Restriction map for
pFN21AB7122
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspLU11I BsrBI
BsrFI BsrI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HapII HhaI Hin1I HinP1I HindIII
HpaI HpaII KasI KpnI KspI
MaeI MamI MfeI MflI MluI
MroI MspI MunI MvnI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NlaIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SwaI
TaqI TfiI Tth111I TthHB8I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 241 GTAGAC GT^[AC][GT]AC
AcsI 25 GAATTC [GA]^AATT[CT]
AfaI 765 GTAC GT^AC
AlwI 326 GGATC GGATC(4/5)
AlwNI 151 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 25 GAATTC [GA]^AATT[CT]
Asp700I 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 258 GAGCAC G[AT]GC[AT]^C
AvaII 502 GGTCC G^G[AT]CC
BmyI 258 GAGCAC G[AGT]GC[ACT]^C
BpmI 55 CTGGAG CTGGAG(16/14)
BsaAI 361 TACGTG [CT]AC^GT[GA]
BsaI 40 GGTCTC GGTCTC(1/5)
BseRI 334 CTCCTC GAGGAG(10/8)
BsiHKAI 258 GAGCAC G[AT]GC[AT]^C
BsmAI 41 GTCTC GTCTC(1/5)
BsmI 864 GAATGC GAATGC(1/-1)
Bsp1286I 258 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 764 TGTACA T^GTACA
BspHI 398 TCATGA T^CATGA
BspMI 709 ACCTGC ACCTGC(4/8)
BsrGI 764 TGTACA T^GTACA
BstEII 161 GGTCACC G^GT[ACGT]ACC
BstPI 161 GGTCACC G^GT[ACGT]ACC
BstXI 104 CCACAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 406 CCACGG C^C[GA][CT]GG
EarI 475 CTCTTC CTCTTC(1/4)
EcoO65I 161 GGTCACC G^GT[ACGT]ACC
EcoRI 25 GAATTC G^AATTC
EcoT14I 230 CCAAGG C^C[AT][AT]GG
HgaI 640 GCGTC GACGC(5/10)
HincII 546 GTTGAC GT[CT]^[GA]AC
HindII 546 GTTGAC GT[CT]^[GA]AC
Ksp632I 475 CTCTTC CTCTTC(1/4)
MaeII 362 ACGT A^CGT
MspA1I 292 CAGCTG C[AC]G^C[GT]G
PflMI 570 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 711 CTGCAG CTGCA^G
PvuII 292 CAGCTG CAG^CTG
RcaI 398 TCATGA T^CATGA
RsaI 765 GTAC GT^AC
SfcI 711 CTGCAG C^T[GA][CT]AG
SspBI 764 TGTACA T^GTACA
SspI 69 AATATT AAT^ATT
StuI 772 AGGCCT AGG^CCT
StyI 230 CCAAGG C^C[AT][AT]GG
Van91I 570 CCACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 25 GAATTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 357 CCGC CCGC(-3/-1)
690 GCGG
BalI 365 TGGCCA TGG^CCA
617 TGGCCA
BsgI 382 CTGCAC GTGCAG(16/14)
558 CTGCAC
BsmFI 192 GTCCC GGGAC(10/14)
748 GTCCC
BsrDI 460 GCAATG GCAATG(2/0)
802 GCAATG
BssKI 54 CCTGG ^CC[ACGT]GG
576 CCTGG
BstNI 54 CCTGG CC^[AT]GG
576 CCTGG
Cac8I 456 GCACGC GC[ACGT]^[ACGT]GC
556 GCCTGC
Cfr13I 502 GGTCC G^G[ACGT]CC
579 GGGCC
EaeI 365 TGGCCA [CT]^GGCC[GA]
617 TGGCCA
EcoRII 54 CCTGG ^CC[AT]GG
576 CCTGG
MluNI 365 TGGCCA TGG^CCA
617 TGGCCA
MscI 365 TGGCCA TGG^CCA
617 TGGCCA
MseI 634 TTAA T^TAA
838 TTAA
MvaI 54 CCTGG CC^[AT]GG
576 CCTGG
Sau96I 502 GGTCC G^G[ACGT]CC
579 GGGCC
ScrFI 54 CCTGG CC^[ACGT]GG
576 CCTGG
Tru9I 634 TTAA T^TAA
838 TTAA
Tsp509I 26 AATT ^AATT
636 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BstF5I DdeI DpnII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeIII
MboI MboII MnlI MslI NdeII
NlaIII PleI Sau3AI TseI Tsp45I
TspRI