Restriction map for
pFN21AB7162
(Circular) (Six-base) MAPSORT of: pFN21AB7162.seq Check: 7596 from: 1 to: 5830
pFN21AB7162 5830 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2969 2969
Size: 5830
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3640 278
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3173 1937
Size: 1236 4594
Acc65I G'GTAC_C
Cuts at: 3151 3151
Size: 5830
AclI AA'CG_TT
Cuts at: 1563 3957 4330 1563
Size: 2394 373 3063
Fragments arranged by size:
3063 2394 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2007 2459 3893 5277 5545 1632
Size: 375 452 1434 1384 268 1917
Fragments arranged by size:
1917 1434 1384 452 375 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5633
AflIII A'CryG_T
Cuts at: 1203 2769 4729 5706 1203
Size: 1566 1960 977 1327
Fragments arranged by size:
1960 1566 1327 977
AgeI A'CCGG_T
Cuts at: 4661 4661
Size: 5830
AhdI GACnn_n'nnGTC
Cuts at: 2293 4559 2293
Size: 2266 3564
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5830
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5830
AlwNI CAG_nnn'CTG
Cuts at: 2101 2719 2793 3573 5145 2101
Size: 618 74 780 1572 2786
Fragments arranged by size:
2786 1572 780 618 74
ApaI G_GGCC'C
Cuts at: 3034 3034
Size: 5830
ApaLI G'TGCA_C
Cuts at: 3887 5043 3887
Size: 1156 4674
ApoI r'AATT_y
Cuts at: 1493 3139 3365 3517 5626 1493
Size: 1646 226 152 2109 1697
Fragments arranged by size:
2109 1697 1646 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4384 160
Size: 872 3352 1606
Fragments arranged by size:
3352 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
AvaI C'yCGr_G
Cuts at: 1870 1943 3008 3047 3155 1870
Size: 73 1065 39 108 4545
Fragments arranged by size:
4545 1065 108 73 39
BamHI G'GATC_C
Cuts at: 3160 3527 3160
Size: 367 5463
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2684 3002 3151 4606 618
Size: 325 217 627 897 318 149 1455 1842
Fragments arranged by size:
1842 1455 897 627 325 318 217 149
BanII G_rGCy'C
Cuts at: 1391 3034 3048 3149 1391
Size: 1643 14 101 4072
Fragments arranged by size:
4072 1643 101 14
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4859
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4021 4055 1903
Size: 34 2084 34 3678
Fragments arranged by size:
3678 2084 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3722 4932 3722
Size: 1210 4620
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5830
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2263 2948 2987
Size: 107 122 71 631 605 591 685 39
Cuts at: 2987 3011 4441 136
Size: 24 1430 1525
Fragments arranged by size:
1525 1430 685 631 605 591 122 107
71 39 24
BglII A'GATC_T
Cuts at: 5825 5825
Size: 5830
BlpI GC'TnA_GC
Cuts at: 2726 3247 2726
Size: 521 5309
Bme1580I G_kGCm'C
Cuts at: 1790 3007 3034 3891 5047 1790
Size: 1217 27 857 1156 2573
Fragments arranged by size:
2573 1217 1156 857 27
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5830
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3561 4519 442
Size: 416 53 83 399 24 2144 958 1753
Fragments arranged by size:
2144 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5830
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2950 4490 1239
Size: 1711 1540 2579
Fragments arranged by size:
2579 1711 1540
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3955 4820 5118 3955
Size: 865 298 4667
Fragments arranged by size:
4667 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2301 2900 4493 882
Size: 617 802 599 1593 2219
Fragments arranged by size:
2219 1593 802 617 599
BsaAI yAC'GTr
Cuts at: 493 1204 2275 2770 3621 493
Size: 711 1071 495 851 2702
Fragments arranged by size:
2702 1071 851 711 495
BsaBI GATnn'nnATC
Cuts at: 5824 5824
Size: 5830
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2454 2642 3637 4019
Size: 53 83 186 936 921 188 995 382
Cuts at: 4019 4654 275
Size: 635 1451
Fragments arranged by size:
1451 995 936 921 635 382 188 186
83 53
BsaWI w'CCGG_w
Cuts at: 2430 4263 4661 4935 5082 5493 2430
Size: 1833 398 274 147 411 2767
Fragments arranged by size:
2767 1833 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2828 2858 1178
Size: 30 165 30 1425 30 4150
Fragments arranged by size:
4150 1425 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2050 2528 3056 1186
Size: 837 27 478 528 3960
Fragments arranged by size:
3960 837 528 478 27
BseYI C'CCAG_C
Cuts at: 2200 2998 5033 5529 2200
Size: 798 2035 496 2501
Fragments arranged by size:
2501 2035 798 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5257
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2448 2977 3280 4041 5069 1540
Size: 275 186 447 529 303 761 1028 2301
Fragments arranged by size:
2301 1028 761 529 447 303 275 186
BsiHKAI G_wGCw'C
Cuts at: 2559 2727 2889 3891 3976 5047 2559
Size: 168 162 1002 85 1071 3342
Fragments arranged by size:
3342 1071 1002 168 162 85
BsmI GAATG_Cn'
Cuts at: 3341 3434 3341
Size: 93 5737
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2559 2727 2889 3007 3034 3048 3149
Size: 399 769 168 162 118 27 14 101
Cuts at: 3149 3891 3976 5047 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 769 742 399 168 162 118
101 85 27 14
BspEI T'CCGG_A
Cuts at: 2430 5493 2430
Size: 3063 2767
BspHI T'CATG_A
Cuts at: 3719 5449 3719
Size: 1730 4100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2184 2969 3111 3185 844
Size: 1340 785 142 74 3489
Fragments arranged by size:
3489 1340 785 142 74
BsrBI CCG'CTC
Cuts at: 1394 1823 3717 1394
Size: 429 1894 3507
Fragments arranged by size:
3507 1894 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4325 4499 65
Size: 1183 3077 174 1396
Fragments arranged by size:
3077 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2972 4474 4661 1700
Size: 110 1162 1502 187 2869
Fragments arranged by size:
2869 1502 1162 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5830
BssHII G'CGCG_C
Cuts at: 1925 2581 2662 1925
Size: 656 81 5093
Fragments arranged by size:
5093 656 81
BssSI C'ACGA_G
Cuts at: 2510 3890 4902 2510
Size: 1380 1012 3438
Fragments arranged by size:
3438 1380 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2275 794
Size: 1481 4349
BstBI TT'CG_AA
Cuts at: 4634 4634
Size: 5830
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5830
BstYI r'GATC_y
Cuts at: 1972 2709 3160 3527 3912 3929 5381 5467 5479
Size: 737 451 367 385 17 1452 86 12
Cuts at: 5479 5825 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 737 451 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2382 2680 2733 2935 3114 513
Size: 552 1317 298 53 202 179 3229
Fragments arranged by size:
3229 1317 552 298 202 179 53
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5091
Fragments arranged by size:
5091 683 56
BtsI GCAGTG_nn'
Cuts at: 2046 3346 4140 4160 2046
Size: 1300 794 20 3716
Fragments arranged by size:
3716 1300 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5830
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5795
DraI TTT'AAA
Cuts at: 3134 3134
Size: 5830
DraIII CAC_nnn'GTG
Cuts at: 2545 2545
Size: 5830
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4837 809
Size: 507 3521 1802
Fragments arranged by size:
3521 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2229 2445
Size: 54 1425 50 275 18 315 84 216
Cuts at: 2445 2974 3126 3277 4166 5795 8
Size: 529 152 151 889 1629 43
Fragments arranged by size:
1629 1425 889 529 315 275 216 152
151 84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2445 2974 3277 1537
Size: 275 633 529 303 4090
Fragments arranged by size:
4090 633 529 303 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2243 2624 2687 3760 1353
Size: 890 381 63 1073 3423
Fragments arranged by size:
3423 1073 890 381 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4417 4791 4937 387
Size: 1064 138 2828 374 146 1280
Fragments arranged by size:
2828 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2007 2459 2950 3893 4490 5277 5545
Size: 393 375 452 491 943 597 787 268
Cuts at: 5545 1239
Size: 1524
Fragments arranged by size:
1524 943 787 597 491 452 393 375
268
EcoNI CCTnn'n_nnAGG
Cuts at: 2965 2965
Size: 5830
EcoO109I rG'GnC_Cy
Cuts at: 2920 2940 3030 2920
Size: 20 90 5720
Fragments arranged by size:
5720 90 20
EcoRI G'AATT_C
Cuts at: 3139 3517 3139
Size: 378 5452
FspI TGC'GCA
Cuts at: 2786 4336 2786
Size: 1550 4280
HaeII r_GCGC'y
Cuts at: 1678 4977 1678
Size: 3299 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2045 2077 2279 2311 4471
Size: 32 135 32 473 32 202 32 2160
Cuts at: 4471 4503 4545 4577 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2160 473 202 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3057 3174 3420 669
Size: 988 281 1119 117 246 3079
Fragments arranged by size:
3079 1119 988 281 246 117
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5830
HpaI GTT'AAC
Cuts at: 3420 3420
Size: 5830
KpnI G_GTAC'C
Cuts at: 3155 3155
Size: 5830
MfeI C'AATT_G
Cuts at: 3429 3429
Size: 5830
MluI A'CGCG_T
Cuts at: 5706 5706
Size: 5830
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4944 5128 1305
Size: 3639 184 2007
Fragments arranged by size:
3639 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4008
Fragments arranged by size:
4008 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2381 3788 4147 4306 518
Size: 648 1191 24 1407 359 159 2042
Fragments arranged by size:
2042 1407 1191 648 359 159 24
MspA1I CmG'CkG
Cuts at: 1606 2716 2805 2937 2998 3246 3923 5071 5316
Size: 1110 89 132 61 248 677 1148 245
Cuts at: 5316 1606
Size: 2120
Fragments arranged by size:
2120 1148 1110 677 248 245 132 89
61
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5278
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5830
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5830
NotI GC'GGCC_GC
Cuts at: 3277 3277
Size: 5830
NspI r_CATG'y
Cuts at: 1132 1229 3188 4733 1132
Size: 97 1959 1545 2229
Fragments arranged by size:
2229 1959 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3034 1348
Size: 1686 4144
PciI A'CATG_T
Cuts at: 4729 4729
Size: 5830
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2101 2719 1108
Size: 993 618 4219
Fragments arranged by size:
4219 993 618
PfoI T'CCnGG_A
Cuts at: 2966 3017 2966
Size: 51 5779
PmeI GTTT'AAAC
Cuts at: 3134 3134
Size: 5830
PmlI CAC'GTG
Cuts at: 2770 2770
Size: 5830
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3899 3931 5412 5444 3899
Size: 32 1481 32 4285
Fragments arranged by size:
4285 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2920 2920
Size: 5830
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5830
PsiI TTA'TAA
Cuts at: 3400 3400
Size: 5830
PspOMI G'GGCC_C
Cuts at: 3030 3030
Size: 5830
PstI C_TGCA'G
Cuts at: 830 2053 2181 2271 2283 3182 830
Size: 1223 128 90 12 899 3478
Fragments arranged by size:
3478 1223 899 128 90 12
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5830
PvuII CAG'CTG
Cuts at: 2805 2805
Size: 5830
SacII CC_GC'GG
Cuts at: 2938 2938
Size: 5830
SalI G'TCGA_C
Cuts at: 1936 3172 1936
Size: 1236 4594
SapI GCTCTTCn'nnn_
Cuts at: 2687 2687
Size: 5830
SbfI CC_TGCA'GG
Cuts at: 3182 3182
Size: 5830
ScaI AGT'ACT
Cuts at: 4078 5790 4078
Size: 1712 4118
SfcI C'TryA_G
Cuts at: 826 1045 2049 2177 2267 2279 3178 4313 4994
Size: 219 1004 128 90 12 899 1135 681
Cuts at: 4994 5185 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1004 899 681 219 191 128
90 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 2948 2987 2948
Size: 39 5791
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5830
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5830
SmaI CCC'GGG
Cuts at: 1872 3157 1872
Size: 1285 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3934 4835 5097 820
Size: 197 926 1991 901 262 1553
Fragments arranged by size:
1991 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3621 493
Size: 3128 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5830
SphI G_CATG'C
Cuts at: 1132 3188 1132
Size: 2056 3774
SspI AAT'ATT
Cuts at: 5 52 3754 5
Size: 47 3702 2081
Fragments arranged by size:
3702 2081 47
StuI AGG'CCT
Cuts at: 1517 2990 3014 1517
Size: 1473 24 4333
Fragments arranged by size:
4333 1473 24
StyI C'CwwG_G
Cuts at: 513 1065 2475 2817 3269 513
Size: 552 1410 342 452 3074
Fragments arranged by size:
3074 1410 552 452 342
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 2927 2990 3857 3874 4027 4212
Size: 1255 1122 225 63 867 17 153 185
Cuts at: 4212 4657 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1122 867 445 225 185 153
63 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4076 5788 96
Size: 274 80 33 51 1443 2099 1712 138
Fragments arranged by size:
2099 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5790 5822 5790
Size: 32 5798
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5830
XbaI T'CTAG_A
Cuts at: 3166 3166
Size: 5830
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 3121 2011
Size: 1110 4720
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5830
XmaI C'CCGG_G
Cuts at: 1870 3155 1870
Size: 1285 4545
XmnI GAAnn'nnTTC
Cuts at: 3959 3959
Size: 5830
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3638 276
Size: 53 83 186 936 2104 2468
Fragments arranged by size:
2468 2104 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PspXI PsrI RsrII SacI SanDI
SexAI SfoI SrfI SwaI