(Circular) (Six-base) MAPSORT of: pFN21AB7147.seq Check: 9787 from: 1 to: 5743 pFN21AB7147 5743 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 2817 2829 3086 1937 Size: 517 363 12 257 4594 Fragments arranged by size: 4594 517 363 257 12 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2951 3806 5190 5458 1632 Size: 1319 855 1384 268 1917 Fragments arranged by size: 1917 1384 1319 855 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 2730 4642 5619 1203 Size: 1527 1912 977 1327 Fragments arranged by size: 1912 1527 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 4472 4472 Size: 5743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5743 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2610 3486 5058 2610 Size: 876 1572 3295 Fragments arranged by size: 3295 1572 876 ApaI G_GGCC'C Cuts at: 2724 2724 Size: 5743 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2465 3052 3278 3430 5539 1493 Size: 972 587 226 152 2109 1697 Fragments arranged by size: 2109 1697 972 587 226 152 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 3068 1870 Size: 73 1125 4545 Fragments arranged by size: 4545 1125 73 BamHI G'GATC_C Cuts at: 2631 3073 3440 2631 Size: 442 367 4934 Fragments arranged by size: 4934 442 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 3064 4519 618 Size: 325 217 627 708 569 1455 1842 Fragments arranged by size: 1842 1455 708 627 569 325 217 BanII G_rGCy'C Cuts at: 1391 2091 2724 2895 3062 1391 Size: 700 633 171 167 4072 Fragments arranged by size: 4072 700 633 171 167 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2047 2110 928 Size: 971 148 63 4561 Fragments arranged by size: 4561 971 148 63 BbvCI CC'TCA_GC Cuts at: 2876 2876 Size: 5743 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3934 3968 1903 Size: 34 1997 34 3678 Fragments arranged by size: 3678 1997 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2002 3635 4845 2002 Size: 1633 1210 2900 Fragments arranged by size: 2900 1633 1210 BclI T'GATC_A Cuts at: 1548 2595 1548 Size: 1047 4696 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2557 4354 136 Size: 107 122 71 631 605 885 1797 1525 Fragments arranged by size: 1797 1525 885 631 605 122 107 71 BglII A'GATC_T Cuts at: 5738 5738 Size: 5743 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2498 2724 3804 4960 1790 Size: 708 226 1080 1156 2573 Fragments arranged by size: 2573 1156 1080 708 226 BmgBI CAC'GTC Cuts at: 1318 2158 1318 Size: 840 4903 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3474 4432 442 Size: 416 53 83 399 24 2057 958 1753 Fragments arranged by size: 2057 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2540 1056 Size: 1484 4259 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2537 2582 4403 1239 Size: 1298 45 1821 2579 Fragments arranged by size: 2579 1821 1298 45 Bpu10I CC'TnA_GC Cuts at: 2876 2876 Size: 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3868 4733 5031 3868 Size: 865 298 4580 Fragments arranged by size: 4580 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4406 882 Size: 617 2907 2219 Fragments arranged by size: 2907 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2785 3534 493 Size: 711 1581 749 2702 Fragments arranged by size: 2702 1581 749 711 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2523 4176 4574 4848 4995 5406 2523 Size: 1653 398 274 147 411 2860 Fragments arranged by size: 2860 1653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2822 2825 2852 2855 2872 Size: 30 165 30 1419 3 27 3 17 Cuts at: 2872 2902 1178 Size: 30 4019 Fragments arranged by size: 4019 1419 165 30 30 30 27 17 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2680 2830 2833 2893 1186 Size: 1494 150 3 60 4036 Fragments arranged by size: 4036 1494 150 60 3 BseYI C'CCAG_C Cuts at: 2389 4946 5442 2389 Size: 2557 496 2690 Fragments arranged by size: 2690 2557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5170 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2384 3193 3954 4982 1540 Size: 275 186 383 809 761 1028 2301 Fragments arranged by size: 2301 1028 809 761 383 275 186 BsiHKAI G_wGCw'C Cuts at: 2163 3804 3889 4960 2163 Size: 1641 85 1071 2946 Fragments arranged by size: 2946 1641 1071 85 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2163 2498 2724 2895 3062 3804 Size: 399 301 72 335 226 171 167 742 Cuts at: 3804 3889 4960 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 301 226 171 167 85 72 BspEI T'CCGG_A Cuts at: 2523 5406 2523 Size: 2883 2860 BspHI T'CATG_A Cuts at: 2866 3632 5362 2866 Size: 766 1730 3247 Fragments arranged by size: 3247 1730 766 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 2196 2390 2516 3098 844 Size: 1265 87 194 126 582 3489 Fragments arranged by size: 3489 1265 582 194 126 87 BsrBI CCG'CTC Cuts at: 1394 1823 2963 3630 1394 Size: 429 1140 667 3507 Fragments arranged by size: 3507 1140 667 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2658 4387 4574 1700 Size: 110 848 1729 187 2869 Fragments arranged by size: 2869 1729 848 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5743 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 3803 4815 3803 Size: 1012 4731 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2631 2778 3073 3440 3825 3842 5294 5380 Size: 659 147 295 367 385 17 1452 86 Cuts at: 5380 5392 5738 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 659 385 367 346 295 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 513 Size: 552 939 147 4105 Fragments arranged by size: 4105 939 552 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2440 3259 4053 4073 2440 Size: 819 794 20 4110 Fragments arranged by size: 4110 819 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 3047 3047 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2825 4750 809 Size: 507 1509 1925 1802 Fragments arranged by size: 1925 1802 1509 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2148 2381 2688 Size: 54 1425 50 275 18 318 233 307 Cuts at: 2688 3019 3190 4079 5708 8 Size: 331 171 889 1629 43 Fragments arranged by size: 1629 1425 889 331 318 307 275 233 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2381 3190 1537 Size: 275 569 809 4090 Fragments arranged by size: 4090 809 569 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2321 2946 3673 1353 Size: 968 625 727 3423 Fragments arranged by size: 3423 968 727 625 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2537 2582 2951 3806 4403 5190 5458 Size: 393 905 45 369 855 597 787 268 Cuts at: 5458 1239 Size: 1524 Fragments arranged by size: 1524 905 855 787 597 393 369 268 45 EcoO109I rG'GnC_Cy Cuts at: 2015 2505 2721 2015 Size: 490 216 5037 Fragments arranged by size: 5037 490 216 EcoRI G'AATT_C Cuts at: 2465 3052 3430 2465 Size: 587 378 4778 Fragments arranged by size: 4778 587 378 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 2140 4890 1678 Size: 462 2750 2531 Fragments arranged by size: 2750 2531 462 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4384 4416 4458 4490 1373 Size: 32 135 32 2812 32 42 32 2626 Fragments arranged by size: 2812 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2818 3087 3333 669 Size: 988 281 880 269 246 3079 Fragments arranged by size: 3079 988 880 281 269 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5743 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 2730 5619 2730 Size: 2889 2854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3021 10 Size: 54 1425 343 1189 2732 Fragments arranged by size: 2732 1425 1189 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3701 4060 4219 518 Size: 648 2535 359 159 2042 Fragments arranged by size: 2535 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2327 2593 2975 3159 3836 4984 5229 1606 Size: 721 266 382 184 677 1148 245 2120 Fragments arranged by size: 2120 1148 721 677 382 266 245 184 NaeI GCC'GGC Cuts at: 2660 2660 Size: 5743 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4252 Fragments arranged by size: 4252 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NgoMIV G'CCGG_C Cuts at: 2658 2658 Size: 5743 NheI G'CTAG_C Cuts at: 1052 2536 1052 Size: 1484 4259 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 2242 3101 4646 1132 Size: 97 1013 859 1545 2229 Fragments arranged by size: 2229 1545 1013 859 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2276 2743 1108 Size: 1168 467 4108 Fragments arranged by size: 4108 1168 467 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2505 2505 Size: 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PspOMI G'GGCC_C Cuts at: 2720 2720 Size: 5743 PstI C_TGCA'G Cuts at: 830 2136 2447 3095 830 Size: 1306 311 648 3478 Fragments arranged by size: 3478 1306 648 311 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 PvuII CAG'CTG Cuts at: 2327 2975 2327 Size: 648 5095 RsrII CG'GwC_CG Cuts at: 2965 2965 Size: 5743 SalI G'TCGA_C Cuts at: 1936 2816 3085 1936 Size: 880 269 4594 Fragments arranged by size: 4594 880 269 SapI GCTCTTCn'nnn_ Cuts at: 2946 2946 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2132 2443 2614 3091 4226 4907 5098 Size: 219 1087 311 171 477 1135 681 191 Cuts at: 5098 826 Size: 1471 Fragments arranged by size: 1471 1135 1087 681 477 311 219 191 171 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 3070 1872 Size: 1198 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3847 4748 5010 820 Size: 197 926 1904 901 262 1553 Fragments arranged by size: 1904 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 2242 3101 1132 Size: 1110 859 3774 Fragments arranged by size: 3774 1110 859 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2004 3182 513 Size: 552 939 1178 3074 Fragments arranged by size: 3074 1178 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2376 2982 3770 3787 3940 4125 4570 Size: 1255 796 606 788 17 153 185 445 Cuts at: 4570 325 Size: 1498 Fragments arranged by size: 1498 1255 796 788 606 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3989 5701 96 Size: 274 80 33 51 1443 2012 1712 138 Fragments arranged by size: 2012 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2872 2904 5703 5735 2872 Size: 32 2799 32 2880 Fragments arranged by size: 2880 2799 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2498 2498 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 3068 1870 Size: 1198 4545 XmnI GAAnn'nnTTC Cuts at: 3872 3872 Size: 5743 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspXI PsrI SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI