(Circular) (Six-base) MAPSORT of: pFN21AB7150.seq Check: 2366 from: 1 to: 5746 pFN21AB7150 5746 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3556 278 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 3089 1937 Size: 987 165 4594 Fragments arranged by size: 4594 987 165 Acc65I G'GTAC_C Cuts at: 3067 3067 Size: 5746 AclI AA'CG_TT Cuts at: 1563 3873 4246 1563 Size: 2310 373 3063 Fragments arranged by size: 3063 2310 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2528 3809 5193 5461 1632 Size: 896 1281 1384 268 1917 Fragments arranged by size: 1917 1384 1281 896 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5549 AflIII A'CryG_T Cuts at: 1203 4645 5622 1203 Size: 3442 977 1327 Fragments arranged by size: 3442 1327 977 AgeI A'CCGG_T Cuts at: 4577 4577 Size: 5746 AhdI GACnn_n'nnGTC Cuts at: 4475 4475 Size: 5746 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5746 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5746 AlwNI CAG_nnn'CTG Cuts at: 3489 5061 3489 Size: 1572 4174 ApaI G_GGCC'C Cuts at: 2834 2834 Size: 5746 ApaLI G'TGCA_C Cuts at: 3003 3803 4959 3003 Size: 800 1156 3790 Fragments arranged by size: 3790 1156 800 ApoI r'AATT_y Cuts at: 1493 2909 3055 3281 3433 5542 1493 Size: 1416 146 226 152 2109 1697 Fragments arranged by size: 2109 1697 1416 226 152 146 AseI AT'TA_AT Cuts at: 160 1032 4300 160 Size: 872 3268 1606 Fragments arranged by size: 3268 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2058 3071 1870 Size: 73 67 48 1013 4545 Fragments arranged by size: 4545 1013 73 67 48 BamHI G'GATC_C Cuts at: 3076 3443 3076 Size: 367 5379 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3067 4522 618 Size: 325 217 627 1280 1455 1842 Fragments arranged by size: 1842 1455 1280 627 325 217 BanII G_rGCy'C Cuts at: 1391 2397 2736 2834 2842 3065 1391 Size: 1006 339 98 8 223 4072 Fragments arranged by size: 4072 1006 339 223 98 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4775 BbvCI CC'TCA_GC Cuts at: 2661 2661 Size: 5746 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2638 2672 3937 3971 1903 Size: 34 50 34 617 34 1265 34 3678 Fragments arranged by size: 3678 1265 617 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3638 4848 3638 Size: 1210 4536 BclI T'GATC_A Cuts at: 1548 1548 Size: 5746 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4357 136 Size: 107 122 71 631 605 2685 1525 Fragments arranged by size: 2685 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2299 2557 5741 2299 Size: 258 3184 2304 Fragments arranged by size: 3184 2304 258 BlpI GC'TnA_GC Cuts at: 3163 3163 Size: 5746 Bme1580I G_kGCm'C Cuts at: 1790 2601 2796 2834 3007 3807 4963 1790 Size: 811 195 38 173 800 1156 2573 Fragments arranged by size: 2573 1156 811 800 195 173 38 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5746 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2898 3477 4435 Size: 416 53 83 399 24 1481 579 958 Cuts at: 4435 442 Size: 1753 Fragments arranged by size: 1753 1481 958 579 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5746 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2798 4406 1239 Size: 1265 294 1608 2579 Fragments arranged by size: 2579 1608 1265 294 Bpu10I CC'TnA_GC Cuts at: 2462 2661 2462 Size: 199 5547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2891 3871 4736 5034 2891 Size: 980 865 298 3603 Fragments arranged by size: 3603 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4409 882 Size: 617 2910 2219 Fragments arranged by size: 2910 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3537 493 Size: 711 2333 2702 Fragments arranged by size: 2702 2333 711 BsaBI GATnn'nnATC Cuts at: 5740 5740 Size: 5746 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3553 3935 4570 275 Size: 53 83 186 936 2020 382 635 1451 Fragments arranged by size: 2020 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4179 4577 4851 4998 5409 4179 Size: 398 274 147 411 4516 Fragments arranged by size: 4516 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5521 Fragments arranged by size: 5521 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2076 2120 2678 1186 Size: 890 44 558 4254 Fragments arranged by size: 4254 890 558 44 BseYI C'CCAG_C Cuts at: 4949 5445 4949 Size: 496 5250 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5173 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3196 3957 4985 1540 Size: 275 186 1195 761 1028 2301 Fragments arranged by size: 2301 1195 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2481 2718 3007 3807 3892 4963 2481 Size: 237 289 800 85 1071 3264 Fragments arranged by size: 3264 1071 800 289 237 85 BsmI GAATG_Cn' Cuts at: 3257 3350 3257 Size: 93 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2397 2481 2601 2718 2736 2796 2834 Size: 399 607 84 120 117 18 60 38 Cuts at: 2834 2842 3007 3065 3807 3892 4963 1391 Size: 8 165 58 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 607 399 165 120 117 85 84 60 58 38 18 8 BspEI T'CCGG_A Cuts at: 5409 5409 Size: 5746 BspHI T'CATG_A Cuts at: 3635 5365 3635 Size: 1730 4016 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3034 3101 844 Size: 2190 67 3489 Fragments arranged by size: 3489 2190 67 BsrBI CCG'CTC Cuts at: 1394 1823 3633 1394 Size: 429 1810 3507 Fragments arranged by size: 3507 1810 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4241 4415 65 Size: 1183 2993 174 1396 Fragments arranged by size: 2993 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2999 4390 4577 1700 Size: 110 1189 1391 187 2869 Fragments arranged by size: 2869 1391 1189 187 110 BsrGI T'GTAC_A Cuts at: 96 2373 96 Size: 2277 3469 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5746 BssSI C'ACGA_G Cuts at: 3806 4818 3806 Size: 1012 4734 BstAPI GCAn_nnn'nTGC Cuts at: 794 2368 794 Size: 1574 4172 BstBI TT'CG_AA Cuts at: 4550 4550 Size: 5746 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5746 BstXI CCAn_nnnn'nTGG Cuts at: 2758 2758 Size: 5746 BstYI r'GATC_y Cuts at: 1972 2299 2557 3076 3443 3828 3845 5297 5383 Size: 327 258 519 367 385 17 1452 86 Cuts at: 5383 5395 5741 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 519 385 367 346 327 258 86 17 12 BstZ17I GTA'TAC Cuts at: 2925 2925 Size: 5746 BtgI C'CryG_G Cuts at: 513 1065 2188 513 Size: 552 1123 4071 Fragments arranged by size: 4071 1123 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5007 Fragments arranged by size: 5007 683 56 BtsI GCAGTG_nn' Cuts at: 3262 4056 4076 3262 Size: 794 20 4932 Fragments arranged by size: 4932 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5746 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5711 DraI TTT'AAA Cuts at: 3050 3050 Size: 5746 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4753 809 Size: 507 3437 1802 Fragments arranged by size: 3437 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2880 3193 4082 Size: 54 1425 50 275 18 1050 313 889 Cuts at: 4082 5711 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1050 889 313 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3193 1537 Size: 275 1381 4090 Fragments arranged by size: 4090 1381 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2097 2943 3676 1353 Size: 662 82 846 733 3423 Fragments arranged by size: 3423 846 733 662 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4333 4707 4853 387 Size: 1064 138 2744 374 146 1280 Fragments arranged by size: 2744 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 2528 2798 3809 4406 5193 5461 Size: 393 872 24 270 1011 597 787 268 Cuts at: 5461 1239 Size: 1524 Fragments arranged by size: 1524 1011 872 787 597 393 270 268 24 EcoO109I rG'GnC_Cy Cuts at: 2330 2830 2330 Size: 500 5246 EcoRI G'AATT_C Cuts at: 2909 3055 3433 2909 Size: 146 378 5222 Fragments arranged by size: 5222 378 146 FspI TGC'GCA Cuts at: 4252 4252 Size: 5746 HaeII r_GCGC'y Cuts at: 1678 4893 1678 Size: 3215 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2071 2099 2103 2131 2159 Size: 32 135 32 499 28 4 28 28 Cuts at: 2159 2191 2482 2514 4387 4419 4461 4493 1373 Size: 32 291 32 1873 32 42 32 2626 Fragments arranged by size: 2626 1873 499 291 135 42 32 32 32 32 32 32 28 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2263 2548 3090 3336 669 Size: 988 281 325 285 542 246 3079 Fragments arranged by size: 3079 988 542 325 285 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5746 HpaI GTT'AAC Cuts at: 3336 3336 Size: 5746 KpnI G_GTAC'C Cuts at: 3071 3071 Size: 5746 MfeI C'AATT_G Cuts at: 3345 3345 Size: 5746 MluI A'CGCG_T Cuts at: 5622 5622 Size: 5746 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2202 4860 5044 1305 Size: 897 2658 184 2007 Fragments arranged by size: 2658 2007 897 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2882 10 Size: 54 1425 343 1050 2874 Fragments arranged by size: 2874 1425 1050 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2535 3704 4063 4222 518 Size: 648 1369 1169 359 159 2042 Fragments arranged by size: 2042 1369 1169 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2231 3162 3839 4987 5232 1606 Size: 625 931 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 625 245 NcoI C'CATG_G Cuts at: 513 1065 2188 513 Size: 552 1123 4071 Fragments arranged by size: 4071 1123 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5746 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5746 NotI GC'GGCC_GC Cuts at: 3193 3193 Size: 5746 NspI r_CATG'y Cuts at: 1132 1229 3104 4649 1132 Size: 97 1875 1545 2229 Fragments arranged by size: 2229 1875 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5746 PciI A'CATG_T Cuts at: 4645 4645 Size: 5746 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5746 PmeI GTTT'AAAC Cuts at: 3050 3050 Size: 5746 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3815 3847 5328 5360 3815 Size: 32 1481 32 4201 Fragments arranged by size: 4201 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5746 PsiI TTA'TAA Cuts at: 3316 3316 Size: 5746 PspOMI G'GGCC_C Cuts at: 2830 2830 Size: 5746 PspXI vC'TCGA_Gb Cuts at: 2010 2010 Size: 5746 PstI C_TGCA'G Cuts at: 830 3045 3098 830 Size: 2215 53 3478 Fragments arranged by size: 3478 2215 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5746 PvuII CAG'CTG Cuts at: 2231 2231 Size: 5746 SalI G'TCGA_C Cuts at: 1936 3088 1936 Size: 1152 4594 SbfI CC_TGCA'GG Cuts at: 3098 3098 Size: 5746 ScaI AGT'ACT Cuts at: 3994 5706 3994 Size: 1712 4034 SfcI C'TryA_G Cuts at: 826 1045 2226 2334 3041 3094 4229 4910 5101 Size: 219 1181 108 707 53 1135 681 191 Cuts at: 5101 826 Size: 1471 Fragments arranged by size: 1471 1181 1135 707 681 219 191 108 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5746 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5746 SmaI CCC'GGG Cuts at: 1872 3073 1872 Size: 1201 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2010 2870 3850 4751 5013 820 Size: 197 926 67 860 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 860 262 197 67 SnaBI TAC'GTA Cuts at: 493 3537 493 Size: 3044 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5746 SphI G_CATG'C Cuts at: 1132 3104 1132 Size: 1972 3774 SspI AAT'ATT Cuts at: 5 52 3670 5 Size: 47 3618 2081 Fragments arranged by size: 3618 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5746 StyI C'CwwG_G Cuts at: 513 1065 2188 2883 2976 3012 3185 513 Size: 552 1123 695 93 36 173 3074 Fragments arranged by size: 3074 1123 695 552 173 93 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3773 3790 3943 4128 4573 325 Size: 1255 2193 17 153 185 445 1498 Fragments arranged by size: 2193 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2373 3992 5704 Size: 274 80 33 51 1443 396 1619 1712 Cuts at: 5704 96 Size: 138 Fragments arranged by size: 1712 1619 1443 396 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2233 2265 5706 5738 2233 Size: 32 3441 32 2241 Fragments arranged by size: 3441 2241 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5746 XbaI T'CTAG_A Cuts at: 2302 3082 2302 Size: 780 4966 XhoI C'TCGA_G Cuts at: 1943 2010 1943 Size: 67 5679 XmaI C'CCGG_G Cuts at: 1870 3071 1870 Size: 1201 4545 XmnI GAAnn'nnTTC Cuts at: 3875 3875 Size: 5746 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3554 276 Size: 53 83 186 936 2020 2468 Fragments arranged by size: 2468 2020 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI