(Circular) (Six-base) MAPSORT of: pFN21AB7103.seq Check: 724 from: 1 to: 5362 pFN21AB7103 5362 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2059 2059 Size: 5362 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3172 278 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2705 1937 Size: 768 4594 Acc65I G'GTAC_C Cuts at: 2683 2683 Size: 5362 AclI AA'CG_TT Cuts at: 1563 3489 3862 1563 Size: 1926 373 3063 Fragments arranged by size: 3063 1926 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2578 3425 4809 5077 1632 Size: 946 847 1384 268 1917 Fragments arranged by size: 1917 1384 946 847 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5165 AflIII A'CryG_T Cuts at: 1203 4261 5238 1203 Size: 3058 977 1327 Fragments arranged by size: 3058 1327 977 AgeI A'CCGG_T Cuts at: 4193 4193 Size: 5362 AhdI GACnn_n'nnGTC Cuts at: 4091 4091 Size: 5362 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5362 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5362 AlwNI CAG_nnn'CTG Cuts at: 3105 4677 3105 Size: 1572 3790 ApaLI G'TGCA_C Cuts at: 3419 4575 3419 Size: 1156 4206 ApoI r'AATT_y Cuts at: 1493 2671 2897 3049 5158 1493 Size: 1178 226 152 2109 1697 Fragments arranged by size: 2109 1697 1178 226 152 AseI AT'TA_AT Cuts at: 160 1032 3916 160 Size: 872 2884 1606 Fragments arranged by size: 2884 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 AvaI C'yCGr_G Cuts at: 1870 1943 2687 1870 Size: 73 744 4545 Fragments arranged by size: 4545 744 73 BamHI G'GATC_C Cuts at: 2692 3059 2692 Size: 367 4995 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2683 4138 618 Size: 325 217 627 896 1455 1842 Fragments arranged by size: 1842 1455 896 627 325 217 BanII G_rGCy'C Cuts at: 1391 2270 2480 2681 1391 Size: 879 210 201 4072 Fragments arranged by size: 4072 879 210 201 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4391 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3553 3587 1903 Size: 34 1616 34 3678 Fragments arranged by size: 3678 1616 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3254 4464 3254 Size: 1210 4152 BclI T'GATC_A Cuts at: 1548 1548 Size: 5362 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2405 2577 3973 Size: 107 122 71 631 605 733 172 1396 Cuts at: 3973 136 Size: 1525 Fragments arranged by size: 1525 1396 733 631 605 172 122 107 71 BglII A'GATC_T Cuts at: 5357 5357 Size: 5362 BlpI GC'TnA_GC Cuts at: 2779 2779 Size: 5362 Bme1580I G_kGCm'C Cuts at: 1790 2219 3423 4579 1790 Size: 429 1204 1156 2573 Fragments arranged by size: 2573 1204 1156 429 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3093 4051 442 Size: 416 53 83 399 24 1676 958 1753 Fragments arranged by size: 1753 1676 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2210 1056 Size: 1154 4208 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4022 1239 Size: 2783 2579 Bpu10I CC'TnA_GC Cuts at: 2191 2191 Size: 5362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2007 3487 4352 4650 2007 Size: 1480 865 298 2719 Fragments arranged by size: 2719 1480 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2627 4025 882 Size: 617 1128 1398 2219 Fragments arranged by size: 2219 1398 1128 617 BsaAI yAC'GTr Cuts at: 493 1204 3153 493 Size: 711 1949 2702 Fragments arranged by size: 2702 1949 711 BsaBI GATnn'nnATC Cuts at: 5356 5356 Size: 5362 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2343 2626 3169 3551 Size: 53 83 186 936 810 283 543 382 Cuts at: 3551 4186 275 Size: 635 1451 Fragments arranged by size: 1451 936 810 635 543 382 283 186 83 53 BsaWI w'CCGG_w Cuts at: 3795 4193 4467 4614 5025 3795 Size: 398 274 147 411 4132 Fragments arranged by size: 4132 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2519 2549 1178 Size: 30 165 30 663 30 423 30 3991 Fragments arranged by size: 3991 663 423 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5362 BseYI C'CCAG_C Cuts at: 2088 2491 4565 5061 2088 Size: 403 2074 496 2389 Fragments arranged by size: 2389 2074 496 403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 1163 Size: 573 561 4228 Fragments arranged by size: 4228 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2432 2812 3573 4601 1540 Size: 275 186 431 380 761 1028 2301 Fragments arranged by size: 2301 1028 761 431 380 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2270 3423 3508 4579 2226 Size: 44 1153 85 1071 3009 Fragments arranged by size: 3009 1153 1071 85 44 BsmI GAATG_Cn' Cuts at: 2873 2966 2873 Size: 93 5269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2226 2270 2480 2681 3423 3508 Size: 399 429 7 44 210 201 742 85 Cuts at: 3508 4579 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 429 399 210 201 85 44 7 BspEI T'CCGG_A Cuts at: 5025 5025 Size: 5362 BspHI T'CATG_A Cuts at: 3251 4981 3251 Size: 1730 3632 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2717 844 Size: 1215 658 3489 Fragments arranged by size: 3489 1215 658 BsrBI CCG'CTC Cuts at: 1394 1823 2190 2523 3249 1394 Size: 429 367 333 726 3507 Fragments arranged by size: 3507 726 429 367 333 BsrDI GCAATG_nn' Cuts at: 65 1248 2457 2646 3857 4031 65 Size: 1183 1209 189 1211 174 1396 Fragments arranged by size: 1396 1211 1209 1183 189 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2384 2622 4006 4193 1700 Size: 110 574 238 1384 187 2869 Fragments arranged by size: 2869 1384 574 238 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5362 BssSI C'ACGA_G Cuts at: 2135 2348 2369 2630 3422 4434 2135 Size: 213 21 261 792 1012 3063 Fragments arranged by size: 3063 1012 792 261 213 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5362 BstBI TT'CG_AA Cuts at: 4166 4166 Size: 5362 BstEII G'GTnAC_C Cuts at: 1184 2380 1184 Size: 1196 4166 BstXI CCAn_nnnn'nTGG Cuts at: 2545 2545 Size: 5362 BstYI r'GATC_y Cuts at: 1972 2692 3059 3444 3461 4913 4999 5011 5357 Size: 720 367 385 17 1452 86 12 346 Cuts at: 5357 1972 Size: 1977 Fragments arranged by size: 1977 1452 720 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2096 2127 2572 2581 513 Size: 552 1031 31 445 9 3294 Fragments arranged by size: 3294 1031 552 445 31 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4623 Fragments arranged by size: 4623 683 56 BtsI GCAGTG_nn' Cuts at: 2165 2332 2527 2878 3672 3692 2165 Size: 167 195 351 794 20 3835 Fragments arranged by size: 3835 794 351 195 167 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5362 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5327 DraI TTT'AAA Cuts at: 2666 2666 Size: 5362 DraIII CAC_nnn'GTG Cuts at: 2311 2311 Size: 5362 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4369 809 Size: 507 3053 1802 Fragments arranged by size: 3053 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2397 2406 Size: 54 1425 50 275 18 294 273 9 Cuts at: 2406 2429 2436 2547 2569 2578 2620 2809 3698 Size: 23 7 111 22 9 42 189 889 Cuts at: 3698 5327 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 294 275 273 189 111 54 50 43 42 23 22 18 9 9 7 EagI C'GGCC_G Cuts at: 1537 1812 2429 2809 1537 Size: 275 617 380 4090 Fragments arranged by size: 4090 617 380 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3292 1353 Size: 1939 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 3949 4323 4469 387 Size: 1064 138 696 1664 374 146 1280 Fragments arranged by size: 1664 1280 1064 696 374 146 138 EcoICRI GAG'CTC Cuts at: 2268 2268 Size: 5362 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2578 3425 4022 4809 5077 1239 Size: 393 946 847 597 787 268 1524 Fragments arranged by size: 1524 946 847 787 597 393 268 EcoRI G'AATT_C Cuts at: 2671 3049 2671 Size: 378 4984 FspI TGC'GCA Cuts at: 2324 3868 2324 Size: 1544 3818 HaeII r_GCGC'y Cuts at: 1678 2104 2111 4509 1678 Size: 426 7 2398 2531 Fragments arranged by size: 2531 2398 426 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 4003 4035 4077 Size: 32 135 32 1052 32 1347 32 42 Cuts at: 4077 4109 1373 Size: 32 2626 Fragments arranged by size: 2626 1347 1052 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2175 2706 2952 669 Size: 988 281 237 531 246 3079 Fragments arranged by size: 3079 988 531 281 246 237 HindIII A'AGCT_T Cuts at: 748 748 Size: 5362 HpaI GTT'AAC Cuts at: 2952 2952 Size: 5362 KpnI G_GTAC'C Cuts at: 2687 2687 Size: 5362 MfeI C'AATT_G Cuts at: 2961 2961 Size: 5362 MluI A'CGCG_T Cuts at: 5238 5238 Size: 5362 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4476 4660 1305 Size: 3171 184 2007 Fragments arranged by size: 3171 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2549 2571 10 Size: 54 1425 343 717 22 2801 Fragments arranged by size: 2801 1425 717 343 54 22 MslI CAynn'nnrTG Cuts at: 518 1166 2543 3320 3679 3838 518 Size: 648 1377 777 359 159 2042 Fragments arranged by size: 2042 1377 777 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2260 2434 2778 3455 4603 4848 1606 Size: 492 162 174 344 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 492 344 245 174 162 NaeI GCC'GGC Cuts at: 2624 2624 Size: 5362 NcoI C'CATG_G Cuts at: 513 1065 2572 2581 513 Size: 552 1507 9 3294 Fragments arranged by size: 3294 1507 552 9 NdeI CA'TA_TG Cuts at: 387 387 Size: 5362 NgoMIV G'CCGG_C Cuts at: 2622 2622 Size: 5362 NheI G'CTAG_C Cuts at: 1052 2206 1052 Size: 1154 4208 NotI GC'GGCC_GC Cuts at: 2809 2809 Size: 5362 NspI r_CATG'y Cuts at: 1132 1229 2059 2720 4265 1132 Size: 97 830 661 1545 2229 Fragments arranged by size: 2229 1545 830 661 97 PasI CC'CwG_GG Cuts at: 1348 2030 1348 Size: 682 4680 PciI A'CATG_T Cuts at: 4261 4261 Size: 5362 PflMI CCAn_nnn'nTGG Cuts at: 1108 2311 1108 Size: 1203 4159 PmeI GTTT'AAAC Cuts at: 2666 2666 Size: 5362 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3431 3463 4944 4976 3431 Size: 32 1481 32 3817 Fragments arranged by size: 3817 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2611 1613 Size: 998 4364 PsiI TTA'TAA Cuts at: 2932 2932 Size: 5362 PstI C_TGCA'G Cuts at: 830 2714 830 Size: 1884 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5362 PvuII CAG'CTG Cuts at: 2260 2260 Size: 5362 SacI G_AGCT'C Cuts at: 2270 2270 Size: 5362 SacII CC_GC'GG Cuts at: 2099 2099 Size: 5362 SalI G'TCGA_C Cuts at: 1936 2704 1936 Size: 768 4594 SbfI CC_TGCA'GG Cuts at: 2714 2714 Size: 5362 ScaI AGT'ACT Cuts at: 3610 5322 3610 Size: 1712 3650 SfcI C'TryA_G Cuts at: 826 1045 2710 3845 4526 4717 826 Size: 219 1665 1135 681 191 1471 Fragments arranged by size: 1665 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2405 2577 2405 Size: 172 5190 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5362 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5362 SmaI CCC'GGG Cuts at: 1872 2689 1872 Size: 817 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2022 3466 4367 4629 820 Size: 197 926 79 1444 901 262 1553 Fragments arranged by size: 1553 1444 926 901 262 197 79 SnaBI TAC'GTA Cuts at: 493 3153 493 Size: 2660 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5362 SphI G_CATG'C Cuts at: 1132 2720 1132 Size: 1588 3774 SspI AAT'ATT Cuts at: 5 52 3286 5 Size: 47 3234 2081 Fragments arranged by size: 3234 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5362 StyI C'CwwG_G Cuts at: 513 1065 2472 2572 2581 2801 513 Size: 552 1407 100 9 220 3074 Fragments arranged by size: 3074 1407 552 220 100 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2504 2576 3389 3406 3559 3744 4189 Size: 1255 924 72 813 17 153 185 445 Cuts at: 4189 325 Size: 1498 Fragments arranged by size: 1498 1255 924 813 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3608 5320 96 Size: 274 80 33 51 1443 1631 1712 138 Fragments arranged by size: 1712 1631 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2066 2098 2519 2551 5322 5354 2066 Size: 32 421 32 2771 32 2074 Fragments arranged by size: 2771 2074 421 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5362 XbaI T'CTAG_A Cuts at: 2698 2698 Size: 5362 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2449 2570 2579 2449 Size: 121 9 5232 Fragments arranged by size: 5232 121 9 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5362 XmaI C'CCGG_G Cuts at: 1870 2687 1870 Size: 817 4545 XmnI GAAnn'nnTTC Cuts at: 3491 3491 Size: 5362 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3170 276 Size: 53 83 186 936 1636 2468 Fragments arranged by size: 2468 1636 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SanDI SapI SexAI SfoI SrfI SwaI