(Circular) (Six-base) MAPSORT of: pFN21AB7065.seq Check: 5304 from: 1 to: 7054 pFN21AB7065 7054 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4864 278 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4397 1937 Size: 2460 4594 Acc65I G'GTAC_C Cuts at: 4375 4375 Size: 7054 AclI AA'CG_TT Cuts at: 1563 5181 5554 1563 Size: 3618 373 3063 Fragments arranged by size: 3618 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2574 4134 5117 6501 6769 1632 Size: 942 1560 983 1384 268 1917 Fragments arranged by size: 1917 1560 1384 983 942 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6857 AflIII A'CryG_T Cuts at: 1203 2152 5953 6930 1203 Size: 949 3801 977 1327 Fragments arranged by size: 3801 1327 977 949 AgeI A'CCGG_T Cuts at: 5885 5885 Size: 7054 AhdI GACnn_n'nnGTC Cuts at: 5783 5783 Size: 7054 AleI CACnn'nnGTG Cuts at: 1166 3831 1166 Size: 2665 4389 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7054 AlwNI CAG_nnn'CTG Cuts at: 4797 6369 4797 Size: 1572 5482 ApaLI G'TGCA_C Cuts at: 5111 6267 5111 Size: 1156 5898 ApoI r'AATT_y Cuts at: 1493 2125 2264 2272 2376 2392 2460 2796 2880 Size: 632 139 8 104 16 68 336 84 Cuts at: 2880 2964 3132 3216 3300 3384 3468 3587 3746 Size: 84 168 84 84 84 84 119 159 Cuts at: 3746 3888 4056 4140 4308 4363 4589 4741 6850 Size: 142 168 84 168 55 226 152 2109 Cuts at: 6850 1493 Size: 1697 Fragments arranged by size: 2109 1697 632 336 226 168 168 168 159 152 142 139 119 104 84 84 84 84 84 84 84 68 55 16 8 AseI AT'TA_AT Cuts at: 160 1032 5608 160 Size: 872 4576 1606 Fragments arranged by size: 4576 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 AvaI C'yCGr_G Cuts at: 1870 1943 4379 1870 Size: 73 2436 4545 Fragments arranged by size: 4545 2436 73 BamHI G'GATC_C Cuts at: 4384 4751 4384 Size: 367 6687 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4375 5830 618 Size: 325 217 627 2588 1455 1842 Fragments arranged by size: 2588 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4373 1391 Size: 2982 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2299 3351 928 Size: 971 400 1052 4631 Fragments arranged by size: 4631 1052 971 400 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5245 5279 1903 Size: 34 3308 34 3678 Fragments arranged by size: 3678 3308 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4946 6156 4946 Size: 1210 5844 BclI T'GATC_A Cuts at: 1548 1548 Size: 7054 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5665 136 Size: 107 122 71 631 605 3993 1525 Fragments arranged by size: 3993 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7049 7049 Size: 7054 BlpI GC'TnA_GC Cuts at: 4471 4471 Size: 7054 Bme1580I G_kGCm'C Cuts at: 1790 2175 5115 6271 1790 Size: 385 2940 1156 2573 Fragments arranged by size: 2940 2573 1156 385 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7054 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2015 4785 5743 Size: 416 53 83 399 24 598 2770 958 Cuts at: 5743 442 Size: 1753 Fragments arranged by size: 2770 1753 958 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7054 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2747 2915 3755 2747 Size: 168 840 6046 Fragments arranged by size: 6046 840 168 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2490 2658 2742 2910 3078 3246 3330 3414 Size: 1251 168 84 168 168 168 84 84 Cuts at: 3414 3498 3666 3750 3834 4002 4086 4254 4338 Size: 84 168 84 84 168 84 168 84 Cuts at: 4338 5714 1239 Size: 1376 2579 Fragments arranged by size: 2579 1376 1251 168 168 168 168 168 168 168 84 84 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5179 6044 6342 5179 Size: 865 298 5891 Fragments arranged by size: 5891 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5717 882 Size: 617 4218 2219 Fragments arranged by size: 4218 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4845 493 Size: 711 3641 2702 Fragments arranged by size: 3641 2702 711 BsaBI GATnn'nnATC Cuts at: 7048 7048 Size: 7054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4861 5243 5878 275 Size: 53 83 186 936 3328 382 635 1451 Fragments arranged by size: 3328 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5487 5885 6159 6306 6717 5487 Size: 398 274 147 411 5824 Fragments arranged by size: 5824 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6829 Fragments arranged by size: 6829 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3937 1186 Size: 2751 4303 BseYI C'CCAG_C Cuts at: 2804 6257 6753 2804 Size: 3453 496 3105 Fragments arranged by size: 3453 3105 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2937 3939 4029 1163 Size: 573 1201 1002 90 4188 Fragments arranged by size: 4188 1201 1002 573 90 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4504 5265 6293 1540 Size: 275 186 2503 761 1028 2301 Fragments arranged by size: 2503 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5115 5200 6271 5115 Size: 85 1071 5898 Fragments arranged by size: 5898 1071 85 BsmI GAATG_Cn' Cuts at: 4565 4658 4565 Size: 93 6961 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 4373 5115 5200 6271 1391 Size: 399 385 2198 742 85 1071 2174 Fragments arranged by size: 2198 2174 1071 742 399 385 85 BspEI T'CCGG_A Cuts at: 6717 6717 Size: 7054 BspHI T'CATG_A Cuts at: 4943 6673 4943 Size: 1730 5324 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4409 844 Size: 3565 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4941 1394 Size: 429 3118 3507 Fragments arranged by size: 3507 3118 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5549 5723 65 Size: 1183 4301 174 1396 Fragments arranged by size: 4301 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5698 5885 1700 Size: 110 3888 187 2869 Fragments arranged by size: 3888 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7054 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7054 BssSI C'ACGA_G Cuts at: 5114 6126 5114 Size: 1012 6042 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7054 BstBI TT'CG_AA Cuts at: 5858 5858 Size: 7054 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7054 BstYI r'GATC_y Cuts at: 1972 4384 4751 5136 5153 6605 6691 6703 7049 Size: 2412 367 385 17 1452 86 12 346 Cuts at: 7049 1972 Size: 1977 Fragments arranged by size: 2412 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5563 Fragments arranged by size: 5563 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6315 Fragments arranged by size: 6315 683 56 BtsI GCAGTG_nn' Cuts at: 4570 5364 5384 4570 Size: 794 20 6240 Fragments arranged by size: 6240 794 20 ClaI AT'CG_AT Cuts at: 1554 3271 1554 Size: 1717 5337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7019 DraI TTT'AAA Cuts at: 2596 3604 4358 2596 Size: 1008 754 5292 Fragments arranged by size: 5292 1008 754 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6061 809 Size: 507 4745 1802 Fragments arranged by size: 4745 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 4501 5390 Size: 54 1425 50 275 18 224 2447 889 Cuts at: 5390 7019 8 Size: 1629 43 Fragments arranged by size: 2447 1629 1425 889 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4501 1537 Size: 275 2689 4090 Fragments arranged by size: 4090 2689 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2550 2970 4984 1353 Size: 1197 420 2014 3423 Fragments arranged by size: 3423 2014 1197 420 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5641 6015 6161 387 Size: 1064 138 4052 374 146 1280 Fragments arranged by size: 4052 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2490 2574 2658 2742 2910 3078 3246 Size: 393 858 84 84 84 168 168 168 Cuts at: 3246 3330 3414 3498 3666 3750 3834 4002 4086 Size: 84 84 84 168 84 84 168 84 Cuts at: 4086 4134 4254 4338 5117 5714 6501 6769 1239 Size: 48 120 84 779 597 787 268 1524 Fragments arranged by size: 1524 858 787 779 597 393 268 168 168 168 168 168 120 84 84 84 84 84 84 84 84 84 84 48 EcoRI G'AATT_C Cuts at: 2376 2392 2460 2796 3468 3888 4363 4741 2376 Size: 16 68 336 672 420 475 378 4689 Fragments arranged by size: 4689 672 475 420 378 336 68 16 FspI TGC'GCA Cuts at: 5560 5560 Size: 7054 HaeII r_GCGC'y Cuts at: 1678 6201 1678 Size: 4523 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5695 5727 5769 5801 1373 Size: 32 135 32 4123 32 42 32 2626 Fragments arranged by size: 4123 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 4398 4644 669 Size: 988 281 259 2201 246 3079 Fragments arranged by size: 3079 2201 988 281 259 246 HindIII A'AGCT_T Cuts at: 748 2757 2925 3093 3177 3261 3429 3765 3849 Size: 2009 168 168 84 84 168 336 84 Cuts at: 3849 4017 4101 748 Size: 168 84 3701 Fragments arranged by size: 3701 2009 336 168 168 168 168 84 84 84 84 HpaI GTT'AAC Cuts at: 2197 4644 2197 Size: 2447 4607 KpnI G_GTAC'C Cuts at: 4379 4379 Size: 7054 MfeI C'AATT_G Cuts at: 4653 4653 Size: 7054 MluI A'CGCG_T Cuts at: 6930 6930 Size: 7054 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6168 6352 1305 Size: 4863 184 2007 Fragments arranged by size: 4863 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2056 10 Size: 54 1425 343 224 5008 Fragments arranged by size: 5008 1425 343 224 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 3241 3831 5012 5371 5530 518 Size: 648 991 1084 590 1181 359 159 2042 Fragments arranged by size: 2042 1181 1084 991 648 590 359 159 MspA1I CmG'CkG Cuts at: 1606 4470 5147 6295 6540 1606 Size: 2864 677 1148 245 2120 Fragments arranged by size: 2864 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5563 Fragments arranged by size: 5563 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7054 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7054 NotI GC'GGCC_GC Cuts at: 4501 4501 Size: 7054 NspI r_CATG'y Cuts at: 1132 1229 2156 4412 5957 1132 Size: 97 927 2256 1545 2229 Fragments arranged by size: 2256 2229 1545 927 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7054 PciI A'CATG_T Cuts at: 2152 5953 2152 Size: 3801 3253 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7054 PmeI GTTT'AAAC Cuts at: 4358 4358 Size: 7054 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5123 5155 6636 6668 5123 Size: 32 1481 32 5509 Fragments arranged by size: 5509 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7054 PsiI TTA'TAA Cuts at: 3855 4624 3855 Size: 769 6285 PstI C_TGCA'G Cuts at: 830 3145 4406 830 Size: 2315 1261 3478 Fragments arranged by size: 3478 2315 1261 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7054 SalI G'TCGA_C Cuts at: 1936 4396 1936 Size: 2460 4594 SbfI CC_TGCA'GG Cuts at: 4406 4406 Size: 7054 ScaI AGT'ACT Cuts at: 5302 7014 5302 Size: 1712 5342 SfcI C'TryA_G Cuts at: 826 1045 3141 4402 5537 6218 6409 826 Size: 219 2096 1261 1135 681 191 1471 Fragments arranged by size: 2096 1471 1261 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7054 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7054 SmaI CCC'GGG Cuts at: 1872 4381 1872 Size: 2509 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5158 6059 6321 820 Size: 197 926 3215 901 262 1553 Fragments arranged by size: 3215 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4845 493 Size: 4352 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7054 SphI G_CATG'C Cuts at: 1132 4412 1132 Size: 3280 3774 SspI AAT'ATT Cuts at: 5 52 2229 4978 5 Size: 47 2177 2749 2081 Fragments arranged by size: 2749 2177 2081 47 StuI AGG'CCT Cuts at: 1517 2591 2603 3599 1517 Size: 1074 12 996 4972 Fragments arranged by size: 4972 1074 996 12 StyI C'CwwG_G Cuts at: 513 1065 2004 4493 513 Size: 552 939 2489 3074 Fragments arranged by size: 3074 2489 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5081 5098 5251 5436 5881 325 Size: 1255 3501 17 153 185 445 1498 Fragments arranged by size: 3501 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5300 7012 96 Size: 274 80 33 51 1443 3323 1712 138 Fragments arranged by size: 3323 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7014 7046 7014 Size: 32 7022 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7054 XbaI T'CTAG_A Cuts at: 4390 4390 Size: 7054 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7054 XmaI C'CCGG_G Cuts at: 1870 4379 1870 Size: 2509 4545 XmnI GAAnn'nnTTC Cuts at: 5183 5183 Size: 7054 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4862 276 Size: 53 83 186 936 3328 2468 Fragments arranged by size: 3328 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI