(Circular) (Six-base) MAPSORT of: pFN21AB7132.seq Check: 339 from: 1 to: 5659 pFN21AB7132 5659 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2479 2561 2479 Size: 82 5577 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3469 278 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3002 1937 Size: 1065 4594 Acc65I G'GTAC_C Cuts at: 2980 2980 Size: 5659 AclI AA'CG_TT Cuts at: 1563 3786 4159 1563 Size: 2223 373 3063 Fragments arranged by size: 3063 2223 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2036 2449 3722 5106 5374 1632 Size: 404 413 1273 1384 268 1917 Fragments arranged by size: 1917 1384 1273 413 404 268 AfeI AGC'GCT Cuts at: 2030 2030 Size: 5659 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5462 AflIII A'CryG_T Cuts at: 1203 4558 5535 1203 Size: 3355 977 1327 Fragments arranged by size: 3355 1327 977 AgeI A'CCGG_T Cuts at: 4490 4490 Size: 5659 AhdI GACnn_n'nnGTC Cuts at: 4388 4388 Size: 5659 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5659 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2133 1184 Size: 949 4710 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 2309 Size: 32 5627 AlwNI CAG_nnn'CTG Cuts at: 3402 4974 3402 Size: 1572 4087 ApaI G_GGCC'C Cuts at: 2845 2845 Size: 5659 ApaLI G'TGCA_C Cuts at: 3716 4872 3716 Size: 1156 4503 ApoI r'AATT_y Cuts at: 1493 2968 3194 3346 5455 1493 Size: 1475 226 152 2109 1697 Fragments arranged by size: 2109 1697 1475 226 152 AseI AT'TA_AT Cuts at: 160 1032 4213 160 Size: 872 3181 1606 Fragments arranged by size: 3181 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 AvaI C'yCGr_G Cuts at: 1870 1943 2984 1870 Size: 73 1041 4545 Fragments arranged by size: 4545 1041 73 BamHI G'GATC_C Cuts at: 2989 3356 2989 Size: 367 5292 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2068 2204 2794 2980 4435 Size: 325 217 627 281 136 590 186 1455 Cuts at: 4435 618 Size: 1842 Fragments arranged by size: 1842 1455 627 590 325 281 217 186 136 BanII G_rGCy'C Cuts at: 1391 2845 2978 1391 Size: 1454 133 4072 Fragments arranged by size: 4072 1454 133 BbeI G_GCGC'C Cuts at: 2798 2798 Size: 5659 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4688 BbvCI CC'TCA_GC Cuts at: 2040 2103 2040 Size: 63 5596 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3850 3884 1903 Size: 34 50 34 1829 34 3678 Fragments arranged by size: 3678 1829 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3551 4761 3551 Size: 1210 4449 BclI T'GATC_A Cuts at: 1548 2139 2590 1548 Size: 591 451 4617 Fragments arranged by size: 4617 591 451 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4270 136 Size: 107 122 71 631 605 2598 1525 Fragments arranged by size: 2598 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5654 5654 Size: 5659 BlpI GC'TnA_GC Cuts at: 3076 3076 Size: 5659 Bme1580I G_kGCm'C Cuts at: 1790 2845 3720 4876 1790 Size: 1055 875 1156 2573 Fragments arranged by size: 2573 1156 1055 875 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5659 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3390 4348 442 Size: 416 53 83 399 24 1973 958 1753 Fragments arranged by size: 1973 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5659 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2224 2224 Size: 5659 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2801 2894 4319 1239 Size: 1097 465 93 1425 2579 Fragments arranged by size: 2579 1425 1097 465 93 Bpu10I CC'TnA_GC Cuts at: 2040 2103 2738 2040 Size: 63 635 4961 Fragments arranged by size: 4961 635 63 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2174 2441 3784 4649 4947 2174 Size: 267 1343 865 298 2886 Fragments arranged by size: 2886 1343 865 298 267 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4322 882 Size: 617 2823 2219 Fragments arranged by size: 2823 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3450 493 Size: 711 2246 2702 Fragments arranged by size: 2702 2246 711 BsaBI GATnn'nnATC Cuts at: 5653 5653 Size: 5659 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2795 3466 3848 4483 Size: 53 83 186 936 1262 671 382 635 Cuts at: 4483 275 Size: 1451 Fragments arranged by size: 1451 1262 936 671 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4092 4490 4764 4911 5322 4092 Size: 398 274 147 411 4429 Fragments arranged by size: 4429 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2309 2339 1178 Size: 30 165 30 906 30 4498 Fragments arranged by size: 4498 906 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2116 2119 2120 2171 2200 2294 2681 2960 Size: 930 3 1 51 29 94 387 279 Cuts at: 2960 1186 Size: 3885 Fragments arranged by size: 3885 930 387 279 94 51 29 3 1 BseYI C'CCAG_C Cuts at: 2305 2581 4862 5358 2305 Size: 276 2281 496 2606 Fragments arranged by size: 2606 2281 496 276 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2786 1163 Size: 573 372 678 4036 Fragments arranged by size: 4036 678 573 372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3109 3870 4898 1540 Size: 275 186 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2721 3720 3805 4876 2721 Size: 999 85 1071 3504 Fragments arranged by size: 3504 1071 999 85 BsmI GAATG_Cn' Cuts at: 3170 3263 3170 Size: 93 5566 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2721 2845 2978 3720 3805 4876 1391 Size: 399 931 124 133 742 85 1071 2174 Fragments arranged by size: 2174 1071 931 742 399 133 124 85 BspEI T'CCGG_A Cuts at: 5322 5322 Size: 5659 BspHI T'CATG_A Cuts at: 3548 5278 3548 Size: 1730 3929 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2479 2561 3014 844 Size: 1635 82 453 3489 Fragments arranged by size: 3489 1635 453 82 BsrBI CCG'CTC Cuts at: 1394 1823 3546 1394 Size: 429 1723 3507 Fragments arranged by size: 3507 1723 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2278 4154 4328 65 Size: 1183 1030 1876 174 1396 Fragments arranged by size: 1876 1396 1183 1030 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2398 2528 2609 4303 4490 1700 Size: 110 588 130 81 1694 187 2869 Fragments arranged by size: 2869 1694 588 187 130 110 81 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5659 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5659 BssSI C'ACGA_G Cuts at: 3719 4731 3719 Size: 1012 4647 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5659 BstBI TT'CG_AA Cuts at: 4463 4463 Size: 5659 BstEII G'GTnAC_C Cuts at: 1184 2175 1184 Size: 991 4668 BstYI r'GATC_y Cuts at: 1972 2989 3356 3741 3758 5210 5296 5308 5654 Size: 1017 367 385 17 1452 86 12 346 Cuts at: 5654 1972 Size: 1977 Fragments arranged by size: 1977 1452 1017 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2199 2199 Size: 5659 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5107 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4920 Fragments arranged by size: 4920 683 56 BtsI GCAGTG_nn' Cuts at: 2809 3175 3969 3989 2809 Size: 366 794 20 4479 Fragments arranged by size: 4479 794 366 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5659 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5624 DraI TTT'AAA Cuts at: 2963 2963 Size: 5659 DraIII CAC_nnn'GTG Cuts at: 2241 2241 Size: 5659 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4666 809 Size: 507 3350 1802 Fragments arranged by size: 3350 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2530 3106 3995 Size: 54 1425 50 275 18 700 576 889 Cuts at: 3995 5624 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 700 576 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3106 1537 Size: 275 1294 4090 Fragments arranged by size: 4090 1294 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2015 2218 2271 2925 3589 1353 Size: 662 203 53 654 664 3423 Fragments arranged by size: 3423 664 662 654 203 53 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4246 4620 4766 387 Size: 1064 138 2657 374 146 1280 Fragments arranged by size: 2657 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2336 2449 2801 2894 3722 4319 Size: 393 404 300 113 352 93 828 597 Cuts at: 4319 5106 5374 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 597 404 393 352 300 268 113 93 EcoNI CCTnn'n_nnAGG Cuts at: 2156 2156 Size: 5659 EcoO109I rG'GnC_Cy Cuts at: 2841 2842 2841 Size: 1 5658 EcoRI G'AATT_C Cuts at: 2968 3346 2968 Size: 378 5281 EcoRV GAT'ATC Cuts at: 2461 2461 Size: 5659 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 5659 FspI TGC'GCA Cuts at: 4165 4165 Size: 5659 HaeII r_GCGC'y Cuts at: 1678 2032 2798 4806 1678 Size: 354 766 2008 2531 Fragments arranged by size: 2531 2008 766 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2159 2191 4300 4332 4374 Size: 32 135 32 587 32 2109 32 42 Cuts at: 4374 4406 1373 Size: 32 2626 Fragments arranged by size: 2626 2109 587 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3003 3249 669 Size: 988 281 1065 246 3079 Fragments arranged by size: 3079 1065 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5659 HpaI GTT'AAC Cuts at: 3249 3249 Size: 5659 KasI G'GCGC_C Cuts at: 2794 2794 Size: 5659 KpnI G_GTAC'C Cuts at: 2984 2984 Size: 5659 MfeI C'AATT_G Cuts at: 3258 3258 Size: 5659 MluI A'CGCG_T Cuts at: 5535 5535 Size: 5659 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4773 4957 1305 Size: 3468 184 2007 Fragments arranged by size: 3468 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3837 Fragments arranged by size: 3837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2507 3617 3976 4135 518 Size: 648 1323 18 1110 359 159 2042 Fragments arranged by size: 2042 1323 1110 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2102 3075 3752 4900 5145 1606 Size: 496 973 677 1148 245 2120 Fragments arranged by size: 2120 1148 973 677 496 245 NaeI GCC'GGC Cuts at: 2530 2530 Size: 5659 NarI GG'CG_CC Cuts at: 2795 2795 Size: 5659 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5107 NdeI CA'TA_TG Cuts at: 387 387 Size: 5659 NgoMIV G'CCGG_C Cuts at: 2528 2528 Size: 5659 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5659 NotI GC'GGCC_GC Cuts at: 3106 3106 Size: 5659 NspI r_CATG'y Cuts at: 1132 1229 2488 2882 3017 4562 1132 Size: 97 1259 394 135 1545 2229 Fragments arranged by size: 2229 1545 1259 394 135 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5659 PciI A'CATG_T Cuts at: 4558 4558 Size: 5659 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5659 PfoI T'CCnGG_A Cuts at: 2328 2871 2328 Size: 543 5116 PmeI GTTT'AAAC Cuts at: 2963 2963 Size: 5659 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2309 2341 3728 3760 5241 5273 2309 Size: 32 1387 32 1481 32 2695 Fragments arranged by size: 2695 1481 1387 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5659 PsiI TTA'TAA Cuts at: 3229 3229 Size: 5659 PspOMI G'GGCC_C Cuts at: 2841 2841 Size: 5659 PstI C_TGCA'G Cuts at: 830 3011 830 Size: 2181 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5659 PvuII CAG'CTG Cuts at: 2102 2102 Size: 5659 SalI G'TCGA_C Cuts at: 1936 3001 1936 Size: 1065 4594 SbfI CC_TGCA'GG Cuts at: 3011 3011 Size: 5659 ScaI AGT'ACT Cuts at: 3907 5619 3907 Size: 1712 3947 SfcI C'TryA_G Cuts at: 826 1045 3007 4142 4823 5014 826 Size: 219 1962 1135 681 191 1471 Fragments arranged by size: 1962 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2796 2796 Size: 5659 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5659 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5659 SmaI CCC'GGG Cuts at: 1872 2986 1872 Size: 1114 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2420 3763 4664 4926 820 Size: 197 926 210 267 1343 901 262 1553 Fragments arranged by size: 1553 1343 926 901 267 262 210 197 SnaBI TAC'GTA Cuts at: 493 3450 493 Size: 2957 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5659 SphI G_CATG'C Cuts at: 1132 2488 3017 1132 Size: 1356 529 3774 Fragments arranged by size: 3774 1356 529 SspI AAT'ATT Cuts at: 5 52 3583 5 Size: 47 3531 2081 Fragments arranged by size: 3531 2081 47 StuI AGG'CCT Cuts at: 1517 2049 1517 Size: 532 5127 StyI C'CwwG_G Cuts at: 513 1065 2059 2837 3098 513 Size: 552 994 778 261 3074 Fragments arranged by size: 3074 994 778 552 261 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2573 2752 2768 3686 3703 3856 4041 Size: 1255 993 179 16 918 17 153 185 Cuts at: 4041 4486 325 Size: 445 1498 Fragments arranged by size: 1498 1255 993 918 445 185 179 153 17 16 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3905 5617 96 Size: 274 80 33 51 1443 1928 1712 138 Fragments arranged by size: 1928 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2303 2335 5619 5651 2303 Size: 32 3284 32 2311 Fragments arranged by size: 3284 2311 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5659 XbaI T'CTAG_A Cuts at: 2995 2995 Size: 5659 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5659 XmaI C'CCGG_G Cuts at: 1870 2984 1870 Size: 1114 4545 XmnI GAAnn'nnTTC Cuts at: 2049 3788 2049 Size: 1739 3920 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3467 276 Size: 53 83 186 936 1933 2468 Fragments arranged by size: 2468 1933 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI