Restriction map for
pFN21AB7133
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AflII AflIII AgeI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AspI AviII AvrII BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HpaI KasI
KspI MaeI MaeII MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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Acc65I 889 GGTACC G^GTACC
AccI 550 GTAGAC GT^[AC][GT]AC
AcsI 673 GAATTC [GA]^AATT[CT]
AhdI 63 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 82 CCTCAGG CC^T[ACGT]AGG
ApoI 673 GAATTC [GA]^AATT[CT]
Asp718I 889 GGTACC G^GTACC
AspEI 63 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 826 GGTCC G^G[AT]CC
BalI 875 TGGCCA TGG^CCA
BseRI 706 GAGGAG GAGGAG(10/8)
BsmFI 587 GGGAC GGGAC(10/14)
BsmI 170 GCATTC GAATGC(1/-1)
Bsp1407I 368 TGTACA T^GTACA
BspMI 783 GCAGGT ACCTGC(4/8)
BsrGI 368 TGTACA T^GTACA
BssSI 5 CTCGTG CACGAG(-5/-1)
BstXI 746 CCAAAGATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 82 CCTCAGG CC^T[ACGT]AGG
Cac8I 166 GCTGGC GC[ACGT]^[ACGT]GC
Eam1105I 63 GACCCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 82 CCTCAGG CC^T[ACGT]AGG
EcoRI 673 GAATTC G^AATTC
FauI 220 GCGGG CCCGC(4/6)
HincII 511 GTCAAC GT[CT]^[GA]AC
HindII 511 GTCAAC GT[CT]^[GA]AC
KpnI 889 GGTACC GGTAC^C
MluNI 875 TGGCCA TGG^CCA
MscI 875 TGGCCA TGG^CCA
MseI 637 TTAA T^TAA
MslI 262 CACCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 206 CCGCTG C[AC]G^C[GT]G
SfaNI 612 GATGC GCATC(5/9)
SmaI 65 CCCGGG CCC^GGG
SspBI 368 TGTACA T^GTACA
StuI 293 AGGCCT AGG^CCT
TaqI 542 TCGA T^CGA
TfiI 568 GAATC G^A[AT]TC
Tru9I 637 TTAA T^TAA
Tsp45I 88 GTCAC ^GT[GC]AC
TthHB8I 542 TCGA T^CGA
XmaCI 65 CCCGGG C^CCGGG
XmaI 65 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 439 GAGCAC G[AT]GC[AT]^C
709 GAGCAC
AvaI 53 CCCGAG C^[CT]CG[GA]G
65 CCCGGG
BanII 191 GAGCCC G[GA]GC[CT]^C
312 GAGCCC
BcnI 65 CCCGG CC^[GC]GG
66 CCGGG
BglII 665 AGATCT A^GATCT
750 AGATCT
BpmI 772 CTGGAG CTGGAG(16/14)
865 CTGGAG
BsaI 61 GAGACC GGTCTC(1/5)
70 GGTCTC
BsgI 355 GTGCAG GTGCAG(16/14)
757 GTGCAG
BsiHKAI 439 GAGCAC G[AT]GC[AT]^C
709 GAGCAC
BsiYI 160 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
616 CCTCTCCTGGG
BslI 160 CCTGCTGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
616 CCTCTCCTGGG
BsmAI 61 GAGAC GTCTC(1/5)
71 GTCTC
BsoBI 53 CCCGAG C^[CT]CG[GA]G
65 CCCGGG
BstF5I 230 CATCC GGATG(2/0)
302 CATCC
DraII 110 AGGCCCC [GA]G^G[ACGT]CC[CT]
473 AGGGCCT
EaeI 522 CGGCCA [CT]^GGCC[GA]
875 TGGCCA
EcoO109I 110 AGGCCCC [GA]G^G[ACGT]CC[CT]
473 AGGGCCT
EcoT14I 264 CCAAGG C^C[AT][AT]GG
878 CCAAGG
FokI 230 CATCC GGATG(9/13)
302 CATCC
HapII 66 CCGG C^CGG
950 CCGG
HindIII 604 AAGCTT A^AGCTT
926 AAGCTT
HpaII 66 CCGG C^CGG
950 CCGG
HphI 268 GGTGA GGTGA(8/7)
743 TCACC
MaeIII 88 GTCAC ^GT[ACGT]AC
394 GTTAC
MspI 66 CCGG C^CGG
950 CCGG
NciI 65 CCCGG CC^[GC]GG
66 CCGGG
PleI 692 GAGTC GAGTC(4/5)
953 GAGTC
SmlI 457 CTCAAG C^T[CT][GA]AG
496 CTTGAG
StyI 264 CCAAGG C^C[AT][AT]GG
878 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BanI BbvI BmyI BsaJI Bsp1286I
BssKI BstNI BstYI Cfr13I DdeI
DpnII EarI EcoRII Fnu4HI HaeIII
HinfI ItaI Ksp632I MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIV RsaI Sau3AI Sau96I ScrFI
TseI Tsp509I TspRI XhoII