Restriction map for
pFN21AB7116
(Linear) (Six-base) MAPSORT of: KIBB7116.seq Check: 5453 from: 1 to: 840
KIBB7116.orf
With 182 enzymes: SgfI *
March 3, 2008 10:58 ..
AleI CACnn'nnGTG
Cuts at: 0 36 840
Size: 36 804
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 685 717 840
Size: 685 32 123
Fragments arranged by size:
685 123 32
ApaLI G'TGCA_C
Cuts at: 0 248 840
Size: 248 592
ApoI r'AATT_y
Cuts at: 0 517 840
Size: 517 323
AvaI C'yCGr_G
Cuts at: 0 494 840
Size: 494 346
BanI G'GyrC_C
Cuts at: 0 460 735 840
Size: 460 275 105
Fragments arranged by size:
460 275 105
BanII G_rGCy'C
Cuts at: 0 441 495 547 840
Size: 441 54 52 293
Fragments arranged by size:
441 293 54 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 475 840
Size: 475 365
BciVI GTATCCnnnnn_n'
Cuts at: 0 350 442 840
Size: 350 92 398
Fragments arranged by size:
398 350 92
BglI GCCn_nnn'nGGC
Cuts at: 0 406 744 840
Size: 406 338 96
Fragments arranged by size:
406 338 96
Bme1580I G_kGCm'C
Cuts at: 0 252 463 740 840
Size: 252 211 277 100
Fragments arranged by size:
277 252 211 100
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 840
Size: 323 517
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 394 586 840
Size: 185 209 192 254
Fragments arranged by size:
254 209 192 185
Bpu10I CC'TnA_GC
Cuts at: 0 290 840
Size: 290 550
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 685 715 840
Size: 685 30 125
Fragments arranged by size:
685 125 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 394 840
Size: 391 3 446
Fragments arranged by size:
446 391 3
BseYI C'CCAG_C
Cuts at: 0 804 840
Size: 804 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 801 840
Size: 801 39
BsiHKAI G_wGCw'C
Cuts at: 0 252 495 840
Size: 252 243 345
Fragments arranged by size:
345 252 243
BsmBI CGTCTCn'nnnn_
Cuts at: 0 448 840
Size: 448 392
Bsp1286I G_dGCh'C
Cuts at: 0 252 441 463 495 547 740 840
Size: 252 189 22 32 52 193 100
Fragments arranged by size:
252 193 189 100 52 32 22
BstYI r'GATC_y
Cuts at: 0 284 840
Size: 284 556
BtgI C'CryG_G
Cuts at: 0 506 840
Size: 506 334
BtsI GCAGTG_nn'
Cuts at: 0 415 711 840
Size: 415 296 129
Fragments arranged by size:
415 296 129
EaeI y'GGCC_r
Cuts at: 0 2 646 680 840
Size: 2 644 34 160
Fragments arranged by size:
644 160 34 2
EarI CTCTTCn'nnn_
Cuts at: 0 330 552 840
Size: 330 222 288
Fragments arranged by size:
330 288 222
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 111 185 840
Size: 111 74 655
Fragments arranged by size:
655 111 74
EcoICRI GAG'CTC
Cuts at: 0 493 840
Size: 493 347
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 394 586 840
Size: 185 209 192 254
Fragments arranged by size:
254 209 192 185
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 840
Size: 376 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 559 591 840
Size: 559 32 249
Fragments arranged by size:
559 249 32
HincII GTy'rAC
Cuts at: 0 66 840
Size: 66 774
MscI TGG'CCA
Cuts at: 0 4 840
Size: 4 836
MslI CAynn'nnrTG
Cuts at: 0 36 840
Size: 36 804
PasI CC'CwG_GG
Cuts at: 0 159 840
Size: 159 681
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 840
Size: 165 675
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 840
Size: 685 32 123
Fragments arranged by size:
685 123 32
PspXI vC'TCGA_Gb
Cuts at: 0 494 840
Size: 494 346
PstI C_TGCA'G
Cuts at: 0 152 299 718 840
Size: 152 147 419 122
Fragments arranged by size:
419 152 147 122
SacI G_AGCT'C
Cuts at: 0 495 840
Size: 495 345
SapI GCTCTTCn'nnn_
Cuts at: 0 552 840
Size: 552 288
SfcI C'TryA_G
Cuts at: 0 148 295 714 840
Size: 148 147 419 126
Fragments arranged by size:
419 148 147 126
SmlI C'TyrA_G
Cuts at: 0 494 840
Size: 494 346
StyI C'CwwG_G
Cuts at: 0 125 589 840
Size: 125 464 251
Fragments arranged by size:
464 251 125
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 267 840
Size: 156 111 573
Fragments arranged by size:
573 156 111
Tth111I GACn'n_nGTC
Cuts at: 0 143 840
Size: 143 697
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 675 840
Size: 675 165
XhoI C'TCGA_G
Cuts at: 0 494 840
Size: 494 346
Enzymes that do cut:
AleI AloI ApaLI ApoI AvaI BanI BanII BbsI
BciVI BglI Bme1580I BmrI BpmI Bpu10I BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BstYI BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI Hin4I HincII
MscI MslI PasI PflMI PpiI PspXI PstI SacI
SapI SfcI SmlI StyI TaqII Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BclI
BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
XbaI XmaI XmnI ZraI