Restriction map for
pFN21AB7149
(Circular) (Six-base) MAPSORT of: pFN21AB7149.seq Check: 6651 from: 1 to: 5746
pFN21AB7149 5746 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2770 2770
Size: 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3556 278
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3089 1937
Size: 1152 4594
Acc65I G'GTAC_C
Cuts at: 3067 3067
Size: 5746
AclI AA'CG_TT
Cuts at: 1563 3873 4246 1563
Size: 2310 373 3063
Fragments arranged by size:
3063 2310 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2429 2789 3809 5193 5461 1632
Size: 797 360 1020 1384 268 1917
Fragments arranged by size:
1917 1384 1020 797 360 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5549
AflIII A'CryG_T
Cuts at: 1203 4645 5622 1203
Size: 3442 977 1327
Fragments arranged by size:
3442 1327 977
AgeI A'CCGG_T
Cuts at: 4577 4577
Size: 5746
AhdI GACnn_n'nnGTC
Cuts at: 2073 4475 2073
Size: 2402 3344
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5746
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5746
AlwNI CAG_nnn'CTG
Cuts at: 3489 5061 3489
Size: 1572 4174
ApaLI G'TGCA_C
Cuts at: 3803 4959 3803
Size: 1156 4590
ApoI r'AATT_y
Cuts at: 1493 2669 3055 3281 3433 5542 1493
Size: 1176 386 226 152 2109 1697
Fragments arranged by size:
2109 1697 1176 386 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4300 160
Size: 872 3268 1606
Fragments arranged by size:
3268 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
AvaI C'yCGr_G
Cuts at: 1870 1943 2010 2861 3071 1870
Size: 73 67 851 210 4545
Fragments arranged by size:
4545 851 210 73 67
BamHI G'GATC_C
Cuts at: 3076 3443 3076
Size: 367 5379
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2249 2782 2885 3067 4522
Size: 325 217 627 462 533 103 182 1455
Cuts at: 4522 618
Size: 1842
Fragments arranged by size:
1842 1455 627 533 462 325 217 182
103
BanII G_rGCy'C
Cuts at: 1391 2197 2312 2388 2481 2950 3019 3065 1391
Size: 806 115 76 93 469 69 46 4072
Fragments arranged by size:
4072 806 469 115 93 76 69 46
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2511 928
Size: 971 612 4163
Fragments arranged by size:
4163 971 612
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3937 3971 1903
Size: 34 50 34 1916 34 3678
Fragments arranged by size:
3678 1916 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2137 3638 4848 2137
Size: 1501 1210 3035
Fragments arranged by size:
3035 1501 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5746
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2336 4357 136
Size: 107 122 71 631 605 664 2021 1525
Fragments arranged by size:
2021 1525 664 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2661 2746 5741 2661
Size: 85 2995 2666
Fragments arranged by size:
2995 2666 85
BlpI GC'TnA_GC
Cuts at: 3163 3163
Size: 5746
Bme1580I G_kGCm'C
Cuts at: 1790 2120 2787 3807 4963 1790
Size: 330 667 1020 1156 2573
Fragments arranged by size:
2573 1156 1020 667 330
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5746
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3477 4435 442
Size: 416 53 83 399 24 2060 958 1753
Fragments arranged by size:
2060 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5746
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2137 2137
Size: 5746
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4406 1239
Size: 3167 2579
Bpu10I CC'TnA_GC
Cuts at: 2570 2570
Size: 5746
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2063 3871 4736 5034 2063
Size: 1808 865 298 2775
Fragments arranged by size:
2775 1808 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2081 4409 882
Size: 617 561 21 2328 2219
Fragments arranged by size:
2328 2219 617 561 21
BsaAI yAC'GTr
Cuts at: 493 1204 2366 3537 493
Size: 711 1162 1171 2702
Fragments arranged by size:
2702 1171 1162 711
BsaBI GATnn'nnATC
Cuts at: 5740 5740
Size: 5746
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2977 3553 3935 4570
Size: 53 83 186 936 1444 576 382 635
Cuts at: 4570 275
Size: 1451
Fragments arranged by size:
1451 1444 936 635 576 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4179 4577 4851 4998 5409 4179
Size: 398 274 147 411 4516
Fragments arranged by size:
4516 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5521
Fragments arranged by size:
5521 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 2113 2116 2126 2717 1186
Size: 892 35 3 10 591 4215
Fragments arranged by size:
4215 892 591 35 10 3
BseYI C'CCAG_C
Cuts at: 4949 5445 4949
Size: 496 5250
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2774 1163
Size: 573 1038 4135
Fragments arranged by size:
4135 1038 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3196 3957 4985 1540
Size: 275 186 1195 761 1028 2301
Fragments arranged by size:
2301 1195 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2038 2197 2312 2481 2709 3807 3892 4963 2038
Size: 159 115 169 228 1098 85 1071 2821
Fragments arranged by size:
2821 1098 1071 228 169 159 115 85
BsmI GAATG_Cn'
Cuts at: 2172 2349 3257 3350 2172
Size: 177 908 93 4568
Fragments arranged by size:
4568 908 177 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2120 2197 2312 2388 2481 2709
Size: 399 248 82 77 115 76 93 228
Cuts at: 2709 2787 2950 3019 3065 3807 3892 4963 1391
Size: 78 163 69 46 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 248 228 163 115
93 85 82 78 77 76 69 46
BspEI T'CCGG_A
Cuts at: 5409 5409
Size: 5746
BspHI T'CATG_A
Cuts at: 2673 3635 5365 2673
Size: 962 1730 3054
Fragments arranged by size:
3054 1730 962
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2770 3018 3101 844
Size: 1926 248 83 3489
Fragments arranged by size:
3489 1926 248 83
BsrBI CCG'CTC
Cuts at: 1394 1823 3633 1394
Size: 429 1810 3507
Fragments arranged by size:
3507 1810 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2581 4241 4415 65
Size: 1183 1333 1660 174 1396
Fragments arranged by size:
1660 1396 1333 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4390 4577 1700
Size: 110 2580 187 2869
Fragments arranged by size:
2869 2580 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5746
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5746
BssSI C'ACGA_G
Cuts at: 2037 3806 4818 2037
Size: 1769 1012 2965
Fragments arranged by size:
2965 1769 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5746
BstBI TT'CG_AA
Cuts at: 4550 4550
Size: 5746
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5746
BstYI r'GATC_y
Cuts at: 1972 2320 2448 2661 2746 3076 3443 3828 3845
Size: 348 128 213 85 330 367 385 17
Cuts at: 3845 5297 5383 5395 5741 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 348 346 330 213
128 86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2187 2187
Size: 5746
BtgI C'CryG_G
Cuts at: 513 1065 3042 513
Size: 552 1977 3217
Fragments arranged by size:
3217 1977 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5007
Fragments arranged by size:
5007 683 56
BtsI GCAGTG_nn'
Cuts at: 2094 2208 3262 4056 4076 2094
Size: 114 1054 794 20 3764
Fragments arranged by size:
3764 1054 794 114 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5746
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2497 2532 529
Size: 35 1933 35 3743
Fragments arranged by size:
3743 1933 35 35
DraI TTT'AAA
Cuts at: 3050 3050
Size: 5746
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4753 809
Size: 507 3437 1802
Fragments arranged by size:
3437 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2518 2871 3193
Size: 54 1425 50 275 18 688 353 322
Cuts at: 3193 4082 5711 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 688 353 322 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3193 1537
Size: 275 1381 4090
Fragments arranged by size:
4090 1381 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2652 2913 2934 3676 1353
Size: 662 637 261 21 742 3423
Fragments arranged by size:
3423 742 662 637 261 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4333 4707 4853 387
Size: 1064 138 2744 374 146 1280
Fragments arranged by size:
2744 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2195 2310 2479 2195
Size: 115 169 5462
Fragments arranged by size:
5462 169 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2429 2789 3809 4406 5193 5461 1239
Size: 393 797 360 1020 597 787 268 1524
Fragments arranged by size:
1524 1020 797 787 597 393 360 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2136 2136
Size: 5746
EcoO109I rG'GnC_Cy
Cuts at: 3030 3030
Size: 5746
EcoRI G'AATT_C
Cuts at: 3055 3433 3055
Size: 378 5368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2455 2455
Size: 5746
FspI TGC'GCA
Cuts at: 4252 4252
Size: 5746
HaeII r_GCGC'y
Cuts at: 1678 4893 1678
Size: 3215 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2891 2923 2937
Size: 32 135 32 487 32 800 32 14
Cuts at: 2937 2969 4387 4419 4461 4493 1373
Size: 32 1418 32 42 32 2626
Fragments arranged by size:
2626 1418 800 487 135 42 32 32
32 32 32 32 32 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3090 3336 669
Size: 988 281 1152 246 3079
Fragments arranged by size:
3079 1152 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5746
HpaI GTT'AAC
Cuts at: 3336 3336
Size: 5746
KpnI G_GTAC'C
Cuts at: 3071 3071
Size: 5746
MfeI C'AATT_G
Cuts at: 3345 3345
Size: 5746
MluI A'CGCG_T
Cuts at: 5622 5622
Size: 5746
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4860 5044 1305
Size: 3555 184 2007
Fragments arranged by size:
3555 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2520 2873 10
Size: 54 1425 343 688 353 2883
Fragments arranged by size:
2883 1425 688 353 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2678 2849 3704 4063 4222 518
Size: 648 1512 171 855 359 159 2042
Fragments arranged by size:
2042 1512 855 648 359 171 159
MspA1I CmG'CkG
Cuts at: 1606 2222 3162 3839 4987 5232 1606
Size: 616 940 677 1148 245 2120
Fragments arranged by size:
2120 1148 940 677 616 245
NcoI C'CATG_G
Cuts at: 513 1065 3042 513
Size: 552 1977 3217
Fragments arranged by size:
3217 1977 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5746
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5746
NotI GC'GGCC_GC
Cuts at: 3193 3193
Size: 5746
NspI r_CATG'y
Cuts at: 1132 1229 3104 4649 1132
Size: 97 1875 1545 2229
Fragments arranged by size:
2229 1875 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2071 1348
Size: 723 5023
PciI A'CATG_T
Cuts at: 4645 4645
Size: 5746
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2749 3043 1108
Size: 1641 294 3811
Fragments arranged by size:
3811 1641 294
PmeI GTTT'AAAC
Cuts at: 3050 3050
Size: 5746
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3815 3847 5328 5360 3815
Size: 32 1481 32 4201
Fragments arranged by size:
4201 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3030 3030
Size: 5746
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5746
PsiI TTA'TAA
Cuts at: 3316 3316
Size: 5746
PspXI vC'TCGA_Gb
Cuts at: 2861 2861
Size: 5746
PstI C_TGCA'G
Cuts at: 830 2101 2221 3098 830
Size: 1271 120 877 3478
Fragments arranged by size:
3478 1271 877 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5746
PvuII CAG'CTG
Cuts at: 2222 2222
Size: 5746
SacI G_AGCT'C
Cuts at: 2197 2312 2481 2197
Size: 115 169 5462
Fragments arranged by size:
5462 169 115
SalI G'TCGA_C
Cuts at: 1936 3088 1936
Size: 1152 4594
SbfI CC_TGCA'GG
Cuts at: 3098 3098
Size: 5746
ScaI AGT'ACT
Cuts at: 3994 5706 3994
Size: 1712 4034
SfcI C'TryA_G
Cuts at: 826 1045 2097 2217 2325 3094 4229 4910 5101
Size: 219 1052 120 108 769 1135 681 191
Cuts at: 5101 826
Size: 1471
Fragments arranged by size:
1471 1135 1052 769 681 219 191 120
108
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5746
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5746
SmaI CCC'GGG
Cuts at: 1872 3073 1872
Size: 1201 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2078 2861 3850 4751 5013 820
Size: 197 926 135 783 989 901 262 1553
Fragments arranged by size:
1553 989 926 901 783 262 197 135
SnaBI TAC'GTA
Cuts at: 493 2366 3537 493
Size: 1873 1171 2702
Fragments arranged by size:
2702 1873 1171
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5746
SphI G_CATG'C
Cuts at: 1132 3104 1132
Size: 1972 3774
SspI AAT'ATT
Cuts at: 5 52 3670 5
Size: 47 3618 2081
Fragments arranged by size:
3618 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5746
StyI C'CwwG_G
Cuts at: 513 1065 2260 3042 3185 513
Size: 552 1195 782 143 3074
Fragments arranged by size:
3074 1195 782 552 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2215 2609 2756 3773 3790 3943 4128
Size: 1255 635 394 147 1017 17 153 185
Cuts at: 4128 4573 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1017 635 445 394 185 153
147 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2427 3992 5704
Size: 274 80 33 51 1443 450 1565 1712
Cuts at: 5704 96
Size: 138
Fragments arranged by size:
1712 1565 1443 450 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5706 5738 5706
Size: 32 5714
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5746
XbaI T'CTAG_A
Cuts at: 3011 3082 3011
Size: 71 5675
XhoI C'TCGA_G
Cuts at: 1943 2861 1943
Size: 918 4828
XmaI C'CCGG_G
Cuts at: 1870 3071 1870
Size: 1201 4545
XmnI GAAnn'nnTTC
Cuts at: 3875 3875
Size: 5746
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3554 276
Size: 53 83 186 936 2020 2468
Fragments arranged by size:
2468 2020 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PspOMI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI XcmI