Restriction map for
pFN21AB7116
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstUI BstXI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KpnI KspI MaeI MaeII
MaeIII MamI MfeI MluI MroI
MseI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tsp45I XbaI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcsI 517 AAATTC [GA]^AATT[CT]
Alw44I 248 GTGCAC G^TGCAC
AlwI 285 GATCC GGATC(4/5)
ApaLI 248 GTGCAC G^TGCAC
ApoI 517 AAATTC [GA]^AATT[CT]
AspI 140 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 494 CTCGAG C^[CT]CG[GA]G
AvaII 579 GGACC G^G[AT]CC
BalI 2 TGGCCA TGG^CCA
BbsI 468 GAAGAC GAAGAC(2/6)
BcnI 435 CCGGG CC^[GC]GG
BpuAI 468 GAAGAC GAAGAC(2/6)
BsgI 780 GTGCAG GTGCAG(16/14)
BsmAI 454 GAGAC GTCTC(1/5)
BsmBI 454 GAGACG CGTCTC(1/5)
BsmFI 129 GGGAC GGGAC(10/14)
BsoBI 494 CTCGAG C^[CT]CG[GA]G
BstYI 284 AGATCC [GA]^GATC[CT]
CfoI 176 GCGC GCG^C
DsaI 506 CCGTGG C^C[GA][CT]GG
EcoNI 372 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HhaI 176 GCGC GCG^C
HinP1I 176 GCGC G^CGC
HincII 64 GTTGAC GT[CT]^[GA]AC
HindII 64 GTTGAC GT[CT]^[GA]AC
MflI 284 AGATCC [GA]^GATC[CT]
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MslI 32 CACCTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 435 CCGGG CC^[GC]GG
PaeR7I 494 CTCGAG C^TCGAG
PflFI 140 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 159 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 144 GAGTC GAGTC(4/5)
SacI 491 GAGCTC GAGCT^C
SapI 545 GCTCTTC GCTCTTC(1/4)
SfaNI 809 GCATC GCATC(5/9)
SmlI 494 CTCGAG C^T[CT][GA]AG
TaqI 495 TCGA T^CGA
Tsp509I 518 AATT ^AATT
Tth111I 140 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 495 TCGA T^CGA
Van91I 159 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 668 CCATTGTTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 494 CTCGAG C^TCGAG
XhoII 284 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AciI 122 CCGC CCGC(-3/-1)
196 CCGC
AfaI 307 GTAC GT^AC
634 GTAC
AspHI 248 GTGCAC G[AT]GC[AT]^C
491 GAGCTC
BanI 460 GGCACC G^G[CT][GA]CC
735 GGTGCC
BciVI 339 GTATCC GTATCC(6/5)
431 GTATCC
BglI 400 GCCAAAAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
738 GCCCAGGAGGC
BseRI 376 GAGGAG GAGGAG(10/8)
379 GAGGAG
BsiHKAI 248 GTGCAC G[AT]GC[AT]^C
491 GAGCTC
Cac8I 409 GCCAGC GC[ACGT]^[ACGT]GC
600 GCTTGC
DpnII 285 GATC ^GATC
611 GATC
EarI 324 CTCTTC CTCTTC(1/4)
546 CTCTTC
EcoT14I 125 CCAAGG C^C[AT][AT]GG
589 CCAAGG
HapII 435 CCGG C^CGG
645 CCGG
HpaII 435 CCGG C^CGG
645 CCGG
HphI 613 TCACC GGTGA(8/7)
761 TCACC
Ksp632I 324 CTCTTC CTCTTC(1/4)
546 CTCTTC
MboI 285 GATC ^GATC
611 GATC
MspI 435 CCGG C^CGG
645 CCGG
NdeII 285 GATC ^GATC
611 GATC
NlaIII 105 CATG CATG^
465 CATG
RsaI 307 GTAC GT^AC
634 GTAC
Sau3AI 285 GATC ^GATC
611 GATC
StyI 125 CCAAGG C^C[AT][AT]GG
589 CCAAGG
TfiI 484 GATTC G^A[AT]TC
785 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AluI BanII BbvI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI EaeI
EcoRII Fnu4HI HaeIII HinfI ItaI
MboII MnlI MvaI MwoI NlaIV
PstI Sau96I ScrFI SfcI TseI
TspRI