(Circular) (Six-base) MAPSORT of: pFN21AB7168.seq Check: 6789 from: 1 to: 5941 pFN21AB7168 5941 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2826 3284 1937 Size: 889 458 4594 Fragments arranged by size: 4594 889 458 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2602 4004 5388 5656 1632 Size: 970 1402 1384 268 1917 Fragments arranged by size: 1917 1402 1384 970 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2919 2951 2919 Size: 32 5909 AlwNI CAG_nnn'CTG Cuts at: 3684 5256 3684 Size: 1572 4369 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2143 3026 3193 3250 3476 3628 5737 1493 Size: 650 883 167 57 226 152 2109 1697 Fragments arranged by size: 2109 1697 883 650 226 167 152 57 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 3266 1870 Size: 73 1323 4545 Fragments arranged by size: 4545 1323 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4717 618 Size: 325 217 627 1475 1455 1842 Fragments arranged by size: 1842 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3260 1391 Size: 1869 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2250 2608 2742 928 Size: 971 351 358 134 4127 Fragments arranged by size: 4127 971 358 351 134 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 2215 2486 3833 5043 2002 Size: 213 271 1347 1210 2900 Fragments arranged by size: 2900 1347 1210 271 213 BclI T'GATC_A Cuts at: 1548 2253 1548 Size: 705 5236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2630 4552 136 Size: 107 122 71 631 605 958 1922 1525 Fragments arranged by size: 1922 1525 958 631 605 122 107 71 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 4002 5158 1790 Size: 2212 1156 2573 Fragments arranged by size: 2573 2212 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5941 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2481 2895 3672 Size: 416 53 83 399 24 1064 414 777 Cuts at: 3672 4630 442 Size: 958 1753 Fragments arranged by size: 1753 1064 958 777 416 414 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2005 2977 3208 4601 1239 Size: 766 972 231 1393 2579 Fragments arranged by size: 2579 1393 972 766 231 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 2417 5935 2417 Size: 3518 2423 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4374 4772 5046 5193 5604 4374 Size: 398 274 147 411 4711 Fragments arranged by size: 4711 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2215 2245 1178 Size: 30 165 30 812 30 4874 Fragments arranged by size: 4874 812 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5941 BseYI C'CCAG_C Cuts at: 2097 2576 5144 5640 2097 Size: 479 2568 496 2398 Fragments arranged by size: 2568 2398 496 479 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2847 3086 1163 Size: 573 1111 239 4018 Fragments arranged by size: 4018 1111 573 239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4002 4087 5158 4002 Size: 85 1071 4785 Fragments arranged by size: 4785 1071 85 BsmI GAATG_Cn' Cuts at: 2996 3452 3545 2996 Size: 456 93 5392 Fragments arranged by size: 5392 456 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3260 4002 4087 5158 1391 Size: 399 1470 742 85 1071 2174 Fragments arranged by size: 2174 1470 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 2712 3830 5560 2712 Size: 1118 1730 3093 Fragments arranged by size: 3093 1730 1118 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3296 844 Size: 2452 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2533 2587 2967 4436 4610 65 Size: 1183 1285 54 380 1469 174 1396 Fragments arranged by size: 1469 1396 1285 1183 380 174 54 BsrFI r'CCGG_y Cuts at: 1700 1810 4585 4772 1700 Size: 110 2775 187 2869 Fragments arranged by size: 2869 2775 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5941 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 3095 4001 5013 3095 Size: 906 1012 4023 Fragments arranged by size: 4023 1012 906 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2333 2831 3271 3638 4023 4040 5492 5578 Size: 361 498 440 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 498 440 385 367 361 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2827 2827 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2004 2217 513 Size: 552 939 213 4237 Fragments arranged by size: 4237 939 552 213 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2121 487 Size: 683 56 895 4307 Fragments arranged by size: 4307 895 683 56 BtsI GCAGTG_nn' Cuts at: 2805 3457 4251 4271 2805 Size: 652 794 20 4475 Fragments arranged by size: 4475 794 652 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2595 2595 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3388 4277 5906 Size: 54 1425 50 275 18 1558 889 1629 Cuts at: 5906 8 Size: 43 Fragments arranged by size: 1629 1558 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 3871 1353 Size: 1160 1358 3423 Fragments arranged by size: 3423 1358 1160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2005 2602 2977 3208 4004 4601 5388 Size: 393 373 597 375 231 796 597 787 Cuts at: 5388 5656 1239 Size: 268 1524 Fragments arranged by size: 1524 796 787 597 597 393 375 373 268 231 EcoO109I rG'GnC_Cy Cuts at: 2473 2473 Size: 5941 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2490 2522 2794 2826 3126 Size: 32 135 32 918 32 272 32 300 Cuts at: 3126 3158 4582 4614 4656 4688 1373 Size: 32 1424 32 42 32 2626 Fragments arranged by size: 2626 1424 918 300 272 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2668 3285 3531 669 Size: 988 281 730 617 246 3079 Fragments arranged by size: 3079 988 730 617 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2861 5055 5239 1305 Size: 1556 2194 184 2007 Fragments arranged by size: 2194 2007 1556 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4119 Fragments arranged by size: 4119 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2069 2352 2786 3899 4258 4417 518 Size: 648 903 283 434 1113 359 159 2042 Fragments arranged by size: 2042 1113 903 648 434 359 283 159 MspA1I CmG'CkG Cuts at: 1606 3357 4034 5182 5427 1606 Size: 1751 677 1148 245 2120 Fragments arranged by size: 2120 1751 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2217 513 Size: 552 939 213 4237 Fragments arranged by size: 4237 939 552 213 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2857 3299 4844 1132 Size: 97 1628 442 1545 2229 Fragments arranged by size: 2229 1628 1545 442 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 2945 1108 Size: 1487 350 4104 Fragments arranged by size: 4104 1487 350 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2206 2238 2206 Size: 32 5909 PstI C_TGCA'G Cuts at: 830 2995 3293 830 Size: 2165 298 3478 Fragments arranged by size: 3478 2165 298 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2610 2676 2991 3289 4424 5105 5296 Size: 219 1565 66 315 298 1135 681 191 Cuts at: 5296 826 Size: 1471 Fragments arranged by size: 1565 1471 1135 681 315 298 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 3268 1872 Size: 1396 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2217 3018 3380 513 Size: 552 939 213 801 362 3074 Fragments arranged by size: 3074 939 801 552 362 213 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 3968 3985 4138 4323 4768 325 Size: 1255 796 1592 17 153 185 445 1498 Fragments arranged by size: 1592 1498 1255 796 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3052 4187 5899 Size: 274 80 33 51 1443 1075 1135 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 1712 1443 1135 1075 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 2404 3277 2404 Size: 873 5068 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2788 2068 Size: 720 5221 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 3266 1870 Size: 1396 4545 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI