(Circular) (Six-base) MAPSORT of: pFN21AB7178.seq Check: 422 from: 1 to: 5983 pFN21AB7178 5983 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2314 3326 1937 Size: 377 1012 4594 Fragments arranged by size: 4594 1012 377 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2903 4046 5430 5698 1632 Size: 1271 1143 1384 268 1917 Fragments arranged by size: 1917 1384 1271 1143 268 AflII C'TTAA_G Cuts at: 820 1017 2416 820 Size: 197 1399 4387 Fragments arranged by size: 4387 1399 197 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 4814 4814 Size: 5983 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2894 1166 Size: 1728 4255 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2565 1184 Size: 1381 4602 AlwNI CAG_nnn'CTG Cuts at: 2935 3726 5298 2935 Size: 791 1572 3620 Fragments arranged by size: 3620 1572 791 ApaI G_GGCC'C Cuts at: 2662 2662 Size: 5983 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 2062 3292 3518 3670 5779 1493 Size: 569 1230 226 152 2109 1697 Fragments arranged by size: 2109 1697 1230 569 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2745 3308 1870 Size: 73 802 563 4545 Fragments arranged by size: 4545 802 563 73 BamHI G'GATC_C Cuts at: 3313 3680 3313 Size: 367 5616 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2828 3304 4759 618 Size: 325 217 627 225 816 476 1455 1842 Fragments arranged by size: 1842 1455 816 627 476 325 225 217 BanII G_rGCy'C Cuts at: 1391 2662 3302 1391 Size: 1271 640 4072 Fragments arranged by size: 4072 1271 640 BbeI G_GCGC'C Cuts at: 2832 2832 Size: 5983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 2267 2516 928 Size: 971 315 53 249 4395 Fragments arranged by size: 4395 971 315 249 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2723 2757 4174 4208 1903 Size: 34 786 34 1417 34 3678 Fragments arranged by size: 3678 1417 786 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3875 5085 3875 Size: 1210 4773 BclI T'GATC_A Cuts at: 1548 1548 Size: 5983 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 4594 136 Size: 107 122 71 631 605 985 1937 1525 Fragments arranged by size: 1937 1525 985 631 605 122 107 71 BglII A'GATC_T Cuts at: 2919 5978 2919 Size: 3059 2924 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2662 4044 5200 1790 Size: 872 1382 1156 2573 Fragments arranged by size: 2573 1382 1156 872 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2665 3112 3714 Size: 416 53 83 399 24 1248 447 602 Cuts at: 3714 4672 442 Size: 958 1753 Fragments arranged by size: 1753 1248 958 602 447 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2058 4643 1239 Size: 819 2585 2579 Fragments arranged by size: 2585 2579 819 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 4108 4973 5271 2090 Size: 2018 865 298 2802 Fragments arranged by size: 2802 2018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2624 4646 882 Size: 617 1125 2022 2219 Fragments arranged by size: 2219 2022 1125 617 BsaAI yAC'GTr Cuts at: 493 1204 2341 2587 2800 2878 3774 493 Size: 711 1137 246 213 78 896 2702 Fragments arranged by size: 2702 1137 896 711 246 213 78 BsaBI GATnn'nnATC Cuts at: 5977 5977 Size: 5983 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2534 2829 3790 4172 Size: 53 83 186 936 1001 295 961 382 Cuts at: 4172 4807 275 Size: 635 1451 Fragments arranged by size: 1451 1001 961 936 635 382 295 186 83 53 BsaWI w'CCGG_w Cuts at: 4416 4814 5088 5235 5646 4416 Size: 398 274 147 411 4753 Fragments arranged by size: 4753 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2666 2696 1178 Size: 30 165 30 1263 30 4465 Fragments arranged by size: 4465 1263 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2543 1186 Size: 1357 4626 BseYI C'CCAG_C Cuts at: 2910 5186 5682 2910 Size: 2276 496 3211 Fragments arranged by size: 3211 2276 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5410 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3433 4194 5222 1540 Size: 275 186 1432 761 1028 2301 Fragments arranged by size: 2301 1432 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2697 2805 4044 4129 5200 2697 Size: 108 1239 85 1071 3480 Fragments arranged by size: 3480 1239 1071 108 85 BsmI GAATG_Cn' Cuts at: 2623 3494 3587 2623 Size: 871 93 5019 Fragments arranged by size: 5019 871 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2525 2525 Size: 5983 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2662 2697 2805 3302 4044 4129 5200 Size: 399 872 35 108 497 742 85 1071 Cuts at: 5200 1391 Size: 2174 Fragments arranged by size: 2174 1071 872 742 497 399 108 85 35 BspEI T'CCGG_A Cuts at: 5646 5646 Size: 5983 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3338 844 Size: 2494 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4627 4814 1700 Size: 110 2817 187 2869 Fragments arranged by size: 2869 2817 187 110 BsrGI T'GTAC_A Cuts at: 96 2444 96 Size: 2348 3635 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 4043 5055 4043 Size: 1012 4971 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 794 Size: 2021 3962 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 2887 1184 Size: 1703 4280 BstXI CCAn_nnnn'nTGG Cuts at: 2479 2845 2479 Size: 366 5617 BstYI r'GATC_y Cuts at: 1972 2376 2919 3313 3680 4065 4082 5534 5620 Size: 404 543 394 367 385 17 1452 86 Cuts at: 5620 5632 5978 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 543 404 394 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2944 513 Size: 552 939 940 3552 Fragments arranged by size: 3552 940 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2629 487 Size: 683 56 1403 3841 Fragments arranged by size: 3841 1403 683 56 BtsI GCAGTG_nn' Cuts at: 2016 3499 4293 4313 2016 Size: 1483 794 20 3686 Fragments arranged by size: 3686 1483 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 2761 3287 2761 Size: 526 5457 DraIII CAC_nnn'GTG Cuts at: 3063 3063 Size: 5983 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4990 809 Size: 507 3674 1802 Fragments arranged by size: 3674 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2260 2355 2640 Size: 54 1425 50 275 18 430 95 285 Cuts at: 2640 3430 4319 5948 8 Size: 790 889 1629 43 Fragments arranged by size: 1629 1425 889 790 430 285 275 95 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3430 1537 Size: 275 1618 4090 Fragments arranged by size: 4090 1618 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 3064 3913 1353 Size: 987 724 849 3423 Fragments arranged by size: 3423 987 849 724 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2058 2903 4046 4643 5430 5698 1239 Size: 393 426 845 1143 597 787 268 1524 Fragments arranged by size: 1524 1143 845 787 597 426 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2774 2774 Size: 5983 EcoO109I rG'GnC_Cy Cuts at: 2659 2659 Size: 5983 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 EcoRV GAT'ATC Cuts at: 2851 2851 Size: 5983 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 2078 2832 5130 1678 Size: 400 754 2298 2531 Fragments arranged by size: 2531 2298 754 400 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2747 2779 4624 4656 4698 Size: 32 135 32 1175 32 1845 32 42 Cuts at: 4698 4730 1373 Size: 32 2626 Fragments arranged by size: 2626 1845 1175 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KasI G'GCGC_C Cuts at: 2828 2828 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 2404 2477 3015 3582 2404 Size: 73 538 567 4805 Fragments arranged by size: 4805 567 538 73 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5097 5281 1305 Size: 3792 184 2007 Fragments arranged by size: 3792 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2262 2357 2642 10 Size: 54 1425 343 430 95 285 3351 Fragments arranged by size: 3351 1425 430 343 285 95 54 MslI CAynn'nnrTG Cuts at: 518 1166 2810 2894 3941 4300 4459 518 Size: 648 1644 84 1047 359 159 2042 Fragments arranged by size: 2042 1644 1047 648 359 159 84 MspA1I CmG'CkG Cuts at: 1606 3399 4076 5224 5469 1606 Size: 1793 677 1148 245 2120 Fragments arranged by size: 2120 1793 1148 677 245 NarI GG'CG_CC Cuts at: 2829 2829 Size: 5983 NcoI C'CATG_G Cuts at: 513 1065 2004 2944 513 Size: 552 939 940 3552 Fragments arranged by size: 3552 940 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2716 3341 4886 1132 Size: 97 1487 625 1545 2229 Fragments arranged by size: 2229 1545 1487 625 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PmlI CAC'GTG Cuts at: 2341 2800 2341 Size: 459 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 2184 3553 2184 Size: 1369 4614 PspOMI G'GGCC_C Cuts at: 2658 2658 Size: 5983 PstI C_TGCA'G Cuts at: 830 3335 830 Size: 2505 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 3064 3064 Size: 5983 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 3251 4231 5943 3251 Size: 980 1712 3291 Fragments arranged by size: 3291 1712 980 SexAI A'CCwGG_T Cuts at: 2700 2700 Size: 5983 SfcI C'TryA_G Cuts at: 826 1045 2311 3331 4466 5147 5338 826 Size: 219 1266 1020 1135 681 191 1471 Fragments arranged by size: 1471 1266 1135 1020 681 219 191 SfoI GGC'GCC Cuts at: 2830 2830 Size: 5983 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2069 2416 4087 4988 5250 820 Size: 197 926 126 347 1671 901 262 1553 Fragments arranged by size: 1671 1553 926 901 347 262 197 126 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 2716 3341 1132 Size: 1584 625 3774 Fragments arranged by size: 3774 1584 625 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2238 2993 1517 Size: 721 755 4507 Fragments arranged by size: 4507 755 721 StyI C'CwwG_G Cuts at: 513 1065 2004 2944 3146 3422 513 Size: 552 939 940 202 276 3074 Fragments arranged by size: 3074 940 939 552 276 202 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2444 2682 3249 Size: 274 80 33 51 1443 467 238 567 Cuts at: 3249 4229 5941 96 Size: 980 1712 138 Fragments arranged by size: 1712 1443 980 567 467 274 238 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5943 5975 5943 Size: 32 5951 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5983 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2274 4112 2274 Size: 1838 4145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SrfI SwaI XcmI