(Circular) (Six-base) MAPSORT of: pFN21AB7173.seq Check: 4003 from: 1 to: 6295 pFN21AB7173 6295 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2278 2278 Size: 6295 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2237 2888 3413 4358 5742 6010 1632 Size: 605 651 525 945 1384 268 1917 Fragments arranged by size: 1917 1384 945 651 605 525 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6098 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 5126 5126 Size: 6295 AhdI GACnn_n'nnGTC Cuts at: 2017 5024 2017 Size: 3007 3288 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2111 Size: 32 6263 AlwNI CAG_nnn'CTG Cuts at: 2245 2509 4038 5610 2245 Size: 264 1529 1572 2930 Fragments arranged by size: 2930 1572 1529 264 ApaI G_GGCC'C Cuts at: 2360 2604 2360 Size: 244 6051 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2572 3153 3604 3830 3982 6091 1493 Size: 1079 581 451 226 152 2109 1697 Fragments arranged by size: 2109 1697 1079 581 451 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 2252 2489 2597 3620 1870 Size: 73 309 237 108 1023 4545 Fragments arranged by size: 4545 1023 309 237 108 73 BamHI G'GATC_C Cuts at: 2520 3625 3992 2520 Size: 1105 367 4823 Fragments arranged by size: 4823 1105 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2698 2758 2837 3616 5071 Size: 325 217 627 911 60 79 779 1455 Cuts at: 5071 618 Size: 1842 Fragments arranged by size: 1842 1455 911 779 627 325 217 79 60 BanII G_rGCy'C Cuts at: 1391 2264 2316 2360 2544 2604 3483 3614 1391 Size: 873 52 44 184 60 879 131 4072 Fragments arranged by size: 4072 879 873 184 131 60 52 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3423 928 Size: 971 1524 3800 Fragments arranged by size: 3800 1524 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4486 4520 1903 Size: 34 2549 34 3678 Fragments arranged by size: 3678 2549 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2715 4187 5397 2715 Size: 1472 1210 3613 Fragments arranged by size: 3613 1472 1210 BclI T'GATC_A Cuts at: 1548 2100 1548 Size: 552 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 2094 2909 3239 3712 2094 Size: 815 330 473 4677 Fragments arranged by size: 4677 815 473 330 Bme1580I G_kGCm'C Cuts at: 1790 2360 2594 2604 2643 2979 4356 5512 1790 Size: 570 234 10 39 336 1377 1156 2573 Fragments arranged by size: 2573 1377 1156 570 336 234 39 10 BmgBI CAC'GTC Cuts at: 1318 2392 3174 1318 Size: 1074 782 4439 Fragments arranged by size: 4439 1074 782 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2258 3135 4026 Size: 416 53 83 399 24 841 877 891 Cuts at: 4026 4984 442 Size: 958 1753 Fragments arranged by size: 1753 958 891 877 841 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2317 2317 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2099 2402 2431 2552 2773 2870 4955 1239 Size: 860 303 29 121 221 97 2085 2579 Fragments arranged by size: 2579 2085 860 303 221 121 97 29 Bpu10I CC'TnA_GC Cuts at: 3112 3112 Size: 6295 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2969 4420 5285 5583 2969 Size: 1451 865 298 3681 Fragments arranged by size: 3681 1451 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 6289 6289 Size: 6295 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3054 3504 4728 5126 5400 5547 5958 3054 Size: 450 1224 398 274 147 411 3391 Fragments arranged by size: 3391 1224 450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2111 2141 2393 2423 3534 Size: 30 165 30 708 30 252 30 1111 Cuts at: 3534 3564 1178 Size: 30 3909 Fragments arranged by size: 3909 1111 708 252 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2434 2610 1186 Size: 1248 176 4871 Fragments arranged by size: 4871 1248 176 BseYI C'CCAG_C Cuts at: 2496 2502 2525 3187 3257 5498 5994 2496 Size: 6 23 662 70 2241 496 2797 Fragments arranged by size: 2797 2241 662 496 70 23 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2807 1163 Size: 573 1071 4651 Fragments arranged by size: 4651 1071 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2316 2568 4356 4441 5512 2316 Size: 252 1788 85 1071 3099 Fragments arranged by size: 3099 1788 1071 252 85 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 2316 2360 2544 2568 2594 2604 Size: 399 474 52 44 184 24 26 10 Cuts at: 2604 2643 2979 3483 3614 4356 4441 5512 1391 Size: 39 336 504 131 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 504 474 399 336 184 131 85 52 44 39 26 24 10 BspEI T'CCGG_A Cuts at: 5958 5958 Size: 6295 BspHI T'CATG_A Cuts at: 2343 4184 5914 2343 Size: 1841 1730 2724 Fragments arranged by size: 2724 1841 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2022 2278 3650 844 Size: 1178 256 1372 3489 Fragments arranged by size: 3489 1372 1178 256 BsrBI CCG'CTC Cuts at: 1394 1823 2480 4182 1394 Size: 429 657 1702 3507 Fragments arranged by size: 3507 1702 657 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 4790 4964 65 Size: 1183 1087 2455 174 1396 Fragments arranged by size: 2455 1396 1183 1087 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4939 5126 1700 Size: 110 3129 187 2869 Fragments arranged by size: 3129 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6295 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6295 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 2155 1184 Size: 971 5324 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2695 3148 2137 Size: 558 453 5284 Fragments arranged by size: 5284 558 453 BstYI r'GATC_y Cuts at: 1972 2520 2956 3625 3992 4377 4394 5846 5932 Size: 548 436 669 367 385 17 1452 86 Cuts at: 5932 5944 6290 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 669 548 436 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2516 2892 3485 2516 Size: 376 593 5326 Fragments arranged by size: 5326 593 376 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 2373 3465 513 Size: 552 939 30 339 1092 3343 Fragments arranged by size: 3343 1092 939 552 339 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 3223 3811 4605 4625 3223 Size: 588 794 20 4893 Fragments arranged by size: 4893 794 588 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6295 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 2833 3599 2833 Size: 766 5529 DraIII CAC_nnn'GTG Cuts at: 3124 3124 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5302 809 Size: 507 3986 1802 Fragments arranged by size: 3986 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 3312 3573 Size: 54 1425 50 275 18 396 1086 261 Cuts at: 3573 3742 4631 6260 8 Size: 169 889 1629 43 Fragments arranged by size: 1629 1425 1086 889 396 275 261 169 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2474 3453 4225 1353 Size: 1121 979 772 3423 Fragments arranged by size: 3423 1121 979 772 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2314 2314 Size: 6295 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2099 2237 2402 2431 2552 2773 2870 Size: 393 467 138 165 29 121 221 97 Cuts at: 2870 2888 3413 4358 4955 5742 6010 1239 Size: 18 525 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 525 467 393 268 221 165 138 121 97 29 18 EcoNI CCTnn'n_nnAGG Cuts at: 2426 2471 2426 Size: 45 6250 EcoO109I rG'GnC_Cy Cuts at: 2149 2356 2357 2434 2485 2601 2950 3184 2149 Size: 207 1 77 51 116 349 234 5260 Fragments arranged by size: 5260 349 234 207 116 77 51 1 EcoRI G'AATT_C Cuts at: 3604 3982 3604 Size: 378 5917 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2917 2917 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 3187 3219 4936 Size: 32 135 32 431 32 1152 32 1717 Cuts at: 4936 4968 5010 5042 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1717 1152 431 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6295 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 3894 3894 Size: 6295 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5409 5593 1305 Size: 4104 184 2007 Fragments arranged by size: 4104 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3575 10 Size: 54 1425 343 1743 2730 Fragments arranged by size: 2730 1743 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2039 2282 2288 2756 2930 3125 3290 Size: 648 873 243 6 468 174 195 165 Cuts at: 3290 4253 4612 4771 518 Size: 963 359 159 2042 Fragments arranged by size: 2042 963 873 648 468 359 243 195 174 165 159 6 MspA1I CmG'CkG Cuts at: 1606 2098 3261 3711 4388 5536 5781 1606 Size: 492 1163 450 677 1148 245 2120 Fragments arranged by size: 2120 1163 1148 677 492 450 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2034 3465 513 Size: 552 939 30 1431 3343 Fragments arranged by size: 3343 1431 939 552 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 2287 3550 3653 5198 1132 Size: 97 1058 1263 103 1545 2229 Fragments arranged by size: 2229 1545 1263 1058 103 97 PasI CC'CwG_GG Cuts at: 1348 2353 2587 1348 Size: 1005 234 5056 Fragments arranged by size: 5056 1005 234 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 2509 1108 Size: 1401 4894 PfoI T'CCnGG_A Cuts at: 2508 3015 2508 Size: 507 5788 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 4364 4396 5877 5909 2111 Size: 32 2221 32 1481 32 2497 Fragments arranged by size: 2497 2221 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2485 2485 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 3865 3865 Size: 6295 PspOMI G'GGCC_C Cuts at: 2356 2600 2356 Size: 244 6051 PstI C_TGCA'G Cuts at: 830 2019 3647 830 Size: 1189 1628 3478 Fragments arranged by size: 3478 1628 1189 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2098 2098 Size: 6295 SacI G_AGCT'C Cuts at: 2316 2316 Size: 6295 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 2900 4543 6255 2900 Size: 1643 1712 2940 Fragments arranged by size: 2940 1712 1643 SexAI A'CCwGG_T Cuts at: 2136 2136 Size: 6295 SfcI C'TryA_G Cuts at: 826 1045 2015 3643 4778 5459 5650 826 Size: 219 970 1628 1135 681 191 1471 Fragments arranged by size: 1628 1471 1135 970 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2984 4399 5300 5562 820 Size: 197 926 1041 1415 901 262 1553 Fragments arranged by size: 1553 1415 1041 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 2114 3375 1517 Size: 597 1261 4437 Fragments arranged by size: 4437 1261 597 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2061 3315 3451 3465 3734 Size: 552 939 30 27 1254 136 14 269 Cuts at: 3734 513 Size: 3074 Fragments arranged by size: 3074 1254 939 552 269 136 30 27 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2366 2855 4322 4339 4492 4677 5122 Size: 1255 786 489 1467 17 153 185 445 Cuts at: 5122 325 Size: 1498 Fragments arranged by size: 1498 1467 1255 786 489 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2898 4541 6253 Size: 274 80 33 51 1443 921 1643 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 1712 1643 1443 921 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2393 2425 3044 3076 3568 3600 6255 6287 2393 Size: 32 619 32 492 32 2655 32 2401 Fragments arranged by size: 2655 2401 619 492 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6295 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 4424 4424 Size: 6295 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI