Restriction map for
pFN21AB7176
(Linear) (Six-base) MAPSORT of: KIBB7176.seq Check: 6822 from: 1 to: 1095
KIBB7176.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AccI GT'mk_AC
Cuts at: 0 329 1095
Size: 329 766
Acc65I G'GTAC_C
Cuts at: 0 80 1095
Size: 80 1015
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 192 483 1095
Size: 181 11 291 612
Fragments arranged by size:
612 291 181 11
AleI CACnn'nnGTG
Cuts at: 0 679 1095
Size: 679 416
AlwNI CAG_nnn'CTG
Cuts at: 0 577 781 1095
Size: 577 204 314
Fragments arranged by size:
577 314 204
ApaI G_GGCC'C
Cuts at: 0 8 32 436 719 959 1095
Size: 8 24 404 283 240 136
Fragments arranged by size:
404 283 240 136 24 8
AvaI C'yCGr_G
Cuts at: 0 950 1043 1095
Size: 950 93 52
Fragments arranged by size:
950 93 52
AvrII C'CTAG_G
Cuts at: 0 739 1095
Size: 739 356
BanI G'GyrC_C
Cuts at: 0 80 279 537 609 769 879 999 1095
Size: 80 199 258 72 160 110 120 96
Fragments arranged by size:
258 199 160 120 110 96 80 72
BanII G_rGCy'C
Cuts at: 0 8 32 436 719 959 1095
Size: 8 24 404 283 240 136
Fragments arranged by size:
404 283 240 136 24 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 1095
Size: 221 874
BbvCI CC'TCA_GC
Cuts at: 0 350 1095
Size: 350 745
BglI GCCn_nnn'nGGC
Cuts at: 0 462 648 963 1095
Size: 462 186 315 132
Fragments arranged by size:
462 315 186 132
Bme1580I G_kGCm'C
Cuts at: 0 8 32 284 436 540 614 719 772
Size: 8 24 252 152 104 74 105 53
Cuts at: 772 959 1095
Size: 187 136
Fragments arranged by size:
252 187 152 136 105 104 74 53
24 8
BmgBI CAC'GTC
Cuts at: 0 333 1095
Size: 333 762
BmrI ACTGGGnnnn_n'
Cuts at: 0 464 482 565 897 939 1095
Size: 464 18 83 332 42 156
Fragments arranged by size:
464 332 156 83 42 18
BmtI G_CTAG'C
Cuts at: 0 556 1095
Size: 556 539
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 66 1095
Size: 66 1029
Bpu10I CC'TnA_GC
Cuts at: 0 350 1095
Size: 350 745
BsaAI yAC'GTr
Cuts at: 0 441 1095
Size: 441 654
BsaHI Gr'CG_yC
Cuts at: 0 983 1095
Size: 983 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 241 271 796 826 1095
Size: 241 30 525 30 269
Fragments arranged by size:
525 269 241 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 81 616 1095
Size: 81 535 479
Fragments arranged by size:
535 479 81
BseYI C'CCAG_C
Cuts at: 0 53 345 1095
Size: 53 292 750
Fragments arranged by size:
750 292 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 718 1095
Size: 430 288 377
Fragments arranged by size:
430 377 288
BsiEI CG_ry'CG
Cuts at: 0 329 453 466 1095
Size: 329 124 13 629
Fragments arranged by size:
629 329 124 13
Bsp1286I G_dGCh'C
Cuts at: 0 8 32 284 436 540 614 719 772
Size: 8 24 252 152 104 74 105 53
Cuts at: 772 959 1095
Size: 187 136
Fragments arranged by size:
252 187 152 136 105 104 74 53
24 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 92 1095
Size: 92 1003
BsrBI CCG'CTC
Cuts at: 0 145 1095
Size: 145 950
BsrFI r'CCGG_y
Cuts at: 0 383 452 461 1095
Size: 383 69 9 634
Fragments arranged by size:
634 383 69 9
BssSI C'ACGA_G
Cuts at: 0 675 1095
Size: 675 420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 429 681 1095
Size: 429 252 414
Fragments arranged by size:
429 414 252
DraIII CAC_nnn'GTG
Cuts at: 0 490 1095
Size: 490 605
DrdI GACnn_nn'nnGTC
Cuts at: 0 1030 1095
Size: 1030 65
EaeI y'GGCC_r
Cuts at: 0 116 450 454 463 544 1095
Size: 116 334 4 9 81 551
Fragments arranged by size:
551 334 116 81 9 4
EagI C'GGCC_G
Cuts at: 0 450 463 1095
Size: 450 13 632
Fragments arranged by size:
632 450 13
EarI CTCTTCn'nnn_
Cuts at: 0 70 636 994 1095
Size: 70 566 358 101
Fragments arranged by size:
566 358 101 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 192 483 1095
Size: 181 11 291 612
Fragments arranged by size:
612 291 181 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 207 1064 1095
Size: 207 857 31
Fragments arranged by size:
857 207 31
EcoO109I rG'GnC_Cy
Cuts at: 0 4 5 28 29 288 426 433 716
Size: 4 1 23 1 259 138 7 283
Cuts at: 716 743 809 955 956 1047 1095
Size: 27 66 146 1 91 48
Fragments arranged by size:
283 259 146 138 91 66 48 27
23 7 4 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 333 1095
Size: 333 762
FseI GG_CCGG'CC
Cuts at: 0 456 1095
Size: 456 639
HaeII r_GCGC'y
Cuts at: 0 358 390 1095
Size: 358 32 705
Fragments arranged by size:
705 358 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 165 197 1095
Size: 28 32 105 32 898
Fragments arranged by size:
898 105 32 32 28
HincII GTy'rAC
Cuts at: 0 330 914 1095
Size: 330 584 181
Fragments arranged by size:
584 330 181
KpnI G_GTAC'C
Cuts at: 0 84 1095
Size: 84 1011
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1095
Size: 284 811
MscI TGG'CCA
Cuts at: 0 118 1095
Size: 118 977
MslI CAynn'nnrTG
Cuts at: 0 679 1095
Size: 679 416
MspA1I CmG'CkG
Cuts at: 0 14 349 797 828 1095
Size: 14 335 448 31 267
Fragments arranged by size:
448 335 267 31 14
NaeI GCC'GGC
Cuts at: 0 385 454 1095
Size: 385 69 641
Fragments arranged by size:
641 385 69
NgoMIV G'CCGG_C
Cuts at: 0 383 452 1095
Size: 383 69 643
Fragments arranged by size:
643 383 69
NheI G'CTAG_C
Cuts at: 0 552 1095
Size: 552 543
PasI CC'CwG_GG
Cuts at: 0 961 1095
Size: 961 134
PpuMI rG'GwC_Cy
Cuts at: 0 288 426 1047 1095
Size: 288 138 621 48
Fragments arranged by size:
621 288 138 48
PspOMI G'GGCC_C
Cuts at: 0 4 28 432 715 955 1095
Size: 4 24 404 283 240 140
Fragments arranged by size:
404 283 240 140 24 4
PstI C_TGCA'G
Cuts at: 0 13 368 582 767 1095
Size: 13 355 214 185 328
Fragments arranged by size:
355 328 214 185 13
PvuII CAG'CTG
Cuts at: 0 349 828 1095
Size: 349 479 267
Fragments arranged by size:
479 349 267
SalI G'TCGA_C
Cuts at: 0 328 1095
Size: 328 767
SbfI CC_TGCA'GG
Cuts at: 0 582 1095
Size: 582 513
SfcI C'TryA_G
Cuts at: 0 9 364 578 763 1095
Size: 9 355 214 185 332
Fragments arranged by size:
355 332 214 185 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 462 648 1095
Size: 462 186 447
Fragments arranged by size:
462 447 186
StuI AGG'CCT
Cuts at: 0 642 1095
Size: 642 453
StyI C'CwwG_G
Cuts at: 0 128 284 308 422 739 875 993 1095
Size: 128 156 24 114 317 136 118 102
Fragments arranged by size:
317 156 136 128 118 114 102 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 501 903 1020 1095
Size: 501 402 117 75
Fragments arranged by size:
501 402 117 75
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 238 270 762 794 796 828 882 914
Size: 238 32 492 32 2 32 54 32
Cuts at: 914 1095
Size: 181
Fragments arranged by size:
492 238 181 54 32 32 32 32
2
XbaI T'CTAG_A
Cuts at: 0 978 1095
Size: 978 117
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 126 1095
Size: 126 969
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApaI AvaI AvrII
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI Bsp1286I BspMI BsrBI BsrFI BssSI BstXI
DraIII DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NgoMIV NheI PasI PpuMI PspOMI
PstI PvuII SalI SbfI SfcI SfiI StuI StyI
TaqII TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BpmI BpuEI BsaI BsaBI BsaWI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI EciI EcoICRI EcoRI EcoRV FspI FspAI
HindIII HpaI KasI MfeI MluI NarI NcoI NdeI
NotI NruI NsiI NspI PacI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI ScaI SexAI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TatI Tth111I XhoI XmaI XmnI ZraI