(Circular) (Six-base) MAPSORT of: pFN21AB7176.seq Check: 6467 from: 1 to: 5800 pFN21AB7176 5800 bp With 182 enzymes: SgfI * February 27, 2009 14:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3610 278 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2334 3143 1937 Size: 397 809 4594 Fragments arranged by size: 4594 809 397 Acc65I G'GTAC_C Cuts at: 2085 3121 2085 Size: 1036 4764 AclI AA'CG_TT Cuts at: 1563 3927 4300 1563 Size: 2364 373 3063 Fragments arranged by size: 3063 2364 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2186 2197 2488 3863 5247 5515 1632 Size: 554 11 291 1375 1384 268 1917 Fragments arranged by size: 1917 1384 1375 554 291 268 11 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5603 AflIII A'CryG_T Cuts at: 1203 4699 5676 1203 Size: 3496 977 1327 Fragments arranged by size: 3496 1327 977 AgeI A'CCGG_T Cuts at: 4631 4631 Size: 5800 AhdI GACnn_n'nnGTC Cuts at: 4529 4529 Size: 5800 AleI CACnn'nnGTG Cuts at: 1166 2684 1166 Size: 1518 4282 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5800 AlwNI CAG_nnn'CTG Cuts at: 2582 2786 3543 5115 2582 Size: 204 757 1572 3267 Fragments arranged by size: 3267 1572 757 204 ApaI G_GGCC'C Cuts at: 2013 2037 2441 2724 2964 2013 Size: 24 404 283 240 4849 Fragments arranged by size: 4849 404 283 240 24 ApaLI G'TGCA_C Cuts at: 3857 5013 3857 Size: 1156 4644 ApoI r'AATT_y Cuts at: 1493 3109 3335 3487 5596 1493 Size: 1616 226 152 2109 1697 Fragments arranged by size: 2109 1697 1616 226 152 AseI AT'TA_AT Cuts at: 160 1032 4354 160 Size: 872 3322 1606 Fragments arranged by size: 3322 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 AvaI C'yCGr_G Cuts at: 1870 1943 2955 3048 3125 1870 Size: 73 1012 93 77 4545 Fragments arranged by size: 4545 1012 93 77 73 AvrII C'CTAG_G Cuts at: 2744 2744 Size: 5800 BamHI G'GATC_C Cuts at: 3130 3497 3130 Size: 367 5433 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2085 2284 2542 2614 2774 Size: 325 217 627 298 199 258 72 160 Cuts at: 2774 2884 3004 3121 4576 618 Size: 110 120 117 1455 1842 Fragments arranged by size: 1842 1455 627 325 298 258 217 199 160 120 117 110 72 BanII G_rGCy'C Cuts at: 1391 2013 2037 2441 2724 2964 3119 1391 Size: 622 24 404 283 240 155 4072 Fragments arranged by size: 4072 622 404 283 240 155 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 928 Size: 971 327 4502 Fragments arranged by size: 4502 971 327 BbvCI CC'TCA_GC Cuts at: 2355 2355 Size: 5800 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3991 4025 1903 Size: 34 2054 34 3678 Fragments arranged by size: 3678 2054 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3692 4902 3692 Size: 1210 4590 BclI T'GATC_A Cuts at: 1548 1548 Size: 5800 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2467 2653 2968 Size: 107 122 71 631 605 795 186 315 Cuts at: 2968 4411 136 Size: 1443 1525 Fragments arranged by size: 1525 1443 795 631 605 315 186 122 107 71 BglII A'GATC_T Cuts at: 5795 5795 Size: 5800 BlpI GC'TnA_GC Cuts at: 3217 3217 Size: 5800 Bme1580I G_kGCm'C Cuts at: 1790 2013 2037 2289 2441 2545 2619 2724 2777 Size: 223 24 252 152 104 74 105 53 Cuts at: 2777 2964 3861 5017 1790 Size: 187 897 1156 2573 Fragments arranged by size: 2573 1156 897 252 223 187 152 105 104 74 53 24 BmgBI CAC'GTC Cuts at: 1318 2338 1318 Size: 1020 4780 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2469 2487 2570 Size: 416 53 83 399 24 1052 18 83 Cuts at: 2570 2902 2944 3531 4489 442 Size: 332 42 587 958 1753 Fragments arranged by size: 1753 1052 958 587 416 399 332 83 83 53 42 24 18 BmtI G_CTAG'C Cuts at: 1056 2561 1056 Size: 1505 4295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2071 2071 Size: 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4460 1239 Size: 3221 2579 Bpu10I CC'TnA_GC Cuts at: 2355 2355 Size: 5800 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3925 4790 5088 3925 Size: 865 298 4637 Fragments arranged by size: 4637 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4463 882 Size: 617 2964 2219 Fragments arranged by size: 2964 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2446 3591 493 Size: 711 1242 1145 2702 Fragments arranged by size: 2702 1242 1145 711 BsaBI GATnn'nnATC Cuts at: 5794 5794 Size: 5800 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2988 3607 3989 4624 Size: 53 83 186 936 1455 619 382 635 Cuts at: 4624 275 Size: 1451 Fragments arranged by size: 1455 1451 936 635 619 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4233 4631 4905 5052 5463 4233 Size: 398 274 147 411 4570 Fragments arranged by size: 4570 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2246 2276 2801 2831 1178 Size: 30 165 30 843 30 525 30 4147 Fragments arranged by size: 4147 843 525 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2086 2621 1186 Size: 900 535 4365 Fragments arranged by size: 4365 900 535 BseYI C'CCAG_C Cuts at: 2058 2350 5003 5499 2058 Size: 292 2653 496 2359 Fragments arranged by size: 2653 2359 496 292 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 2723 3112 1163 Size: 573 699 288 389 3851 Fragments arranged by size: 3851 699 573 389 288 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2334 2458 2471 3250 4011 5039 Size: 275 186 333 124 13 779 761 1028 Cuts at: 5039 1540 Size: 2301 Fragments arranged by size: 2301 1028 779 761 333 275 186 124 13 BsiHKAI G_wGCw'C Cuts at: 3861 3946 5017 3861 Size: 85 1071 4644 Fragments arranged by size: 4644 1071 85 BsmI GAATG_Cn' Cuts at: 3311 3404 3311 Size: 93 5707 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2037 2289 2441 2545 2619 2724 Size: 399 223 24 252 152 104 74 105 Cuts at: 2724 2777 2964 3119 3861 3946 5017 1391 Size: 53 187 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 252 223 187 155 152 105 104 85 74 53 24 BspEI T'CCGG_A Cuts at: 5463 5463 Size: 5800 BspHI T'CATG_A Cuts at: 3689 5419 3689 Size: 1730 4070 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2097 3155 844 Size: 1253 1058 3489 Fragments arranged by size: 3489 1253 1058 BsrBI CCG'CTC Cuts at: 1394 1823 2150 3687 1394 Size: 429 327 1537 3507 Fragments arranged by size: 3507 1537 429 327 BsrDI GCAATG_nn' Cuts at: 65 1248 4295 4469 65 Size: 1183 3047 174 1396 Fragments arranged by size: 3047 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2388 2457 2466 4444 4631 1700 Size: 110 578 69 9 1978 187 2869 Fragments arranged by size: 2869 1978 578 187 110 69 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5800 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5800 BssSI C'ACGA_G Cuts at: 2680 3860 4872 2680 Size: 1180 1012 3608 Fragments arranged by size: 3608 1180 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5800 BstBI TT'CG_AA Cuts at: 4604 4604 Size: 5800 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5800 BstXI CCAn_nnnn'nTGG Cuts at: 2434 2686 2434 Size: 252 5548 BstYI r'GATC_y Cuts at: 1972 3130 3497 3882 3899 5351 5437 5449 5795 Size: 1158 367 385 17 1452 86 12 346 Cuts at: 5795 1972 Size: 1977 Fragments arranged by size: 1977 1452 1158 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5061 Fragments arranged by size: 5061 683 56 BtsI GCAGTG_nn' Cuts at: 3316 4110 4130 3316 Size: 794 20 4986 Fragments arranged by size: 4986 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5800 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5765 DraI TTT'AAA Cuts at: 3104 3104 Size: 5800 DraIII CAC_nnn'GTG Cuts at: 2495 2495 Size: 5800 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3035 4807 809 Size: 507 1719 1772 1802 Fragments arranged by size: 1802 1772 1719 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2455 2459 Size: 54 1425 50 275 18 291 334 4 Cuts at: 2459 2468 2549 3247 4136 5765 8 Size: 9 81 698 889 1629 43 Fragments arranged by size: 1629 1425 889 698 334 291 275 81 54 50 43 18 9 4 EagI C'GGCC_G Cuts at: 1537 1812 2455 2468 3247 1537 Size: 275 643 13 779 4090 Fragments arranged by size: 4090 779 643 275 13 EarI CTCTTCn'nnn_ Cuts at: 1353 2075 2641 2999 3730 1353 Size: 722 566 358 731 3423 Fragments arranged by size: 3423 731 722 566 358 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4387 4761 4907 387 Size: 1064 138 2798 374 146 1280 Fragments arranged by size: 2798 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 2197 2488 3863 4460 5247 5515 Size: 393 554 11 291 1375 597 787 268 Cuts at: 5515 1239 Size: 1524 Fragments arranged by size: 1524 1375 787 597 554 393 291 268 11 EcoNI CCTnn'n_nnAGG Cuts at: 2212 3069 2212 Size: 857 4943 EcoO109I rG'GnC_Cy Cuts at: 2009 2010 2033 2034 2293 2431 2438 2721 2748 Size: 1 23 1 259 138 7 283 27 Cuts at: 2748 2814 2960 2961 3052 2009 Size: 66 146 1 91 4757 Fragments arranged by size: 4757 283 259 146 138 91 66 27 23 7 1 1 1 EcoRI G'AATT_C Cuts at: 3109 3487 3109 Size: 378 5422 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2338 2338 Size: 5800 FseI GG_CCGG'CC Cuts at: 2461 2461 Size: 5800 FspI TGC'GCA Cuts at: 4306 4306 Size: 5800 HaeII r_GCGC'y Cuts at: 1678 2363 2395 4947 1678 Size: 685 32 2552 2531 Fragments arranged by size: 2552 2531 685 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2033 2065 2170 2202 4441 Size: 32 135 32 461 32 105 32 2239 Cuts at: 4441 4473 4515 4547 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2239 461 135 105 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 2919 3144 3390 669 Size: 988 281 397 584 225 246 3079 Fragments arranged by size: 3079 988 584 397 281 246 225 HindIII A'AGCT_T Cuts at: 748 748 Size: 5800 HpaI GTT'AAC Cuts at: 3390 3390 Size: 5800 KpnI G_GTAC'C Cuts at: 2089 3125 2089 Size: 1036 4764 MfeI C'AATT_G Cuts at: 3399 3399 Size: 5800 MluI A'CGCG_T Cuts at: 5676 5676 Size: 5800 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2289 4914 5098 1305 Size: 984 2625 184 2007 Fragments arranged by size: 2625 2007 984 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 10 Size: 54 1425 343 291 3687 Fragments arranged by size: 3687 1425 343 291 54 MslI CAynn'nnrTG Cuts at: 518 1166 2684 3758 4117 4276 518 Size: 648 1518 1074 359 159 2042 Fragments arranged by size: 2042 1518 1074 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 2354 2802 2833 3216 3893 5041 5286 Size: 413 335 448 31 383 677 1148 245 Cuts at: 5286 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 448 413 383 335 245 31 NaeI GCC'GGC Cuts at: 2390 2459 2390 Size: 69 5731 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4309 Fragments arranged by size: 4309 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5800 NgoMIV G'CCGG_C Cuts at: 2388 2457 2388 Size: 69 5731 NheI G'CTAG_C Cuts at: 1052 2557 1052 Size: 1505 4295 NotI GC'GGCC_GC Cuts at: 3247 3247 Size: 5800 NspI r_CATG'y Cuts at: 1132 1229 3158 4703 1132 Size: 97 1929 1545 2229 Fragments arranged by size: 2229 1929 1545 97 PasI CC'CwG_GG Cuts at: 1348 2966 1348 Size: 1618 4182 PciI A'CATG_T Cuts at: 4699 4699 Size: 5800 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5800 PmeI GTTT'AAAC Cuts at: 3104 3104 Size: 5800 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3869 3901 5382 5414 3869 Size: 32 1481 32 4255 Fragments arranged by size: 4255 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2431 3052 2293 Size: 138 621 5041 Fragments arranged by size: 5041 621 138 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5800 PsiI TTA'TAA Cuts at: 3370 3370 Size: 5800 PspOMI G'GGCC_C Cuts at: 2009 2033 2437 2720 2960 2009 Size: 24 404 283 240 4849 Fragments arranged by size: 4849 404 283 240 24 PstI C_TGCA'G Cuts at: 830 2018 2373 2587 2772 3152 830 Size: 1188 355 214 185 380 3478 Fragments arranged by size: 3478 1188 380 355 214 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5800 PvuII CAG'CTG Cuts at: 2354 2833 2354 Size: 479 5321 SalI G'TCGA_C Cuts at: 1936 2333 3142 1936 Size: 397 809 4594 Fragments arranged by size: 4594 809 397 SbfI CC_TGCA'GG Cuts at: 2587 3152 2587 Size: 565 5235 ScaI AGT'ACT Cuts at: 4048 5760 4048 Size: 1712 4088 SfcI C'TryA_G Cuts at: 826 1045 2014 2369 2583 2768 3148 4283 4964 Size: 219 969 355 214 185 380 1135 681 Cuts at: 4964 5155 826 Size: 191 1471 Fragments arranged by size: 1471 1135 969 681 380 355 219 214 191 185 SfiI GGCCn_nnn'nGGCC Cuts at: 2467 2653 2467 Size: 186 5614 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5800 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5800 SmaI CCC'GGG Cuts at: 1872 3127 1872 Size: 1255 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3904 4805 5067 820 Size: 197 926 1961 901 262 1553 Fragments arranged by size: 1961 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3591 493 Size: 3098 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5800 SphI G_CATG'C Cuts at: 1132 3158 1132 Size: 2026 3774 SspI AAT'ATT Cuts at: 5 52 3724 5 Size: 47 3672 2081 Fragments arranged by size: 3672 2081 47 StuI AGG'CCT Cuts at: 1517 2647 1517 Size: 1130 4670 StyI C'CwwG_G Cuts at: 513 1065 2004 2133 2289 2313 2427 2744 2880 Size: 552 939 129 156 24 114 317 136 Cuts at: 2880 2998 3239 513 Size: 118 241 3074 Fragments arranged by size: 3074 939 552 317 241 156 136 129 118 114 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2506 2908 3025 3827 3844 3997 4182 Size: 1255 926 402 117 802 17 153 185 Cuts at: 4182 4627 325 Size: 445 1498 Fragments arranged by size: 1498 1255 926 802 445 402 185 153 117 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4046 5758 96 Size: 274 80 33 51 1443 2069 1712 138 Fragments arranged by size: 2069 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2243 2275 2767 2799 2801 2833 2887 2919 5760 Size: 32 492 32 2 32 54 32 2841 Cuts at: 5760 5792 2243 Size: 32 2251 Fragments arranged by size: 2841 2251 492 54 32 32 32 32 32 2 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5800 XbaI T'CTAG_A Cuts at: 2983 3136 2983 Size: 153 5647 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2131 2131 Size: 5800 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5800 XmaI C'CCGG_G Cuts at: 1870 3125 1870 Size: 1255 4545 XmnI GAAnn'nnTTC Cuts at: 3929 3929 Size: 5800 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3608 276 Size: 53 83 186 936 2074 2468 Fragments arranged by size: 2468 2074 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI