Restriction map for
pFN21AB7181
(Circular) (Six-base) MAPSORT of: pFN21AB7181.seq Check: 6909 from: 1 to: 5770
pFN21AB7181 5770 bp
With 182 enzymes: SgfI *
February 27, 2009 14:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2025 3071 2025
Size: 1046 4724
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3580 278
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2829 3113 1937
Size: 892 284 4594
Fragments arranged by size:
4594 892 284
Acc65I G'GTAC_C
Cuts at: 3091 3091
Size: 5770
AclI AA'CG_TT
Cuts at: 1563 3897 4270 1563
Size: 2334 373 3063
Fragments arranged by size:
3063 2334 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3833 5217 5485 1632
Size: 2201 1384 268 1917
Fragments arranged by size:
2201 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5573
AflIII A'CryG_T
Cuts at: 1203 4669 5646 1203
Size: 3466 977 1327
Fragments arranged by size:
3466 1327 977
AgeI A'CCGG_T
Cuts at: 4601 4601
Size: 5770
AhdI GACnn_n'nnGTC
Cuts at: 4499 4499
Size: 5770
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5770
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5770
AlwNI CAG_nnn'CTG
Cuts at: 2131 3513 5085 2131
Size: 1382 1572 2816
Fragments arranged by size:
2816 1572 1382
ApaI G_GGCC'C
Cuts at: 2313 2313
Size: 5770
ApaLI G'TGCA_C
Cuts at: 3056 3827 4983 3056
Size: 771 1156 3843
Fragments arranged by size:
3843 1156 771
ApoI r'AATT_y
Cuts at: 1493 3079 3305 3457 5566 1493
Size: 1586 226 152 2109 1697
Fragments arranged by size:
2109 1697 1586 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4324 160
Size: 872 3292 1606
Fragments arranged by size:
3292 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
AvaI C'yCGr_G
Cuts at: 1870 1943 2021 2760 3095 1870
Size: 73 78 739 335 4545
Fragments arranged by size:
4545 739 335 78 73
BamHI G'GATC_C
Cuts at: 3100 3467 3100
Size: 367 5403
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2051 2405 3091 4546 618
Size: 325 217 627 264 354 686 1455 1842
Fragments arranged by size:
1842 1455 686 627 354 325 264 217
BanII G_rGCy'C
Cuts at: 1391 2013 2123 2313 2862 3089 1391
Size: 622 110 190 549 227 4072
Fragments arranged by size:
4072 622 549 227 190 110
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2663 928
Size: 971 764 4035
Fragments arranged by size:
4035 971 764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2173 2207 3961 3995 1903
Size: 34 236 34 1754 34 3678
Fragments arranged by size:
3678 1754 236 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3662 4872 3662
Size: 1210 4560
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5770
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2372 4381 136
Size: 107 122 71 631 605 700 2009 1525
Fragments arranged by size:
2009 1525 700 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5765 5765
Size: 5770
BlpI GC'TnA_GC
Cuts at: 3187 3187
Size: 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2313 2408 3060 3831 4987 1790
Size: 523 95 652 771 1156 2573
Fragments arranged by size:
2573 1156 771 652 523 95
BmgBI CAC'GTC
Cuts at: 1318 3055 1318
Size: 1737 4033
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3501 4459 442
Size: 416 53 83 399 24 2084 958 1753
Fragments arranged by size:
2084 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5770
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2425 2425
Size: 5770
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4430 1239
Size: 3191 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2690 3895 4760 5058 2690
Size: 1205 865 298 3402
Fragments arranged by size:
3402 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2768 4433 882
Size: 617 1269 1665 2219
Fragments arranged by size:
2219 1665 1269 617
BsaAI yAC'GTr
Cuts at: 493 1204 3561 493
Size: 711 2357 2702
Fragments arranged by size:
2702 2357 711
BsaBI GATnn'nnATC
Cuts at: 5764 5764
Size: 5770
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3577 3959 4594 275
Size: 53 83 186 936 2044 382 635 1451
Fragments arranged by size:
2044 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2895 4203 4601 4875 5022 5433 2895
Size: 1308 398 274 147 411 3232
Fragments arranged by size:
3232 1308 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2825 2855 1178
Size: 30 165 30 1422 30 4093
Fragments arranged by size:
4093 1422 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2021 1186
Size: 835 4935
BseYI C'CCAG_C
Cuts at: 2204 2227 2766 4973 5469 2204
Size: 23 539 2207 496 2505
Fragments arranged by size:
2505 2207 539 496 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2260 1163
Size: 573 524 4673
Fragments arranged by size:
4673 573 524
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2367 3220 3981 5009 1540
Size: 275 186 366 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 366 275 186
BsiHKAI G_wGCw'C
Cuts at: 3060 3831 3916 4987 3060
Size: 771 85 1071 3843
Fragments arranged by size:
3843 1071 771 85
BsmI GAATG_Cn'
Cuts at: 3281 3374 3281
Size: 93 5677
BsmBI CGTCTCn'nnnn_
Cuts at: 2864 2864
Size: 5770
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2013 2123 2313 2408 2862 3060 3089
Size: 399 223 110 190 95 454 198 29
Cuts at: 3089 3831 3916 4987 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 454 399 223 198 190
110 95 85 29
BspEI T'CCGG_A
Cuts at: 5433 5433
Size: 5770
BspHI T'CATG_A
Cuts at: 3659 5389 3659
Size: 1730 4040
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2025 2669 3071 3125 844
Size: 1181 644 402 54 3489
Fragments arranged by size:
3489 1181 644 402 54
BsrBI CCG'CTC
Cuts at: 1394 1823 2391 3657 1394
Size: 429 568 1266 3507
Fragments arranged by size:
3507 1266 568 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2911 4265 4439 65
Size: 1183 1663 1354 174 1396
Fragments arranged by size:
1663 1396 1354 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2366 4414 4601 1700
Size: 110 556 2048 187 2869
Fragments arranged by size:
2869 2048 556 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5770
BssHII G'CGCG_C
Cuts at: 1925 2528 2549 1925
Size: 603 21 5146
Fragments arranged by size:
5146 603 21
BssSI C'ACGA_G
Cuts at: 2965 3830 4842 2965
Size: 865 1012 3893
Fragments arranged by size:
3893 1012 865
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5770
BstBI TT'CG_AA
Cuts at: 4574 4574
Size: 5770
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5770
BstXI CCAn_nnnn'nTGG
Cuts at: 2082 2082
Size: 5770
BstYI r'GATC_y
Cuts at: 1972 3100 3467 3852 3869 5321 5407 5419 5765
Size: 1128 367 385 17 1452 86 12 346
Cuts at: 5765 1972
Size: 1977
Fragments arranged by size:
1977 1452 1128 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2629 2811 2917 513
Size: 552 939 625 182 106 3366
Fragments arranged by size:
3366 939 625 552 182 106
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2341 487
Size: 683 56 1115 3916
Fragments arranged by size:
3916 1115 683 56
BtsI GCAGTG_nn'
Cuts at: 2995 3286 4080 4100 2995
Size: 291 794 20 4665
Fragments arranged by size:
4665 794 291 20
ClaI AT'CG_AT
Cuts at: 1554 2925 1554
Size: 1371 4399
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5735
DraI TTT'AAA
Cuts at: 3074 3074
Size: 5770
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4777 809
Size: 507 3461 1802
Fragments arranged by size:
3461 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2211 2298 2364
Size: 54 1425 50 275 18 381 87 66
Cuts at: 2364 2556 2950 3217 4106 5735 8
Size: 192 394 267 889 1629 43
Fragments arranged by size:
1629 1425 889 394 381 275 267 192
87 66 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2364 3217 1537
Size: 275 552 853 4090
Fragments arranged by size:
4090 853 552 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2804 3700 1353
Size: 1451 896 3423
Fragments arranged by size:
3423 1451 896
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4357 4731 4877 387
Size: 1064 138 2768 374 146 1280
Fragments arranged by size:
2768 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3833 4430 5217 5485 1239
Size: 393 2201 597 787 268 1524
Fragments arranged by size:
2201 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2454 2454
Size: 5770
EcoO109I rG'GnC_Cy
Cuts at: 2059 2821 2059
Size: 762 5008
EcoRI G'AATT_C
Cuts at: 3079 3457 3079
Size: 378 5392
FspI TGC'GCA
Cuts at: 4276 4276
Size: 5770
HaeII r_GCGC'y
Cuts at: 1678 2727 4917 1678
Size: 1049 2190 2531
Fragments arranged by size:
2531 2190 1049
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2537 2569 2711 2743 4411
Size: 32 135 32 965 32 142 32 1668
Cuts at: 4411 4443 4485 4517 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1668 965 142 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3114 3360 669
Size: 988 281 1176 246 3079
Fragments arranged by size:
3079 1176 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5770
HpaI GTT'AAC
Cuts at: 3360 3360
Size: 5770
KpnI G_GTAC'C
Cuts at: 3095 3095
Size: 5770
MfeI C'AATT_G
Cuts at: 3369 3369
Size: 5770
MluI A'CGCG_T
Cuts at: 5646 5646
Size: 5770
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4884 5068 1305
Size: 3579 184 2007
Fragments arranged by size:
3579 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2300 10
Size: 54 1425 343 468 3480
Fragments arranged by size:
3480 1425 468 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3728 4087 4246 518
Size: 648 2562 359 159 2042
Fragments arranged by size:
2562 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2268 2646 3027 3186 3863 5011 5256 1606
Size: 662 378 381 159 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 381 378 245 159
NaeI GCC'GGC
Cuts at: 2368 2368
Size: 5770
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4279
Fragments arranged by size:
4279 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5770
NgoMIV G'CCGG_C
Cuts at: 2366 2366
Size: 5770
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5770
NotI GC'GGCC_GC
Cuts at: 3217 3217
Size: 5770
NspI r_CATG'y
Cuts at: 1132 1229 2611 3128 4673 1132
Size: 97 1382 517 1545 2229
Fragments arranged by size:
2229 1545 1382 517 97
PasI CC'CwG_GG
Cuts at: 1348 2095 1348
Size: 747 5023
PciI A'CATG_T
Cuts at: 4669 4669
Size: 5770
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5770
PfoI T'CCnGG_A
Cuts at: 2235 2598 2235
Size: 363 5407
PmeI GTTT'AAAC
Cuts at: 3074 3074
Size: 5770
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3839 3871 5352 5384 3839
Size: 32 1481 32 4225
Fragments arranged by size:
4225 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2059 2821 2059
Size: 762 5008
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5770
PsiI TTA'TAA
Cuts at: 3340 3340
Size: 5770
PspOMI G'GGCC_C
Cuts at: 2309 2309
Size: 5770
PstI C_TGCA'G
Cuts at: 830 2480 3122 830
Size: 1650 642 3478
Fragments arranged by size:
3478 1650 642
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5770
PvuII CAG'CTG
Cuts at: 2646 3027 2646
Size: 381 5389
SalI G'TCGA_C
Cuts at: 1936 3112 1936
Size: 1176 4594
SapI GCTCTTCn'nnn_
Cuts at: 2804 2804
Size: 5770
SbfI CC_TGCA'GG
Cuts at: 3122 3122
Size: 5770
ScaI AGT'ACT
Cuts at: 2792 4018 5730 2792
Size: 1226 1712 2832
Fragments arranged by size:
2832 1712 1226
SexAI A'CCwGG_T
Cuts at: 2823 2823
Size: 5770
SfcI C'TryA_G
Cuts at: 826 1045 2476 3118 4253 4934 5125 826
Size: 219 1431 642 1135 681 191 1471
Fragments arranged by size:
1471 1431 1135 681 642 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2372 2372
Size: 5770
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5770
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5770
SmaI CCC'GGG
Cuts at: 1872 3097 1872
Size: 1225 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2705 3874 4775 5037 820
Size: 197 926 762 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 762 262 197
SnaBI TAC'GTA
Cuts at: 493 3561 493
Size: 3068 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5770
SphI G_CATG'C
Cuts at: 1132 3128 1132
Size: 1996 3774
SspI AAT'ATT
Cuts at: 5 52 3694 5
Size: 47 3642 2081
Fragments arranged by size:
3642 2081 47
StuI AGG'CCT
Cuts at: 1517 2154 1517
Size: 637 5133
StyI C'CwwG_G
Cuts at: 513 1065 2004 3209 513
Size: 552 939 1205 3074
Fragments arranged by size:
3074 1205 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2758 2824 3797 3814 3967 4152 4597
Size: 1255 1178 66 973 17 153 185 445
Cuts at: 4597 325
Size: 1498
Fragments arranged by size:
1498 1255 1178 973 445 185 153 66
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2106 2790 4016
Size: 274 80 33 51 1443 129 684 1226
Cuts at: 4016 5728 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1226 684 274 138 129 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2741 2773 5730 5762 2741
Size: 32 2957 32 2749
Fragments arranged by size:
2957 2749 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2825 1316
Size: 1509 4261
XbaI T'CTAG_A
Cuts at: 3106 3106
Size: 5770
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2152 2924 2152
Size: 772 4998
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5770
XmaI C'CCGG_G
Cuts at: 1870 3095 1870
Size: 1225 4545
XmnI GAAnn'nnTTC
Cuts at: 3899 3899
Size: 5770
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3578 276
Size: 53 83 186 936 2044 2468
Fragments arranged by size:
2468 2044 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI
FseI FspAI KasI NarI NruI NsiI PacI PmlI
PspXI PsrI RsrII SacI SacII SanDI SfoI SrfI
SwaI