Restriction map for
pFN21AB7092
(Circular) (Six-base) MAPSORT of: pFN21AB7092.seq Check: 9342 from: 1 to: 5257
pFN21AB7092 5257 bp
With 182 enzymes: SgfI *
January 16, 2009 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2038 2038
Size: 5257
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3067 278
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2600 1937
Size: 663 4594
Acc65I G'GTAC_C
Cuts at: 2578 2578
Size: 5257
AclI AA'CG_TT
Cuts at: 1563 3384 3757 1563
Size: 1821 373 3063
Fragments arranged by size:
3063 1821 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2063 2360 3320 4704 4972 1632
Size: 431 297 960 1384 268 1917
Fragments arranged by size:
1917 1384 960 431 297 268
AflII C'TTAA_G
Cuts at: 820 1017 2018 820
Size: 197 1001 4059
Fragments arranged by size:
4059 1001 197
AflIII A'CryG_T
Cuts at: 1203 2413 4156 5133 1203
Size: 1210 1743 977 1327
Fragments arranged by size:
1743 1327 1210 977
AgeI A'CCGG_T
Cuts at: 4088 4088
Size: 5257
AhdI GACnn_n'nnGTC
Cuts at: 3986 3986
Size: 5257
AleI CACnn'nnGTG
Cuts at: 1166 2483 1166
Size: 1317 3940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5257
AlwNI CAG_nnn'CTG
Cuts at: 2038 3000 4572 2038
Size: 962 1572 2723
Fragments arranged by size:
2723 1572 962
ApaI G_GGCC'C
Cuts at: 2423 2423
Size: 5257
ApaLI G'TGCA_C
Cuts at: 3314 4470 3314
Size: 1156 4101
ApoI r'AATT_y
Cuts at: 1493 2566 2792 2944 5053 1493
Size: 1073 226 152 2109 1697
Fragments arranged by size:
2109 1697 1073 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3811 160
Size: 872 2779 1606
Fragments arranged by size:
2779 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
AvaI C'yCGr_G
Cuts at: 1870 1943 2582 1870
Size: 73 639 4545
Fragments arranged by size:
4545 639 73
BamHI G'GATC_C
Cuts at: 2587 2954 2587
Size: 367 4890
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2184 2269 2578 4033 618
Size: 325 217 627 397 85 309 1455 1842
Fragments arranged by size:
1842 1455 627 397 325 309 217 85
BanII G_rGCy'C
Cuts at: 1391 2295 2423 2576 1391
Size: 904 128 153 4072
Fragments arranged by size:
4072 904 153 128
BbeI G_GCGC'C
Cuts at: 2188 2188
Size: 5257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4286
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3448 3482 1903
Size: 34 1511 34 3678
Fragments arranged by size:
3678 1511 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3149 4359 3149
Size: 1210 4047
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5257
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3868 136
Size: 107 122 71 631 605 2196 1525
Fragments arranged by size:
2196 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5252 5252
Size: 5257
BlpI GC'TnA_GC
Cuts at: 2156 2674 2156
Size: 518 4739
Bme1580I G_kGCm'C
Cuts at: 1790 2274 2409 2423 3318 4474 1790
Size: 484 135 14 895 1156 2573
Fragments arranged by size:
2573 1156 895 484 135 14
BmgBI CAC'GTC
Cuts at: 1318 2428 1318
Size: 1110 4147
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2074 2988 3946
Size: 416 53 83 399 24 657 914 958
Cuts at: 3946 442
Size: 1753
Fragments arranged by size:
1753 958 914 657 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2309 3917 1239
Size: 1070 1608 2579
Fragments arranged by size:
2579 1608 1070
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2336 3382 4247 4545 2336
Size: 1046 865 298 3048
Fragments arranged by size:
3048 1046 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3920 882
Size: 617 2421 2219
Fragments arranged by size:
2421 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2410 2512 3048 493
Size: 711 1206 102 536 2702
Fragments arranged by size:
2702 1206 711 536 102
BsaBI GATnn'nnATC
Cuts at: 5251 5251
Size: 5257
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2185 3064 3446 4081
Size: 53 83 186 936 652 879 382 635
Cuts at: 4081 275
Size: 1451
Fragments arranged by size:
1451 936 879 652 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3690 4088 4362 4509 4920 3690
Size: 398 274 147 411 4027
Fragments arranged by size:
4027 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5032
Fragments arranged by size:
5032 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2339 2543 1186
Size: 1153 204 3900
Fragments arranged by size:
3900 1153 204
BseYI C'CCAG_C
Cuts at: 4460 4956 4460
Size: 496 4761
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 1163
Size: 573 336 4348
Fragments arranged by size:
4348 573 336
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 2707 3468 4496 1540
Size: 275 186 234 472 761 1028 2301
Fragments arranged by size:
2301 1028 761 472 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2349 3318 3403 4474 2295
Size: 54 969 85 1071 3078
Fragments arranged by size:
3078 1071 969 85 54
BsiWI C'GTAC_G
Cuts at: 2410 2410
Size: 5257
BsmI GAATG_Cn'
Cuts at: 2768 2861 2768
Size: 93 5164
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2274 2295 2349 2409 2423 2576 3318
Size: 399 484 21 54 60 14 153 742
Cuts at: 3318 3403 4474 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 484 399 153 85 60
54 21 14
BspEI T'CCGG_A
Cuts at: 4920 4920
Size: 5257
BspHI T'CATG_A
Cuts at: 3146 4876 3146
Size: 1730 3527
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2038 2094 2205 2612 844
Size: 1194 56 111 407 3489
Fragments arranged by size:
3489 1194 407 111 56
BsrBI CCG'CTC
Cuts at: 1394 1823 3144 1394
Size: 429 1321 3507
Fragments arranged by size:
3507 1321 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3752 3926 65
Size: 1183 2504 174 1396
Fragments arranged by size:
2504 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2115 3901 4088 1700
Size: 110 305 1786 187 2869
Fragments arranged by size:
2869 1786 305 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5257
BssHII G'CGCG_C
Cuts at: 1925 2224 1925
Size: 299 4958
BssSI C'ACGA_G
Cuts at: 2027 2090 3317 4329 2027
Size: 63 1227 1012 2955
Fragments arranged by size:
2955 1227 1012 63
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2038 2095 794
Size: 1244 57 3956
Fragments arranged by size:
3956 1244 57
BstBI TT'CG_AA
Cuts at: 4061 4061
Size: 5257
BstEII G'GTnAC_C
Cuts at: 1184 2071 2431 1184
Size: 887 360 4010
Fragments arranged by size:
4010 887 360
BstYI r'GATC_y
Cuts at: 1972 2587 2954 3339 3356 4808 4894 4906 5252
Size: 615 367 385 17 1452 86 12 346
Cuts at: 5252 1972
Size: 1977
Fragments arranged by size:
1977 1452 615 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2385 513
Size: 552 939 381 3385
Fragments arranged by size:
3385 939 552 381
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2550 487
Size: 683 56 1324 3194
Fragments arranged by size:
3194 1324 683 56
BtsI GCAGTG_nn'
Cuts at: 2773 3567 3587 2773
Size: 794 20 4443
Fragments arranged by size:
4443 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5222
DraI TTT'AAA
Cuts at: 2561 2561
Size: 5257
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4264 809
Size: 507 2948 1802
Fragments arranged by size:
2948 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2704 3593
Size: 54 1425 50 275 18 402 472 889
Cuts at: 3593 5222 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 472 402 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 2704 1537
Size: 275 420 472 4090
Fragments arranged by size:
4090 472 420 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3187 1353
Size: 1834 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3844 4218 4364 387
Size: 1064 138 2255 374 146 1280
Fragments arranged by size:
2255 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2293
Size: 5257
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2309 2360 3320 3917 4704 4972
Size: 393 431 246 51 960 597 787 268
Cuts at: 4972 1239
Size: 1524
Fragments arranged by size:
1524 960 787 597 431 393 268 246
51
EcoO109I rG'GnC_Cy
Cuts at: 2420 2420
Size: 5257
EcoRI G'AATT_C
Cuts at: 2566 2944 2566
Size: 378 4879
FspI TGC'GCA
Cuts at: 2360 3763 2360
Size: 1403 3854
HaeII r_GCGC'y
Cuts at: 1678 2188 4404 1678
Size: 510 2216 2531
Fragments arranged by size:
2531 2216 510
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3898 3930 3972 4004 1373
Size: 32 135 32 2326 32 42 32 2626
Fragments arranged by size:
2626 2326 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2601 2847 669
Size: 988 281 663 246 3079
Fragments arranged by size:
3079 988 663 281 246
HindIII A'AGCT_T
Cuts at: 748 2015 748
Size: 1267 3990
HpaI GTT'AAC
Cuts at: 2847 2847
Size: 5257
KasI G'GCGC_C
Cuts at: 2184 2184
Size: 5257
KpnI G_GTAC'C
Cuts at: 2582 2582
Size: 5257
MfeI C'AATT_G
Cuts at: 2856 2856
Size: 5257
MluI A'CGCG_T
Cuts at: 2413 5133 2413
Size: 2720 2537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4371 4555 1305
Size: 3066 184 2007
Fragments arranged by size:
3066 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3435
Fragments arranged by size:
3435 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 3215 3574 3733 518
Size: 648 1317 732 359 159 2042
Fragments arranged by size:
2042 1317 732 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2125 2155 2673 3350 4498 4743 1606
Size: 519 30 518 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 519 518 245 30
NarI GG'CG_CC
Cuts at: 2185 2185
Size: 5257
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3766
Fragments arranged by size:
3766 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5257
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5257
NotI GC'GGCC_GC
Cuts at: 2704 2704
Size: 5257
NspI r_CATG'y
Cuts at: 1132 1229 2615 4160 1132
Size: 97 1386 1545 2229
Fragments arranged by size:
2229 1545 1386 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5257
PciI A'CATG_T
Cuts at: 4156 4156
Size: 5257
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5257
PmeI GTTT'AAAC
Cuts at: 2561 2561
Size: 5257
PmlI CAC'GTG
Cuts at: 2512 2512
Size: 5257
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3326 3358 4839 4871 3326
Size: 32 1481 32 3712
Fragments arranged by size:
3712 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5257
PsiI TTA'TAA
Cuts at: 2827 2827
Size: 5257
PspOMI G'GGCC_C
Cuts at: 2419 2419
Size: 5257
PstI C_TGCA'G
Cuts at: 830 2124 2609 830
Size: 1294 485 3478
Fragments arranged by size:
3478 1294 485
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5257
PvuII CAG'CTG
Cuts at: 2155 2155
Size: 5257
SacI G_AGCT'C
Cuts at: 2295 2295
Size: 5257
SalI G'TCGA_C
Cuts at: 1936 2599 1936
Size: 663 4594
SbfI CC_TGCA'GG
Cuts at: 2609 2609
Size: 5257
ScaI AGT'ACT
Cuts at: 3505 5217 3505
Size: 1712 3545
SfcI C'TryA_G
Cuts at: 826 1045 2120 2605 3740 4421 4612 826
Size: 219 1075 485 1135 681 191 1471
Fragments arranged by size:
1471 1135 1075 681 485 219 191
SfoI GGC'GCC
Cuts at: 2186 2186
Size: 5257
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5257
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5257
SmaI CCC'GGG
Cuts at: 1872 2584 1872
Size: 712 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2018 2351 3361 4262 4524 820
Size: 197 926 75 333 1010 901 262 1553
Fragments arranged by size:
1553 1010 926 901 333 262 197 75
SnaBI TAC'GTA
Cuts at: 493 3048 493
Size: 2555 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5257
SphI G_CATG'C
Cuts at: 1132 2615 1132
Size: 1483 3774
SspI AAT'ATT
Cuts at: 5 52 3181 5
Size: 47 3129 2081
Fragments arranged by size:
3129 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5257
StyI C'CwwG_G
Cuts at: 513 1065 2004 2696 513
Size: 552 939 692 3074
Fragments arranged by size:
3074 939 692 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2473 3284 3301 3454 3639 4084 325
Size: 1255 893 811 17 153 185 445 1498
Fragments arranged by size:
1498 1255 893 811 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2520 3503 5215
Size: 274 80 33 51 1443 543 983 1712
Cuts at: 5215 96
Size: 138
Fragments arranged by size:
1712 1443 983 543 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5217 5249 5217
Size: 32 5225
Tth111I GACn'n_nGTC
Cuts at: 1316 2303 2474 1316
Size: 987 171 4099
Fragments arranged by size:
4099 987 171
XbaI T'CTAG_A
Cuts at: 2593 2593
Size: 5257
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5257
XmaI C'CCGG_G
Cuts at: 1870 2582 1870
Size: 712 4545
XmnI GAAnn'nnTTC
Cuts at: 3386 3386
Size: 5257
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3065 276
Size: 53 83 186 936 1531 2468
Fragments arranged by size:
2468 1531 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
Bpu10I BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PpuMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SrfI SwaI XcmI