Restriction map for
pFN21AB7111
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AlwNI AocI
ApaLI ApoI AscI AseI AsnI
AspEI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BsrI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DdeI DraI DraIII DrdI DsaI
EagI Eam1105I EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI Hin1I HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MroI MunI MvnI
MwoI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tsp509I TthHB8I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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Acc65I 479 GGTACC G^GTACC
Aor51HI 261 AGCGCT AGC^GCT
ApaI 230 GGGCCC GGGCC^C
Asp700I 336 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 479 GGTACC G^GTACC
AspHI 671 GAGCAC G[AT]GC[AT]^C
AspI 148 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 272 TGGCCA TGG^CCA
BanI 479 GGTACC G^G[CT][GA]CC
BanII 230 GGGCCC G[GA]GC[CT]^C
BbsI 561 GAAGAC GAAGAC(2/6)
BcgI 369 GCAGAAGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 548 CTAG C^TAG
BpmI 235 CTCCAG CTGGAG(16/14)
BpuAI 561 GAAGAC GAAGAC(2/6)
BseRI 323 GAGGAG GAGGAG(10/8)
BsgI 16 CTGCAC GTGCAG(16/14)
BsiHKAI 671 GAGCAC G[AT]GC[AT]^C
BsmAI 250 GTCTC GTCTC(1/5)
BsmBI 249 CGTCTC CGTCTC(1/5)
BspHI 98 TCATGA T^CATGA
BssSI 106 CTCGTG CACGAG(-5/-1)
BstXI 275 CCATGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 605 GCATGC GC[ACGT]^[ACGT]GC
CfoI 262 GCGC GCG^C
DraII 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 272 TGGCCA [CT]^GGCC[GA]
EarI 330 GAAGAG CTCTTC(1/4)
Eco47III 261 AGCGCT AGC^GCT
EcoNI 287 CCTACTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 230 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 277 ATGCAT ATGCA^T
HaeII 261 AGCGCT [GA]GCGC^[CT]
HhaI 262 GCGC GCG^C
HinP1I 262 GCGC G^CGC
KpnI 479 GGTACC GGTAC^C
Ksp632I 330 GAAGAG CTCTTC(1/4)
MaeI 548 CTAG C^TAG
MaeIII 134 GTGAC ^GT[ACGT]AC
MluNI 272 TGGCCA TGG^CCA
MscI 272 TGGCCA TGG^CCA
MspA1I 238 CAGCTG C[AC]G^C[GT]G
NsiI 277 ATGCAT ATGCA^T
PflFI 148 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 95 GAGTC GAGTC(4/5)
PvuII 238 CAGCTG CAG^CTG
RcaI 98 TCATGA T^CATGA
SfcI 291 CTACAG C^T[GA][CT]AG
SphI 605 GCATGC GCATG^C
TfiI 428 GAATC G^A[AT]TC
Tsp45I 134 GTGAC ^GT[GC]AC
TspRI 164 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 148 GACTTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 336 GAATGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AfaI 405 GTAC GT^AC
480 GTAC
AluI 239 AGCT AG^CT
737 AGCT
AlwI 102 GATCC GGATC(4/5)
71 GGATC
BcnI 172 CCGGG CC^[GC]GG
207 CCCGG
BmyI 230 GGGCCC G[AGT]GC[ACT]^C
671 GAGCAC
BsmFI 10 GGGAC GGGAC(10/14)
213 GTCCC
Bsp1286I 230 GGGCCC G[AGT]GC[ACT]^C
671 GAGCAC
BspMI 485 ACCTGC ACCTGC(4/8)
635 ACCTGC
BstF5I 219 CATCC GGATG(2/0)
175 GGATG
BstNI 120 CCAGG CC^[AT]GG
491 CCTGG
Cfr13I 230 GGGCC G^G[ACGT]CC
231 GGCCC
EcoRII 120 CCAGG ^CC[AT]GG
491 CCTGG
FokI 219 CATCC GGATG(9/13)
175 GGATG
HincII 37 GTCAAC GT[CT]^[GA]AC
532 GTTGAC
HindII 37 GTCAAC GT[CT]^[GA]AC
532 GTTGAC
HinfI 95 GAGTC G^A[ACGT]TC
428 GAATC
HphI 133 GGTGA GGTGA(8/7)
690 TCACC
MaeII 403 ACGT A^CGT
617 ACGT
MseI 362 TTAA T^TAA
614 TTAA
MslI 276 CATGCATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
576 CATTTTTATG
MvaI 120 CCAGG CC^[AT]GG
491 CCTGG
NciI 172 CCGGG CC^[GC]GG
207 CCCGG
NlaIV 230 GGGCCC GG[ACGT]^[ACGT]CC
479 GGTACC
NspI 279 GCATGT [GA]CATG^[CT]
605 GCATGC
RsaI 405 GTAC GT^AC
480 GTAC
Sau96I 230 GGGCC G^G[ACGT]CC
231 GGCCC
Tru9I 362 TTAA T^TAA
614 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
BbvI BsaJI BsiYI BslI BssKI
DpnII Fnu4HI HaeIII HapII HgaI
HpaII ItaI MboI MboII MnlI
MspI NdeII NlaIII Sau3AI ScrFI
SfaNI TseI