Restriction map for
pFN21AB7149
(Linear) (Six-base) MAPSORT of: KIBB7149.seq Check: 4545 from: 1 to: 1041
KIBB7149.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 765 1041
Size: 765 276
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 784 1041
Size: 424 360 257
Fragments arranged by size:
424 360 257
AhdI GACnn_n'nnGTC
Cuts at: 0 68 1041
Size: 68 973
ApoI r'AATT_y
Cuts at: 0 664 1041
Size: 664 377
AvaI C'yCGr_G
Cuts at: 0 5 856 1041
Size: 5 851 185
Fragments arranged by size:
851 185 5
BanI G'GyrC_C
Cuts at: 0 244 777 880 1041
Size: 244 533 103 161
Fragments arranged by size:
533 244 161 103
BanII G_rGCy'C
Cuts at: 0 192 307 383 476 945 1014 1041
Size: 192 115 76 93 469 69 27
Fragments arranged by size:
469 192 115 93 76 69 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 506 1041
Size: 506 535
BciVI GTATCCnnnnn_n'
Cuts at: 0 132 1041
Size: 132 909
BglI GCCn_nnn'nGGC
Cuts at: 0 331 1041
Size: 331 710
BglII A'GATC_T
Cuts at: 0 656 741 1041
Size: 656 85 300
Fragments arranged by size:
656 300 85
Bme1580I G_kGCm'C
Cuts at: 0 115 782 1041
Size: 115 667 259
Fragments arranged by size:
667 259 115
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 132 1041
Size: 132 909
Bpu10I CC'TnA_GC
Cuts at: 0 565 1041
Size: 565 476
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 1041
Size: 58 983
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 76 1041
Size: 55 21 965
Fragments arranged by size:
965 55 21
BsaAI yAC'GTr
Cuts at: 0 361 1041
Size: 361 680
BsaHI Gr'CG_yC
Cuts at: 0 972 1041
Size: 972 69
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 108 111 121 712 1041
Size: 73 35 3 10 591 329
Fragments arranged by size:
591 329 73 35 10 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1041
Size: 769 272
BsiHKAI G_wGCw'C
Cuts at: 0 33 192 307 476 704 1041
Size: 33 159 115 169 228 337
Fragments arranged by size:
337 228 169 159 115 33
BsmI GAATG_Cn'
Cuts at: 0 167 344 1041
Size: 167 177 697
Fragments arranged by size:
697 177 167
Bsp1286I G_dGCh'C
Cuts at: 0 33 115 192 307 383 476 704 782
Size: 33 82 77 115 76 93 228 78
Cuts at: 782 945 1014 1041
Size: 163 69 27
Fragments arranged by size:
228 163 115 93 82 78 77 76
69 33 27
BspHI T'CATG_A
Cuts at: 0 668 1041
Size: 668 373
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 765 1013 1041
Size: 765 248 28
Fragments arranged by size:
765 248 28
BsrDI GCAATG_nn'
Cuts at: 0 576 1041
Size: 576 465
BssSI C'ACGA_G
Cuts at: 0 32 1041
Size: 32 1009
BstYI r'GATC_y
Cuts at: 0 315 443 656 741 1041
Size: 315 128 213 85 300
Fragments arranged by size:
315 300 213 128 85
Bsu36I CC'TnA_GG
Cuts at: 0 182 1041
Size: 182 859
BtsI GCAGTG_nn'
Cuts at: 0 89 203 1041
Size: 89 114 838
Fragments arranged by size:
838 114 89
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 492 527 1041
Size: 492 35 514
Fragments arranged by size:
514 492 35
EaeI y'GGCC_r
Cuts at: 0 513 866 1041
Size: 513 353 175
Fragments arranged by size:
513 353 175
EarI CTCTTCn'nnn_
Cuts at: 0 10 647 908 929 1041
Size: 10 637 261 21 112
Fragments arranged by size:
637 261 112 21 10
EcoICRI GAG'CTC
Cuts at: 0 190 305 474 1041
Size: 190 115 169 567
Fragments arranged by size:
567 190 169 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 784 1041
Size: 424 360 257
Fragments arranged by size:
424 360 257
EcoNI CCTnn'n_nnAGG
Cuts at: 0 131 1041
Size: 131 910
EcoO109I rG'GnC_Cy
Cuts at: 0 1025 1041
Size: 1025 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 450 1041
Size: 450 591
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 886 918 932 964 1041
Size: 54 32 800 32 14 32 77
Fragments arranged by size:
800 77 54 32 32 32 14
MscI TGG'CCA
Cuts at: 0 515 868 1041
Size: 515 353 173
Fragments arranged by size:
515 353 173
MslI CAynn'nnrTG
Cuts at: 0 673 844 1041
Size: 673 171 197
Fragments arranged by size:
673 197 171
MspA1I CmG'CkG
Cuts at: 0 217 1041
Size: 217 824
PasI CC'CwG_GG
Cuts at: 0 66 1041
Size: 66 975
PflMI CCAn_nnn'nTGG
Cuts at: 0 744 1041
Size: 744 297
PpuMI rG'GwC_Cy
Cuts at: 0 1025 1041
Size: 1025 16
PspXI vC'TCGA_Gb
Cuts at: 0 856 1041
Size: 856 185
PstI C_TGCA'G
Cuts at: 0 96 216 1041
Size: 96 120 825
Fragments arranged by size:
825 120 96
PvuII CAG'CTG
Cuts at: 0 217 1041
Size: 217 824
SacI G_AGCT'C
Cuts at: 0 192 307 476 1041
Size: 192 115 169 565
Fragments arranged by size:
565 192 169 115
SfcI C'TryA_G
Cuts at: 0 92 212 320 1041
Size: 92 120 108 721
Fragments arranged by size:
721 120 108 92
SmlI C'TyrA_G
Cuts at: 0 73 856 1041
Size: 73 783 185
Fragments arranged by size:
783 185 73
SnaBI TAC'GTA
Cuts at: 0 361 1041
Size: 361 680
StyI C'CwwG_G
Cuts at: 0 255 1041
Size: 255 786
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 210 604 751 1041
Size: 210 394 147 290
Fragments arranged by size:
394 290 210 147
TatI w'GTAC_w
Cuts at: 0 422 1041
Size: 422 619
XbaI T'CTAG_A
Cuts at: 0 1006 1041
Size: 1006 35
XhoI C'TCGA_G
Cuts at: 0 856 1041
Size: 856 185
Enzymes that do cut:
AarI AcuI AhdI ApoI AvaI BanI BanII BbsI
BciVI BglI BglII Bme1580I BplI Bpu10I BpuEI BsaI
BsaAI BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrDI BssSI BstYI Bsu36I BtsI CspCI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MscI
MslI MspA1I PasI PflMI PpuMI PspXI PstI PvuII
SacI SfcI SmlI SnaBI StyI TaqII TatI XbaI
XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BfrBI BlpI BmgBI BmrI BmtI BpmI BsaBI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgI
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XcmI
XmaI XmnI ZraI