Restriction map for
pFN21AB7130
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BamHI BanI BanII
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BseAI BseRI BsiEI BsiHKAI
BsiWI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspLU11I
BspMI BsrDI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstF5I
BstPI BstUI BstZ17I Cac8I CelII
Cfr10I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV FauI
FbaI FokI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AfaI 847 GTAC GT^AC
AlwI 121 GGATC GGATC(4/5)
AocI 870 CCTTAGG CC^T[ACGT]AGG
Asp700I 185 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 818 TGGCCA TGG^CCA
BfaI 277 CTAG C^TAG
BsaWI 809 ACCGGA [AT]^CCGG[AT]
BsmI 468 GCATTC GAATGC(1/-1)
BsrBI 541 CCGCTC CCGCTC(-3/-3)
BssSI 247 CTCGTG CACGAG(-5/-1)
BstXI 329 CCATTTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 121 GGATCT [GA]^GATC[CT]
Bsu36I 870 CCTTAGG CC^T[ACGT]AGG
CfoI 833 GCGC GCG^C
DpnII 122 GATC ^GATC
DraI 925 TTTAAA TTT^AAA
DraII 682 AGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 614 CCATGG C^C[GA][CT]GG
EaeI 818 TGGCCA [CT]^GGCC[GA]
Eco81I 870 CCTTAGG CC^T[ACGT]AGG
EcoO109I 682 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 614 CCATGG C^C[AT][AT]GG
EcoT22I 466 ATGCAT ATGCA^T
HaeII 832 AGCGCC [GA]GCGC^[CT]
HapII 810 CCGG C^CGG
HhaI 833 GCGC GCG^C
HinP1I 833 GCGC G^CGC
HpaII 810 CCGG C^CGG
MaeI 277 CTAG C^TAG
MboI 122 GATC ^GATC
MflI 121 GGATCT [GA]^GATC[CT]
MluNI 818 TGGCCA TGG^CCA
MscI 818 TGGCCA TGG^CCA
MspA1I 428 CAGCTG C[AC]G^C[GT]G
MspI 810 CCGG C^CGG
NcoI 614 CCATGG C^CATGG
NdeII 122 GATC ^GATC
NlaIV 147 GGAACC GG[ACGT]^[ACGT]CC
NsiI 466 ATGCAT ATGCA^T
PleI 633 GACTC GAGTC(4/5)
PpuMI 682 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 428 CAGCTG CAG^CTG
RsaI 847 GTAC GT^AC
Sau3AI 122 GATC ^GATC
SfaNI 465 GATGC GCATC(5/9)
StyI 614 CCATGG C^C[AT][AT]GG
XhoII 121 GGATCT [GA]^GATC[CT]
XmnI 185 GAAACCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 541 CCGC CCGC(-3/-1)
433 GCGG
AlwNI 55 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
681 CAGGTCCTG
BsaJI 407 CCCAGG C^C[ACGT][ACGT]GG
614 CCATGG
BsgI 482 CTGCAC GTGCAG(16/14)
252 GTGCAG
BspHI 398 TCATGA T^CATGA
476 TCATGA
BssKI 408 CCAGG ^CC[ACGT]GG
437 CCTGG
BstNI 408 CCAGG CC^[AT]GG
437 CCTGG
EarI 380 CTCTTC CTCTTC(1/4)
635 CTCTTC
EcoRII 408 CCAGG ^CC[AT]GG
437 CCTGG
Ksp632I 380 CTCTTC CTCTTC(1/4)
635 CTCTTC
MvaI 408 CCAGG CC^[AT]GG
437 CCTGG
PflMI 306 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCATTTTATGG
PstI 61 CTGCAG CTGCA^G
687 CTGCAG
RcaI 398 TCATGA T^CATGA
476 TCATGA
ScrFI 408 CCAGG CC^[ACGT]GG
437 CCTGG
TfiI 801 GATTC G^A[AT]TC
852 GAATC
Tsp509I 551 AATT ^AATT
657 AATT
Van91I 306 CCACTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCATTTTATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI AvaII BbvI BsiYI BslI
BsrI Cfr13I DdeI Fnu4HI HaeIII
HinfI HphI ItaI MaeIII MboII
MnlI MseI MslI MwoI NlaIII
Sau96I SfcI Tru9I TseI Tsp45I
TspRI