(Circular) (Six-base) MAPSORT of: pFN21AB7180.seq Check: 5657 from: 1 to: 5965 pFN21AB7180 5965 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3230 3230 Size: 5965 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3308 1937 Size: 1371 4594 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2664 4028 5412 5680 1632 Size: 1032 1364 1384 268 1917 Fragments arranged by size: 1917 1384 1364 1032 268 AfeI AGC'GCT Cuts at: 2801 2801 Size: 5965 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 2264 4864 5841 1203 Size: 1061 2600 977 1327 Fragments arranged by size: 2600 1327 1061 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5965 AlwNI CAG_nnn'CTG Cuts at: 2199 2325 3708 5280 2199 Size: 126 1383 1572 2884 Fragments arranged by size: 2884 1572 1383 126 ApaI G_GGCC'C Cuts at: 2231 2509 3043 2231 Size: 278 534 5153 Fragments arranged by size: 5153 534 278 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 3274 3500 3652 5761 1493 Size: 1781 226 152 2109 1697 Fragments arranged by size: 2109 1781 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 2219 2230 3290 1870 Size: 73 276 11 1060 4545 Fragments arranged by size: 4545 1060 276 73 11 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2372 3049 3178 3286 4741 Size: 325 217 627 585 677 129 108 1455 Cuts at: 4741 618 Size: 1842 Fragments arranged by size: 1842 1455 677 627 585 325 217 129 108 BanII G_rGCy'C Cuts at: 1391 2231 2285 2509 3043 3084 3284 1391 Size: 840 54 224 534 41 200 4072 Fragments arranged by size: 4072 840 534 224 200 54 41 BbeI G_GCGC'C Cuts at: 3182 3182 Size: 5965 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2588 928 Size: 971 689 4305 Fragments arranged by size: 4305 971 689 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3215 3249 4156 4190 1903 Size: 34 1278 34 907 34 3678 Fragments arranged by size: 3678 1278 907 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 1548 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3008 3027 3047 Size: 107 122 71 631 605 1336 19 20 Cuts at: 3047 3176 4576 136 Size: 129 1400 1525 Fragments arranged by size: 1525 1400 1336 631 605 129 122 107 71 20 19 BglII A'GATC_T Cuts at: 5960 5960 Size: 5965 BlpI GC'TnA_GC Cuts at: 2496 3382 2496 Size: 886 5079 Bme1580I G_kGCm'C Cuts at: 1790 2231 2509 2993 3043 4026 5182 1790 Size: 441 278 484 50 983 1156 2573 Fragments arranged by size: 2573 1156 983 484 441 278 50 BmgBI CAC'GTC Cuts at: 1318 2265 1318 Size: 947 5018 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3124 3696 4654 Size: 416 53 83 399 24 1707 572 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1707 958 572 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 4625 1239 Size: 1031 2355 2579 Fragments arranged by size: 2579 2355 1031 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4090 4955 5253 4090 Size: 865 298 4802 Fragments arranged by size: 4802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 5959 5959 Size: 5965 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2189 2521 2774 2880 Size: 53 83 186 936 656 332 253 106 Cuts at: 2880 3179 3772 4154 4789 275 Size: 299 593 382 635 1451 Fragments arranged by size: 1451 936 656 635 593 382 332 299 253 186 106 83 53 BsaWI w'CCGG_w Cuts at: 4398 4796 5070 5217 5628 4398 Size: 398 274 147 411 4735 Fragments arranged by size: 4735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2870 2900 1178 Size: 30 165 30 1467 30 4243 Fragments arranged by size: 4243 1467 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2939 1186 Size: 1753 4212 BseYI C'CCAG_C Cuts at: 2224 2349 2815 2824 2859 3067 5168 5664 2224 Size: 125 466 9 35 208 2101 496 2525 Fragments arranged by size: 2525 2101 496 466 208 125 35 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3202 1163 Size: 573 1466 3926 Fragments arranged by size: 3926 1466 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2370 2743 2851 2952 3031 3148 Size: 275 186 369 373 108 101 79 117 Cuts at: 3148 3415 4176 5204 1540 Size: 267 761 1028 2301 Fragments arranged by size: 2301 1028 761 373 369 275 267 186 117 108 101 79 BsiHKAI G_wGCw'C Cuts at: 2319 3238 4026 4111 5182 2319 Size: 919 788 85 1071 3102 Fragments arranged by size: 3102 1071 919 788 85 BsmI GAATG_Cn' Cuts at: 3096 3476 3569 3096 Size: 380 93 5492 Fragments arranged by size: 5492 380 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2512 2584 2512 Size: 72 5893 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2231 2285 2319 2509 2993 3043 3084 Size: 399 441 54 34 190 484 50 41 Cuts at: 3084 3238 3284 4026 4111 5182 1391 Size: 154 46 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 441 399 190 154 85 54 50 46 41 34 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 3854 5584 3854 Size: 1730 4235 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2568 3168 3230 3320 844 Size: 1724 600 62 90 3489 Fragments arranged by size: 3489 1724 600 90 62 BsrBI CCG'CTC Cuts at: 1394 1823 3121 3150 3241 3852 1394 Size: 429 1298 29 91 611 3507 Fragments arranged by size: 3507 1298 611 429 91 29 BsrDI GCAATG_nn' Cuts at: 65 1248 4460 4634 65 Size: 1183 3212 174 1396 Fragments arranged by size: 3212 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2362 2369 2846 4609 4796 1700 Size: 110 552 7 477 1763 187 2869 Fragments arranged by size: 2869 1763 552 477 187 110 7 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 2150 1925 Size: 225 5740 BssSI C'ACGA_G Cuts at: 3231 4025 5037 3231 Size: 794 1012 4159 Fragments arranged by size: 4159 1012 794 BstAPI GCAn_nnn'nTGC Cuts at: 794 2976 794 Size: 2182 3783 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstXI CCAn_nnnn'nTGG Cuts at: 3067 3182 3067 Size: 115 5850 BstYI r'GATC_y Cuts at: 1972 2195 3295 3662 4047 4064 5516 5602 5614 Size: 223 1100 367 385 17 1452 86 12 Cuts at: 5614 5960 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1100 385 367 346 223 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2432 2432 Size: 5965 BtgI C'CryG_G Cuts at: 513 1065 2208 2544 3012 3022 3089 513 Size: 552 1143 336 468 10 67 3389 Fragments arranged by size: 3389 1143 552 468 336 67 10 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 2854 2946 3481 4275 4295 2854 Size: 92 535 794 20 4524 Fragments arranged by size: 4524 794 535 92 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2848 2949 3028 Size: 54 1425 50 275 18 1018 101 79 Cuts at: 3028 3145 3412 4301 5930 8 Size: 117 267 889 1629 43 Fragments arranged by size: 1629 1425 1018 889 275 267 117 101 79 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2848 2949 3028 3145 3412 1537 Size: 275 1036 101 79 117 267 4090 Fragments arranged by size: 4090 1036 275 267 117 101 79 EarI CTCTTCn'nnn_ Cuts at: 1353 2039 2905 3895 1353 Size: 686 866 990 3423 Fragments arranged by size: 3423 990 866 686 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2086 2349 2725 4552 4926 5072 Size: 1064 138 497 263 376 1827 374 146 Cuts at: 5072 387 Size: 1280 Fragments arranged by size: 1827 1280 1064 497 376 374 263 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2664 4028 4625 5412 5680 1239 Size: 393 638 394 1364 597 787 268 1524 Fragments arranged by size: 1524 1364 787 638 597 394 393 268 EcoO109I rG'GnC_Cy Cuts at: 2293 2505 3040 2293 Size: 212 535 5218 Fragments arranged by size: 5218 535 212 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FseI GG_CCGG'CC Cuts at: 2850 2850 Size: 5965 FspI TGC'GCA Cuts at: 2832 4471 2832 Size: 1639 4326 HaeII r_GCGC'y Cuts at: 1678 2803 3182 5112 1678 Size: 1125 379 1930 2531 Fragments arranged by size: 2531 1930 1125 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2019 2040 2051 2072 2254 Size: 32 135 32 447 21 11 21 182 Cuts at: 2254 2286 2586 2618 2870 2902 3134 3166 4606 Size: 32 300 32 252 32 232 32 1440 Cuts at: 4606 4638 4680 4712 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1440 447 300 252 232 182 135 42 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 3309 3555 669 Size: 988 281 1371 246 3079 Fragments arranged by size: 3079 1371 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5965 HpaI GTT'AAC Cuts at: 3555 3555 Size: 5965 KasI G'GCGC_C Cuts at: 3178 3178 Size: 5965 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2080 5079 5263 1305 Size: 775 2999 184 2007 Fragments arranged by size: 2999 2007 775 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4143 Fragments arranged by size: 4143 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3101 3923 4282 4441 518 Size: 648 1935 822 359 159 2042 Fragments arranged by size: 2042 1935 822 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2349 2819 3024 3091 3211 3257 3381 Size: 651 92 470 205 67 120 46 124 Cuts at: 3381 4058 5206 5451 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 470 245 205 124 120 92 67 46 NaeI GCC'GGC Cuts at: 2364 2848 2364 Size: 484 5481 NarI GG'CG_CC Cuts at: 3179 3179 Size: 5965 NcoI C'CATG_G Cuts at: 513 1065 2208 2544 513 Size: 552 1143 336 3934 Fragments arranged by size: 3934 1143 552 336 NdeI CA'TA_TG Cuts at: 387 387 Size: 5965 NgoMIV G'CCGG_C Cuts at: 2362 2846 2362 Size: 484 5481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3028 3412 3028 Size: 384 5581 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 2705 3190 1348 Size: 1357 485 4123 Fragments arranged by size: 4123 1357 485 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2199 1108 Size: 902 189 4874 Fragments arranged by size: 4874 902 189 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2293 Size: 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PspOMI G'GGCC_C Cuts at: 2227 2505 3039 2227 Size: 278 534 5153 Fragments arranged by size: 5153 534 278 PstI C_TGCA'G Cuts at: 830 2894 3317 830 Size: 2064 423 3478 Fragments arranged by size: 3478 2064 423 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 PvuII CAG'CTG Cuts at: 2257 2819 2257 Size: 562 5403 SacII CC_GC'GG Cuts at: 3025 3092 3025 Size: 67 5898 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 4213 5925 4213 Size: 1712 4253 SfcI C'TryA_G Cuts at: 826 1045 2890 3313 4448 5129 5320 826 Size: 219 1845 423 1135 681 191 1471 Fragments arranged by size: 1845 1471 1135 681 423 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3008 3027 3008 Size: 19 5946 SfoI GGC'GCC Cuts at: 3180 3180 Size: 5965 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 2362 1700 Size: 662 5303 SmaI CCC'GGG Cuts at: 1872 2232 3292 1872 Size: 360 1060 4545 Fragments arranged by size: 4545 1060 360 SmlI C'TyrA_G Cuts at: 820 1017 1943 4069 4970 5232 820 Size: 197 926 2126 901 262 1553 Fragments arranged by size: 2126 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2208 2544 3404 513 Size: 552 1143 336 860 3074 Fragments arranged by size: 3074 1143 860 552 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2816 3992 4009 4162 4347 4792 325 Size: 1255 1236 1176 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1236 1176 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4211 5923 96 Size: 274 80 33 51 1443 2234 1712 138 Fragments arranged by size: 2234 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2380 2412 5925 5957 2380 Size: 32 3513 32 2388 Fragments arranged by size: 3513 2388 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2551 2551 Size: 5965 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 2230 3290 1870 Size: 360 1060 4545 Fragments arranged by size: 4545 1060 360 XmnI GAAnn'nnTTC Cuts at: 4094 4094 Size: 5965 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SrfI SwaI