Restriction map for
pFN21AB7133
(Circular) (Six-base) MAPSORT of: pFN21AB7133.seq Check: 4213 from: 1 to: 5662
pFN21AB7133 5662 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2779 2779
Size: 5662
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3472 278
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2556 3005 1937
Size: 619 449 4594
Fragments arranged by size:
4594 619 449
Acc65I G'GTAC_C
Cuts at: 2894 2983 2894
Size: 89 5573
AclI AA'CG_TT
Cuts at: 1563 3789 4162 1563
Size: 2226 373 3063
Fragments arranged by size:
3063 2226 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2118 3725 5109 5377 1632
Size: 486 1607 1384 268 1917
Fragments arranged by size:
1917 1607 1384 486 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5465
AflIII A'CryG_T
Cuts at: 1203 4561 5538 1203
Size: 3358 977 1327
Fragments arranged by size:
3358 1327 977
AgeI A'CCGG_T
Cuts at: 4493 4493
Size: 5662
AhdI GACnn_n'nnGTC
Cuts at: 2073 4391 2073
Size: 2318 3344
AleI CACnn'nnGTG
Cuts at: 1166 2271 1166
Size: 1105 4557
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5662
AlwNI CAG_nnn'CTG
Cuts at: 2368 2589 2660 3405 4977 2368
Size: 221 71 745 1572 3053
Fragments arranged by size:
3053 1572 745 221 71
ApaLI G'TGCA_C
Cuts at: 3719 4875 3719
Size: 1156 4506
ApoI r'AATT_y
Cuts at: 1493 2678 2971 3197 3349 5458 1493
Size: 1185 293 226 152 2109 1697
Fragments arranged by size:
2109 1697 1185 293 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4216 160
Size: 872 3184 1606
Fragments arranged by size:
3184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
AvaI C'yCGr_G
Cuts at: 1870 1943 2058 2070 2987 1870
Size: 73 115 12 917 4545
Fragments arranged by size:
4545 917 115 73 12
BamHI G'GATC_C
Cuts at: 2992 3359 2992
Size: 367 5295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2255 2791 2894 2983 4438
Size: 325 217 627 468 536 103 89 1455
Cuts at: 4438 618
Size: 1842
Fragments arranged by size:
1842 1455 627 536 468 325 217 103
89
BanII G_rGCy'C
Cuts at: 1391 2200 2321 2981 1391
Size: 809 121 660 4072
Fragments arranged by size:
4072 809 660 121
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4691
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3853 3887 1903
Size: 34 50 34 1832 34 3678
Fragments arranged by size:
3678 1832 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3554 4764 3554
Size: 1210 4452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4273 136
Size: 107 122 71 631 605 2601 1525
Fragments arranged by size:
2601 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2670 2755 5657 2670
Size: 85 2902 2675
Fragments arranged by size:
2902 2675 85
BlpI GC'TnA_GC
Cuts at: 3079 3079
Size: 5662
Bme1580I G_kGCm'C
Cuts at: 1790 2038 2796 3723 4879 1790
Size: 248 758 927 1156 2573
Fragments arranged by size:
2573 1156 927 758 248
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3393 4351 442
Size: 416 53 83 399 24 1976 958 1753
Fragments arranged by size:
1976 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5662
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2734 2734
Size: 5662
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 2891 4322 1239
Size: 1559 93 1431 2579
Fragments arranged by size:
2579 1559 1431 93
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2447 2522 3787 4652 4950 2447
Size: 75 1265 865 298 3159
Fragments arranged by size:
3159 1265 865 298 75
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2060 2081 4325 882
Size: 617 561 21 2244 2219
Fragments arranged by size:
2244 2219 617 561 21
BsaAI yAC'GTr
Cuts at: 493 1204 3453 493
Size: 711 2249 2702
Fragments arranged by size:
2702 2249 711
BsaBI GATnn'nnATC
Cuts at: 5656 5656
Size: 5662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3469 3851 4486 275
Size: 53 83 186 936 1936 382 635 1451
Fragments arranged by size:
1936 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4095 4493 4767 4914 5325 4095
Size: 398 274 147 411 4432
Fragments arranged by size:
4432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5437
Fragments arranged by size:
5437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2726 1186
Size: 1540 4122
BseYI C'CCAG_C
Cuts at: 2795 4865 5361 2795
Size: 2070 496 3096
Fragments arranged by size:
3096 2070 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2381 2783 1163
Size: 573 645 402 4042
Fragments arranged by size:
4042 645 573 402
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3112 3873 4901 1540
Size: 275 186 1111 761 1028 2301
Fragments arranged by size:
2301 1111 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2448 2718 3723 3808 4879 2448
Size: 270 1005 85 1071 3231
Fragments arranged by size:
3231 1071 1005 270 85
BsmI GAATG_Cn'
Cuts at: 2175 3173 3266 2175
Size: 998 93 4571
Fragments arranged by size:
4571 998 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2038 2200 2321 2448 2718 2796 2981
Size: 399 248 162 121 127 270 78 185
Cuts at: 2981 3723 3808 4879 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 270 248 185 162
127 121 85 78
BspEI T'CCGG_A
Cuts at: 5325 5325
Size: 5662
BspHI T'CATG_A
Cuts at: 3551 5281 3551
Size: 1730 3932
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2779 3017 844
Size: 1935 238 3489
Fragments arranged by size:
3489 1935 238
BsrBI CCG'CTC
Cuts at: 1394 1823 3549 1394
Size: 429 1726 3507
Fragments arranged by size:
3507 1726 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4157 4331 65
Size: 1183 2909 174 1396
Fragments arranged by size:
2909 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4306 4493 1700
Size: 110 2496 187 2869
Fragments arranged by size:
2869 2496 187 110
BsrGI T'GTAC_A
Cuts at: 96 2373 96
Size: 2277 3385
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5662
BssSI C'ACGA_G
Cuts at: 2010 3722 4734 2010
Size: 1712 1012 2938
Fragments arranged by size:
2938 1712 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5662
BstBI TT'CG_AA
Cuts at: 4466 4466
Size: 5662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5662
BstXI CCAn_nnnn'nTGG
Cuts at: 2758 2758
Size: 5662
BstYI r'GATC_y
Cuts at: 1972 2329 2457 2670 2755 2992 3359 3744 3761
Size: 357 128 213 85 237 367 385 17
Cuts at: 3761 5213 5299 5311 5657 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 357 346 237 213
128 86 85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2088 2088
Size: 5662
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5110
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4923
Fragments arranged by size:
4923 683 56
BtsI GCAGTG_nn'
Cuts at: 2205 2806 3178 3972 3992 2205
Size: 601 372 794 20 3875
Fragments arranged by size:
3875 794 601 372 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2807 2842 529
Size: 35 2243 35 3349
Fragments arranged by size:
3349 2243 35 35
DraI TTT'AAA
Cuts at: 2966 2966
Size: 5662
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4669 809
Size: 507 3353 1802
Fragments arranged by size:
3353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2527 2880 3109
Size: 54 1425 50 275 18 697 353 229
Cuts at: 3109 3998 5627 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 697 353 275 229 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3109 1537
Size: 275 1297 4090
Fragments arranged by size:
4090 1297 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2015 2106 2137 2661 2940 3592 1353
Size: 662 91 31 524 279 652 3423
Fragments arranged by size:
3423 662 652 524 279 91 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4249 4623 4769 387
Size: 1064 138 2660 374 146 1280
Fragments arranged by size:
2660 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2118 2798 2891 3725 4322 5109 5377
Size: 393 486 680 93 834 597 787 268
Cuts at: 5377 1239
Size: 1524
Fragments arranged by size:
1524 834 787 680 597 486 393 268
93
EcoO109I rG'GnC_Cy
Cuts at: 2116 2479 2116
Size: 363 5299
EcoRI G'AATT_C
Cuts at: 2678 2971 3349 2678
Size: 293 378 4991
Fragments arranged by size:
4991 378 293
FspI TGC'GCA
Cuts at: 4168 4168
Size: 5662
HaeII r_GCGC'y
Cuts at: 1678 4809 1678
Size: 3131 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2059 2091 2558 2590 4303
Size: 32 135 32 487 32 467 32 1713
Cuts at: 4303 4335 4377 4409 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1713 487 467 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2518 3006 3252 669
Size: 988 281 580 488 246 3079
Fragments arranged by size:
3079 988 580 488 281 246
HindIII A'AGCT_T
Cuts at: 748 2609 2931 748
Size: 1861 322 3479
Fragments arranged by size:
3479 1861 322
HpaI GTT'AAC
Cuts at: 3252 3252
Size: 5662
KpnI G_GTAC'C
Cuts at: 2898 2987 2898
Size: 89 5573
MfeI C'AATT_G
Cuts at: 3261 3261
Size: 5662
MluI A'CGCG_T
Cuts at: 5538 5538
Size: 5662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4776 4960 1305
Size: 3471 184 2007
Fragments arranged by size:
3471 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2882 10
Size: 54 1425 343 1050 2790
Fragments arranged by size:
2790 1425 1050 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2271 3620 3979 4138 518
Size: 648 1105 1349 359 159 2042
Fragments arranged by size:
2042 1349 1105 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2213 3078 3755 4903 5148 1606
Size: 607 865 677 1148 245 2120
Fragments arranged by size:
2120 1148 865 677 607 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5110
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5662
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5662
NotI GC'GGCC_GC
Cuts at: 3109 3109
Size: 5662
NspI r_CATG'y
Cuts at: 1132 1229 3020 4565 1132
Size: 97 1791 1545 2229
Fragments arranged by size:
2229 1791 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5662
PciI A'CATG_T
Cuts at: 4561 4561
Size: 5662
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5662
PfoI T'CCnGG_A
Cuts at: 2868 2868
Size: 5662
PmeI GTTT'AAAC
Cuts at: 2966 2966
Size: 5662
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3731 3763 5244 5276 3731
Size: 32 1481 32 4117
Fragments arranged by size:
4117 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5662
PsiI TTA'TAA
Cuts at: 3232 3232
Size: 5662
PstI C_TGCA'G
Cuts at: 830 3014 830
Size: 2184 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5662
SalI G'TCGA_C
Cuts at: 1936 3004 1936
Size: 1068 4594
SbfI CC_TGCA'GG
Cuts at: 3014 3014
Size: 5662
ScaI AGT'ACT
Cuts at: 3910 5622 3910
Size: 1712 3950
SfcI C'TryA_G
Cuts at: 826 1045 3010 4145 4826 5017 826
Size: 219 1965 1135 681 191 1471
Fragments arranged by size:
1965 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5662
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5662
SmaI CCC'GGG
Cuts at: 1872 2072 2989 1872
Size: 200 917 4545
Fragments arranged by size:
4545 917 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2462 2501 3766 4667 4929 820
Size: 197 926 519 39 1265 901 262 1553
Fragments arranged by size:
1553 1265 926 901 519 262 197 39
SnaBI TAC'GTA
Cuts at: 493 3453 493
Size: 2960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5662
SphI G_CATG'C
Cuts at: 1132 3020 1132
Size: 1888 3774
SspI AAT'ATT
Cuts at: 5 52 3586 5
Size: 47 3534 2081
Fragments arranged by size:
3534 2081 47
StuI AGG'CCT
Cuts at: 1517 2300 1517
Size: 783 4879
StyI C'CwwG_G
Cuts at: 513 1065 2269 2883 3101 513
Size: 552 1204 614 218 3074
Fragments arranged by size:
3074 1204 614 552 218
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2618 3689 3706 3859 4044 4489 325
Size: 1255 1038 1071 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1071 1038 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2373 2781 3908
Size: 274 80 33 51 1443 396 408 1127
Cuts at: 3908 5620 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1127 408 396 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5622 5654 5622
Size: 32 5630
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5662
XbaI T'CTAG_A
Cuts at: 2998 2998
Size: 5662
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5662
XmaI C'CCGG_G
Cuts at: 1870 2070 2987 1870
Size: 200 917 4545
Fragments arranged by size:
4545 917 200
XmnI GAAnn'nnTTC
Cuts at: 3791 3791
Size: 5662
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3470 276
Size: 53 83 186 936 1936 2468
Fragments arranged by size:
2468 1936 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI