(Circular) (Six-base) MAPSORT of: pFN21AB7094.seq Check: 2063 from: 1 to: 5263 pFN21AB7094 5263 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2520 2520 Size: 5263 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3073 278 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2606 1937 Size: 669 4594 Acc65I G'GTAC_C Cuts at: 2584 2584 Size: 5263 AclI AA'CG_TT Cuts at: 1563 3390 3763 1563 Size: 1827 373 3063 Fragments arranged by size: 3063 1827 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 1999 2228 3326 4710 4978 1632 Size: 367 229 1098 1384 268 1917 Fragments arranged by size: 1917 1384 1098 367 268 229 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5066 AflIII A'CryG_T Cuts at: 1203 2439 4162 5139 1203 Size: 1236 1723 977 1327 Fragments arranged by size: 1723 1327 1236 977 AgeI A'CCGG_T Cuts at: 4094 4094 Size: 5263 AhdI GACnn_n'nnGTC Cuts at: 3992 3992 Size: 5263 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5263 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 1184 Size: 948 4315 AlwNI CAG_nnn'CTG Cuts at: 2523 3006 4578 2523 Size: 483 1572 3208 Fragments arranged by size: 3208 1572 483 ApaLI G'TGCA_C Cuts at: 3320 4476 3320 Size: 1156 4107 ApoI r'AATT_y Cuts at: 1493 2572 2798 2950 5059 1493 Size: 1079 226 152 2109 1697 Fragments arranged by size: 2109 1697 1079 226 152 AseI AT'TA_AT Cuts at: 160 1032 3817 160 Size: 872 2785 1606 Fragments arranged by size: 2785 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 AvaI C'yCGr_G Cuts at: 1870 1943 2366 2588 1870 Size: 73 423 222 4545 Fragments arranged by size: 4545 423 222 73 BamHI G'GATC_C Cuts at: 2593 2960 2593 Size: 367 4896 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2584 4039 618 Size: 325 217 627 797 1455 1842 Fragments arranged by size: 1842 1455 797 627 325 217 BanII G_rGCy'C Cuts at: 1391 2499 2582 1391 Size: 1108 83 4072 Fragments arranged by size: 4072 1108 83 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4292 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3454 3488 1903 Size: 34 1517 34 3678 Fragments arranged by size: 3678 1517 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3155 4365 3155 Size: 1210 4053 BclI T'GATC_A Cuts at: 1548 1548 Size: 5263 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 3874 136 Size: 107 122 71 631 605 631 1571 1525 Fragments arranged by size: 1571 1525 631 631 605 122 107 71 BglII A'GATC_T Cuts at: 5258 5258 Size: 5263 BlpI GC'TnA_GC Cuts at: 2680 2680 Size: 5263 Bme1580I G_kGCm'C Cuts at: 1790 3324 4480 1790 Size: 1534 1156 2573 Fragments arranged by size: 2573 1534 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2994 3952 442 Size: 416 53 83 399 24 1577 958 1753 Fragments arranged by size: 1753 1577 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5263 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2049 2049 Size: 5263 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3923 1239 Size: 2684 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2168 3388 4253 4551 2168 Size: 1220 865 298 2880 Fragments arranged by size: 2880 1220 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3926 882 Size: 617 2427 2219 Fragments arranged by size: 2427 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2440 3054 493 Size: 711 1236 614 2702 Fragments arranged by size: 2702 1236 711 614 BsaBI GATnn'nnATC Cuts at: 5257 5257 Size: 5263 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2069 3070 3452 4087 Size: 53 83 186 936 536 1001 382 635 Cuts at: 4087 275 Size: 1451 Fragments arranged by size: 1451 1001 936 635 536 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3696 4094 4368 4515 4926 3696 Size: 398 274 147 411 4033 Fragments arranged by size: 4033 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5038 Fragments arranged by size: 5038 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2264 1186 Size: 1078 4185 BseYI C'CCAG_C Cuts at: 2212 2239 4466 4962 2212 Size: 27 2227 496 2513 Fragments arranged by size: 2513 2227 496 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 1163 Size: 573 633 4057 Fragments arranged by size: 4057 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2307 2341 2428 2713 3474 4502 Size: 275 186 306 34 87 285 761 1028 Cuts at: 4502 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 306 285 275 186 87 34 BsiHKAI G_wGCw'C Cuts at: 3324 3409 4480 3324 Size: 85 1071 4107 Fragments arranged by size: 4107 1071 85 BsmI GAATG_Cn' Cuts at: 2774 2867 2774 Size: 93 5170 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2582 3324 3409 4480 1391 Size: 399 709 83 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 709 399 85 83 BspEI T'CCGG_A Cuts at: 4926 4926 Size: 5263 BspHI T'CATG_A Cuts at: 3152 4882 3152 Size: 1730 3533 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2388 2520 2618 844 Size: 1544 132 98 3489 Fragments arranged by size: 3489 1544 132 98 BsrBI CCG'CTC Cuts at: 1394 1823 2505 3150 1394 Size: 429 682 645 3507 Fragments arranged by size: 3507 682 645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3758 3932 65 Size: 1183 2510 174 1396 Fragments arranged by size: 2510 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2340 3907 4094 1700 Size: 110 530 1567 187 2869 Fragments arranged by size: 2869 1567 530 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5263 BssHII G'CGCG_C Cuts at: 1925 2285 1925 Size: 360 4903 BssSI C'ACGA_G Cuts at: 3323 4335 3323 Size: 1012 4251 BstAPI GCAn_nnn'nTGC Cuts at: 794 2523 794 Size: 1729 3534 BstBI TT'CG_AA Cuts at: 4067 4067 Size: 5263 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5263 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2080 2029 Size: 51 5212 BstYI r'GATC_y Cuts at: 1972 2520 2593 2960 3345 3362 4814 4900 4912 Size: 548 73 367 385 17 1452 86 12 Cuts at: 4912 5258 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2394 2422 513 Size: 552 939 390 28 3354 Fragments arranged by size: 3354 939 552 390 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4524 Fragments arranged by size: 4524 683 56 BtsI GCAGTG_nn' Cuts at: 2085 2116 2779 3573 3593 2085 Size: 31 663 794 20 3755 Fragments arranged by size: 3755 794 663 31 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5263 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5228 DraI TTT'AAA Cuts at: 2567 2567 Size: 5263 DraIII CAC_nnn'GTG Cuts at: 2323 2323 Size: 5263 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2416 4270 809 Size: 507 1100 1854 1802 Fragments arranged by size: 1854 1802 1100 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2085 2295 2304 Size: 54 1425 50 275 18 255 210 9 Cuts at: 2304 2352 2425 2446 2710 3599 5228 8 Size: 48 73 21 264 889 1629 43 Fragments arranged by size: 1629 1425 889 275 264 255 210 73 54 50 48 43 21 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2304 2425 2710 1537 Size: 275 492 121 285 4090 Fragments arranged by size: 4090 492 285 275 121 EarI CTCTTCn'nnn_ Cuts at: 1353 3193 1353 Size: 1840 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2264 3850 4224 4370 387 Size: 1064 138 675 1586 374 146 1280 Fragments arranged by size: 1586 1280 1064 675 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1999 2228 3326 3923 4710 4978 1239 Size: 393 367 229 1098 597 787 268 1524 Fragments arranged by size: 1524 1098 787 597 393 367 268 229 EcoNI CCTnn'n_nnAGG Cuts at: 2195 2195 Size: 5263 EcoO109I rG'GnC_Cy Cuts at: 2399 2530 2399 Size: 131 5132 EcoRI G'AATT_C Cuts at: 2572 2950 2572 Size: 378 4885 FspI TGC'GCA Cuts at: 2516 3769 2516 Size: 1253 4010 HaeII r_GCGC'y Cuts at: 1678 2261 2272 4410 1678 Size: 583 11 2138 2531 Fragments arranged by size: 2531 2138 583 11 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3904 3936 3978 4010 1373 Size: 32 135 32 2332 32 42 32 2626 Fragments arranged by size: 2626 2332 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2607 2853 669 Size: 988 281 669 246 3079 Fragments arranged by size: 3079 988 669 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5263 HpaI GTT'AAC Cuts at: 2853 2853 Size: 5263 KpnI G_GTAC'C Cuts at: 2588 2588 Size: 5263 MfeI C'AATT_G Cuts at: 2862 2862 Size: 5263 MluI A'CGCG_T Cuts at: 5139 5139 Size: 5263 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4377 4561 1305 Size: 3072 184 2007 Fragments arranged by size: 3072 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 2976 Fragments arranged by size: 2976 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3221 3580 3739 518 Size: 648 2055 359 159 2042 Fragments arranged by size: 2055 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2239 2679 3356 4504 4749 1606 Size: 633 440 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 633 440 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3772 Fragments arranged by size: 3772 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5263 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5263 NotI GC'GGCC_GC Cuts at: 2710 2710 Size: 5263 NspI r_CATG'y Cuts at: 1132 1229 2621 4166 1132 Size: 97 1392 1545 2229 Fragments arranged by size: 2229 1545 1392 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5263 PciI A'CATG_T Cuts at: 4162 4162 Size: 5263 PflMI CCAn_nnn'nTGG Cuts at: 1108 2323 2395 1108 Size: 1215 72 3976 Fragments arranged by size: 3976 1215 72 PmeI GTTT'AAAC Cuts at: 2567 2567 Size: 5263 PmlI CAC'GTG Cuts at: 2440 2440 Size: 5263 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3332 3364 4845 4877 3332 Size: 32 1481 32 3718 Fragments arranged by size: 3718 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2399 2399 Size: 5263 PshAI GACnn'nnGTC Cuts at: 1613 2306 1613 Size: 693 4570 PsiI TTA'TAA Cuts at: 2833 2833 Size: 5263 PspXI vC'TCGA_Gb Cuts at: 2366 2366 Size: 5263 PstI C_TGCA'G Cuts at: 830 2040 2615 830 Size: 1210 575 3478 Fragments arranged by size: 3478 1210 575 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5263 PvuII CAG'CTG Cuts at: 2239 2239 Size: 5263 SalI G'TCGA_C Cuts at: 1936 2605 1936 Size: 669 4594 SanDI GG'GwC_CC Cuts at: 2399 2399 Size: 5263 SbfI CC_TGCA'GG Cuts at: 2615 2615 Size: 5263 ScaI AGT'ACT Cuts at: 3511 5223 3511 Size: 1712 3551 SexAI A'CCwGG_T Cuts at: 2190 2319 2190 Size: 129 5134 SfcI C'TryA_G Cuts at: 826 1045 2036 2611 3746 4427 4618 826 Size: 219 991 575 1135 681 191 1471 Fragments arranged by size: 1471 1135 991 681 575 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 5263 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5263 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5263 SmaI CCC'GGG Cuts at: 1872 2590 1872 Size: 718 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2183 2366 3367 4268 4530 820 Size: 197 926 240 183 1001 901 262 1553 Fragments arranged by size: 1553 1001 926 901 262 240 197 183 SnaBI TAC'GTA Cuts at: 493 3054 493 Size: 2561 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5263 SphI G_CATG'C Cuts at: 1132 2621 1132 Size: 1489 3774 SspI AAT'ATT Cuts at: 5 52 3187 5 Size: 47 3135 2081 Fragments arranged by size: 3135 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5263 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2702 513 Size: 552 939 69 629 3074 Fragments arranged by size: 3074 939 629 552 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3290 3307 3460 3645 4090 325 Size: 1255 1710 17 153 185 445 1498 Fragments arranged by size: 1710 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3509 5221 96 Size: 274 80 33 51 1443 1532 1712 138 Fragments arranged by size: 1712 1532 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5223 5255 5223 Size: 32 5231 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5263 XbaI T'CTAG_A Cuts at: 2599 2599 Size: 5263 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2080 2080 Size: 5263 XhoI C'TCGA_G Cuts at: 1943 2366 1943 Size: 423 4840 XmaI C'CCGG_G Cuts at: 1870 2588 1870 Size: 718 4545 XmnI GAAnn'nnTTC Cuts at: 3392 3392 Size: 5263 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3071 276 Size: 53 83 186 936 1537 2468 Fragments arranged by size: 2468 1537 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI