Restriction map for
pFN21AB7133
(Linear) (Six-base) MAPSORT of: KIBB7133.seq Check: 2927 from: 1 to: 957
KIBB7133.orf
With 182 enzymes: SgfI *
March 3, 2008 10:59 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 774 957
Size: 774 183
AccI GT'mk_AC
Cuts at: 0 551 957
Size: 551 406
Acc65I G'GTAC_C
Cuts at: 0 889 957
Size: 889 68
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 957
Size: 113 844
AhdI GACnn_n'nnGTC
Cuts at: 0 68 957
Size: 68 889
AleI CACnn'nnGTG
Cuts at: 0 266 957
Size: 266 691
AlwNI CAG_nnn'CTG
Cuts at: 0 363 584 655 957
Size: 363 221 71 302
Fragments arranged by size:
363 302 221 71
ApoI r'AATT_y
Cuts at: 0 673 957
Size: 673 284
AvaI C'yCGr_G
Cuts at: 0 53 65 957
Size: 53 12 892
Fragments arranged by size:
892 53 12
BanI G'GyrC_C
Cuts at: 0 250 786 889 957
Size: 250 536 103 68
Fragments arranged by size:
536 250 103 68
BanII G_rGCy'C
Cuts at: 0 195 316 957
Size: 195 121 641
Fragments arranged by size:
641 195 121
BglII A'GATC_T
Cuts at: 0 665 750 957
Size: 665 85 207
Fragments arranged by size:
665 207 85
Bme1580I G_kGCm'C
Cuts at: 0 33 791 957
Size: 33 758 166
Fragments arranged by size:
758 166 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 729 957
Size: 729 228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 886 957
Size: 793 93 71
Fragments arranged by size:
793 93 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 442 517 957
Size: 442 75 440
Fragments arranged by size:
442 440 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 55 76 957
Size: 55 21 881
Fragments arranged by size:
881 55 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 721 957
Size: 721 236
BseYI C'CCAG_C
Cuts at: 0 790 957
Size: 790 167
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 778 957
Size: 376 402 179
Fragments arranged by size:
402 376 179
BsiHKAI G_wGCw'C
Cuts at: 0 443 713 957
Size: 443 270 244
Fragments arranged by size:
443 270 244
BsmI GAATG_Cn'
Cuts at: 0 170 957
Size: 170 787
Bsp1286I G_dGCh'C
Cuts at: 0 33 195 316 443 713 791 957
Size: 33 162 121 127 270 78 166
Fragments arranged by size:
270 166 162 127 121 78 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 774 957
Size: 774 183
BsrGI T'GTAC_A
Cuts at: 0 368 957
Size: 368 589
BssSI C'ACGA_G
Cuts at: 0 5 957
Size: 5 952
BstXI CCAn_nnnn'nTGG
Cuts at: 0 753 957
Size: 753 204
BstYI r'GATC_y
Cuts at: 0 324 452 665 750 957
Size: 324 128 213 85 207
Fragments arranged by size:
324 213 207 128 85
Bsu36I CC'TnA_GG
Cuts at: 0 83 957
Size: 83 874
BtsI GCAGTG_nn'
Cuts at: 0 200 801 957
Size: 200 601 156
Fragments arranged by size:
601 200 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 802 837 957
Size: 802 35 120
Fragments arranged by size:
802 120 35
EaeI y'GGCC_r
Cuts at: 0 522 875 957
Size: 522 353 82
Fragments arranged by size:
522 353 82
EarI CTCTTCn'nnn_
Cuts at: 0 10 101 132 656 935 957
Size: 10 91 31 524 279 22
Fragments arranged by size:
524 279 91 31 22 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 793 886 957
Size: 113 680 93 71
Fragments arranged by size:
680 113 93 71
EcoO109I rG'GnC_Cy
Cuts at: 0 111 474 957
Size: 111 363 483
Fragments arranged by size:
483 363 111
EcoRI G'AATT_C
Cuts at: 0 673 957
Size: 673 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 54 86 553 585 957
Size: 54 32 467 32 372
Fragments arranged by size:
467 372 54 32 32
HincII GTy'rAC
Cuts at: 0 513 957
Size: 513 444
HindIII A'AGCT_T
Cuts at: 0 604 926 957
Size: 604 322 31
Fragments arranged by size:
604 322 31
KpnI G_GTAC'C
Cuts at: 0 893 957
Size: 893 64
MscI TGG'CCA
Cuts at: 0 877 957
Size: 877 80
MslI CAynn'nnrTG
Cuts at: 0 266 957
Size: 266 691
MspA1I CmG'CkG
Cuts at: 0 208 957
Size: 208 749
PfoI T'CCnGG_A
Cuts at: 0 863 957
Size: 863 94
SmaI CCC'GGG
Cuts at: 0 67 957
Size: 67 890
SmlI C'TyrA_G
Cuts at: 0 457 496 957
Size: 457 39 461
Fragments arranged by size:
461 457 39
StuI AGG'CCT
Cuts at: 0 295 957
Size: 295 662
StyI C'CwwG_G
Cuts at: 0 264 878 957
Size: 264 614 79
Fragments arranged by size:
614 264 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 613 957
Size: 613 344
TatI w'GTAC_w
Cuts at: 0 368 776 957
Size: 368 408 181
Fragments arranged by size:
408 368 181
XmaI C'CCGG_G
Cuts at: 0 65 957
Size: 65 892
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlwNI ApoI
AvaI BanI BanII BglII Bme1580I BplI BpmI BpuEI
BsaI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrGI BssSI BstXI BstYI Bsu36I BtsI CspCI EaeI
EarI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII KpnI
MscI MslI MspA1I PfoI SmaI SmlI StuI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmrI BmtI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstZ17I BtgI
BtgZI ClaI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI