Restriction map for
pFN21AB7149
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BclI BcnI BfrI BlnI
BlpI BpmI Bpu1102I BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MamI
MfeI MluI MroI MspI MunI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XcmI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 664 AAATTC [GA]^AATT[CT]
AcyI 971 GGCGTC G[GA]^CG[CT]C
AhdI 63 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 181 CCTCAGG CC^T[ACGT]AGG
ApoI 664 AAATTC [GA]^AATT[CT]
AspEI 63 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 499 GAAGAC GAAGAC(2/6)
BciVI 138 GGATAC GTATCC(6/5)
BglI 325 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 499 GAAGAC GAAGAC(2/6)
BsaAI 359 TACGTA [CT]AC^GT[GA]
BsaHI 971 GGCGTC G[GA]^CG[CT]C
BsgI 748 GTGCAG GTGCAG(16/14)
BsmFI 578 GGGAC GGGAC(10/14)
BspHI 668 TCATGA T^CATGA
BsrDI 569 GCAATG GCAATG(2/0)
BsrI 956 CCAGT ACTGG(1/-1)
BssSI 32 CTCGTG CACGAG(-5/-1)
Bsu36I 181 CCTCAGG CC^T[ACGT]AGG
Cac8I 163 GCTGGC GC[ACGT]^[ACGT]GC
DraII 1024 AGGTCCT [GA]G^G[ACGT]CC[CT]
Eam1105I 63 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 181 CCTCAGG CC^T[ACGT]AGG
EcoNI 127 CCTGTTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1024 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 255 CCAAGG C^C[AT][AT]GG
HgaI 972 GCGTC GACGC(5/10)
Hin1I 971 GGCGTC G[GA]^CG[CT]C
HinfI 232 GAATC G^A[ACGT]TC
MaeII 360 ACGT A^CGT
MaeIII 893 GTGAC ^GT[ACGT]AC
MspA1I 215 CAGCTG C[AC]G^C[GT]G
PaeR7I 856 CTCGAG C^TCGAG
PflMI 738 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1024 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 215 CAGCTG CAG^CTG
RcaI 668 TCATGA T^CATGA
SnaBI 359 TACGTA TAC^GTA
StyI 255 CCAAGG C^C[AT][AT]GG
TaqI 857 TCGA T^CGA
TfiI 232 GAATC G^A[AT]TC
Tsp45I 893 GTGAC ^GT[GC]AC
TthHB8I 857 TCGA T^CGA
Van91I 738 CCAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1006 TCTAGA T^CTAGA
XhoI 856 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 53 CGCG CG^CG
1001 CGCG
AciI 45 CCGC CCGC(-3/-1)
465 CCGC
AvaI 5 CTCGGG C^[CT]CG[GA]G
856 CTCGAG
AvaII 817 GGTCC G^G[AT]CC
1025 GGTCC
BalI 513 TGGCCA TGG^CCA
866 TGGCCA
BfaI 509 CTAG C^TAG
1007 CTAG
BglII 656 AGATCT A^GATCT
741 AGATCT
BsaI 61 GAGACC GGTCTC(1/5)
70 GGTCTC
BsmAI 61 GAGAC GTCTC(1/5)
71 GTCTC
BsmI 338 GAATGC GAATGC(1/-1)
167 GCATTC
BsoBI 5 CTCGGG C^[CT]CG[GA]G
856 CTCGAG
BspMI 774 GCAGGT ACCTGC(4/8)
1022 GCAGGT
BstF5I 293 CATCC GGATG(2/0)
902 CATCC
BstUI 53 CGCG CG^CG
1001 CGCG
CfoI 52 GCGC GCG^C
1000 GCGC
Cfr13I 817 GGTCC G^G[ACGT]CC
1025 GGTCC
EaeI 513 TGGCCA [CT]^GGCC[GA]
866 TGGCCA
FokI 293 CATCC GGATG(9/13)
902 CATCC
HhaI 52 GCGC GCG^C
1000 GCGC
HinP1I 52 GCGC G^CGC
1000 GCGC
MaeI 509 CTAG C^TAG
1007 CTAG
MluNI 513 TGGCCA TGG^CCA
866 TGGCCA
MscI 513 TGGCCA TGG^CCA
866 TGGCCA
MseI 628 TTAA T^TAA
706 TTAA
MslI 669 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
840 CACCAAGATG
MvnI 53 CGCG CG^CG
1001 CGCG
PstI 92 CTGCAG CTGCA^G
212 CTGCAG
Sau96I 817 GGTCC G^G[ACGT]CC
1025 GGTCC
SfaNI 345 GATGC GCATC(5/9)
603 GATGC
SmlI 73 CTCAAG C^T[CT][GA]AG
856 CTCGAG
Tru9I 628 TTAA T^TAA
706 TTAA
Tsp509I 665 AATT ^AATT
806 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI AlwI AspHI BanI
BanII BbvI BmyI BsaJI BseRI
BsiHKAI BsiYI BslI Bsp1286I BssKI
BstNI BstYI DdeI DpnII EarI
EcoRII Fnu4HI HaeIII HphI ItaI
Ksp632I MboI MboII MflI MnlI
MvaI MwoI NdeII NlaIII NlaIV
RsaI SacI Sau3AI ScrFI SfcI
TseI TspRI XhoII