(Circular) (Six-base) MAPSORT of: pFN21AB7055.seq Check: 6396 from: 1 to: 6451 pFN21AB7055 6451 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 4261 278 Size: 53 83 186 936 1720 1005 2468 Fragments arranged by size: 2468 1720 1005 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2217 3794 1937 Size: 280 1577 4594 Fragments arranged by size: 4594 1577 280 Acc65I G'GTAC_C Cuts at: 3772 3772 Size: 6451 AclI AA'CG_TT Cuts at: 1563 4578 4951 1563 Size: 3015 373 3063 Fragments arranged by size: 3063 3015 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2517 2619 2979 3159 3339 4514 5898 6166 Size: 885 102 360 180 180 1175 1384 268 Cuts at: 6166 1632 Size: 1917 Fragments arranged by size: 1917 1384 1175 885 360 268 180 180 102 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6254 AflIII A'CryG_T Cuts at: 1203 5350 6327 1203 Size: 4147 977 1327 Fragments arranged by size: 4147 1327 977 AgeI A'CCGG_T Cuts at: 5282 5282 Size: 6451 AhdI GACnn_n'nnGTC Cuts at: 5180 5180 Size: 6451 AleI CACnn'nnGTG Cuts at: 1166 2304 1166 Size: 1138 5313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3708 1184 Size: 2524 3927 AlwNI CAG_nnn'CTG Cuts at: 2540 4194 5766 2540 Size: 1654 1572 3225 Fragments arranged by size: 3225 1654 1572 ApaLI G'TGCA_C Cuts at: 2544 4508 5664 2544 Size: 1964 1156 3331 Fragments arranged by size: 3331 1964 1156 ApoI r'AATT_y Cuts at: 1493 2638 3177 3760 3986 4138 6247 1493 Size: 1145 539 583 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 583 539 226 152 AseI AT'TA_AT Cuts at: 160 1032 5005 160 Size: 872 3973 1606 Fragments arranged by size: 3973 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 AvaI C'yCGr_G Cuts at: 1870 1943 3776 1870 Size: 73 1833 4545 Fragments arranged by size: 4545 1833 73 AvrII C'CTAG_G Cuts at: 2302 2302 Size: 6451 BamHI G'GATC_C Cuts at: 2570 3781 4148 2570 Size: 1211 367 4873 Fragments arranged by size: 4873 1211 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3772 5227 618 Size: 325 217 627 1985 1455 1842 Fragments arranged by size: 1985 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2784 3770 1391 Size: 1393 986 4072 Fragments arranged by size: 4072 1393 986 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2865 3735 928 Size: 971 966 870 3644 Fragments arranged by size: 3644 971 966 870 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4642 4676 1903 Size: 34 2705 34 3678 Fragments arranged by size: 3678 2705 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2941 3121 3301 3481 4343 5553 2941 Size: 180 180 180 862 1210 3839 Fragments arranged by size: 3839 1210 862 180 180 180 BclI T'GATC_A Cuts at: 1548 2227 3703 1548 Size: 679 1476 4296 Fragments arranged by size: 4296 1476 679 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5062 136 Size: 107 122 71 631 605 3390 1525 Fragments arranged by size: 3390 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6446 6446 Size: 6451 BlpI GC'TnA_GC Cuts at: 3868 3868 Size: 6451 Bme1580I G_kGCm'C Cuts at: 1790 2548 4512 5668 1790 Size: 758 1964 1156 2573 Fragments arranged by size: 2573 1964 1156 758 BmgBI CAC'GTC Cuts at: 1318 2680 1318 Size: 1362 5089 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4182 5140 442 Size: 416 53 83 399 24 2765 958 1753 Fragments arranged by size: 2765 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6451 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5111 1239 Size: 3872 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2418 3177 4576 5441 5739 2418 Size: 759 1399 865 298 3130 Fragments arranged by size: 3130 1399 865 759 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2340 5114 882 Size: 617 841 2774 2219 Fragments arranged by size: 2774 2219 841 617 BsaAI yAC'GTr Cuts at: 493 1204 2879 4242 493 Size: 711 1675 1363 2702 Fragments arranged by size: 2702 1675 1363 711 BsaBI GATnn'nnATC Cuts at: 6445 6445 Size: 6451 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3253 4258 4640 5275 Size: 53 83 186 936 1720 1005 382 635 Cuts at: 5275 275 Size: 1451 Fragments arranged by size: 1720 1451 1005 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4884 5282 5556 5703 6114 4884 Size: 398 274 147 411 5221 Fragments arranged by size: 5221 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6226 Fragments arranged by size: 6226 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6451 BseYI C'CCAG_C Cuts at: 5654 6150 5654 Size: 496 5955 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2709 1163 Size: 573 973 4905 Fragments arranged by size: 4905 973 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3901 4662 5690 1540 Size: 275 186 1900 761 1028 2301 Fragments arranged by size: 2301 1900 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2548 2784 3458 4512 4597 5668 2548 Size: 236 674 1054 85 1071 3331 Fragments arranged by size: 3331 1071 1054 674 236 85 BsmI GAATG_Cn' Cuts at: 2272 3962 4055 2272 Size: 1690 93 4668 Fragments arranged by size: 4668 1690 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3260 3260 Size: 6451 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2548 2784 3458 3770 4512 4597 5668 Size: 399 758 236 674 312 742 85 1071 Cuts at: 5668 1391 Size: 2174 Fragments arranged by size: 2174 1071 758 742 674 399 312 236 85 BspEI T'CCGG_A Cuts at: 6114 6114 Size: 6451 BspHI T'CATG_A Cuts at: 2230 3622 4340 6070 2230 Size: 1392 718 1730 2611 Fragments arranged by size: 2611 1730 1392 718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3806 844 Size: 2962 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4338 1394 Size: 429 2515 3507 Fragments arranged by size: 3507 2515 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4946 5120 65 Size: 1183 3698 174 1396 Fragments arranged by size: 3698 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5095 5282 1700 Size: 110 3285 187 2869 Fragments arranged by size: 3285 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2917 3277 96 Size: 2821 360 3270 Fragments arranged by size: 3270 2821 360 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6451 BssSI C'ACGA_G Cuts at: 4511 5523 4511 Size: 1012 5439 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6451 BstBI TT'CG_AA Cuts at: 5255 5255 Size: 6451 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6451 BstYI r'GATC_y Cuts at: 1972 2108 2570 3781 4148 4533 4550 6002 6088 Size: 136 462 1211 367 385 17 1452 86 Cuts at: 6088 6100 6446 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1211 462 385 367 346 136 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2902 513 Size: 552 1837 4062 Fragments arranged by size: 4062 1837 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5712 Fragments arranged by size: 5712 683 56 BtsI GCAGTG_nn' Cuts at: 3967 4761 4781 3967 Size: 794 20 5637 Fragments arranged by size: 5637 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6451 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6416 DraI TTT'AAA Cuts at: 3755 3755 Size: 6451 DraIII CAC_nnn'GTG Cuts at: 2567 2567 Size: 6451 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5458 809 Size: 507 4142 1802 Fragments arranged by size: 4142 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2842 3022 3202 Size: 54 1425 50 275 18 1012 180 180 Cuts at: 3202 3382 3562 3898 4787 6416 8 Size: 180 180 336 889 1629 43 Fragments arranged by size: 1629 1425 1012 889 336 275 180 180 180 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3898 1537 Size: 275 2086 4090 Fragments arranged by size: 4090 2086 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2955 3135 3315 4381 1353 Size: 1602 180 180 1066 3423 Fragments arranged by size: 3423 1602 1066 180 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5038 5412 5558 387 Size: 1064 138 3449 374 146 1280 Fragments arranged by size: 3449 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2782 2782 Size: 6451 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2517 2619 2979 3159 3339 4514 5111 Size: 393 885 102 360 180 180 1175 597 Cuts at: 5111 5898 6166 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1175 885 787 597 393 360 268 180 180 102 EcoNI CCTnn'n_nnAGG Cuts at: 2276 2276 Size: 6451 EcoRI G'AATT_C Cuts at: 3177 3760 4138 3177 Size: 583 378 5490 Fragments arranged by size: 5490 583 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2127 2127 Size: 6451 FspI TGC'GCA Cuts at: 4957 4957 Size: 6451 HaeII r_GCGC'y Cuts at: 1678 5598 1678 Size: 3920 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 2803 2835 3523 Size: 32 135 32 838 32 361 32 688 Cuts at: 3523 3542 3555 3574 5092 5124 5166 5198 1373 Size: 19 13 19 1518 32 42 32 2626 Fragments arranged by size: 2626 1518 838 688 361 135 42 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 2315 2614 2677 3795 4041 669 Size: 988 281 377 299 63 1118 246 3079 Fragments arranged by size: 3079 1118 988 377 299 281 246 63 HindIII A'AGCT_T Cuts at: 748 2529 748 Size: 1781 4670 HpaI GTT'AAC Cuts at: 2614 4041 2614 Size: 1427 5024 KpnI G_GTAC'C Cuts at: 3776 3776 Size: 6451 MfeI C'AATT_G Cuts at: 2096 4050 2096 Size: 1954 4497 MluI A'CGCG_T Cuts at: 6327 6327 Size: 6451 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2767 5565 5749 1305 Size: 1462 2798 184 2007 Fragments arranged by size: 2798 2007 1462 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3024 3204 3384 3564 10 Size: 54 1425 343 1192 180 180 180 2897 Fragments arranged by size: 2897 1425 1192 343 180 180 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 2304 4409 4768 4927 518 Size: 648 1138 2105 359 159 2042 Fragments arranged by size: 2105 2042 1138 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 3867 4544 5692 5937 1606 Size: 457 1804 677 1148 245 2120 Fragments arranged by size: 2120 1804 1148 677 457 245 NcoI C'CATG_G Cuts at: 513 1065 2902 513 Size: 552 1837 4062 Fragments arranged by size: 4062 1837 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6451 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6451 NotI GC'GGCC_GC Cuts at: 3898 3898 Size: 6451 NspI r_CATG'y Cuts at: 1132 1229 3809 5354 1132 Size: 97 2580 1545 2229 Fragments arranged by size: 2580 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6451 PciI A'CATG_T Cuts at: 5350 5350 Size: 6451 PflMI CCAn_nnn'nTGG Cuts at: 1108 2671 1108 Size: 1563 4888 PmeI GTTT'AAAC Cuts at: 3755 3755 Size: 6451 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4520 4552 6033 6065 4520 Size: 32 1481 32 4906 Fragments arranged by size: 4906 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6451 PsiI TTA'TAA Cuts at: 2331 4021 2331 Size: 1690 4761 PstI C_TGCA'G Cuts at: 830 3598 3803 830 Size: 2768 205 3478 Fragments arranged by size: 3478 2768 205 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6451 PvuII CAG'CTG Cuts at: 2063 2063 Size: 6451 SacI G_AGCT'C Cuts at: 2784 2784 Size: 6451 SalI G'TCGA_C Cuts at: 1936 3793 1936 Size: 1857 4594 SapI GCTCTTCn'nnn_ Cuts at: 3135 3135 Size: 6451 SbfI CC_TGCA'GG Cuts at: 3803 3803 Size: 6451 ScaI AGT'ACT Cuts at: 4699 6411 4699 Size: 1712 4739 SexAI A'CCwGG_T Cuts at: 2988 3168 3348 2988 Size: 180 180 6091 Fragments arranged by size: 6091 180 180 SfcI C'TryA_G Cuts at: 826 1045 3594 3747 3799 4934 5615 5806 826 Size: 219 2549 153 52 1135 681 191 1471 Fragments arranged by size: 2549 1471 1135 681 219 191 153 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6451 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6451 SmaI CCC'GGG Cuts at: 1872 3778 1872 Size: 1906 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2433 3192 4555 5456 5718 820 Size: 197 926 490 759 1363 901 262 1553 Fragments arranged by size: 1553 1363 926 901 759 490 262 197 SnaBI TAC'GTA Cuts at: 493 4242 493 Size: 3749 2702 SpeI A'CTAG_T Cuts at: 152 2605 152 Size: 2453 3998 SphI G_CATG'C Cuts at: 1132 3809 1132 Size: 2677 3774 SspI AAT'ATT Cuts at: 5 52 3670 4375 5 Size: 47 3618 705 2081 Fragments arranged by size: 3618 2081 705 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6451 StyI C'CwwG_G Cuts at: 513 1065 2302 2902 3890 513 Size: 552 1237 600 988 3074 Fragments arranged by size: 3074 1237 988 600 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4478 4495 4648 4833 5278 325 Size: 1255 2898 17 153 185 445 1498 Fragments arranged by size: 2898 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2917 3059 3239 Size: 274 80 33 51 1443 940 142 180 Cuts at: 3239 3277 3419 4697 6409 96 Size: 38 142 1278 1712 138 Fragments arranged by size: 1712 1443 1278 940 274 180 142 142 138 80 51 38 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6411 6443 6411 Size: 32 6419 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6451 XbaI T'CTAG_A Cuts at: 3787 3787 Size: 6451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2123 2147 2411 2123 Size: 24 264 6163 Fragments arranged by size: 6163 264 24 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6451 XmaI C'CCGG_G Cuts at: 1870 3776 1870 Size: 1906 4545 XmnI GAAnn'nnTTC Cuts at: 4580 4580 Size: 6451 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 4259 276 Size: 53 83 186 936 1720 1005 2468 Fragments arranged by size: 2468 1720 1005 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI