Restriction map for
pFN21AB7173
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BbeI BbrPI BbvCI BcgI
BfrI BglI BglII BlnI BsaAI
BsaBI BsaHI BsaI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstUI
BstZ17I CfoI Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AhdI 7 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 7 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1568 TGGCCA TGG^CCA
BamHI 515 GGATCC G^GATCC
BbsI 1411 GAAGAC GAAGAC(2/6)
BciVI 699 GTATCC GTATCC(6/5)
BclI 95 TGATCA T^GATCA
BcnI 750 CCCGG CC^[GC]GG
BfaI 1027 CTAG C^TAG
BpuAI 1411 GAAGAC GAAGAC(2/6)
BsgI 817 CTGCAC GTGCAG(16/14)
BspHI 338 TCATGA T^CATGA
BsrBI 473 GAGCGG CCGCTC(-3/-3)
BsrDI 323 GCAATG GCAATG(2/0)
BstEII 150 GGTGACC G^GT[ACGT]ACC
BstPI 150 GGTGACC G^GT[ACGT]ACC
DraI 826 TTTAAA TTT^AAA
DraIII 1114 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 7 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 150 GGTGACC G^GT[ACGT]ACC
FbaI 95 TGATCA T^GATCA
MaeI 1027 CTAG C^TAG
MluNI 1568 TGGCCA TGG^CCA
MscI 1568 TGGCCA TGG^CCA
NciI 750 CCCGG CC^[GC]GG
PflMI 498 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 479 AGGACCC [GA]G^G[AT]CC[CT]
PstI 10 CTGCAG CTGCA^G
PvuII 91 CAGCTG CAG^CTG
RcaI 338 TCATGA T^CATGA
SacI 307 GAGCTC GAGCT^C
ScaI 893 AGTACT AGT^ACT
SexAI 131 ACCTGGT A^CC[AT]GGT
SfcI 10 CTGCAG C^T[GA][CT]AG
SmlI 979 CTCAAG C^T[CT][GA]AG
TaqI 1328 TCGA T^CGA
TfiI 769 GAATC G^A[AT]TC
TthHB8I 1328 TCGA T^CGA
Van91I 498 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcsI 567 GAATTT [GA]^AATT[CT]
1148 AAATTT
AfaI 446 GTAC GT^AC
894 GTAC
AlwNI 235 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
499 CAGCTCCTG
ApaI 351 GGGCCC GGGCC^C
595 GGGCCC
ApoI 567 GAATTT [GA]^AATT[CT]
1148 AAATTT
AspHI 307 GAGCTC G[AT]GC[AT]^C
559 GTGCTC
BsaWI 1049 ACCGGA [AT]^CCGG[AT]
1499 ACCGGA
BseRI 438 CTCCTC GAGGAG(10/8)
614 CTCCTC
BsiHKAI 307 GAGCTC G[AT]GC[AT]^C
559 GTGCTC
BspMI 8 ACCTGC ACCTGC(4/8)
282 GCAGGT
BstYI 515 GGATCC [GA]^GATC[CT]
951 GGATCT
EarI 463 CTCTTC CTCTTC(1/4)
1453 GAAGAG
EcoNI 417 CCTGCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
462 CCTCTTCCAGG
Ksp632I 463 CTCTTC CTCTTC(1/4)
1453 GAAGAG
MflI 515 GGATCC [GA]^GATC[CT]
951 GGATCT
MspA1I 91 CAGCTG C[AC]G^C[GT]G
1254 CAGCGG
NcoI 29 CCATGG C^CATGG
1460 CCATGG
NspI 278 ACATGC [GA]CATG^[CT]
1541 ACATGC
RsaI 446 GTAC GT^AC
894 GTAC
StuI 107 AGGCCT AGG^CCT
1368 AGGCCT
Tsp509I 568 AATT ^AATT
1149 AATT
XhoII 515 GGATCC [GA]^GATC[CT]
951 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AocI AvaI
AvaII BanI BanII BbvI BlpI
BmyI BpmI Bpu1102I BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Bsu36I Cac8I CelII Cfr13I DdeI
DpnII DraII DsaI EaeI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MnlI MseI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
Sau3AI Sau96I ScrFI SfaNI StyI
Tru9I TseI Tsp45I TspRI