(Circular) (Six-base) MAPSORT of: pFN21AB7169.seq Check: 3919 from: 1 to: 6004 pFN21AB7169 6004 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2711 4067 5451 5719 1632 Size: 1079 1356 1384 268 1917 Fragments arranged by size: 1917 1384 1356 1079 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 4903 5880 1203 Size: 3700 977 1327 Fragments arranged by size: 3700 1327 977 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6004 AlwNI CAG_nnn'CTG Cuts at: 2076 2734 3747 5319 2076 Size: 658 1013 1572 2761 Fragments arranged by size: 2761 1572 1013 658 ApaLI G'TGCA_C Cuts at: 2294 4061 5217 2294 Size: 1767 1156 3081 Fragments arranged by size: 3081 1767 1156 ApoI r'AATT_y Cuts at: 1493 3313 3539 3691 5800 1493 Size: 1820 226 152 2109 1697 Fragments arranged by size: 2109 1820 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2943 3035 3329 1870 Size: 73 1000 92 294 4545 Fragments arranged by size: 4545 1000 294 92 73 BamHI G'GATC_C Cuts at: 2103 2532 2697 3334 3701 2103 Size: 429 165 637 367 4406 Fragments arranged by size: 4406 637 429 367 165 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3323 1391 Size: 1932 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3209 928 Size: 971 1310 3723 Fragments arranged by size: 3723 1310 971 BbvCI CC'TCA_GC Cuts at: 2447 2447 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2685 4615 136 Size: 107 122 71 631 605 1013 1930 1525 Fragments arranged by size: 1930 1525 1013 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3260 3421 3260 Size: 161 5843 Bme1580I G_kGCm'C Cuts at: 1790 2289 2298 2989 4065 5221 1790 Size: 499 9 691 1076 1156 2573 Fragments arranged by size: 2573 1156 1076 691 499 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6004 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3735 4693 442 Size: 416 53 83 399 24 2318 958 1753 Fragments arranged by size: 2318 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2096 2096 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2035 2453 4664 1239 Size: 796 418 2211 2579 Fragments arranged by size: 2579 2211 796 418 Bpu10I CC'TnA_GC Cuts at: 2447 2976 3251 2447 Size: 529 275 5200 Fragments arranged by size: 5200 529 275 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2861 3116 4667 882 Size: 617 1362 255 1551 2219 Fragments arranged by size: 2219 1551 1362 617 255 BsaAI yAC'GTr Cuts at: 493 1204 2281 3795 493 Size: 711 1077 1514 2702 Fragments arranged by size: 2702 1514 1077 711 BsaBI GATnn'nnATC Cuts at: 2108 5998 2108 Size: 3890 2114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2100 2529 2700 4437 4835 5109 5256 5667 2100 Size: 429 171 1737 398 274 147 411 2437 Fragments arranged by size: 2437 1737 429 411 398 274 171 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3161 3191 1178 Size: 30 165 30 1758 30 3991 Fragments arranged by size: 3991 1758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2327 2354 2767 2921 3035 3095 1186 Size: 868 273 27 413 154 114 60 4095 Fragments arranged by size: 4095 868 413 273 154 114 60 27 BseYI C'CCAG_C Cuts at: 2172 2344 5207 5703 2172 Size: 172 2863 496 2473 Fragments arranged by size: 2863 2473 496 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5431 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2124 3454 4215 5243 1540 Size: 275 186 123 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 123 BsiHKAI G_wGCw'C Cuts at: 2055 2298 4065 4150 5221 2055 Size: 243 1767 85 1071 2838 Fragments arranged by size: 2838 1767 1071 243 85 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 2263 2263 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2289 2298 2989 3323 4065 4150 Size: 399 265 234 9 691 334 742 85 Cuts at: 4150 5221 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 691 399 334 265 234 85 9 BspEI T'CCGG_A Cuts at: 2100 2529 5667 2100 Size: 429 3138 2437 Fragments arranged by size: 3138 2437 429 BspHI T'CATG_A Cuts at: 3893 5623 3893 Size: 1730 4274 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3359 844 Size: 2515 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3891 1394 Size: 429 2068 3507 Fragments arranged by size: 3507 2068 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2766 4499 4673 65 Size: 1183 1518 1733 174 1396 Fragments arranged by size: 1733 1518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2556 4648 4835 1700 Size: 110 746 2092 187 2869 Fragments arranged by size: 2869 2092 746 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2293 3190 794 Size: 1499 897 3608 Fragments arranged by size: 3608 1499 897 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2180 2578 2180 Size: 398 5606 BstYI r'GATC_y Cuts at: 1972 2103 2532 2697 3334 3701 4086 4103 5555 Size: 131 429 165 637 367 385 17 1452 Cuts at: 5555 5641 5653 5999 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 637 429 385 367 346 165 131 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2601 3075 513 Size: 552 939 597 474 3442 Fragments arranged by size: 3442 939 597 552 474 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5265 Fragments arranged by size: 5265 683 56 BtsI GCAGTG_nn' Cuts at: 2876 2960 3520 4314 4334 2876 Size: 84 560 794 20 4546 Fragments arranged by size: 4546 794 560 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5969 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DraIII CAC_nnn'GTG Cuts at: 2293 2293 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5011 809 Size: 507 3695 1802 Fragments arranged by size: 3695 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 3096 3451 Size: 54 1425 50 275 18 291 975 355 Cuts at: 3451 4340 5969 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 975 889 355 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2121 3451 1537 Size: 275 309 1330 4090 Fragments arranged by size: 4090 1330 309 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2390 2708 2811 3150 3934 1353 Size: 1037 318 103 339 784 3423 Fragments arranged by size: 3423 1037 784 339 318 103 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2351 4591 4965 5111 387 Size: 1064 138 762 2240 374 146 1280 Fragments arranged by size: 2240 1280 1064 762 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2453 2711 4067 4664 5451 5719 Size: 393 403 418 258 1356 597 787 268 Cuts at: 5719 1239 Size: 1524 Fragments arranged by size: 1524 1356 787 597 418 403 393 268 258 EcoNI CCTnn'n_nnAGG Cuts at: 2713 2713 Size: 6004 EcoO109I rG'GnC_Cy Cuts at: 2439 2451 2924 2439 Size: 12 473 5519 Fragments arranged by size: 5519 473 12 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 5151 1678 Size: 3473 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2524 2556 3149 Size: 32 135 32 563 32 357 32 593 Cuts at: 3149 3161 3181 3193 4645 4677 4719 4751 1373 Size: 12 20 12 1452 32 42 32 2626 Fragments arranged by size: 2626 1452 593 563 357 135 42 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 5880 5880 Size: 6004 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2178 3147 5118 5302 1305 Size: 873 969 1971 184 2007 Fragments arranged by size: 2007 1971 969 873 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4182 Fragments arranged by size: 4182 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3962 4321 4480 518 Size: 648 2796 359 159 2042 Fragments arranged by size: 2796 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2403 2550 3197 3420 4097 5245 5490 1606 Size: 797 147 647 223 677 1148 245 2120 Fragments arranged by size: 2120 1148 797 677 647 245 223 147 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4513 Fragments arranged by size: 4513 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3362 4907 1132 Size: 97 2133 1545 2229 Fragments arranged by size: 2229 2133 1545 97 PasI CC'CwG_GG Cuts at: 1348 2734 2929 1348 Size: 1386 195 4423 Fragments arranged by size: 4423 1386 195 PciI A'CATG_T Cuts at: 4903 4903 Size: 6004 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2439 2451 2924 2439 Size: 12 473 5519 Fragments arranged by size: 5519 473 12 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2183 2215 2183 Size: 32 5972 PstI C_TGCA'G Cuts at: 830 2247 2555 3019 3356 830 Size: 1417 308 464 337 3478 Fragments arranged by size: 3478 1417 464 337 308 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 3197 3197 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SanDI GG'GwC_CC Cuts at: 2439 2439 Size: 6004 SapI GCTCTTCn'nnn_ Cuts at: 2390 3150 2390 Size: 760 5244 SbfI CC_TGCA'GG Cuts at: 3356 3356 Size: 6004 ScaI AGT'ACT Cuts at: 4252 5964 4252 Size: 1712 4292 SfcI C'TryA_G Cuts at: 826 1045 2243 2551 2721 3015 3352 4487 5168 Size: 219 1198 308 170 294 337 1135 681 Cuts at: 5168 5359 826 Size: 191 1471 Fragments arranged by size: 1471 1198 1135 681 337 308 294 219 191 170 SfiI GGCCn_nnn'nGGCC Cuts at: 2685 2685 Size: 6004 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2945 3331 1872 Size: 1073 386 4545 Fragments arranged by size: 4545 1073 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 2210 2719 1517 Size: 693 509 4802 Fragments arranged by size: 4802 693 509 StyI C'CwwG_G Cuts at: 513 1065 2004 2113 2793 3443 513 Size: 552 939 109 680 650 3074 Fragments arranged by size: 3074 939 680 650 552 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2186 2227 2864 4031 4048 4201 4386 Size: 1255 606 41 637 1167 17 153 185 Cuts at: 4386 4831 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1167 637 606 445 185 153 41 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4250 5962 96 Size: 274 80 33 51 1443 2273 1712 138 Fragments arranged by size: 2273 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6004 XbaI T'CTAG_A Cuts at: 3340 3340 Size: 6004 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2943 3329 1870 Size: 1073 386 4545 Fragments arranged by size: 4545 1073 386 XmnI GAAnn'nnTTC Cuts at: 4133 4133 Size: 6004 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SexAI SfoI SrfI SwaI XcmI