(Circular) (Six-base) MAPSORT of: pFN21AB7146.seq Check: 5260 from: 1 to: 5743 pFN21AB7146 5743 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3553 278 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3086 1937 Size: 1149 4594 Acc65I G'GTAC_C Cuts at: 3064 3064 Size: 5743 AclI AA'CG_TT Cuts at: 1563 3870 4243 1563 Size: 2307 373 3063 Fragments arranged by size: 3063 2307 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3806 5190 5458 1632 Size: 2174 1384 268 1917 Fragments arranged by size: 2174 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5546 AflIII A'CryG_T Cuts at: 1203 4642 5619 1203 Size: 3439 977 1327 Fragments arranged by size: 3439 1327 977 AgeI A'CCGG_T Cuts at: 4574 4574 Size: 5743 AhdI GACnn_n'nnGTC Cuts at: 2061 4472 2061 Size: 2411 3332 AleI CACnn'nnGTG Cuts at: 1166 2082 1166 Size: 916 4827 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5743 AlwNI CAG_nnn'CTG Cuts at: 2368 3486 5058 2368 Size: 1118 1572 3053 Fragments arranged by size: 3053 1572 1118 ApaLI G'TGCA_C Cuts at: 3800 4956 3800 Size: 1156 4587 ApoI r'AATT_y Cuts at: 1493 2678 2819 3052 3278 3430 5539 1493 Size: 1185 141 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1185 233 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 4297 160 Size: 872 3265 1606 Fragments arranged by size: 3265 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2058 3068 1870 Size: 73 67 48 1010 4545 Fragments arranged by size: 4545 1010 73 67 48 BamHI G'GATC_C Cuts at: 3073 3440 3073 Size: 367 5376 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3064 4519 618 Size: 325 217 627 1277 1455 1842 Fragments arranged by size: 1842 1455 1277 627 325 217 BanII G_rGCy'C Cuts at: 1391 2736 2842 3062 1391 Size: 1345 106 220 4072 Fragments arranged by size: 4072 1345 220 106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2351 928 Size: 971 452 4320 Fragments arranged by size: 4320 971 452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3934 3968 1903 Size: 34 50 34 1913 34 3678 Fragments arranged by size: 3678 1913 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2128 3635 4845 2128 Size: 1507 1210 3026 Fragments arranged by size: 3026 1507 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5743 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4354 136 Size: 107 122 71 631 605 2682 1525 Fragments arranged by size: 2682 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2670 5738 2670 Size: 3068 2675 BlpI GC'TnA_GC Cuts at: 3160 3160 Size: 5743 Bme1580I G_kGCm'C Cuts at: 1790 2796 3804 4960 1790 Size: 1006 1008 1156 2573 Fragments arranged by size: 2573 1156 1008 1006 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5743 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2898 3474 4432 Size: 416 53 83 399 24 1481 576 958 Cuts at: 4432 442 Size: 1753 Fragments arranged by size: 1753 1481 958 576 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3027 1056 Size: 1971 3772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2798 2891 4403 1239 Size: 1559 93 1512 2579 Fragments arranged by size: 2579 1559 1512 93 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 3868 4733 5031 2174 Size: 1694 865 298 2886 Fragments arranged by size: 2886 1694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 4406 882 Size: 617 1116 1791 2219 Fragments arranged by size: 2219 1791 1116 617 BsaAI yAC'GTr Cuts at: 493 1204 2039 2180 3534 493 Size: 711 835 141 1354 2702 Fragments arranged by size: 2702 1354 835 711 141 BsaBI GATnn'nnATC Cuts at: 5737 5737 Size: 5743 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3550 3932 4567 275 Size: 53 83 186 936 2017 382 635 1451 Fragments arranged by size: 2017 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4176 4574 4848 4995 5406 4176 Size: 398 274 147 411 4513 Fragments arranged by size: 4513 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2477 2507 1178 Size: 30 165 30 1074 30 4414 Fragments arranged by size: 4414 1074 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2678 1186 Size: 1492 4251 BseYI C'CCAG_C Cuts at: 4946 5442 4946 Size: 496 5247 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 2381 2793 1163 Size: 573 600 45 412 4113 Fragments arranged by size: 4113 600 573 412 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3193 3954 4982 1540 Size: 275 186 1192 761 1028 2301 Fragments arranged by size: 2301 1192 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2089 2188 2209 3804 3889 4960 2089 Size: 99 21 1595 85 1071 2872 Fragments arranged by size: 2872 1595 1071 99 85 21 BsmI GAATG_Cn' Cuts at: 3254 3347 3254 Size: 93 5650 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2089 2188 2209 2736 2796 2842 3062 Size: 399 299 99 21 527 60 46 220 Cuts at: 3062 3804 3889 4960 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 527 399 299 220 99 85 60 46 21 BspEI T'CCGG_A Cuts at: 5406 5406 Size: 5743 BspHI T'CATG_A Cuts at: 2640 3632 5362 2640 Size: 992 1730 3021 Fragments arranged by size: 3021 1730 992 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3098 844 Size: 2254 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3630 1394 Size: 429 1807 3507 Fragments arranged by size: 3507 1807 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4238 4412 65 Size: 1183 2990 174 1396 Fragments arranged by size: 2990 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4387 4574 1700 Size: 110 2577 187 2869 Fragments arranged by size: 2869 2577 187 110 BsrGI T'GTAC_A Cuts at: 96 2379 96 Size: 2283 3460 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5743 BssSI C'ACGA_G Cuts at: 2583 3803 4815 2583 Size: 1220 1012 3511 Fragments arranged by size: 3511 1220 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5743 BstBI TT'CG_AA Cuts at: 4547 4547 Size: 5743 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5743 BstYI r'GATC_y Cuts at: 1972 2457 2557 2670 3073 3440 3825 3842 5294 Size: 485 100 113 403 367 385 17 1452 Cuts at: 5294 5380 5392 5738 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 485 403 385 367 346 113 100 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2994 513 Size: 552 1929 3262 Fragments arranged by size: 3262 1929 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5004 Fragments arranged by size: 5004 683 56 BtsI GCAGTG_nn' Cuts at: 2094 3259 4053 4073 2094 Size: 1165 794 20 3764 Fragments arranged by size: 3764 1165 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5743 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5708 DraI TTT'AAA Cuts at: 2508 3047 2508 Size: 539 5204 DraIII CAC_nnn'GTG Cuts at: 2183 2183 Size: 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 4750 809 Size: 507 1221 2213 1802 Fragments arranged by size: 2213 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708 Size: 54 1425 50 275 18 1360 889 1629 Cuts at: 5708 8 Size: 43 Fragments arranged by size: 1629 1425 1360 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3190 1537 Size: 275 1378 4090 Fragments arranged by size: 4090 1378 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2922 3037 3673 1353 Size: 662 907 115 636 3423 Fragments arranged by size: 3423 907 662 636 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4330 4704 4850 387 Size: 1064 138 2741 374 146 1280 Fragments arranged by size: 2741 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2798 2891 3806 4403 5190 5458 1239 Size: 393 1166 93 915 597 787 268 1524 Fragments arranged by size: 1524 1166 915 787 597 393 268 93 EcoO109I rG'GnC_Cy Cuts at: 2866 2866 Size: 5743 EcoRI G'AATT_C Cuts at: 2678 2819 3052 3430 2678 Size: 141 233 378 4991 Fragments arranged by size: 4991 378 233 141 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2305 2464 2734 2305 Size: 159 270 5314 Fragments arranged by size: 5314 270 159 FspI TGC'GCA Cuts at: 4249 4249 Size: 5743 HaeII r_GCGC'y Cuts at: 1678 4890 1678 Size: 3212 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2047 2059 2079 2091 2566 Size: 32 135 32 475 12 20 12 475 Cuts at: 2566 2598 4384 4416 4458 4490 1373 Size: 32 1786 32 42 32 2626 Fragments arranged by size: 2626 1786 475 475 135 42 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 3087 3333 669 Size: 988 281 1149 246 3079 Fragments arranged by size: 3079 1149 988 281 246 HindIII A'AGCT_T Cuts at: 748 2287 2469 2765 748 Size: 1539 182 296 3726 Fragments arranged by size: 3726 1539 296 182 HpaI GTT'AAC Cuts at: 3333 3333 Size: 5743 KpnI G_GTAC'C Cuts at: 3068 3068 Size: 5743 MfeI C'AATT_G Cuts at: 3342 3342 Size: 5743 MluI A'CGCG_T Cuts at: 5619 5619 Size: 5743 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4857 5041 1305 Size: 3552 184 2007 Fragments arranged by size: 3552 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3921 Fragments arranged by size: 3921 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2037 2082 2486 3701 4060 4219 518 Size: 648 871 45 404 1215 359 159 2042 Fragments arranged by size: 2042 1215 871 648 404 359 159 45 MspA1I CmG'CkG Cuts at: 1606 3159 3836 4984 5229 1606 Size: 1553 677 1148 245 2120 Fragments arranged by size: 2120 1553 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2994 513 Size: 552 1929 3262 Fragments arranged by size: 3262 1929 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5743 NheI G'CTAG_C Cuts at: 1052 3023 1052 Size: 1971 3772 NotI GC'GGCC_GC Cuts at: 3190 3190 Size: 5743 NspI r_CATG'y Cuts at: 1132 1229 3101 4646 1132 Size: 97 1872 1545 2229 Fragments arranged by size: 2229 1872 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5743 PciI A'CATG_T Cuts at: 4642 4642 Size: 5743 PflMI CCAn_nnn'nTGG Cuts at: 1108 2758 1108 Size: 1650 4093 PfoI T'CCnGG_A Cuts at: 2868 2868 Size: 5743 PmeI GTTT'AAAC Cuts at: 3047 3047 Size: 5743 PmlI CAC'GTG Cuts at: 2039 2039 Size: 5743 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3812 3844 5325 5357 3812 Size: 32 1481 32 4198 Fragments arranged by size: 4198 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2866 2866 Size: 5743 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5743 PsiI TTA'TAA Cuts at: 3313 3313 Size: 5743 PstI C_TGCA'G Cuts at: 830 2968 3095 830 Size: 2138 127 3478 Fragments arranged by size: 3478 2138 127 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5743 SalI G'TCGA_C Cuts at: 1936 3085 1936 Size: 1149 4594 SapI GCTCTTCn'nnn_ Cuts at: 3037 3037 Size: 5743 SbfI CC_TGCA'GG Cuts at: 3095 3095 Size: 5743 ScaI AGT'ACT Cuts at: 3991 5703 3991 Size: 1712 4031 SfcI C'TryA_G Cuts at: 826 1045 2226 2964 3091 4226 4907 5098 826 Size: 219 1181 738 127 1135 681 191 1471 Fragments arranged by size: 1471 1181 1135 738 681 219 191 127 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5743 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5743 SmaI CCC'GGG Cuts at: 1872 2060 3070 1872 Size: 188 1010 4545 Fragments arranged by size: 4545 1010 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 3847 4748 5010 820 Size: 197 926 246 1658 901 262 1553 Fragments arranged by size: 1658 1553 926 901 262 246 197 SnaBI TAC'GTA Cuts at: 493 3534 493 Size: 3041 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5743 SphI G_CATG'C Cuts at: 1132 3101 1132 Size: 1969 3774 SspI AAT'ATT Cuts at: 5 52 3667 5 Size: 47 3615 2081 Fragments arranged by size: 3615 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5743 StyI C'CwwG_G Cuts at: 513 1065 2526 2883 2994 3012 3018 3182 513 Size: 552 1461 357 111 18 6 164 3074 Fragments arranged by size: 3074 1461 552 357 164 111 18 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2765 3770 3787 3940 4125 4570 325 Size: 1255 1185 1005 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1185 1005 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 3989 5701 Size: 274 80 33 51 1443 402 1610 1712 Cuts at: 5701 96 Size: 138 Fragments arranged by size: 1712 1610 1443 402 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5703 5735 5703 Size: 32 5711 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5743 XbaI T'CTAG_A Cuts at: 3079 3079 Size: 5743 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5743 XmaI C'CCGG_G Cuts at: 1870 2058 3068 1870 Size: 188 1010 4545 Fragments arranged by size: 4545 1010 188 XmnI GAAnn'nnTTC Cuts at: 2447 2717 3007 3872 2447 Size: 270 290 865 4318 Fragments arranged by size: 4318 865 290 270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3551 276 Size: 53 83 186 936 2017 2468 Fragments arranged by size: 2468 2017 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI