(Circular) (Six-base) MAPSORT of: pFN21AB7166.seq Check: 155 from: 1 to: 5893 pFN21AB7166 5893 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3703 278 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3236 1937 Size: 1299 4594 Acc65I G'GTAC_C Cuts at: 3214 3214 Size: 5893 AclI AA'CG_TT Cuts at: 1563 4020 4393 1563 Size: 2457 373 3063 Fragments arranged by size: 3063 2457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3956 5340 5608 1632 Size: 2324 1384 268 1917 Fragments arranged by size: 2324 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5696 AflIII A'CryG_T Cuts at: 1203 4792 5769 1203 Size: 3589 977 1327 Fragments arranged by size: 3589 1327 977 AgeI A'CCGG_T Cuts at: 4724 4724 Size: 5893 AhdI GACnn_n'nnGTC Cuts at: 4622 4622 Size: 5893 AleI CACnn'nnGTG Cuts at: 1166 2060 1166 Size: 894 4999 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5893 AlwNI CAG_nnn'CTG Cuts at: 2548 3636 5208 2548 Size: 1088 1572 3233 Fragments arranged by size: 3233 1572 1088 ApaLI G'TGCA_C Cuts at: 3950 5106 3950 Size: 1156 4737 ApoI r'AATT_y Cuts at: 1493 2153 3202 3428 3580 5689 1493 Size: 660 1049 226 152 2109 1697 Fragments arranged by size: 2109 1697 1049 660 226 152 AseI AT'TA_AT Cuts at: 160 1032 4447 160 Size: 872 3415 1606 Fragments arranged by size: 3415 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 AvaI C'yCGr_G Cuts at: 1870 1943 3099 3218 1870 Size: 73 1156 119 4545 Fragments arranged by size: 4545 1156 119 73 BamHI G'GATC_C Cuts at: 2413 2815 3223 3590 2413 Size: 402 408 367 4716 Fragments arranged by size: 4716 408 402 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3214 4669 618 Size: 325 217 627 1427 1455 1842 Fragments arranged by size: 1842 1455 1427 627 325 217 BanII G_rGCy'C Cuts at: 1391 2383 2523 3212 1391 Size: 992 140 689 4072 Fragments arranged by size: 4072 992 689 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2086 3182 928 Size: 971 187 1096 3639 Fragments arranged by size: 3639 1096 971 187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4084 4118 1903 Size: 34 2147 34 3678 Fragments arranged by size: 3678 2147 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2436 3785 4995 2436 Size: 1349 1210 3334 Fragments arranged by size: 3334 1349 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5893 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4504 136 Size: 107 122 71 631 605 2832 1525 Fragments arranged by size: 2832 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2070 5888 2070 Size: 3818 2075 BlpI GC'TnA_GC Cuts at: 3310 3310 Size: 5893 Bme1580I G_kGCm'C Cuts at: 1790 3954 5110 1790 Size: 2164 1156 2573 Fragments arranged by size: 2573 2164 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5893 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3624 4582 442 Size: 416 53 83 399 24 2207 958 1753 Fragments arranged by size: 2207 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5893 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3029 4553 1239 Size: 1790 1524 2579 Fragments arranged by size: 2579 1790 1524 Bpu10I CC'TnA_GC Cuts at: 2484 3039 2484 Size: 555 5338 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2831 4018 4883 5181 2831 Size: 1187 865 298 3543 Fragments arranged by size: 3543 1187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4556 882 Size: 617 3057 2219 Fragments arranged by size: 3057 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3684 493 Size: 711 2480 2702 Fragments arranged by size: 2702 2480 711 BsaBI GATnn'nnATC Cuts at: 5887 5887 Size: 5893 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3700 4082 4717 275 Size: 53 83 186 936 2167 382 635 1451 Fragments arranged by size: 2167 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2097 4326 4724 4998 5145 5556 2097 Size: 2229 398 274 147 411 2434 Fragments arranged by size: 2434 2229 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5668 Fragments arranged by size: 5668 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2051 2136 2411 3002 1186 Size: 865 85 275 591 4077 Fragments arranged by size: 4077 865 591 275 85 BseYI C'CCAG_C Cuts at: 2998 3131 5096 5592 2998 Size: 133 1965 496 3299 Fragments arranged by size: 3299 1965 496 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2650 1163 Size: 573 914 4406 Fragments arranged by size: 4406 914 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3343 4104 5132 1540 Size: 275 186 1342 761 1028 2301 Fragments arranged by size: 2301 1342 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2316 3954 4039 5110 2316 Size: 1638 85 1071 3099 Fragments arranged by size: 3099 1638 1071 85 BsmI GAATG_Cn' Cuts at: 3404 3497 3404 Size: 93 5800 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2316 2383 2523 3212 3954 4039 5110 Size: 399 526 67 140 689 742 85 1071 Cuts at: 5110 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 689 526 399 140 85 67 BspEI T'CCGG_A Cuts at: 2097 5556 2097 Size: 3459 2434 BspHI T'CATG_A Cuts at: 2523 3782 5512 2523 Size: 1259 1730 2904 Fragments arranged by size: 2904 1730 1259 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3248 844 Size: 2404 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3780 1394 Size: 429 1957 3507 Fragments arranged by size: 3507 1957 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2364 2877 4388 4562 65 Size: 1183 1116 513 1511 174 1396 Fragments arranged by size: 1511 1396 1183 1116 513 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4537 4724 1700 Size: 110 2727 187 2869 Fragments arranged by size: 2869 2727 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5893 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5893 BssSI C'ACGA_G Cuts at: 3953 4965 3953 Size: 1012 4881 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5893 BstBI TT'CG_AA Cuts at: 4697 4697 Size: 5893 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5893 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 5893 BstYI r'GATC_y Cuts at: 1972 2070 2413 2815 3052 3223 3590 3975 3992 Size: 98 343 402 237 171 367 385 17 Cuts at: 3992 5444 5530 5542 5888 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 402 385 367 346 343 237 171 98 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5341 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5154 Fragments arranged by size: 5154 683 56 BtsI GCAGTG_nn' Cuts at: 3147 3409 4203 4223 3147 Size: 262 794 20 4817 Fragments arranged by size: 4817 794 262 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5893 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5858 DraI TTT'AAA Cuts at: 3197 3197 Size: 5893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2643 4900 809 Size: 507 1327 2257 1802 Fragments arranged by size: 2257 1802 1327 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2494 3340 4229 Size: 54 1425 50 275 18 664 846 889 Cuts at: 4229 5858 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 846 664 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3340 1537 Size: 275 1528 4090 Fragments arranged by size: 4090 1528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2403 3823 1353 Size: 1050 1420 3423 Fragments arranged by size: 3423 1420 1050 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4480 4854 5000 387 Size: 1064 138 2891 374 146 1280 Fragments arranged by size: 2891 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3029 3956 4553 5340 5608 1239 Size: 393 1397 927 597 787 268 1524 Fragments arranged by size: 1524 1397 927 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2033 2033 Size: 5893 EcoRI G'AATT_C Cuts at: 3202 3580 3202 Size: 378 5515 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2710 2710 Size: 5893 FspI TGC'GCA Cuts at: 4399 4399 Size: 5893 HaeII r_GCGC'y Cuts at: 1678 5040 1678 Size: 3362 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2115 2147 4534 4566 4608 Size: 32 135 32 543 32 2387 32 42 Cuts at: 4608 4640 1373 Size: 32 2626 Fragments arranged by size: 2626 2387 543 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3237 3483 669 Size: 988 281 1299 246 3079 Fragments arranged by size: 3079 1299 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5893 HpaI GTT'AAC Cuts at: 3483 3483 Size: 5893 KpnI G_GTAC'C Cuts at: 3218 3218 Size: 5893 MfeI C'AATT_G Cuts at: 3492 3492 Size: 5893 MluI A'CGCG_T Cuts at: 5769 5769 Size: 5893 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1991 3162 5007 5191 1305 Size: 686 1171 1845 184 2007 Fragments arranged by size: 2007 1845 1171 686 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4071 Fragments arranged by size: 4071 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 2252 3851 4210 4369 518 Size: 648 894 192 1599 359 159 2042 Fragments arranged by size: 2042 1599 894 648 359 192 159 MspA1I CmG'CkG Cuts at: 1606 2114 3002 3022 3309 3986 5134 5379 1606 Size: 508 888 20 287 677 1148 245 2120 Fragments arranged by size: 2120 1148 888 677 508 287 245 20 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5341 NdeI CA'TA_TG Cuts at: 387 387 Size: 5893 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5893 NotI GC'GGCC_GC Cuts at: 3340 3340 Size: 5893 NspI r_CATG'y Cuts at: 1132 1229 3251 4796 1132 Size: 97 2022 1545 2229 Fragments arranged by size: 2229 2022 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5893 PciI A'CATG_T Cuts at: 4792 4792 Size: 5893 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 4991 PmeI GTTT'AAAC Cuts at: 3197 3197 Size: 5893 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3962 3994 5475 5507 3962 Size: 32 1481 32 4348 Fragments arranged by size: 4348 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3182 1613 Size: 1569 4324 PsiI TTA'TAA Cuts at: 2829 3463 2829 Size: 634 5259 PstI C_TGCA'G Cuts at: 830 2544 3021 3245 830 Size: 1714 477 224 3478 Fragments arranged by size: 3478 1714 477 224 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5893 PvuII CAG'CTG Cuts at: 2114 3002 2114 Size: 888 5005 SalI G'TCGA_C Cuts at: 1936 3235 1936 Size: 1299 4594 SbfI CC_TGCA'GG Cuts at: 3245 3245 Size: 5893 ScaI AGT'ACT Cuts at: 4141 5853 4141 Size: 1712 4181 SfcI C'TryA_G Cuts at: 826 1045 2115 2540 3017 3070 3241 4376 5057 Size: 219 1070 425 477 53 171 1135 681 Cuts at: 5057 5248 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1070 681 477 425 219 191 171 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5893 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5893 SmaI CCC'GGG Cuts at: 1872 3101 3220 1872 Size: 1229 119 4545 Fragments arranged by size: 4545 1229 119 SmlI C'TyrA_G Cuts at: 820 1017 1943 2810 3997 4898 5160 820 Size: 197 926 867 1187 901 262 1553 Fragments arranged by size: 1553 1187 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 3684 493 Size: 3191 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5893 SphI G_CATG'C Cuts at: 1132 3251 1132 Size: 2119 3774 SspI AAT'ATT Cuts at: 5 52 3817 5 Size: 47 3765 2081 Fragments arranged by size: 3765 2081 47 StuI AGG'CCT Cuts at: 1517 2724 3068 1517 Size: 1207 344 4342 Fragments arranged by size: 4342 1207 344 StyI C'CwwG_G Cuts at: 513 1065 3332 513 Size: 552 2267 3074 Fragments arranged by size: 3074 2267 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2224 3920 3937 4090 4275 4720 325 Size: 1255 644 1696 17 153 185 445 1498 Fragments arranged by size: 1696 1498 1255 644 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 4139 5851 Size: 274 80 33 51 1443 927 1235 1712 Cuts at: 5851 96 Size: 138 Fragments arranged by size: 1712 1443 1235 927 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2036 2068 5853 5885 2036 Size: 32 3785 32 2044 Fragments arranged by size: 3785 2044 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5893 XbaI T'CTAG_A Cuts at: 2672 3229 2672 Size: 557 5336 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5893 XmaI C'CCGG_G Cuts at: 1870 3099 3218 1870 Size: 1229 119 4545 Fragments arranged by size: 4545 1229 119 XmnI GAAnn'nnTTC Cuts at: 4022 4022 Size: 5893 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3701 276 Size: 53 83 186 936 2167 2468 Fragments arranged by size: 2468 2167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI