(Circular) (Six-base) MAPSORT of: pFN21AB7096.seq Check: 3 from: 1 to: 5284 pFN21AB7096 5284 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3094 278 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2205 2627 1937 Size: 268 422 4594 Fragments arranged by size: 4594 422 268 Acc65I G'GTAC_C Cuts at: 2605 2605 Size: 5284 AclI AA'CG_TT Cuts at: 1563 3411 3784 1563 Size: 1848 373 3063 Fragments arranged by size: 3063 1848 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3347 4731 4999 1632 Size: 1715 1384 268 1917 Fragments arranged by size: 1917 1715 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5087 AflIII A'CryG_T Cuts at: 1203 4183 5160 1203 Size: 2980 977 1327 Fragments arranged by size: 2980 1327 977 AgeI A'CCGG_T Cuts at: 4115 4115 Size: 5284 AhdI GACnn_n'nnGTC Cuts at: 4013 4013 Size: 5284 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5284 AlwNI CAG_nnn'CTG Cuts at: 3027 4599 3027 Size: 1572 3712 ApaLI G'TGCA_C Cuts at: 3341 4497 3341 Size: 1156 4128 ApoI r'AATT_y Cuts at: 1493 2593 2819 2971 5080 1493 Size: 1100 226 152 2109 1697 Fragments arranged by size: 2109 1697 1100 226 152 AseI AT'TA_AT Cuts at: 160 1032 3838 160 Size: 872 2806 1606 Fragments arranged by size: 2806 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 AvaI C'yCGr_G Cuts at: 1870 1943 2482 2609 1870 Size: 73 539 127 4545 Fragments arranged by size: 4545 539 127 73 BamHI G'GATC_C Cuts at: 2614 2981 2614 Size: 367 4917 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2059 2308 2430 2472 2605 Size: 325 217 627 272 249 122 42 133 Cuts at: 2605 4060 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 272 249 217 133 122 42 BanII G_rGCy'C Cuts at: 1391 2070 2603 1391 Size: 679 533 4072 Fragments arranged by size: 4072 679 533 BbeI G_GCGC'C Cuts at: 2063 2476 2063 Size: 413 4871 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4313 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3475 3509 1903 Size: 34 1538 34 3678 Fragments arranged by size: 3678 1538 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2136 3176 4386 2136 Size: 1040 1210 3034 Fragments arranged by size: 3034 1210 1040 BclI T'GATC_A Cuts at: 1548 1548 Size: 5284 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3895 136 Size: 107 122 71 631 605 2223 1525 Fragments arranged by size: 2223 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5279 5279 Size: 5284 BlpI GC'TnA_GC Cuts at: 2574 2701 2574 Size: 127 5157 Bme1580I G_kGCm'C Cuts at: 1790 3345 4501 1790 Size: 1555 1156 2573 Fragments arranged by size: 2573 1555 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5284 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3015 3973 442 Size: 416 53 83 399 24 1598 958 1753 Fragments arranged by size: 1753 1598 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5284 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2397 2397 Size: 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2250 2342 2392 2447 2522 3944 1239 Size: 1011 92 50 55 75 1422 2579 Fragments arranged by size: 2579 1422 1011 92 75 55 50 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3409 4274 4572 3409 Size: 865 298 4121 Fragments arranged by size: 4121 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3947 882 Size: 617 2448 2219 Fragments arranged by size: 2448 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3075 493 Size: 711 1871 2702 Fragments arranged by size: 2702 1871 711 BsaBI GATnn'nnATC Cuts at: 5278 5278 Size: 5284 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2060 2473 3091 3473 Size: 53 83 186 936 527 413 618 382 Cuts at: 3473 4108 275 Size: 635 1451 Fragments arranged by size: 1451 936 635 618 527 413 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3717 4115 4389 4536 4947 3717 Size: 398 274 147 411 4054 Fragments arranged by size: 4054 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2340 2370 1178 Size: 30 165 30 937 30 4092 Fragments arranged by size: 4092 937 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5284 BseYI C'CCAG_C Cuts at: 2070 4487 4983 2070 Size: 2417 496 2371 Fragments arranged by size: 2417 2371 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4711 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2734 3495 4523 1540 Size: 275 186 733 761 1028 2301 Fragments arranged by size: 2301 1028 761 733 275 186 BsiHKAI G_wGCw'C Cuts at: 2243 3345 3430 4501 2243 Size: 1102 85 1071 3026 Fragments arranged by size: 3026 1102 1071 85 BsmI GAATG_Cn' Cuts at: 2795 2888 2795 Size: 93 5191 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2243 2603 3345 3430 4501 1391 Size: 399 280 173 360 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 360 280 173 85 BspEI T'CCGG_A Cuts at: 4947 4947 Size: 5284 BspHI T'CATG_A Cuts at: 3173 4903 3173 Size: 1730 3554 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2639 844 Size: 1795 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3171 1394 Size: 429 1348 3507 Fragments arranged by size: 3507 1348 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3779 3953 65 Size: 1183 2531 174 1396 Fragments arranged by size: 2531 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3928 4115 1700 Size: 110 2118 187 2869 Fragments arranged by size: 2869 2118 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5284 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5284 BssSI C'ACGA_G Cuts at: 3344 4356 3344 Size: 1012 4272 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5284 BstBI TT'CG_AA Cuts at: 4088 4088 Size: 5284 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5284 BstYI r'GATC_y Cuts at: 1972 2562 2614 2981 3366 3383 4835 4921 4933 Size: 590 52 367 385 17 1452 86 12 Cuts at: 4933 5279 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 590 385 367 346 86 52 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2108 2468 513 Size: 552 939 104 360 3329 Fragments arranged by size: 3329 939 552 360 104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4545 Fragments arranged by size: 4545 683 56 BtsI GCAGTG_nn' Cuts at: 2800 3594 3614 2800 Size: 794 20 4470 Fragments arranged by size: 4470 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5284 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5249 DraI TTT'AAA Cuts at: 2588 2588 Size: 5284 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2414 4291 809 Size: 507 1098 1877 1802 Fragments arranged by size: 1877 1802 1098 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2282 2731 3620 Size: 54 1425 50 275 18 452 449 889 Cuts at: 3620 5249 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 452 449 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2731 1537 Size: 275 919 4090 Fragments arranged by size: 4090 919 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2313 3214 1353 Size: 768 192 901 3423 Fragments arranged by size: 3423 901 768 192 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3871 4245 4391 387 Size: 1064 138 2282 374 146 1280 Fragments arranged by size: 2282 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2250 2342 2392 2447 2522 3347 3944 Size: 393 618 92 50 55 75 825 597 Cuts at: 3944 4731 4999 1239 Size: 787 268 1524 Fragments arranged by size: 1524 825 787 618 597 393 268 92 75 55 50 EcoRI G'AATT_C Cuts at: 2593 2971 2593 Size: 378 4906 FspI TGC'GCA Cuts at: 3790 3790 Size: 5284 HaeII r_GCGC'y Cuts at: 1678 2063 2476 4431 1678 Size: 385 413 1955 2531 Fragments arranged by size: 2531 1955 413 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2340 2372 3925 3957 3999 Size: 32 135 32 768 32 1553 32 42 Cuts at: 3999 4031 1373 Size: 32 2626 Fragments arranged by size: 2626 1553 768 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2874 669 Size: 988 281 690 246 3079 Fragments arranged by size: 3079 988 690 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5284 HpaI GTT'AAC Cuts at: 2874 2874 Size: 5284 KasI G'GCGC_C Cuts at: 2059 2472 2059 Size: 413 4871 KpnI G_GTAC'C Cuts at: 2609 2609 Size: 5284 MfeI C'AATT_G Cuts at: 2883 2883 Size: 5284 MluI A'CGCG_T Cuts at: 5160 5160 Size: 5284 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4398 4582 1305 Size: 3093 184 2007 Fragments arranged by size: 3093 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3462 Fragments arranged by size: 3462 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3242 3601 3760 518 Size: 648 2076 359 159 2042 Fragments arranged by size: 2076 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2157 2427 2700 3377 4525 4770 1606 Size: 551 270 273 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 551 273 270 245 NarI GG'CG_CC Cuts at: 2060 2473 2060 Size: 413 4871 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3793 Fragments arranged by size: 3793 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5284 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5284 NotI GC'GGCC_GC Cuts at: 2731 2731 Size: 5284 NspI r_CATG'y Cuts at: 1132 1229 2642 4187 1132 Size: 97 1413 1545 2229 Fragments arranged by size: 2229 1545 1413 97 PasI CC'CwG_GG Cuts at: 1348 2391 1348 Size: 1043 4241 PciI A'CATG_T Cuts at: 4183 4183 Size: 5284 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5284 PfoI T'CCnGG_A Cuts at: 2227 2227 Size: 5284 PmeI GTTT'AAAC Cuts at: 2588 2588 Size: 5284 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3353 3385 4866 4898 3353 Size: 32 1481 32 3739 Fragments arranged by size: 3739 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5284 PsiI TTA'TAA Cuts at: 2854 2854 Size: 5284 PstI C_TGCA'G Cuts at: 830 2636 830 Size: 1806 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5284 PvuII CAG'CTG Cuts at: 2157 2157 Size: 5284 SalI G'TCGA_C Cuts at: 1936 2626 1936 Size: 690 4594 SbfI CC_TGCA'GG Cuts at: 2636 2636 Size: 5284 ScaI AGT'ACT Cuts at: 2133 2144 3532 5244 2133 Size: 11 1388 1712 2173 Fragments arranged by size: 2173 1712 1388 11 SfcI C'TryA_G Cuts at: 826 1045 2632 3767 4448 4639 826 Size: 219 1587 1135 681 191 1471 Fragments arranged by size: 1587 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2061 2474 2061 Size: 413 4871 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5284 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5284 SmaI CCC'GGG Cuts at: 1872 2484 2611 1872 Size: 612 127 4545 Fragments arranged by size: 4545 612 127 SmlI C'TyrA_G Cuts at: 820 1017 1943 3388 4289 4551 820 Size: 197 926 1445 901 262 1553 Fragments arranged by size: 1553 1445 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3075 493 Size: 2582 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5284 SphI G_CATG'C Cuts at: 1132 2642 1132 Size: 1510 3774 SspI AAT'ATT Cuts at: 5 52 3208 5 Size: 47 3156 2081 Fragments arranged by size: 3156 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5284 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2723 513 Size: 552 939 240 479 3074 Fragments arranged by size: 3074 939 552 479 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3311 3328 3481 3666 4111 325 Size: 1255 1731 17 153 185 445 1498 Fragments arranged by size: 1731 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2131 2142 2255 Size: 274 80 33 51 1443 154 11 113 Cuts at: 2255 2539 3530 5242 96 Size: 284 991 1712 138 Fragments arranged by size: 1712 1443 991 284 274 154 138 113 80 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5244 5276 5244 Size: 32 5252 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5284 XbaI T'CTAG_A Cuts at: 2620 2620 Size: 5284 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5284 XmaI C'CCGG_G Cuts at: 1870 2482 2609 1870 Size: 612 127 4545 Fragments arranged by size: 4545 612 127 XmnI GAAnn'nnTTC Cuts at: 3413 3413 Size: 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3092 276 Size: 53 83 186 936 1558 2468 Fragments arranged by size: 2468 1558 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI