(Circular) (Six-base) MAPSORT of: pFN21AB7115.seq Check: 1457 from: 1 to: 5542 pFN21AB7115 5542 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3352 278 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 1937 Size: 948 4594 Acc65I G'GTAC_C Cuts at: 2863 2863 Size: 5542 AclI AA'CG_TT Cuts at: 1563 3669 4042 1563 Size: 2106 373 3063 Fragments arranged by size: 3063 2106 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3605 4989 5257 1632 Size: 1973 1384 268 1917 Fragments arranged by size: 1973 1917 1384 268 AfeI AGC'GCT Cuts at: 2573 2573 Size: 5542 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5345 AflIII A'CryG_T Cuts at: 1203 4441 5418 1203 Size: 3238 977 1327 Fragments arranged by size: 3238 1327 977 AgeI A'CCGG_T Cuts at: 4373 4373 Size: 5542 AhdI GACnn_n'nnGTC Cuts at: 4271 4271 Size: 5542 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5542 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2222 1184 Size: 1038 4504 AlwNI CAG_nnn'CTG Cuts at: 2089 2622 3285 4857 2089 Size: 533 663 1572 2774 Fragments arranged by size: 2774 1572 663 533 ApaLI G'TGCA_C Cuts at: 3599 4755 3599 Size: 1156 4386 ApoI r'AATT_y Cuts at: 1493 2851 3077 3229 5338 1493 Size: 1358 226 152 2109 1697 Fragments arranged by size: 2109 1697 1358 226 152 AseI AT'TA_AT Cuts at: 160 1032 4096 160 Size: 872 3064 1606 Fragments arranged by size: 3064 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 AvaI C'yCGr_G Cuts at: 1870 1943 2077 2812 2867 1870 Size: 73 134 735 55 4545 Fragments arranged by size: 4545 735 134 73 55 BamHI G'GATC_C Cuts at: 2451 2872 3239 2451 Size: 421 367 4754 Fragments arranged by size: 4754 421 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2443 2517 2863 4318 618 Size: 325 217 627 656 74 346 1455 1842 Fragments arranged by size: 1842 1455 656 627 346 325 217 74 BanII G_rGCy'C Cuts at: 1391 2861 1391 Size: 1470 4072 BbeI G_GCGC'C Cuts at: 2521 2521 Size: 5542 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3733 3767 1903 Size: 34 1796 34 3678 Fragments arranged by size: 3678 1796 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3434 4644 3434 Size: 1210 4332 BclI T'GATC_A Cuts at: 1548 2307 1548 Size: 759 4783 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2705 4153 136 Size: 107 122 71 631 605 1033 1448 1525 Fragments arranged by size: 1525 1448 1033 631 605 122 107 71 BglII A'GATC_T Cuts at: 5537 5537 Size: 5542 BlpI GC'TnA_GC Cuts at: 2959 2959 Size: 5542 Bme1580I G_kGCm'C Cuts at: 1790 3603 4759 1790 Size: 1813 1156 2573 Fragments arranged by size: 2573 1813 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5542 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3273 4231 442 Size: 416 53 83 399 24 1856 958 1753 Fragments arranged by size: 1856 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5542 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2285 4202 1239 Size: 1031 15 1917 2579 Fragments arranged by size: 2579 1917 1031 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3667 4532 4830 3667 Size: 865 298 4379 Fragments arranged by size: 4379 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4205 882 Size: 617 2706 2219 Fragments arranged by size: 2706 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3333 493 Size: 711 2129 2702 Fragments arranged by size: 2702 2129 711 BsaBI GATnn'nnATC Cuts at: 5536 5536 Size: 5542 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2518 3349 3731 4366 Size: 53 83 186 936 985 831 382 635 Cuts at: 4366 275 Size: 1451 Fragments arranged by size: 1451 985 936 831 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3975 4373 4647 4794 5205 3975 Size: 398 274 147 411 4312 Fragments arranged by size: 4312 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2732 2762 1178 Size: 30 165 30 1329 30 3958 Fragments arranged by size: 3958 1329 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2399 2672 2681 1186 Size: 1081 132 273 9 4047 Fragments arranged by size: 4047 1081 273 132 9 BseYI C'CCAG_C Cuts at: 2113 2426 2643 2789 4745 5241 2113 Size: 313 217 146 1956 496 2414 Fragments arranged by size: 2414 1956 496 313 217 146 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2470 1163 Size: 573 734 4235 Fragments arranged by size: 4235 734 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2343 2992 3753 4781 1540 Size: 275 186 342 649 761 1028 2301 Fragments arranged by size: 2301 1028 761 649 342 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2439 2564 3603 3688 4759 2211 Size: 228 125 1039 85 1071 2994 Fragments arranged by size: 2994 1071 1039 228 125 85 BsmI GAATG_Cn' Cuts at: 3053 3146 3053 Size: 93 5449 BsmBI CGTCTCn'nnnn_ Cuts at: 2273 2273 Size: 5542 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2211 2439 2564 2861 3603 3688 4759 Size: 399 421 228 125 297 742 85 1071 Cuts at: 4759 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 421 399 297 228 125 85 BspEI T'CCGG_A Cuts at: 5205 5205 Size: 5542 BspHI T'CATG_A Cuts at: 3431 5161 3431 Size: 1730 3812 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2897 844 Size: 2053 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3429 1394 Size: 429 1606 3507 Fragments arranged by size: 3507 1606 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4037 4211 65 Size: 1183 2789 174 1396 Fragments arranged by size: 2789 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4186 4373 1700 Size: 110 2376 187 2869 Fragments arranged by size: 2869 2376 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5542 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5542 BssSI C'ACGA_G Cuts at: 2512 3602 4614 2512 Size: 1090 1012 3440 Fragments arranged by size: 3440 1090 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2484 2622 794 Size: 1690 138 3714 Fragments arranged by size: 3714 1690 138 BstBI TT'CG_AA Cuts at: 4346 4346 Size: 5542 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5542 BstXI CCAn_nnnn'nTGG Cuts at: 2797 2797 Size: 5542 BstYI r'GATC_y Cuts at: 1972 2451 2872 3239 3624 3641 5093 5179 5191 Size: 479 421 367 385 17 1452 86 12 Cuts at: 5191 5537 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 479 421 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2412 2466 513 Size: 552 939 408 54 3589 Fragments arranged by size: 3589 939 552 408 54 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2032 487 Size: 683 56 806 3997 Fragments arranged by size: 3997 806 683 56 BtsI GCAGTG_nn' Cuts at: 2061 2524 3058 3852 3872 2061 Size: 463 534 794 20 3731 Fragments arranged by size: 3731 794 534 463 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5542 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5507 DraI TTT'AAA Cuts at: 2846 2846 Size: 5542 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4549 809 Size: 507 3233 1802 Fragments arranged by size: 3233 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2019 2316 2340 Size: 54 1425 50 275 18 189 297 24 Cuts at: 2340 2637 2989 3878 5507 8 Size: 297 352 889 1629 43 Fragments arranged by size: 1629 1425 889 352 297 297 275 189 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2340 2989 1537 Size: 275 528 649 4090 Fragments arranged by size: 4090 649 528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3472 1353 Size: 2119 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2678 4129 4503 4649 387 Size: 1064 138 1089 1451 374 146 1280 Fragments arranged by size: 1451 1280 1089 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2285 3605 4202 4989 5257 1239 Size: 393 638 15 1320 597 787 268 1524 Fragments arranged by size: 1524 1320 787 638 597 393 268 15 EcoNI CCTnn'n_nnAGG Cuts at: 2252 2252 Size: 5542 EcoO109I rG'GnC_Cy Cuts at: 2073 2682 2073 Size: 609 4933 EcoRI G'AATT_C Cuts at: 2851 3229 2851 Size: 378 5164 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2344 2557 2344 Size: 213 5329 FspI TGC'GCA Cuts at: 4048 4048 Size: 5542 HaeII r_GCGC'y Cuts at: 1678 2096 2362 2521 2575 4689 1678 Size: 418 266 159 54 2114 2531 Fragments arranged by size: 2531 2114 418 266 159 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2732 2764 4183 4215 4257 Size: 32 135 32 1160 32 1419 32 42 Cuts at: 4257 4289 1373 Size: 32 2626 Fragments arranged by size: 2626 1419 1160 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2886 3132 669 Size: 988 281 948 246 3079 Fragments arranged by size: 3079 988 948 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5542 HpaI GTT'AAC Cuts at: 3132 3132 Size: 5542 KasI G'GCGC_C Cuts at: 2517 2517 Size: 5542 KpnI G_GTAC'C Cuts at: 2867 2867 Size: 5542 MfeI C'AATT_G Cuts at: 3141 3141 Size: 5542 MluI A'CGCG_T Cuts at: 5418 5418 Size: 5542 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2775 4656 4840 1305 Size: 1470 1881 184 2007 Fragments arranged by size: 2007 1881 1470 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2021 10 Size: 54 1425 343 189 3531 Fragments arranged by size: 3531 1425 343 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2477 3500 3859 4018 518 Size: 648 1023 288 1023 359 159 2042 Fragments arranged by size: 2042 1023 1023 648 359 288 159 MspA1I CmG'CkG Cuts at: 1606 2089 2301 2650 2958 3635 4783 5028 1606 Size: 483 212 349 308 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 483 349 308 245 212 NarI GG'CG_CC Cuts at: 2518 2518 Size: 5542 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4051 Fragments arranged by size: 4051 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5542 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5542 NotI GC'GGCC_GC Cuts at: 2989 2989 Size: 5542 NspI r_CATG'y Cuts at: 1132 1229 2482 2900 4445 1132 Size: 97 1253 418 1545 2229 Fragments arranged by size: 2229 1545 1253 418 97 PasI CC'CwG_GG Cuts at: 1348 2687 1348 Size: 1339 4203 PciI A'CATG_T Cuts at: 4441 4441 Size: 5542 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5542 PmeI GTTT'AAAC Cuts at: 2846 2846 Size: 5542 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3611 3643 5124 5156 3611 Size: 32 1481 32 3997 Fragments arranged by size: 3997 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2682 2682 Size: 5542 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5542 PsiI TTA'TAA Cuts at: 3112 3112 Size: 5542 PstI C_TGCA'G Cuts at: 830 2289 2618 2894 830 Size: 1459 329 276 3478 Fragments arranged by size: 3478 1459 329 276 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5542 SalI G'TCGA_C Cuts at: 1936 2884 1936 Size: 948 4594 SanDI GG'GwC_CC Cuts at: 2682 2682 Size: 5542 SbfI CC_TGCA'GG Cuts at: 2894 2894 Size: 5542 ScaI AGT'ACT Cuts at: 3790 5502 3790 Size: 1712 3830 SfcI C'TryA_G Cuts at: 826 1045 2285 2614 2890 4025 4706 4897 826 Size: 219 1240 329 276 1135 681 191 1471 Fragments arranged by size: 1471 1240 1135 681 329 276 219 191 SfoI GGC'GCC Cuts at: 2519 2519 Size: 5542 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5542 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5542 SmaI CCC'GGG Cuts at: 1872 2869 1872 Size: 997 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3646 4547 4809 820 Size: 197 926 1703 901 262 1553 Fragments arranged by size: 1703 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3333 493 Size: 2840 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5542 SphI G_CATG'C Cuts at: 1132 2482 2900 1132 Size: 1350 418 3774 Fragments arranged by size: 3774 1350 418 SspI AAT'ATT Cuts at: 5 52 3466 5 Size: 47 3414 2081 Fragments arranged by size: 3414 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5542 StyI C'CwwG_G Cuts at: 513 1065 2004 2981 513 Size: 552 939 977 3074 Fragments arranged by size: 3074 977 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3569 3586 3739 3924 4369 325 Size: 1255 1989 17 153 185 445 1498 Fragments arranged by size: 1989 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3788 5500 96 Size: 274 80 33 51 1443 1811 1712 138 Fragments arranged by size: 1811 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2436 2468 5502 5534 2436 Size: 32 3034 32 2444 Fragments arranged by size: 3034 2444 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5542 XbaI T'CTAG_A Cuts at: 2878 2878 Size: 5542 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5542 XmaI C'CCGG_G Cuts at: 1870 2867 1870 Size: 997 4545 XmnI GAAnn'nnTTC Cuts at: 3671 3671 Size: 5542 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3350 276 Size: 53 83 186 936 1816 2468 Fragments arranged by size: 2468 1816 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SrfI SwaI XcmI