Restriction map for
pFN21AB7176
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII BamHI BbeI BbrPI BcgI
BciVI BclI BfrI BglII BlpI
BpmI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DsaI Eam1105I Eco47III Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FspI
HindIII HpaI KasI KspI MaeIII
MamI MfeI MflI MluI MroI
MseI MunI NarI NcoI NdeI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI ScaI SexAI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 80 GGTACC G^GTACC
AccI 328 GTCGAC GT^[AC][GT]AC
AccII 407 CGCG CG^CG
AcyI 982 GACGCC G[GA]^CG[CT]C
AfaI 81 GTAC GT^AC
AlwI 342 GATCC GGATC(4/5)
Asp718I 80 GGTACC G^GTACC
AvrII 739 CCTAGG C^CTAGG
BalI 116 TGGCCA TGG^CCA
BbsI 214 GAAGAC GAAGAC(2/6)
BbvCI 349 GCTGAGG CCTCAGC(-5/-2)
BlnI 739 CCTAGG C^CTAGG
BpuAI 214 GAAGAC GAAGAC(2/6)
BsaAI 439 TACGTG [CT]AC^GT[GA]
BsaHI 982 GACGCC G[GA]^CG[CT]C
BsmAI 691 GAGAC GTCTC(1/5)
BspMI 83 ACCTGC ACCTGC(4/8)
BsrBI 143 CCGCTC CCGCTC(-3/-3)
BssSI 675 CACGAG CACGAG(-5/-1)
BstF5I 177 CATCC GGATG(2/0)
BstUI 407 CGCG CG^CG
DraIII 485 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1024 GACGTTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FokI 177 CATCC GGATG(9/13)
FseI 451 GGCCGGCC GGCCGG^CC
HgaI 982 GACGC GACGC(5/10)
Hin1I 982 GACGCC G[GA]^CG[CT]C
HphI 132 GGTGA GGTGA(8/7)
KpnI 80 GGTACC GGTAC^C
MluNI 116 TGGCCA TGG^CCA
MscI 116 TGGCCA TGG^CCA
MslI 675 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 407 CGCG CG^CG
NheI 552 GCTAGC G^CTAGC
RsaI 81 GTAC GT^AC
SalI 328 GTCGAC G^TCGAC
SbfI 577 CCTGCAGG CCTGCA^GG
Sse8387I 577 CCTGCAGG CCTGCA^GG
StuI 640 AGGCCT AGG^CCT
TaqI 329 TCGA T^CGA
TfiI 135 GATTC G^A[AT]TC
TthHB8I 329 TCGA T^CGA
XbaI 978 TCTAGA T^CTAGA
XcmI 119 CCATGATCGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwNI 572 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
776 CAGTTCCTG
AvaI 950 CCCGAG C^[CT]CG[GA]G
1043 CTCGGG
BcnI 527 CCGGG CC^[GC]GG
547 CCGGG
BseRI 66 GAGGAG GAGGAG(10/8)
601 GAGGAG
BsoBI 950 CCCGAG C^[CT]CG[GA]G
1043 CTCGGG
BstXI 422 CCAAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
674 CCACGAGAGTGG
EagI 450 CGGCCG C^GGCCG
463 CGGCCG
EclXI 450 CGGCCG C^GGCCG
463 CGGCCG
Eco52I 450 CGGCCG C^GGCCG
463 CGGCCG
EcoNI 203 CCTTCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1060 CCTGGCCCAGG
HaeII 354 GGCGCT [GA]GCGC^[CT]
386 GGCGCT
HincII 328 GTCGAC GT[CT]^[GA]AC
912 GTCAAC
HindII 328 GTCGAC GT[CT]^[GA]AC
912 GTCAAC
NaeI 383 GCCGGC GCC^GGC
452 GCCGGC
NciI 527 CCGGG CC^[GC]GG
547 CCGGG
NgoMIV 383 GCCGGC G^CCGGC
452 GCCGGC
NlaIII 91 CATG CATG^
120 CATG
PleI 235 GACTC GAGTC(4/5)
43 GAGTC
PvuII 347 CAGCTG CAG^CTG
826 CAGCTG
SfiI 455 GGCCATACCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
641 GGCCTCTGTGGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AluI ApaI AvaII BanI
BanII BbvI BfaI BglI BmyI
BsaJI BsgI BsiEI BsiYI BslI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
HaeIII HapII HhaI HinP1I HinfI
HpaII ItaI Ksp632I MaeI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIV PpuMI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TseI TspRI