(Circular) (Six-base) MAPSORT of: pFN21AB7095.seq Check: 517 from: 1 to: 5269 pFN21AB7095 5269 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3079 278 Size: 53 83 186 936 1543 2468 Fragments arranged by size: 2468 1543 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2612 1937 Size: 675 4594 Acc65I G'GTAC_C Cuts at: 2209 2590 2209 Size: 381 4888 AclI AA'CG_TT Cuts at: 1563 3396 3769 1563 Size: 1833 373 3063 Fragments arranged by size: 3063 1833 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3332 4716 4984 1632 Size: 1700 1384 268 1917 Fragments arranged by size: 1917 1700 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5072 AflIII A'CryG_T Cuts at: 1203 2397 4168 5145 1203 Size: 1194 1771 977 1327 Fragments arranged by size: 1771 1327 1194 977 AgeI A'CCGG_T Cuts at: 4100 4100 Size: 5269 AhdI GACnn_n'nnGTC Cuts at: 3998 3998 Size: 5269 AleI CACnn'nnGTG Cuts at: 1166 2017 1166 Size: 851 4418 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2334 1184 Size: 1150 4119 AlwNI CAG_nnn'CTG Cuts at: 3012 4584 3012 Size: 1572 3697 ApaLI G'TGCA_C Cuts at: 2495 3326 4482 2495 Size: 831 1156 3282 Fragments arranged by size: 3282 1156 831 ApoI r'AATT_y Cuts at: 1493 2578 2804 2956 5065 1493 Size: 1085 226 152 2109 1697 Fragments arranged by size: 2109 1697 1085 226 152 AseI AT'TA_AT Cuts at: 160 1032 3823 160 Size: 872 2791 1606 Fragments arranged by size: 2791 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5269 AvaI C'yCGr_G Cuts at: 1870 1943 2594 1870 Size: 73 651 4545 Fragments arranged by size: 4545 651 73 BamHI G'GATC_C Cuts at: 2599 2966 2599 Size: 367 4902 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2209 2590 4045 618 Size: 325 217 627 422 381 1455 1842 Fragments arranged by size: 1842 1455 627 422 381 325 217 BanII G_rGCy'C Cuts at: 1391 2588 1391 Size: 1197 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3460 3494 1903 Size: 34 1523 34 3678 Fragments arranged by size: 3678 1523 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3161 4371 3161 Size: 1210 4059 BclI T'GATC_A Cuts at: 1548 1548 Size: 5269 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3880 136 Size: 107 122 71 631 605 2208 1525 Fragments arranged by size: 2208 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5264 5264 Size: 5269 BlpI GC'TnA_GC Cuts at: 2335 2686 2335 Size: 351 4918 Bme1580I G_kGCm'C Cuts at: 1790 2499 3330 4486 1790 Size: 709 831 1156 2573 Fragments arranged by size: 2573 1156 831 709 BmgBI CAC'GTC Cuts at: 1318 2237 1318 Size: 919 4350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2024 2507 3000 Size: 416 53 83 399 24 607 483 493 Cuts at: 3000 3958 442 Size: 958 1753 Fragments arranged by size: 1753 958 607 493 483 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5269 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3929 1239 Size: 2690 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3394 4259 4557 3394 Size: 865 298 4106 Fragments arranged by size: 4106 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3932 882 Size: 617 2433 2219 Fragments arranged by size: 2433 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2400 3060 493 Size: 711 1196 660 2702 Fragments arranged by size: 2702 1196 711 660 BsaBI GATnn'nnATC Cuts at: 5263 5263 Size: 5269 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2391 3076 3458 4093 Size: 53 83 186 936 858 685 382 635 Cuts at: 4093 275 Size: 1451 Fragments arranged by size: 1451 936 858 685 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3702 4100 4374 4521 4932 3702 Size: 398 274 147 411 4039 Fragments arranged by size: 4039 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5044 Fragments arranged by size: 5044 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5269 BseYI C'CCAG_C Cuts at: 4472 4968 4472 Size: 496 4773 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2294 1163 Size: 573 558 4138 Fragments arranged by size: 4138 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2719 3480 4508 1540 Size: 275 186 718 761 1028 2301 Fragments arranged by size: 2301 1028 761 718 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 3330 3415 4486 2499 Size: 831 85 1071 3282 Fragments arranged by size: 3282 1071 831 85 BsmI GAATG_Cn' Cuts at: 2780 2873 2780 Size: 93 5176 BsmBI CGTCTCn'nnnn_ Cuts at: 2243 2243 Size: 5269 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2588 3330 3415 4486 1391 Size: 399 709 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 709 399 89 85 BspEI T'CCGG_A Cuts at: 4932 4932 Size: 5269 BspHI T'CATG_A Cuts at: 2361 3158 4888 2361 Size: 797 1730 2742 Fragments arranged by size: 2742 1730 797 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2197 2624 844 Size: 1353 427 3489 Fragments arranged by size: 3489 1353 427 BsrBI CCG'CTC Cuts at: 1394 1823 2334 3156 1394 Size: 429 511 822 3507 Fragments arranged by size: 3507 822 511 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2101 3764 3938 65 Size: 1183 853 1663 174 1396 Fragments arranged by size: 1663 1396 1183 853 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2415 3913 4100 1700 Size: 110 605 1498 187 2869 Fragments arranged by size: 2869 1498 605 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5269 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5269 BssSI C'ACGA_G Cuts at: 2017 3329 4341 2017 Size: 1312 1012 2945 Fragments arranged by size: 2945 1312 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5269 BstBI TT'CG_AA Cuts at: 4073 4073 Size: 5269 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5269 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2480 Size: 5269 BstYI r'GATC_y Cuts at: 1972 2599 2966 3351 3368 4820 4906 4918 5264 Size: 627 367 385 17 1452 86 12 346 Cuts at: 5264 1972 Size: 1977 Fragments arranged by size: 1977 1452 627 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2145 513 Size: 552 1080 3637 Fragments arranged by size: 3637 1080 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4530 Fragments arranged by size: 4530 683 56 BtsI GCAGTG_nn' Cuts at: 2785 3579 3599 2785 Size: 794 20 4455 Fragments arranged by size: 4455 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5269 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5234 DraI TTT'AAA Cuts at: 2573 2573 Size: 5269 DraIII CAC_nnn'GTG Cuts at: 2281 2281 Size: 5269 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4276 809 Size: 507 2960 1802 Fragments arranged by size: 2960 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2716 3605 Size: 54 1425 50 275 18 273 613 889 Cuts at: 3605 5234 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 613 275 273 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2716 1537 Size: 275 904 4090 Fragments arranged by size: 4090 904 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3199 1353 Size: 1846 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3856 4230 4376 387 Size: 1064 138 2267 374 146 1280 Fragments arranged by size: 2267 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3332 3929 4716 4984 1239 Size: 393 1700 597 787 268 1524 Fragments arranged by size: 1700 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2578 2956 2578 Size: 378 4891 EcoRV GAT'ATC Cuts at: 2194 2194 Size: 5269 FspI TGC'GCA Cuts at: 3775 3775 Size: 5269 HaeII r_GCGC'y Cuts at: 1678 4416 1678 Size: 2738 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3910 3942 3984 4016 1373 Size: 32 135 32 2338 32 42 32 2626 Fragments arranged by size: 2626 2338 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2613 2859 669 Size: 988 281 675 246 3079 Fragments arranged by size: 3079 988 675 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5269 HpaI GTT'AAC Cuts at: 2859 2859 Size: 5269 KpnI G_GTAC'C Cuts at: 2213 2594 2213 Size: 381 4888 MfeI C'AATT_G Cuts at: 2474 2868 2474 Size: 394 4875 MluI A'CGCG_T Cuts at: 5145 5145 Size: 5269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2452 4383 4567 1305 Size: 1147 1931 184 2007 Fragments arranged by size: 2007 1931 1147 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3447 Fragments arranged by size: 3447 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2017 3227 3586 3745 518 Size: 648 851 1210 359 159 2042 Fragments arranged by size: 2042 1210 851 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2058 2339 2439 2685 3362 4510 4755 1606 Size: 452 281 100 246 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 452 281 246 245 100 NaeI GCC'GGC Cuts at: 2417 2417 Size: 5269 NcoI C'CATG_G Cuts at: 513 1065 2145 513 Size: 552 1080 3637 Fragments arranged by size: 3637 1080 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5269 NgoMIV G'CCGG_C Cuts at: 2415 2415 Size: 5269 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5269 NotI GC'GGCC_GC Cuts at: 2716 2716 Size: 5269 NspI r_CATG'y Cuts at: 1132 1229 2206 2627 4172 1132 Size: 97 977 421 1545 2229 Fragments arranged by size: 2229 1545 977 421 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5269 PciI A'CATG_T Cuts at: 4168 4168 Size: 5269 PflMI CCAn_nnn'nTGG Cuts at: 1108 2281 1108 Size: 1173 4096 PmeI GTTT'AAAC Cuts at: 2573 2573 Size: 5269 PmlI CAC'GTG Cuts at: 2400 2400 Size: 5269 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3338 3370 4851 4883 3338 Size: 32 1481 32 3724 Fragments arranged by size: 3724 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5269 PsiI TTA'TAA Cuts at: 2839 2839 Size: 5269 PstI C_TGCA'G Cuts at: 830 2621 830 Size: 1791 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5269 PvuII CAG'CTG Cuts at: 2339 2339 Size: 5269 SalI G'TCGA_C Cuts at: 1936 2611 1936 Size: 675 4594 SbfI CC_TGCA'GG Cuts at: 2621 2621 Size: 5269 ScaI AGT'ACT Cuts at: 3517 5229 3517 Size: 1712 3557 SfcI C'TryA_G Cuts at: 826 1045 2617 3752 4433 4624 826 Size: 219 1572 1135 681 191 1471 Fragments arranged by size: 1572 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5269 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5269 SmaI CCC'GGG Cuts at: 1872 2596 1872 Size: 724 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3373 4274 4536 820 Size: 197 926 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3060 493 Size: 2567 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5269 SphI G_CATG'C Cuts at: 1132 2627 1132 Size: 1495 3774 SspI AAT'ATT Cuts at: 5 52 3193 5 Size: 47 3141 2081 Fragments arranged by size: 3141 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5269 StyI C'CwwG_G Cuts at: 513 1065 2145 2708 513 Size: 552 1080 563 3074 Fragments arranged by size: 3074 1080 563 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3296 3313 3466 3651 4096 325 Size: 1255 1716 17 153 185 445 1498 Fragments arranged by size: 1716 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2199 3515 5227 Size: 274 80 33 51 1443 222 1316 1712 Cuts at: 5227 96 Size: 138 Fragments arranged by size: 1712 1443 1316 274 222 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2363 2395 5229 5261 2363 Size: 32 2834 32 2371 Fragments arranged by size: 2834 2371 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2407 1316 Size: 1091 4178 XbaI T'CTAG_A Cuts at: 2605 2605 Size: 5269 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2383 2383 Size: 5269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5269 XmaI C'CCGG_G Cuts at: 1870 2594 1870 Size: 724 4545 XmnI GAAnn'nnTTC Cuts at: 3398 3398 Size: 5269 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3077 276 Size: 53 83 186 936 1543 2468 Fragments arranged by size: 2468 1543 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI