Restriction map for
pFN21AB7123
(Circular) (Six-base) MAPSORT of: pFN21AB7123.seq Check: 9203 from: 1 to: 5638
pFN21AB7123 5638 bp
With 182 enzymes: SgfI *
January 16, 2009 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3448 278
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2819 2981 1937
Size: 882 162 4594
Fragments arranged by size:
4594 882 162
Acc65I G'GTAC_C
Cuts at: 2959 2959
Size: 5638
AclI AA'CG_TT
Cuts at: 1563 3765 4138 1563
Size: 2202 373 3063
Fragments arranged by size:
3063 2202 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2214 2599 3701 5085 5353 1632
Size: 582 385 1102 1384 268 1917
Fragments arranged by size:
1917 1384 1102 582 385 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5441
AflIII A'CryG_T
Cuts at: 1203 4537 5514 1203
Size: 3334 977 1327
Fragments arranged by size:
3334 1327 977
AgeI A'CCGG_T
Cuts at: 4469 4469
Size: 5638
AhdI GACnn_n'nnGTC
Cuts at: 4367 4367
Size: 5638
AleI CACnn'nnGTG
Cuts at: 1166 2504 1166
Size: 1338 4300
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5638
AlwNI CAG_nnn'CTG
Cuts at: 3381 4953 3381
Size: 1572 4066
ApaLI G'TGCA_C
Cuts at: 2507 3695 4851 2507
Size: 1188 1156 3294
Fragments arranged by size:
3294 1188 1156
ApoI r'AATT_y
Cuts at: 1493 2599 2947 3173 3325 5434 1493
Size: 1106 348 226 152 2109 1697
Fragments arranged by size:
2109 1697 1106 348 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4192 160
Size: 872 3160 1606
Fragments arranged by size:
3160 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
AvaI C'yCGr_G
Cuts at: 1870 1943 2963 1870
Size: 73 1020 4545
Fragments arranged by size:
4545 1020 73
BamHI G'GATC_C
Cuts at: 2119 2968 3335 2119
Size: 849 367 4422
Fragments arranged by size:
4422 849 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2411 2561 2959 4414 618
Size: 325 217 627 624 150 398 1455 1842
Fragments arranged by size:
1842 1455 627 624 398 325 217 150
BanII G_rGCy'C
Cuts at: 1391 2405 2583 2957 1391
Size: 1014 178 374 4072
Fragments arranged by size:
4072 1014 374 178
BbeI G_GCGC'C
Cuts at: 2415 2415
Size: 5638
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4667
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3829 3863 1903
Size: 34 1892 34 3678
Fragments arranged by size:
3678 1892 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3530 4740 3530
Size: 1210 4428
BclI T'GATC_A
Cuts at: 1548 2349 1548
Size: 801 4837
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2409 2519 4249
Size: 107 122 71 631 605 737 110 1730
Cuts at: 4249 136
Size: 1525
Fragments arranged by size:
1730 1525 737 631 605 122 110 107
71
BglII A'GATC_T
Cuts at: 5633 5633
Size: 5638
BlpI GC'TnA_GC
Cuts at: 3055 3055
Size: 5638
Bme1580I G_kGCm'C
Cuts at: 1790 2511 2551 3699 4855 1790
Size: 721 40 1148 1156 2573
Fragments arranged by size:
2573 1156 1148 721 40
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5638
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2561 3369 4327
Size: 416 53 83 399 24 1144 808 958
Cuts at: 4327 442
Size: 1753
Fragments arranged by size:
1753 1144 958 808 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5638
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4298 1239
Size: 3059 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3763 4628 4926 3763
Size: 865 298 4475
Fragments arranged by size:
4475 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2334 4301 882
Size: 617 835 1967 2219
Fragments arranged by size:
2219 1967 835 617
BsaAI yAC'GTr
Cuts at: 493 1204 3429 493
Size: 711 2225 2702
Fragments arranged by size:
2702 2225 711
BsaBI GATnn'nnATC
Cuts at: 5632 5632
Size: 5638
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2412 3445 3827 4462
Size: 53 83 186 936 879 1033 382 635
Cuts at: 4462 275
Size: 1451
Fragments arranged by size:
1451 1033 936 879 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4071 4469 4743 4890 5301 4071
Size: 398 274 147 411 4408
Fragments arranged by size:
4408 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2082 2112 2331 2361 2642
Size: 30 165 30 679 30 219 30 281
Cuts at: 2642 2672 2801 2831 1178
Size: 30 129 30 3985
Fragments arranged by size:
3985 679 281 219 165 129 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5638
BseYI C'CCAG_C
Cuts at: 2303 2404 2524 2825 4841 5337 2303
Size: 101 120 301 2016 496 2604
Fragments arranged by size:
2604 2016 496 301 120 101
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2060 2097 2301 1163
Size: 573 324 37 204 4500
Fragments arranged by size:
4500 573 324 204 37
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3088 3849 4877 1540
Size: 275 186 1087 761 1028 2301
Fragments arranged by size:
2301 1087 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2511 3699 3784 4855 2511
Size: 1188 85 1071 3294
Fragments arranged by size:
3294 1188 1071 85
BsmI GAATG_Cn'
Cuts at: 3149 3242 3149
Size: 93 5545
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2405 2511 2551 2583 2957 3699 3784
Size: 399 615 106 40 32 374 742 85
Cuts at: 3784 4855 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 615 399 374 106 85
40 32
BspEI T'CCGG_A
Cuts at: 5301 5301
Size: 5638
BspHI T'CATG_A
Cuts at: 3527 5257 3527
Size: 1730 3908
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2000 2993 844
Size: 1156 993 3489
Fragments arranged by size:
3489 1156 993
BsrBI CCG'CTC
Cuts at: 1394 1823 2173 2934 3525 1394
Size: 429 350 761 591 3507
Fragments arranged by size:
3507 761 591 429 350
BsrDI GCAATG_nn'
Cuts at: 65 1248 2236 4133 4307 65
Size: 1183 988 1897 174 1396
Fragments arranged by size:
1897 1396 1183 988 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4282 4469 1700
Size: 110 2472 187 2869
Fragments arranged by size:
2869 2472 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5638
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5638
BssSI C'ACGA_G
Cuts at: 3698 4710 3698
Size: 1012 4626
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2515 2729 794
Size: 1721 214 3703
Fragments arranged by size:
3703 1721 214
BstBI TT'CG_AA
Cuts at: 4442 4442
Size: 5638
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5638
BstXI CCAn_nnnn'nTGG
Cuts at: 2335 2476 2335
Size: 141 5497
BstYI r'GATC_y
Cuts at: 1972 2119 2968 3335 3720 3737 5189 5275 5287
Size: 147 849 367 385 17 1452 86 12
Cuts at: 5287 5633 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 849 385 367 346 147 86
17 12
BstZ17I GTA'TAC
Cuts at: 2820 2820
Size: 5638
Bsu36I CC'TnA_GG
Cuts at: 2154 2154
Size: 5638
BtgI C'CryG_G
Cuts at: 513 1065 2004 2124 2367 2682 513
Size: 552 939 120 243 315 3469
Fragments arranged by size:
3469 939 552 315 243 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4899
Fragments arranged by size:
4899 683 56
BtsI GCAGTG_nn'
Cuts at: 2499 2637 3154 3948 3968 2499
Size: 138 517 794 20 4169
Fragments arranged by size:
4169 794 517 138 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5638
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5603
DraI TTT'AAA
Cuts at: 2942 2942
Size: 5638
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4645 809
Size: 507 3329 1802
Fragments arranged by size:
3329 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2466 3085 3974
Size: 54 1425 50 275 18 636 619 889
Cuts at: 3974 5603 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 636 619 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3085 1537
Size: 275 1273 4090
Fragments arranged by size:
4090 1273 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2117 2201 3568 1353
Size: 764 84 1367 3423
Fragments arranged by size:
3423 1367 764 84
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2038 2413 4225 4599 4745 387
Size: 1064 138 449 375 1812 374 146 1280
Fragments arranged by size:
1812 1280 1064 449 375 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2214 2599 3701 4298 5085 5353 1239
Size: 393 582 385 1102 597 787 268 1524
Fragments arranged by size:
1524 1102 787 597 582 393 385 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2467 2467
Size: 5638
EcoO109I rG'GnC_Cy
Cuts at: 2012 2541 2591 2012
Size: 529 50 5059
Fragments arranged by size:
5059 529 50
EcoRI G'AATT_C
Cuts at: 2947 3325 2947
Size: 378 5260
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2479 2479
Size: 5638
FspI TGC'GCA
Cuts at: 2786 4144 2786
Size: 1358 4280
HaeII r_GCGC'y
Cuts at: 1678 2415 4785 1678
Size: 737 2370 2531
Fragments arranged by size:
2531 2370 737
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2082 2114 2908 2940 4279
Size: 32 135 32 510 32 794 32 1339
Cuts at: 4279 4311 4353 4385 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1339 794 510 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2982 3228 669
Size: 988 281 1044 246 3079
Fragments arranged by size:
3079 1044 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2759 748
Size: 2011 3627
HpaI GTT'AAC
Cuts at: 3228 3228
Size: 5638
KasI G'GCGC_C
Cuts at: 2411 2411
Size: 5638
KpnI G_GTAC'C
Cuts at: 2963 2963
Size: 5638
MfeI C'AATT_G
Cuts at: 3237 3237
Size: 5638
MluI A'CGCG_T
Cuts at: 5514 5514
Size: 5638
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4752 4936 1305
Size: 3447 184 2007
Fragments arranged by size:
3447 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2468 10
Size: 54 1425 343 636 3180
Fragments arranged by size:
3180 1425 636 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2504 3596 3955 4114 518
Size: 648 1338 1092 359 159 2042
Fragments arranged by size:
2042 1338 1092 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2829 3054 3731 4879 5124 1606
Size: 579 644 225 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 644 579 245 225
NarI GG'CG_CC
Cuts at: 2412 2412
Size: 5638
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4147
Fragments arranged by size:
4147 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5638
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5638
NotI GC'GGCC_GC
Cuts at: 3085 3085
Size: 5638
NspI r_CATG'y
Cuts at: 1132 1229 2996 4541 1132
Size: 97 1767 1545 2229
Fragments arranged by size:
2229 1767 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2575 1348
Size: 1227 4411
PciI A'CATG_T
Cuts at: 4537 4537
Size: 5638
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2368 1108
Size: 1260 4378
PfoI T'CCnGG_A
Cuts at: 2115 2115
Size: 5638
PmeI GTTT'AAAC
Cuts at: 2942 2942
Size: 5638
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3707 3739 5220 5252 3707
Size: 32 1481 32 4093
Fragments arranged by size:
4093 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2541 2012
Size: 529 5109
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5638
PsiI TTA'TAA
Cuts at: 3208 3208
Size: 5638
PstI C_TGCA'G
Cuts at: 830 2190 2725 2904 2990 830
Size: 1360 535 179 86 3478
Fragments arranged by size:
3478 1360 535 179 86
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5638
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 5638
RsrII CG'GwC_CG
Cuts at: 2025 2025
Size: 5638
SalI G'TCGA_C
Cuts at: 1936 2980 1936
Size: 1044 4594
SbfI CC_TGCA'GG
Cuts at: 2990 2990
Size: 5638
ScaI AGT'ACT
Cuts at: 2147 3886 5598 2147
Size: 1739 1712 2187
Fragments arranged by size:
2187 1739 1712
SfcI C'TryA_G
Cuts at: 826 1045 2186 2210 2721 2900 2986 4121 4802
Size: 219 1141 24 511 179 86 1135 681
Cuts at: 4802 4993 826
Size: 191 1471
Fragments arranged by size:
1471 1141 1135 681 511 219 191 179
86 24
SfoI GGC'GCC
Cuts at: 2413 2413
Size: 5638
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5638
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5638
SmaI CCC'GGG
Cuts at: 1872 2965 1872
Size: 1093 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3742 4643 4905 820
Size: 197 926 1799 901 262 1553
Fragments arranged by size:
1799 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3429 493
Size: 2936 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5638
SphI G_CATG'C
Cuts at: 1132 2996 1132
Size: 1864 3774
SspI AAT'ATT
Cuts at: 5 52 3562 5
Size: 47 3510 2081
Fragments arranged by size:
3510 2081 47
StuI AGG'CCT
Cuts at: 1517 2058 2082 1517
Size: 541 24 5073
Fragments arranged by size:
5073 541 24
StyI C'CwwG_G
Cuts at: 513 1065 2004 2463 3077 513
Size: 552 939 459 614 3074
Fragments arranged by size:
3074 939 614 552 459
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2549 2839 3665 3682 3835 4020 4465
Size: 1255 969 290 826 17 153 185 445
Cuts at: 4465 325
Size: 1498
Fragments arranged by size:
1498 1255 969 826 445 290 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2145 2568 3884
Size: 274 80 33 51 1443 168 423 1316
Cuts at: 3884 5596 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1316 423 274 168 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2331 2347 2363 2379 5598 5630 2331
Size: 16 16 16 3219 32 2339
Fragments arranged by size:
3219 2339 32 16 16 16
Tth111I GACn'n_nGTC
Cuts at: 1316 2437 1316
Size: 1121 4517
XbaI T'CTAG_A
Cuts at: 2974 2974
Size: 5638
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2572 2572
Size: 5638
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5638
XmaI C'CCGG_G
Cuts at: 1870 2963 1870
Size: 1093 4545
XmnI GAAnn'nnTTC
Cuts at: 3767 3767
Size: 5638
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3446 276
Size: 53 83 186 936 1912 2468
Fragments arranged by size:
2468 1912 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NarI NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII
SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoRV
FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI
PspOMI PspXI PsrI SacI SacII SanDI SapI SexAI
SfiI SrfI SwaI