Restriction map for
pFN21AB7162
##################################
# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI Alw44I AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvrII BalI
BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaBI BsgI BsiWI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HaeII HindIII
HinfI HpaI KasI KpnI MaeI
MamI MfeI MluI MluNI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 425 TCCGGA T^CCGGA
AflIII 764 ACGTGT A^C[GA][CT]GT
AhdI 283 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 705 GATCC GGATC(4/5)
ApaI 1025 GGGCCC GGGCC^C
AspEI 283 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 779 TGCGCA TGC^GCA
BbrPI 763 CACGTG CAC^GTG
BlpI 720 GCTCAGC GC^T[ACGT]AGC
BpmI 960 CTCCAG CTGGAG(16/14)
Bpu1102I 720 GCTCAGC GC^T[ACGT]AGC
BsaWI 425 TCCGGA [AT]^CCGG[AT]
BseAI 425 TCCGGA T^CCGGA
BspEI 425 TCCGGA T^CCGGA
BsrFI 967 ACCGGC [GA]^CCGG[CT]
BssSI 505 CACGAG CACGAG(-5/-1)
BstAPI 264 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 704 AGATCC [GA]^GATC[CT]
CelII 720 GCTCAGC GC^T[ACGT]AGC
Cfr10I 967 ACCGGC [GA]^CCGG[CT]
DdeI 721 CTCAG C^T[ACGT]AG
DraIII 535 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 283 GACAACTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 956 CCTGCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 779 TGCGCA TGC^GCA
HincII 1050 GTTGAC GT[CT]^[GA]AC
HindII 1050 GTTGAC GT[CT]^[GA]AC
KspI 930 CCGCGG CCGC^GG
MaeIII 115 GTTAC ^GT[ACGT]AC
MflI 704 AGATCC [GA]^GATC[CT]
MroI 425 TCCGGA T^CCGGA
NlaIII 569 CATG CATG^
PmaCI 763 CACGTG CAC^GTG
PmlI 763 CACGTG CAC^GTG
PpuMI 914 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 798 CAGCTG CAG^CTG
SacII 930 CCGCGG CCGC^GG
SapI 687 GAAGAGC GCTCTTC(1/4)
TspRI 42 CACTG CA[GC]TG[ACGT][ACGT]^
XcmI 1109 CCACGGGCAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 704 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 448 GGCGTC G[GA]^CG[CT]C
636 GGCGTC
AvaI 1003 CCCGAG C^[CT]CG[GA]G
1042 CCCGAG
BanI 679 GGCACC G^G[CT][GA]CC
997 GGTGCC
BanII 1025 GGGCCC G[GA]GC[CT]^C
1039 GAGCCC
BsaAI 268 TACGTG [CT]AC^GT[GA]
763 CACGTG
BsaHI 448 GGCGTC G[GA]^CG[CT]C
636 GGCGTC
BsaI 901 GAGACC GGTCTC(1/5)
290 GGTCTC
BsiEI 440 CGGCCG CG[GA][CT]^CG
969 CGGCCG
BsmAI 901 GAGAC GTCTC(1/5)
291 GTCTC
BsmFI 914 GGGAC GGGAC(10/14)
153 GTCCC
BsoBI 1003 CCCGAG C^[CT]CG[GA]G
1042 CCCGAG
BsrI 287 ACTGG ACTGG(1/-1)
420 CCAGT
BssHII 576 GCGCGC G^CGCGC
657 GCGCGC
DpnII 177 GATC ^GATC
705 GATC
EagI 440 CGGCCG C^GGCCG
969 CGGCCG
EclXI 440 CGGCCG C^GGCCG
969 CGGCCG
Eco52I 440 CGGCCG C^GGCCG
969 CGGCCG
EcoT14I 470 CCAAGG C^C[AT][AT]GG
812 CCAAGG
Hin1I 448 GGCGTC G[GA]^CG[CT]C
636 GGCGTC
MaeII 269 ACGT A^CGT
764 ACGT
MboI 177 GATC ^GATC
705 GATC
MslI 348 CATCAGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
372 CATCTCCGTG
NdeII 177 GATC ^GATC
705 GATC
PflMI 90 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
708 CCAGCGGCTGG
Sau3AI 177 GATC ^GATC
705 GATC
SfiI 936 GGCCTGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
975 GGCCAGGGAGGCC
StuI 983 AGGCCT AGG^CCT
1007 AGGCCT
StyI 470 CCAAGG C^C[AT][AT]GG
812 CCAAGG
Van91I 90 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
708 CCAGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AspHI AvaII BbvI BcnI BglI
BmyI BsaJI BseRI BsiHKAI BsiYI
BslI Bsp1286I BspMI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DraII DsaI EaeI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HpaII
HphI ItaI Ksp632I MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIV PstI RsaI Sau96I
ScrFI SfaNI SfcI TaqI TseI
TthHB8I