Restriction map for
pFN21AB7052
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspHI BspLU11I
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HincII HindII
HpaI KpnI KspI MaeII MamI
MfeI MluI MseI MunI MvnI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 113 TCCGGA T^CCGGA
AcsI 310 GAATTT [GA]^AATT[CT]
ApoI 310 GAATTT [GA]^AATT[CT]
AvaI 10 CCCGAG C^[CT]CG[GA]G
BalI 1464 TGGCCA TGG^CCA
BbsI 532 GAAGAC GAAGAC(2/6)
BpuAI 532 GAAGAC GAAGAC(2/6)
BsaI 1533 GGTCTC GGTCTC(1/5)
BsaWI 113 TCCGGA [AT]^CCGG[AT]
BseAI 113 TCCGGA T^CCGGA
BsmAI 1534 GTCTC GTCTC(1/5)
BsoBI 10 CCCGAG C^[CT]CG[GA]G
BspDI 928 ATCGAT AT^CGAT
BspEI 113 TCCGGA T^CCGGA
BsrBI 1020 CCGCTC CCGCTC(-3/-3)
ClaI 928 ATCGAT AT^CGAT
EarI 835 GAAGAG CTCTTC(1/4)
HgaI 117 GACGC GACGC(5/10)
Ksp632I 835 GAAGAG CTCTTC(1/4)
MaeIII 101 GTGAC ^GT[ACGT]AC
MluNI 1464 TGGCCA TGG^CCA
MroI 113 TCCGGA T^CCGGA
MscI 1464 TGGCCA TGG^CCA
NheI 153 GCTAGC G^CTAGC
PleI 1609 GAGTC GAGTC(4/5)
SacI 55 GAGCTC GAGCT^C
SmlI 1249 CTCAAG C^T[CT][GA]AG
Tsp45I 101 GTGAC ^GT[GC]AC
Tsp509I 311 AATT ^AATT
XcmI 1460 CCAATGGCCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 298 GGCGCC G[GA]^CG[CT]C
1382 GGCGCC
AlwNI 481 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
773 CAGGAACTG
Asp700I 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
615 GAAAGCTTTC
AspHI 55 GAGCTC G[AT]GC[AT]^C
361 GTGCTC
AviII 1010 TGCGCA TGC^GCA
1370 TGCGCA
BbeI 298 GGCGCC GGCGC^C
1382 GGCGCC
BglI 83 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
576 GCCACCTCGGC
BsaHI 298 GGCGCC G[GA]^CG[CT]C
1382 GGCGCC
BsiHKAI 55 GAGCTC G[AT]GC[AT]^C
361 GTGCTC
BspMI 1265 ACCTGC ACCTGC(4/8)
1331 ACCTGC
BsrFI 256 GCCGGT [GA]^CCGG[CT]
1280 ACCGGC
BstYI 386 AGATCC [GA]^GATC[CT]
1169 AGATCC
Cfr10I 256 GCCGGT [GA]^CCGG[CT]
1280 ACCGGC
EaeI 236 CGGCCA [CT]^GGCC[GA]
1464 TGGCCA
EcoNI 766 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
960 CCTGCACCAGG
FspI 1010 TGCGCA TGC^GCA
1370 TGCGCA
Hin1I 298 GGCGCC G[GA]^CG[CT]C
1382 GGCGCC
HindIII 181 AAGCTT A^AGCTT
617 AAGCTT
KasI 298 GGCGCC G^GCGCC
1382 GGCGCC
MflI 386 AGATCC [GA]^GATC[CT]
1169 AGATCC
MslI 46 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
94 CACCTATGTG
NarI 298 GGCGCC GG^CGCC
1382 GGCGCC
NcoI 1065 CCATGG C^CATGG
1604 CCATGG
PflMI 480 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
856 CCAGCCTATGG
PpuMI 795 GGGTCCC [GA]G^G[AT]CC[CT]
1096 AGGACCC
PstI 421 CTGCAG CTGCA^G
1333 CTGCAG
PvuII 85 CAGCTG CAG^CTG
356 CAGCTG
SfoI 298 GGCGCC GGC^GCC
1382 GGCGCC
StuI 491 AGGCCT AGG^CCT
984 AGGCCT
Van91I 480 CCAGTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
856 CCAGCCTATGG
XhoII 386 AGATCC [GA]^GATC[CT]
1169 AGATCC
XmnI 180 GAAGCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
615 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AluI AlwI AocI ApaI
AvaII BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MboI
MboII MnlI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
TspRI TthHB8I