(Circular) (Six-base) MAPSORT of: pFN21AB7172.seq Check: 8502 from: 1 to: 6178 pFN21AB7172 6178 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3988 278 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2820 3521 1937 Size: 883 701 4594 Fragments arranged by size: 4594 883 701 Acc65I G'GTAC_C Cuts at: 3499 3499 Size: 6178 AclI AA'CG_TT Cuts at: 1563 4305 4678 1563 Size: 2742 373 3063 Fragments arranged by size: 3063 2742 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2172 4241 5625 5893 1632 Size: 540 2069 1384 268 1917 Fragments arranged by size: 2069 1917 1384 540 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5981 AflIII A'CryG_T Cuts at: 1203 5077 6054 1203 Size: 3874 977 1327 Fragments arranged by size: 3874 1327 977 AgeI A'CCGG_T Cuts at: 5009 5009 Size: 6178 AhdI GACnn_n'nnGTC Cuts at: 4907 4907 Size: 6178 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6178 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2120 1184 Size: 936 5242 AlwNI CAG_nnn'CTG Cuts at: 3921 5493 3921 Size: 1572 4606 ApaLI G'TGCA_C Cuts at: 4235 5391 4235 Size: 1156 5022 ApoI r'AATT_y Cuts at: 1493 2245 2750 2901 3487 3713 3865 5974 1493 Size: 752 505 151 586 226 152 2109 1697 Fragments arranged by size: 2109 1697 752 586 505 226 152 151 AseI AT'TA_AT Cuts at: 160 1032 4732 160 Size: 872 3700 1606 Fragments arranged by size: 3700 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 AvaI C'yCGr_G Cuts at: 1870 1943 3503 1870 Size: 73 1560 4545 Fragments arranged by size: 4545 1560 73 BamHI G'GATC_C Cuts at: 3508 3875 3508 Size: 367 5811 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2945 3499 4954 618 Size: 325 217 627 1158 554 1455 1842 Fragments arranged by size: 1842 1455 1158 627 554 325 217 BanII G_rGCy'C Cuts at: 1391 2337 3497 1391 Size: 946 1160 4072 Fragments arranged by size: 4072 1160 946 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3432 928 Size: 971 1533 3674 Fragments arranged by size: 3674 1533 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4369 4403 1903 Size: 34 2432 34 3678 Fragments arranged by size: 3678 2432 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4070 5280 4070 Size: 1210 4968 BclI T'GATC_A Cuts at: 1548 1548 Size: 6178 BfrBI ATG'CAT Cuts at: 3174 3174 Size: 6178 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2813 4789 136 Size: 107 122 71 631 605 1141 1976 1525 Fragments arranged by size: 1976 1525 1141 631 605 122 107 71 BglII A'GATC_T Cuts at: 6173 6173 Size: 6178 BlpI GC'TnA_GC Cuts at: 2556 3595 2556 Size: 1039 5139 Bme1580I G_kGCm'C Cuts at: 1790 4239 5395 1790 Size: 2449 1156 2573 Fragments arranged by size: 2573 2449 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6178 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2135 2329 2410 Size: 416 53 83 399 24 718 194 81 Cuts at: 2410 3909 4867 442 Size: 1499 958 1753 Fragments arranged by size: 1753 1499 958 718 416 399 194 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 2492 4838 1239 Size: 947 306 2346 2579 Fragments arranged by size: 2579 2346 947 306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2599 4303 5168 5466 2599 Size: 1704 865 298 3311 Fragments arranged by size: 3311 1704 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2943 4841 882 Size: 617 1444 1898 2219 Fragments arranged by size: 2219 1898 1444 617 BsaAI yAC'GTr Cuts at: 493 1204 3969 493 Size: 711 2765 2702 Fragments arranged by size: 2765 2702 711 BsaBI GATnn'nnATC Cuts at: 2217 6172 2217 Size: 3955 2223 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3985 4367 5002 275 Size: 53 83 186 936 2452 382 635 1451 Fragments arranged by size: 2452 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 2662 4611 5009 5283 5430 5841 2505 Size: 157 1949 398 274 147 411 2842 Fragments arranged by size: 2842 1949 411 398 274 157 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 2330 2672 2702 Size: 30 165 30 867 30 30 342 30 Cuts at: 2702 3437 3467 1178 Size: 735 30 3889 Fragments arranged by size: 3889 867 735 342 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2294 2425 2563 1186 Size: 1108 131 138 4801 Fragments arranged by size: 4801 1108 138 131 BseYI C'CCAG_C Cuts at: 5381 5877 5381 Size: 496 5682 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3295 1163 Size: 573 1559 4046 Fragments arranged by size: 4046 1559 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3628 4389 5417 1540 Size: 275 186 1627 761 1028 2301 Fragments arranged by size: 2301 1627 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2337 4239 4324 5395 2337 Size: 1902 85 1071 3120 Fragments arranged by size: 3120 1902 1071 85 BsmI GAATG_Cn' Cuts at: 3689 3782 3689 Size: 93 6085 BsmBI CGTCTCn'nnnn_ Cuts at: 2457 2457 Size: 6178 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 3497 4239 4324 5395 1391 Size: 399 547 1160 742 85 1071 2174 Fragments arranged by size: 2174 1160 1071 742 547 399 85 BspEI T'CCGG_A Cuts at: 2505 5841 2505 Size: 3336 2842 BspHI T'CATG_A Cuts at: 3301 4067 5797 3301 Size: 766 1730 3682 Fragments arranged by size: 3682 1730 766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3533 844 Size: 2689 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2735 4065 1394 Size: 429 912 1330 3507 Fragments arranged by size: 3507 1330 912 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 4673 4847 65 Size: 1183 1443 1982 174 1396 Fragments arranged by size: 1982 1443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4822 5009 1700 Size: 110 3012 187 2869 Fragments arranged by size: 3012 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6178 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6178 BssSI C'ACGA_G Cuts at: 2390 4238 5250 2390 Size: 1848 1012 3318 Fragments arranged by size: 3318 1848 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6178 BstBI TT'CG_AA Cuts at: 4982 4982 Size: 6178 BstEII G'GTnAC_C Cuts at: 1184 3139 1184 Size: 1955 4223 BstXI CCAn_nnnn'nTGG Cuts at: 2033 2975 3457 2033 Size: 942 482 4754 Fragments arranged by size: 4754 942 482 BstYI r'GATC_y Cuts at: 1972 2874 3508 3875 4260 4277 5729 5815 5827 Size: 902 634 367 385 17 1452 86 12 Cuts at: 5827 6173 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 902 634 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3000 3036 513 Size: 552 939 996 36 3655 Fragments arranged by size: 3655 996 939 552 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2012 3145 487 Size: 683 56 786 1133 3520 Fragments arranged by size: 3520 1133 786 683 56 BtsI GCAGTG_nn' Cuts at: 2486 3283 3694 4488 4508 2486 Size: 797 411 794 20 4156 Fragments arranged by size: 4156 797 794 411 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6178 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6143 DraI TTT'AAA Cuts at: 3482 3482 Size: 6178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5185 809 Size: 507 3869 1802 Fragments arranged by size: 3869 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2190 2604 3625 Size: 54 1425 50 275 18 360 414 1021 Cuts at: 3625 4514 6143 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1021 889 414 360 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3625 1537 Size: 275 1813 4090 Fragments arranged by size: 4090 1813 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2148 2342 2439 2829 2996 3376 4108 1353 Size: 795 194 97 390 167 380 732 3423 Fragments arranged by size: 3423 795 732 390 380 194 167 97 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4765 5139 5285 387 Size: 1064 138 3176 374 146 1280 Fragments arranged by size: 3176 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2335 2335 Size: 6178 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2172 2186 2492 4241 4838 5625 5893 Size: 393 540 14 306 1749 597 787 268 Cuts at: 5893 1239 Size: 1524 Fragments arranged by size: 1749 1524 787 597 540 393 306 268 14 EcoRI G'AATT_C Cuts at: 2901 3487 3865 2901 Size: 586 378 5214 Fragments arranged by size: 5214 586 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2996 2996 Size: 6178 FspI TGC'GCA Cuts at: 4684 4684 Size: 6178 HaeII r_GCGC'y Cuts at: 1678 5325 1678 Size: 3647 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2667 2699 2771 2803 3041 Size: 32 135 32 1095 32 72 32 238 Cuts at: 3041 3073 3386 3418 4819 4851 4893 4925 1373 Size: 32 313 32 1401 32 42 32 2626 Fragments arranged by size: 2626 1401 1095 313 238 135 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2056 2089 3522 3768 669 Size: 988 281 118 33 1433 246 3079 Fragments arranged by size: 3079 1433 988 281 246 118 33 HindIII A'AGCT_T Cuts at: 748 2558 2909 748 Size: 1810 351 4017 Fragments arranged by size: 4017 1810 351 HpaI GTT'AAC Cuts at: 3768 3768 Size: 6178 KpnI G_GTAC'C Cuts at: 3503 3503 Size: 6178 MfeI C'AATT_G Cuts at: 3777 3777 Size: 6178 MluI A'CGCG_T Cuts at: 6054 6054 Size: 6178 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 5292 5476 1305 Size: 1302 2685 184 2007 Fragments arranged by size: 2685 2007 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2606 10 Size: 54 1425 343 774 3582 Fragments arranged by size: 3582 1425 774 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3104 3191 3263 3386 4136 4495 4654 Size: 648 1938 87 72 123 750 359 159 Cuts at: 4654 518 Size: 2042 Fragments arranged by size: 2042 1938 750 648 359 159 123 87 72 MspA1I CmG'CkG Cuts at: 1606 3343 3594 4271 5419 5664 1606 Size: 1737 251 677 1148 245 2120 Fragments arranged by size: 2120 1737 1148 677 251 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4687 Fragments arranged by size: 4687 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6178 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6178 NotI GC'GGCC_GC Cuts at: 3625 3625 Size: 6178 NsiI A_TGCA'T Cuts at: 3176 3176 Size: 6178 NspI r_CATG'y Cuts at: 1132 1229 2273 2626 3174 3536 5081 1132 Size: 97 1044 353 548 362 1545 2229 Fragments arranged by size: 2229 1545 1044 548 362 353 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6178 PciI A'CATG_T Cuts at: 5077 5077 Size: 6178 PflMI CCAn_nnn'nTGG Cuts at: 1108 3084 1108 Size: 1976 4202 PmeI GTTT'AAAC Cuts at: 3482 3482 Size: 6178 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4247 4279 5760 5792 4247 Size: 32 1481 32 4633 Fragments arranged by size: 4633 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2935 1613 Size: 1322 4856 PsiI TTA'TAA Cuts at: 3748 3748 Size: 6178 PstI C_TGCA'G Cuts at: 830 2357 3530 830 Size: 1527 1173 3478 Fragments arranged by size: 3478 1527 1173 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6178 SacI G_AGCT'C Cuts at: 2337 2337 Size: 6178 SalI G'TCGA_C Cuts at: 1936 3520 1936 Size: 1584 4594 SapI GCTCTTCn'nnn_ Cuts at: 2342 2342 Size: 6178 SbfI CC_TGCA'GG Cuts at: 3530 3530 Size: 6178 ScaI AGT'ACT Cuts at: 2318 3239 4426 6138 2318 Size: 921 1187 1712 2358 Fragments arranged by size: 2358 1712 1187 921 SfcI C'TryA_G Cuts at: 826 1045 2353 2817 3375 3526 4661 5342 5533 Size: 219 1308 464 558 151 1135 681 191 Cuts at: 5533 826 Size: 1471 Fragments arranged by size: 1471 1308 1135 681 558 464 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6178 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6178 SmaI CCC'GGG Cuts at: 1872 3505 1872 Size: 1633 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2578 4282 5183 5445 820 Size: 197 926 635 1704 901 262 1553 Fragments arranged by size: 1704 1553 926 901 635 262 197 SnaBI TAC'GTA Cuts at: 493 3969 493 Size: 3476 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6178 SphI G_CATG'C Cuts at: 1132 3536 1132 Size: 2404 3774 SspI AAT'ATT Cuts at: 5 52 4102 5 Size: 47 4050 2081 Fragments arranged by size: 4050 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6178 StyI C'CwwG_G Cuts at: 513 1065 2004 2826 3144 3456 3617 513 Size: 552 939 822 318 312 161 3074 Fragments arranged by size: 3074 939 822 552 318 312 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2925 3146 4205 4222 4375 4560 5005 Size: 1255 1345 221 1059 17 153 185 445 Cuts at: 5005 325 Size: 1498 Fragments arranged by size: 1498 1345 1255 1059 445 221 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2316 3237 4424 Size: 274 80 33 51 1443 339 921 1187 Cuts at: 4424 6136 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1187 921 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 2672 2704 6138 6170 2270 Size: 32 370 32 3434 32 2278 Fragments arranged by size: 3434 2278 370 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2783 1316 Size: 1467 4711 XbaI T'CTAG_A Cuts at: 3514 3514 Size: 6178 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2642 2642 Size: 6178 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6178 XmaI C'CCGG_G Cuts at: 1870 3503 1870 Size: 1633 4545 XmnI GAAnn'nnTTC Cuts at: 4307 4307 Size: 6178 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3986 276 Size: 53 83 186 936 2452 2468 Fragments arranged by size: 2468 2452 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI