Restriction map for
pFN21AB7162
(Linear) (Six-base) MAPSORT of: KIBB7162.seq Check: 5386 from: 1 to: 1125
KIBB7162.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 964 1125
Size: 964 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 454 1125
Size: 2 452 671
Fragments arranged by size:
671 452 2
AflIII A'CryG_T
Cuts at: 0 764 1125
Size: 764 361
AhdI GACnn_n'nnGTC
Cuts at: 0 288 1125
Size: 288 837
AlwNI CAG_nnn'CTG
Cuts at: 0 96 714 788 1125
Size: 96 618 74 337
Fragments arranged by size:
618 337 96 74
ApaI G_GGCC'C
Cuts at: 0 1029 1125
Size: 1029 96
AvaI C'yCGr_G
Cuts at: 0 1003 1042 1125
Size: 1003 39 83
Fragments arranged by size:
1003 83 39
BanI G'GyrC_C
Cuts at: 0 679 997 1125
Size: 679 318 128
Fragments arranged by size:
679 318 128
BanII G_rGCy'C
Cuts at: 0 1029 1043 1125
Size: 1029 14 82
Fragments arranged by size:
1029 82 14
BglI GCCn_nnn'nGGC
Cuts at: 0 258 943 982 1006 1125
Size: 258 685 39 24 119
Fragments arranged by size:
685 258 119 39 24
BlpI GC'TnA_GC
Cuts at: 0 721 1125
Size: 721 404
Bme1580I G_kGCm'C
Cuts at: 0 1002 1029 1125
Size: 1002 27 96
Fragments arranged by size:
1002 96 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 945 1125
Size: 945 180
BsaI GGTCTCn'nnnn_
Cuts at: 0 296 895 1125
Size: 296 599 230
Fragments arranged by size:
599 296 230
BsaAI yAC'GTr
Cuts at: 0 270 765 1125
Size: 270 495 360
Fragments arranged by size:
495 360 270
BsaHI Gr'CG_yC
Cuts at: 0 449 637 1125
Size: 449 188 488
Fragments arranged by size:
488 449 188
BsaWI w'CCGG_w
Cuts at: 0 425 1125
Size: 425 700
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1125
Size: 823 30 272
Fragments arranged by size:
823 272 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 45 523 1051 1125
Size: 18 27 478 528 74
Fragments arranged by size:
528 478 74 27 18
BseYI C'CCAG_C
Cuts at: 0 195 993 1125
Size: 195 798 132
Fragments arranged by size:
798 195 132
BsiEI CG_ry'CG
Cuts at: 0 443 972 1125
Size: 443 529 153
Fragments arranged by size:
529 443 153
BsiHKAI G_wGCw'C
Cuts at: 0 554 722 884 1125
Size: 554 168 162 241
Fragments arranged by size:
554 241 168 162
Bsp1286I G_dGCh'C
Cuts at: 0 554 722 884 1002 1029 1043 1125
Size: 554 168 162 118 27 14 82
Fragments arranged by size:
554 168 162 118 82 27 14
BspEI T'CCGG_A
Cuts at: 0 425 1125
Size: 425 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 964 1106 1125
Size: 179 785 142 19
Fragments arranged by size:
785 179 142 19
BsrFI r'CCGG_y
Cuts at: 0 967 1125
Size: 967 158
BssHII G'CGCG_C
Cuts at: 0 576 657 1125
Size: 576 81 468
Fragments arranged by size:
576 468 81
BssSI C'ACGA_G
Cuts at: 0 505 1125
Size: 505 620
BstAPI GCAn_nnn'nTGC
Cuts at: 0 270 1125
Size: 270 855
BstYI r'GATC_y
Cuts at: 0 704 1125
Size: 704 421
BtgI C'CryG_G
Cuts at: 0 377 675 728 930 1109 1125
Size: 377 298 53 202 179 16
Fragments arranged by size:
377 298 202 179 53 16
BtsI GCAGTG_nn'
Cuts at: 0 41 1125
Size: 41 1084
DraIII CAC_nnn'GTG
Cuts at: 0 540 1125
Size: 540 585
EaeI y'GGCC_r
Cuts at: 0 140 224 440 969 1125
Size: 140 84 216 529 156
Fragments arranged by size:
529 216 156 140 84
EagI C'GGCC_G
Cuts at: 0 440 969 1125
Size: 440 529 156
Fragments arranged by size:
529 440 156
EarI CTCTTCn'nnn_
Cuts at: 0 238 619 682 1125
Size: 238 381 63 443
Fragments arranged by size:
443 381 238 63
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 454 945 1125
Size: 2 452 491 180
Fragments arranged by size:
491 452 180 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 960 1125
Size: 960 165
EcoO109I rG'GnC_Cy
Cuts at: 0 915 935 1025 1125
Size: 915 20 90 100
Fragments arranged by size:
915 100 90 20
FspI TGC'GCA
Cuts at: 0 781 1125
Size: 781 344
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 274 306 1125
Size: 40 32 202 32 819
Fragments arranged by size:
819 202 40 32 32
HincII GTy'rAC
Cuts at: 0 1052 1125
Size: 1052 73
MslI CAynn'nnrTG
Cuts at: 0 352 376 1125
Size: 352 24 749
Fragments arranged by size:
749 352 24
MspA1I CmG'CkG
Cuts at: 0 711 800 932 993 1125
Size: 711 89 132 61 132
Fragments arranged by size:
711 132 132 89 61
PasI CC'CwG_GG
Cuts at: 0 1029 1125
Size: 1029 96
PflMI CCAn_nnn'nTGG
Cuts at: 0 96 714 1125
Size: 96 618 411
Fragments arranged by size:
618 411 96
PfoI T'CCnGG_A
Cuts at: 0 961 1012 1125
Size: 961 51 113
Fragments arranged by size:
961 113 51
PmlI CAC'GTG
Cuts at: 0 765 1125
Size: 765 360
PpuMI rG'GwC_Cy
Cuts at: 0 915 1125
Size: 915 210
PspOMI G'GGCC_C
Cuts at: 0 1025 1125
Size: 1025 100
PstI C_TGCA'G
Cuts at: 0 48 176 266 278 1125
Size: 48 128 90 12 847
Fragments arranged by size:
847 128 90 48 12
PvuII CAG'CTG
Cuts at: 0 800 1125
Size: 800 325
SacII CC_GC'GG
Cuts at: 0 933 1125
Size: 933 192
SapI GCTCTTCn'nnn_
Cuts at: 0 682 1125
Size: 682 443
SfcI C'TryA_G
Cuts at: 0 44 172 262 274 1125
Size: 44 128 90 12 851
Fragments arranged by size:
851 128 90 44 12
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 943 982 1125
Size: 943 39 143
Fragments arranged by size:
943 143 39
StuI AGG'CCT
Cuts at: 0 985 1009 1125
Size: 985 24 116
Fragments arranged by size:
985 116 24
StyI C'CwwG_G
Cuts at: 0 470 812 1125
Size: 470 342 313
Fragments arranged by size:
470 342 313
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 697 922 985 1125
Size: 697 225 63 140
Fragments arranged by size:
697 225 140 63
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1116 1125
Size: 1116 9
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApaI AvaI BanI
BanII BglI BlpI Bme1580I BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BssHII BssSI BstAPI BstYI BtgI BtsI
DraIII EaeI EagI EarI Eco57MI EcoNI EcoO109I FspI
Hin4I HincII MslI MspA1I PasI PflMI PfoI PmlI
PpuMI PspOMI PstI PvuII SacII SapI SfcI SfiI
StuI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaBI BsgI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BsrGI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DrdI EciI EcoICRI EcoRI EcoRV FalI
FseI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI