(Circular) (Six-base) MAPSORT of: pFN21AB7052.seq Check: 7204 from: 1 to: 6322 pFN21AB7052 6322 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3279 3345 3279 Size: 66 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4132 278 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3665 1937 Size: 1728 4594 Acc65I G'GTAC_C Cuts at: 3643 3643 Size: 6322 AclI AA'CG_TT Cuts at: 1563 4449 4822 1563 Size: 2886 373 3063 Fragments arranged by size: 3063 2886 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3191 3621 4385 5769 6037 1632 Size: 1559 430 764 1384 268 1917 Fragments arranged by size: 1917 1559 1384 764 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6125 AflIII A'CryG_T Cuts at: 1203 5221 6198 1203 Size: 4018 977 1327 Fragments arranged by size: 4018 1327 977 AgeI A'CCGG_T Cuts at: 5153 5153 Size: 6322 AhdI GACnn_n'nnGTC Cuts at: 5051 5051 Size: 6322 AleI CACnn'nnGTG Cuts at: 1166 2055 2103 1166 Size: 889 48 5385 Fragments arranged by size: 5385 889 48 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6322 AlwNI CAG_nnn'CTG Cuts at: 2491 2783 4065 5637 2491 Size: 292 1282 1572 3176 Fragments arranged by size: 3176 1572 1282 292 ApaI G_GGCC'C Cuts at: 2892 3534 3555 2892 Size: 642 21 5659 Fragments arranged by size: 5659 642 21 ApaLI G'TGCA_C Cuts at: 4379 5535 4379 Size: 1156 5166 ApoI r'AATT_y Cuts at: 1493 2315 3631 3857 4009 6118 1493 Size: 822 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 822 226 152 AseI AT'TA_AT Cuts at: 160 1032 4876 160 Size: 872 3844 1606 Fragments arranged by size: 3844 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 AvaI C'yCGr_G Cuts at: 1870 1943 2015 3647 1870 Size: 73 72 1632 4545 Fragments arranged by size: 4545 1632 73 72 BamHI G'GATC_C Cuts at: 3652 4019 3652 Size: 367 5955 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2303 2554 2671 2983 3387 Size: 325 217 627 516 251 117 312 404 Cuts at: 3387 3580 3605 3643 5098 618 Size: 193 25 38 1455 1842 Fragments arranged by size: 1842 1455 627 516 404 325 312 251 217 193 117 38 25 BanII G_rGCy'C Cuts at: 1391 2015 2064 2482 2892 2918 3534 3555 3641 Size: 624 49 418 410 26 616 21 86 Cuts at: 3641 1391 Size: 4072 Fragments arranged by size: 4072 624 616 418 410 86 49 26 21 BbeI G_GCGC'C Cuts at: 2307 3391 2307 Size: 1084 5238 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2544 928 Size: 971 645 4706 Fragments arranged by size: 4706 971 645 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4513 4547 1903 Size: 34 2576 34 3678 Fragments arranged by size: 3678 2576 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4214 5424 4214 Size: 1210 5112 BclI T'GATC_A Cuts at: 1548 1548 Size: 6322 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2094 2587 4933 Size: 107 122 71 631 605 422 493 2346 Cuts at: 4933 136 Size: 1525 Fragments arranged by size: 2346 1525 631 605 493 422 122 107 71 BglII A'GATC_T Cuts at: 6317 6317 Size: 6322 BlpI GC'TnA_GC Cuts at: 3739 3739 Size: 6322 Bme1580I G_kGCm'C Cuts at: 1790 2559 2676 2685 2892 3050 3534 3555 3583 Size: 769 117 9 207 158 484 21 28 Cuts at: 3583 4383 5539 1790 Size: 800 1156 2573 Fragments arranged by size: 2573 1156 800 769 484 207 158 117 28 21 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6322 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2256 3537 4053 Size: 416 53 83 399 24 839 1281 516 Cuts at: 4053 5011 442 Size: 958 1753 Fragments arranged by size: 1753 1281 958 839 516 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2162 1056 Size: 1106 5216 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 6322 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2408 2513 2541 3389 4982 1239 Size: 1115 54 105 28 848 1593 2579 Fragments arranged by size: 2579 1593 1115 848 105 54 28 Bpu10I CC'TnA_GC Cuts at: 2066 2957 2066 Size: 891 5431 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3239 4447 5312 5610 3239 Size: 1208 865 298 3951 Fragments arranged by size: 3951 1208 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3544 4985 882 Size: 617 2045 1441 2219 Fragments arranged by size: 2219 2045 1441 617 BsaAI yAC'GTr Cuts at: 493 1204 4113 493 Size: 711 2909 2702 Fragments arranged by size: 2909 2702 711 BsaBI GATnn'nnATC Cuts at: 6316 6316 Size: 6322 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2304 3388 4129 4511 Size: 53 83 186 936 771 1084 741 382 Cuts at: 4511 5146 275 Size: 635 1451 Fragments arranged by size: 1451 1084 936 771 741 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 4755 5153 5427 5574 5985 2118 Size: 2637 398 274 147 411 2455 Fragments arranged by size: 2637 2455 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2381 2411 2945 2975 1178 Size: 30 165 30 978 30 534 30 4525 Fragments arranged by size: 4525 978 534 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2720 2723 2726 2858 2921 3584 1186 Size: 1534 3 3 132 63 663 3924 Fragments arranged by size: 3924 1534 663 132 63 3 3 BseYI C'CCAG_C Cuts at: 2092 2450 2860 2911 3226 5525 6021 2092 Size: 358 410 51 315 2299 496 2393 Fragments arranged by size: 2393 2299 496 410 358 315 51 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2951 3224 3257 1163 Size: 573 1215 273 33 4228 Fragments arranged by size: 4228 1215 573 273 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3772 4533 5561 1540 Size: 275 186 1771 761 1028 2301 Fragments arranged by size: 2301 1771 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2064 2370 4383 4468 5539 2064 Size: 306 2013 85 1071 2847 Fragments arranged by size: 2847 2013 1071 306 85 BsmI GAATG_Cn' Cuts at: 3833 3926 3833 Size: 93 6229 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2064 2370 2482 2559 2676 2685 Size: 399 225 49 306 112 77 117 9 Cuts at: 2685 2892 2918 3050 3534 3555 3583 3641 4383 Size: 207 26 132 484 21 28 58 742 Cuts at: 4383 4468 5539 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 306 225 207 132 117 112 85 77 58 49 28 26 21 9 BspEI T'CCGG_A Cuts at: 2118 5985 2118 Size: 3867 2455 BspHI T'CATG_A Cuts at: 4211 5941 4211 Size: 1730 4592 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3279 3345 3677 844 Size: 2435 66 332 3489 Fragments arranged by size: 3489 2435 332 66 BsrBI CCG'CTC Cuts at: 1394 1823 3027 4209 1394 Size: 429 1204 1182 3507 Fragments arranged by size: 3507 1204 1182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4817 4991 65 Size: 1183 3569 174 1396 Fragments arranged by size: 3569 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2261 3285 4966 5153 1700 Size: 110 451 1024 1681 187 2869 Fragments arranged by size: 2869 1681 1024 451 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6322 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6322 BssSI C'ACGA_G Cuts at: 4382 5394 4382 Size: 1012 5310 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6322 BstBI TT'CG_AA Cuts at: 5126 5126 Size: 6322 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6322 BstYI r'GATC_y Cuts at: 1972 2391 3174 3652 4019 4404 4421 5873 5959 Size: 419 783 478 367 385 17 1452 86 Cuts at: 5959 5971 6317 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 783 478 419 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2667 2796 3039 2667 Size: 129 243 5950 Fragments arranged by size: 5950 243 129 BtgI C'CryG_G Cuts at: 513 1065 2004 2511 3070 3609 513 Size: 552 939 507 559 539 3226 Fragments arranged by size: 3226 939 559 552 539 507 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2389 487 Size: 683 56 1163 4420 Fragments arranged by size: 4420 1163 683 56 BtsI GCAGTG_nn' Cuts at: 2062 2440 2743 2752 3220 3838 4632 4652 2062 Size: 378 303 9 468 618 794 20 3732 Fragments arranged by size: 3732 794 618 468 378 303 20 9 ClaI AT'CG_AT Cuts at: 1554 2934 1554 Size: 1380 4942 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6287 DraI TTT'AAA Cuts at: 3626 3626 Size: 6322 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5329 809 Size: 507 4013 1802 Fragments arranged by size: 4013 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2241 3469 3769 Size: 54 1425 50 275 18 411 1228 300 Cuts at: 3769 4658 6287 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1228 889 411 300 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3769 1537 Size: 275 1957 4090 Fragments arranged by size: 4090 1957 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2835 4252 1353 Size: 1482 1417 3423 Fragments arranged by size: 3423 1482 1417 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2223 2562 3476 4909 5283 5429 Size: 1064 138 634 339 914 1433 374 146 Cuts at: 5429 387 Size: 1280 Fragments arranged by size: 1433 1280 1064 914 634 374 339 146 138 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 6322 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2408 2513 2541 3191 3389 3621 Size: 393 722 54 105 28 650 198 232 Cuts at: 3621 4385 4982 5769 6037 1239 Size: 764 597 787 268 1524 Fragments arranged by size: 1524 787 764 722 650 597 393 268 232 198 105 54 28 EcoNI CCTnn'n_nnAGG Cuts at: 2775 2969 2775 Size: 194 6128 EcoO109I rG'GnC_Cy Cuts at: 2128 2801 2889 3102 3531 3551 3552 2128 Size: 673 88 213 429 20 1 4898 Fragments arranged by size: 4898 673 429 213 88 20 1 EcoRI G'AATT_C Cuts at: 3631 4009 3631 Size: 378 5944 FspI TGC'GCA Cuts at: 3017 3377 4828 3017 Size: 360 1451 4511 Fragments arranged by size: 4511 1451 360 FspAI rTGC'GCAy Cuts at: 3377 3377 Size: 6322 HaeII r_GCGC'y Cuts at: 1678 2089 2307 2352 3205 3346 3391 5469 1678 Size: 411 218 45 853 141 45 2078 2531 Fragments arranged by size: 2531 2078 853 411 218 141 45 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4963 4995 5037 5069 1373 Size: 32 135 32 3391 32 42 32 2626 Fragments arranged by size: 3391 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3666 3912 669 Size: 988 281 1728 246 3079 Fragments arranged by size: 3079 1728 988 281 246 HindIII A'AGCT_T Cuts at: 748 2186 2622 748 Size: 1438 436 4448 Fragments arranged by size: 4448 1438 436 HpaI GTT'AAC Cuts at: 3912 3912 Size: 6322 KasI G'GCGC_C Cuts at: 2303 3387 2303 Size: 1084 5238 KpnI G_GTAC'C Cuts at: 3647 3647 Size: 6322 MfeI C'AATT_G Cuts at: 3921 3921 Size: 6322 MluI A'CGCG_T Cuts at: 6198 6198 Size: 6322 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5436 5620 1305 Size: 4131 184 2007 Fragments arranged by size: 4131 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3471 10 Size: 54 1425 343 1639 2861 Fragments arranged by size: 2861 1639 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2055 2103 4280 4639 4798 518 Size: 648 889 48 2177 359 159 2042 Fragments arranged by size: 2177 2042 889 648 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2092 2363 2896 3434 3449 3738 4415 5563 Size: 486 271 533 538 15 289 677 1148 Cuts at: 5563 5808 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 538 533 486 289 271 245 15 NarI GG'CG_CC Cuts at: 2304 3388 2304 Size: 1084 5238 NcoI C'CATG_G Cuts at: 513 1065 2004 3070 3609 513 Size: 552 939 1066 539 3226 Fragments arranged by size: 3226 1066 939 552 539 NdeI CA'TA_TG Cuts at: 387 387 Size: 6322 NheI G'CTAG_C Cuts at: 1052 2158 1052 Size: 1106 5216 NotI GC'GGCC_GC Cuts at: 3769 3769 Size: 6322 NspI r_CATG'y Cuts at: 1132 1229 2359 3184 3268 3680 5225 1132 Size: 97 1130 825 84 412 1545 2229 Fragments arranged by size: 2229 1545 1130 825 412 97 84 PasI CC'CwG_GG Cuts at: 1348 3548 1348 Size: 2200 4122 PciI A'CATG_T Cuts at: 5221 5221 Size: 6322 PflMI CCAn_nnn'nTGG Cuts at: 1108 2491 2867 1108 Size: 1383 376 4563 Fragments arranged by size: 4563 1383 376 PfoI T'CCnGG_A Cuts at: 2043 2043 Size: 6322 PmeI GTTT'AAAC Cuts at: 3626 3626 Size: 6322 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4391 4423 5904 5936 4391 Size: 32 1481 32 4777 Fragments arranged by size: 4777 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2801 3102 2801 Size: 301 6021 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6322 PsiI TTA'TAA Cuts at: 3892 3892 Size: 6322 PspOMI G'GGCC_C Cuts at: 2888 3530 3551 2888 Size: 642 21 5659 Fragments arranged by size: 5659 642 21 PstI C_TGCA'G Cuts at: 830 2430 3342 3674 830 Size: 1600 912 332 3478 Fragments arranged by size: 3478 1600 912 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6322 PvuII CAG'CTG Cuts at: 2092 2363 2092 Size: 271 6051 SacI G_AGCT'C Cuts at: 2064 2064 Size: 6322 SalI G'TCGA_C Cuts at: 1936 3664 1936 Size: 1728 4594 SanDI GG'GwC_CC Cuts at: 2801 2801 Size: 6322 SbfI CC_TGCA'GG Cuts at: 3674 3674 Size: 6322 ScaI AGT'ACT Cuts at: 4570 6282 4570 Size: 1712 4610 SfcI C'TryA_G Cuts at: 826 1045 2167 2426 2764 3338 3670 4805 5486 Size: 219 1122 259 338 574 332 1135 681 Cuts at: 5486 5677 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1122 681 574 338 332 259 219 191 SfoI GGC'GCC Cuts at: 2305 3389 2305 Size: 1084 5238 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6322 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6322 SmaI CCC'GGG Cuts at: 1872 3649 1872 Size: 1777 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3254 4426 5327 5589 820 Size: 197 926 1311 1172 901 262 1553 Fragments arranged by size: 1553 1311 1172 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4113 493 Size: 3620 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6322 SphI G_CATG'C Cuts at: 1132 3680 1132 Size: 2548 3774 SspI AAT'ATT Cuts at: 5 52 4246 5 Size: 47 4194 2081 Fragments arranged by size: 4194 2081 47 StuI AGG'CCT Cuts at: 1517 2498 2991 1517 Size: 981 493 4848 Fragments arranged by size: 4848 981 493 StyI C'CwwG_G Cuts at: 513 1065 2004 2637 3070 3324 3609 3761 513 Size: 552 939 633 433 254 285 152 3074 Fragments arranged by size: 3074 939 633 552 433 285 254 152 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2159 3598 4349 4366 4519 4704 5149 Size: 1255 579 1439 751 17 153 185 445 Cuts at: 5149 325 Size: 1498 Fragments arranged by size: 1498 1439 1255 751 579 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4568 6280 96 Size: 274 80 33 51 1443 2591 1712 138 Fragments arranged by size: 2591 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2381 2413 6282 6314 2381 Size: 32 3869 32 2389 Fragments arranged by size: 3869 2389 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6322 XbaI T'CTAG_A Cuts at: 3658 3658 Size: 6322 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3472 3472 Size: 6322 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6322 XmaI C'CCGG_G Cuts at: 1870 3647 1870 Size: 1777 4545 XmnI GAAnn'nnTTC Cuts at: 2189 2624 4451 2189 Size: 435 1827 4060 Fragments arranged by size: 4060 1827 435 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4130 276 Size: 53 83 186 936 2596 2468 Fragments arranged by size: 2596 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FalI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SapI SexAI SfiI SrfI SwaI