 435 1544 374 146 1280 Fragments arranged by size: 1544 1280 1110 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2188 2645 3240 4154 4751 5538 5806 Size: 393 556 457 595 914 597 787 268 Cuts at: 5806 1239 Size: 1524 Fragments arranged by size: 1524 914 787 597 595 556 457 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2627 3017 2627 Size: 390 5701 EcoO109I rG'GnC_Cy Cuts at: 2653 2903 3355 2653 Size: 250 452 5389 Fragments arranged by size: 5389 452 250 EcoRI G'AATT_C Cuts at: 3400 3778 3400 Size: 378 5713 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2773 2773 Size: 6091 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 2178 2748 2794 2831 2915 3036 3179 5238 Size: 500 570 46 37 84 121 143 2059 Cuts at: 5238 1678 Size: 2531 Fragments arranged by size: 2531 2059 570 500 143 121 84 46 37 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4732 4764 4806 4838 1373 Size: 32 135 32 3160 32 42 32 2626 Fragments arranged by size: 3160 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KasI G'GCGC_C Cuts at: 2174 2744 2827 3032 3175 2174 Size: 570 83 205 143 5090 Fragments arranged by size: 5090 570 205 143 83 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5205 5389 1305 Size: 3900 184 2007 Fragments arranged by size: 3900 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2333 2356 2400 10 Size: 54 1425 343 501 23 44 3701 Fragments arranged by size: 3701 1425 501 343 54 44 23 MslI CAynn'nnrTG Cuts at: 518 1166 2339 4049 4408 4567 518 Size: 648 1173 1710 359 159 2042 Fragments arranged by size: 2042 1710 1173 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2181 2996 3029 3117 3195 3207 3222 3293 Size: 575 815 33 88 78 12 15 71 Cuts at: 3293 3316 3367 3507 4184 5332 5577 1606 Size: 23 51 140 677 1148 245 2120 Fragments arranged by size: 2120 1148 815 677 575 245 140 88 78 71 51 33 23 15 12 NaeI GCC'GGC Cuts at: 2509 2699 2981 2509 Size: 190 282 5619 Fragments arranged by size: 5619 282 190 NarI GG'CG_CC Cuts at: 2175 2745 2828 3033 3176 2175 Size: 570 83 205 143 5090 Fragments arranged by size: 5090 570 205 143 83 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NgoMIV G'CCGG_C Cuts at: 2507 2697 2979 2507 Size: 190 282 5619 Fragments arranged by size: 5619 282 190 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2485 3449 4994 1132 Size: 97 1256 964 1545 2229 Fragments arranged by size: 2229 1545 1256 964 97 PasI CC'CwG_GG Cuts at: 1348 3055 1348 Size: 1707 4384 PciI A'CATG_T Cuts at: 4990 4990 Size: 6091 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6091 PfoI T'CCnGG_A Cuts at: 2727 3342 2727 Size: 615 5476 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2653 2653 Size: 6091 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6091 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PstI C_TGCA'G Cuts at: 830 3443 830 Size: 2613 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 3117 3316 3117 Size: 199 5892 RsrII CG'GwC_CG Cuts at: 2078 3190 2078 Size: 1112 4979 SacII CC_GC'GG Cuts at: 3030 3196 3223 3294 3030 Size: 166 27 71 5827 Fragments arranged by size: 5827 166 71 27 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 4339 6051 4339 Size: 1712 4379 SfcI C'TryA_G Cuts at: 826 1045 2707 3439 4574 5255 5446 826 Size: 219 1662 732 1135 681 191 1471 Fragments arranged by size: 1662 1471 1135 732 681 219 191 SfoI GGC'GCC Cuts at: 2176 2746 2829 3034 3177 2176 Size: 570 83 205 143 5090 Fragments arranged by size: 5090 570 205 143 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 2604 2665 2937 3418 1872 Size: 732 61 272 481 4545 Fragments arranged by size: 4545 732 481 272 61 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2485 3449 1132 Size: 1353 964 3774 Fragments arranged by size: 3774 1353 964 SrfI GCCC'GGGC Cuts at: 2937 2937 Size: 6091 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6091 StyI C'CwwG_G Cuts at: 513 1065 2170 2535 3530 513 Size: 552 1105 365 995 3074 Fragments arranged by size: 3074 1105 995 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2393 2509 4118 4135 4288 4473 4918 Size: 1255 813 116 1609 17 153 185 445 Cuts at: 4918 325 Size: 1498 Fragments arranged by size: 1609 1498 1255 813 445 185 153 116 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2523 4337 6049 Size: 274 80 33 51 1443 546 1814 1712 Cuts at: 6049 96 Size: 138 Fragments arranged by size: 1814 1712 1443 546 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6051 6083 6051 Size: 32 6059 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2620 2011 Size: 609 5482 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 2602 2663 2935 3416 1870 Size: 732 61 272 481 4545 Fragments arranged by size: 4545 732 481 272 61 XmnI GAAnn'nnTTC Cuts at: 2756 4220 2756 Size: 1464 4627 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3899 276 Size: 53 83 186 936 2365 2468 Fragments arranged by size: 2468 2365 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SanDI SapI SexAI SfiI SwaI