Restriction map for
pFN21AB7172
##################################
# NO CUT ENZYMES ( 126 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglII BlnI BsaAI BsaHI
BsiEI BsiWI BsmI BsoBI Bsp1407I
BspDI BspLU11I BspMI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
Bsu36I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoRV FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HpaI KasI KpnI KspI
MfeI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccI 814 GTAGAC GT^[AC][GT]AC
AccII 681 CGCG CG^CG
AccIII 500 TCCGGA T^CCGGA
AspHI 328 GAGCTC G[AT]GC[AT]^C
AspI 775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 599 TGGCCA TGG^CCA
BanI 940 GGTGCC G^G[CT][GA]CC
BanII 328 GAGCTC G[GA]GC[CT]^C
BbsI 1420 GAAGAC GAAGAC(2/6)
BfaI 937 CTAG C^TAG
BglI 802 GCCACGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 550 GCTAAGC GC^T[ACGT]AGC
BmyI 328 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 550 GCTAAGC GC^T[ACGT]AGC
BpuAI 1420 GAAGAC GAAGAC(2/6)
BsaBI 208 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 932 GGTCTC GGTCTC(1/5)
BseAI 500 TCCGGA T^CCGGA
BsgI 1269 GTGCAG GTGCAG(16/14)
BsiHKAI 328 GAGCTC G[AT]GC[AT]^C
BsmBI 446 CGTCTC CGTCTC(1/5)
BsmFI 398 GGGAC GGGAC(10/14)
Bsp1286I 328 GAGCTC G[AGT]GC[ACT]^C
BspEI 500 TCCGGA T^CCGGA
BspHI 1296 TCATGA T^CATGA
BsrBI 728 CCGCTC CCGCTC(-3/-3)
BsrDI 687 CATTGC GCAATG(2/0)
BssSI 385 CACGAG CACGAG(-5/-1)
BstEII 1134 GGTCACC G^GT[ACGT]ACC
BstPI 1134 GGTCACC G^GT[ACGT]ACC
BstUI 681 CGCG CG^CG
BstYI 869 AGATCC [GA]^GATC[CT]
CelII 550 GCTAAGC GC^T[ACGT]AGC
EcoO65I 1134 GGTCACC G^GT[ACGT]ACC
EcoRI 896 GAATTC G^AATTC
EcoT22I 1167 ATGCAT ATGCA^T
FauI 1144 GCGGG CCCGC(4/6)
MaeI 937 CTAG C^TAG
MaeII 805 ACGT A^CGT
MamI 208 GATCGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 869 AGATCC [GA]^GATC[CT]
MluNI 599 TGGCCA TGG^CCA
MroI 500 TCCGGA T^CCGGA
MscI 599 TGGCCA TGG^CCA
MspA1I 1336 CAGCGG C[AC]G^C[GT]G
MvnI 681 CGCG CG^CG
NsiI 1167 ATGCAT ATGCA^T
PflFI 775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1073 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 926 GACTTCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 348 CTGCAG CTGCA^G
RcaI 1296 TCATGA T^CATGA
SacI 328 GAGCTC GAGCT^C
SapI 330 GCTCTTC GCTCTTC(1/4)
SmlI 573 CTTGAG C^T[CT][GA]AG
TaqI 308 TCGA T^CGA
Tsp45I 1135 GTCAC ^GT[GC]AC
Tth111I 775 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 308 TCGA T^CGA
Van91I 1073 CCATCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 630 CCAGAATGAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 869 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 13 enzymes) #
###################################
BpmI 160 CTGGAG CTGGAG(16/14)
466 CTGGAG
BsaWI 500 TCCGGA [AT]^CCGG[AT]
657 TCCGGT
DdeI 551 CTAAG C^T[ACGT]AG
949 CTGAG
DsaI 995 CCGTGG C^C[GA][CT]GG
1031 CCGTGG
EaeI 185 TGGCCG [CT]^GGCC[GA]
599 TGGCCA
HincII 49 GTCAAC GT[CT]^[GA]AC
82 GTTGAC
HindII 49 GTCAAC GT[CT]^[GA]AC
82 GTTGAC
HindIII 553 AAGCTT A^AGCTT
904 AAGCTT
MaeIII 1135 GTCAC ^GT[ACGT]AC
1439 GTAAC
MseI 71 TTAA T^TAA
1313 TTAA
NlaIV 398 GGGACC GG[ACGT]^[ACGT]CC
940 GGTGCC
ScaI 311 AGTACT AGT^ACT
1232 AGTACT
Tru9I 71 TTAA T^TAA
1313 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BbvI BcnI BsaJI
BseRI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DpnII EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MboI
MboII MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TfiI TseI
Tsp509I TspRI